LOCUS       sequence-212548-        2655 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Downstream homology arm, Pair of HAs spans yckA and yckB, which 
            share homology with ABC transporters in B. subtilis.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2655)
  AUTHORS   Wicke N, Radford D, Verrone V, Wipat A, French CE
  TITLE     BacilloFlex: A modular DNA assembly toolkit for Bacillus subtilis 
            synthetic biology
  JOURNAL   bioRxiv 185108
REFERENCE   2  (bases 1 to 2655)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            185108"
COMMENT     Sequence Label: pTU1-D-yckABD
FEATURES             Location/Qualifiers
     source          1..2655
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(603..620)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(774..1362)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(854..873)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(1533..2324,2325..2393))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     2156..2175
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(2394..2498)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     2566..2584
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     terminator      complement(2588..2631)
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     misc_feature    2634..2653
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that start with ""ATG"""
ORIGIN
        1 cgtctcagaa gccattgaat cgttcccgtt atgtcataaa aggcattggt tatacactgc
       61 tcatctcttt tgtaagcatg tttgcgggaa ctgtgatcgg cctgttcata tcactcgcca
      121 gaatgtctaa acttgctttg ttaagatggc cggcgaagct ctatatctca tttatgcgag
      181 gcgttccgat tttggtgatt ttgtttatcc tttatttcgg gtttccttat atcggcattg
      241 aattttccgc tgtcacggct gctttaatcg gctttagcct aaacagcgcc gcctacattg
      301 cggaaattaa ccgttcagcc atttcatccg tcgaaaaagg acagtgggaa gccgcatcct
      361 cgctgggtct ttcctattgg cagacgatga ggggcatcat tttgccgcaa tcgatacgta
      421 ttgcgctgcc tccgcttgcc aatgtattgc tcgacttgat caaggcgtcc tctctcgctg
      481 cgatgattac agtgccggaa ttgctccagc atgcgaaaat catcgggggc agagagtttg
      541 attatatgac catgtatatt ctgaccgctc tcatttattg ttagagagac gctgcaggct
      601 tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt atcagctcac
      661 tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga
      721 gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat
      781 aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac
      841 ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct
      901 gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg
      961 ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg
     1021 ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt
     1081 cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg
     1141 attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac
     1201 ggctacacta gaaggacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga
     1261 aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt
     1321 gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt
     1381 tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga
     1441 ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc
     1501 taaagtatat atgagtaaac ttggtctgac agttaccaat gcttaatcag tgaggcacct
     1561 atctcagcga tctgtctatt tcgttcatcc atagttgcct gactccccgt cgtgtagata
     1621 actacgatac gggagggctt accatctggc cccagtgctg caatgatacc gcgagagcca
     1681 cgctcaccgg ctccagattt atcagcaata aaccagccag ccggaagggc cgagcgcaga
     1741 agtggtcctg caactttatc cgcctccatc cagtctatta attgttgccg ggaagctaga
     1801 gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg ccattgctac aggcatcgtg
     1861 gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg gttcccaacg atcaaggcga
     1921 gttacatgat cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt
     1981 gtcagaagta agttggccgc agtgttatca ctcatggtta tggcagcact gcataattct
     2041 cttactgtca tgccatccgt aagatgcttt tctgtgactg gtgagtactc aaccaagtca
     2101 ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat acgggataat
     2161 accgcgccac atagcagaac tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga
     2221 aaactctcaa ggatcttacc gctgttgaga tccagttcga tgtaacccac tcgtgcaccc
     2281 aactgatctt cagcatcttt tactttcacc agcgtttctg ggtgagcaaa aacaggaagg
     2341 caaaatgccg caaaaaaggg aataagggcg acacggaaat gttgaatact catactcttc
     2401 ctttttcaat attattgaag catttatcag ggttattgtc tcatgagcgg atacatattt
     2461 gaatgtattt agaaaaataa acaaataggg gttccgcgca catttccccg aaaagtgcca
     2521 cctgacgtct aagaaaccat tattatcatg acattaacct ataaaaatag gcgtatcacg
     2581 aggcagaatt tcagataaaa aaaatcctta gctttcgcta aggatgattt ctggaattcg
     2641 cggccgcttc tagaa
//
