LOCUS       sequence-212545-        2631 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Upstream homology arm, Pair of HAs spans yckA and yckB, which share 
            homology with ABC transporters in B. subtilis.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2631)
  AUTHORS   Wicke N, Radford D, Verrone V, Wipat A, French CE
  TITLE     BacilloFlex: A modular DNA assembly toolkit for Bacillus subtilis 
            synthetic biology
  JOURNAL   bioRxiv 185108
REFERENCE   2  (bases 1 to 2631)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            185108"
COMMENT     Sequence Label: pTU1-A-yckABU
FEATURES             Location/Qualifiers
     source          1..2631
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(579..596)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(750..1338)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(830..849)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(1509..2300,2301..2369))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     2132..2151
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(2370..2474)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     2542..2560
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     terminator      complement(2564..2607)
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     misc_feature    2610..2629
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that start with ""ATG"""
ORIGIN
        1 cgtctcaatc tggaatcgta gtcgctacat caggtacgct ctatcccact tcttatcacg
       61 atactgactc tggctctgat aagctgacgg gctacgaggt tgaagttgtt cgtgaagcgg
      121 caaaacgctt ggggctgaag gttgagttca aggagatggg cattgacggg atgctgacag
      181 cggtgaacag cggacaggtt gacgcagccg cgaatgacat cgatgtgacg aaggaccgtg
      241 aggagaagtt cgcattttcc actccatata aatattcata cggcacggca attgtccgaa
      301 aagacgattt atcagggatt aaaacgctca aggatttaaa agggaagaaa gcggctggcg
      361 cggctacaac cgtctacatg gaggtcgcga gaaaatacgg cgccaaagaa gtgatttatg
      421 ataatgcgac gaatgagcaa tatttaaaag atgttgccaa cggacgcact gacgtcattt
      481 taaacgacta ttacctgcag acacttgcgc tcgcggcatt cccggatctc aacattacaa
      541 ttcatcctga catcaatgcc agagacgctg caggcttcct cgctcactga ctcgctgcgc
      601 tcggtcgttc ggctgcggcg agcggtatca gctcactcaa aggcggtaat acggttatcc
      661 acagaatcag gggataacgc aggaaagaac atgtgagcaa aaggccagca aaaggccagg
      721 aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat
      781 cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag
      841 gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga
      901 tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg
      961 tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt
     1021 cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac
     1081 gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc
     1141 ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag gacagtattt
     1201 ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc
     1261 ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc
     1321 agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg
     1381 aacgaaaact cacgttaagg gattttggtc atgagattat caaaaaggat cttcacctag
     1441 atccttttaa attaaaaatg aagttttaaa tcaatctaaa gtatatatga gtaaacttgg
     1501 tctgacagtt accaatgctt aatcagtgag gcacctatct cagcgatctg tctatttcgt
     1561 tcatccatag ttgcctgact ccccgtcgtg tagataacta cgatacggga gggcttacca
     1621 tctggcccca gtgctgcaat gataccgcga gagccacgct caccggctcc agatttatca
     1681 gcaataaacc agccagccgg aagggccgag cgcagaagtg gtcctgcaac tttatccgcc
     1741 tccatccagt ctattaattg ttgccgggaa gctagagtaa gtagttcgcc agttaatagt
     1801 ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg
     1861 gcttcattca gctccggttc ccaacgatca aggcgagtta catgatcccc catgttgtgc
     1921 aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg
     1981 ttatcactca tggttatggc agcactgcat aattctctta ctgtcatgcc atccgtaaga
     2041 tgcttttctg tgactggtga gtactcaacc aagtcattct gagaatagtg tatgcggcga
     2101 ccgagttgct cttgcccggc gtcaatacgg gataataccg cgccacatag cagaacttta
     2161 aaagtgctca tcattggaaa acgttcttcg gggcgaaaac tctcaaggat cttaccgctg
     2221 ttgagatcca gttcgatgta acccactcgt gcacccaact gatcttcagc atcttttact
     2281 ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata
     2341 agggcgacac ggaaatgttg aatactcata ctcttccttt ttcaatatta ttgaagcatt
     2401 tatcagggtt attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa
     2461 ataggggttc cgcgcacatt tccccgaaaa gtgccacctg acgtctaaga aaccattatt
     2521 atcatgacat taacctataa aaataggcgt atcacgaggc agaatttcag ataaaaaaaa
     2581 tccttagctt tcgctaagga tgatttctgg aattcgcggc cgcttctaga a
//
