LOCUS       sequence-212547-        2510 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Downstream homology arm, SpsC is involved in spore coat 
            polysaccharide synthesis.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2510)
  AUTHORS   Wicke N, Radford D, Verrone V, Wipat A, French CE
  TITLE     BacilloFlex: A modular DNA assembly toolkit for Bacillus subtilis 
            synthetic biology
  JOURNAL   bioRxiv 185108
REFERENCE   2  (bases 1 to 2510)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            185108"
COMMENT     Sequence Label: pTU1-D-spsCD
FEATURES             Location/Qualifiers
     source          1..2510
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(458..475)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(629..1217)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(709..728)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(1388..2179,2180..2248))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     2011..2030
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(2249..2353)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     2421..2439
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     terminator      complement(2443..2486)
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     misc_feature    2489..2508
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that start with ""ATG"""
ORIGIN
        1 cgtctcagaa gagatggata tgtttgattt gcaggcggcc ctcggactgt catcagctga
       61 aacggctgga tgatatgcag aaacggagag aagagattgc cggccgctat caaaccgctt
      121 ttcagcagat tcctgggctg atcacgccgt ttgtccatga tgacggcagg cacgcctggc
      181 atctatatgt tctgcaagtt gatgaaaaaa aggccggggt tacgcgcagc gaaatgatta
      241 cggctttaaa ggatgaatac aatatcggca ctagcgttca ttttattcca gtacacattc
      301 atccgtatta tcaaaagcaa tttggatata aggaagcgga ctttccgaat gccatgaatt
      361 actataaacg aaccctttct ctcccgctct atccatcaat gtctgacgat gatgtagatg
      421 atgtcattga agctgttaga gagacgctgc aggcttcctc gctcactgac tcgctgcgct
      481 cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa ggcggtaata cggttatcca
      541 cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga
      601 accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc
      661 acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg
      721 cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat
      781 acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt
      841 atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc
      901 agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg
      961 acttatcgcc actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg
     1021 gtgctacaga gttcttgaag tggtggccta actacggcta cactagaagg acagtatttg
     1081 gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg
     1141 gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca
     1201 gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga
     1261 acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc ttcacctaga
     1321 tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag taaacttggt
     1381 ctgacagtta ccaatgctta atcagtgagg cacctatctc agcgatctgt ctatttcgtt
     1441 catccatagt tgcctgactc cccgtcgtgt agataactac gatacgggag ggcttaccat
     1501 ctggccccag tgctgcaatg ataccgcgag agccacgctc accggctcca gatttatcag
     1561 caataaacca gccagccgga agggccgagc gcagaagtgg tcctgcaact ttatccgcct
     1621 ccatccagtc tattaattgt tgccgggaag ctagagtaag tagttcgcca gttaatagtt
     1681 tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg
     1741 cttcattcag ctccggttcc caacgatcaa ggcgagttac atgatccccc atgttgtgca
     1801 aaaaagcggt tagctccttc ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt
     1861 tatcactcat ggttatggca gcactgcata attctcttac tgtcatgcca tccgtaagat
     1921 gcttttctgt gactggtgag tactcaacca agtcattctg agaatagtgt atgcggcgac
     1981 cgagttgctc ttgcccggcg tcaatacggg ataataccgc gccacatagc agaactttaa
     2041 aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt
     2101 tgagatccag ttcgatgtaa cccactcgtg cacccaactg atcttcagca tcttttactt
     2161 tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa
     2221 gggcgacacg gaaatgttga atactcatac tcttcctttt tcaatattat tgaagcattt
     2281 atcagggtta ttgtctcatg agcggataca tatttgaatg tatttagaaa aataaacaaa
     2341 taggggttcc gcgcacattt ccccgaaaag tgccacctga cgtctaagaa accattatta
     2401 tcatgacatt aacctataaa aataggcgta tcacgaggca gaatttcaga taaaaaaaat
     2461 ccttagcttt cgctaaggat gatttctgga attcgcggcc gcttctagaa
//
