LOCUS       sequence-212559-        9846 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses the C. elegans acy-1 cDNA in body wall muscle.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9846)
  AUTHORS   Reynolds NK, Schade MA, Miller KG
  TITLE     Convergent, RIC-8-dependent Galpha signaling pathways in the 
            Caenorhabditis elegans synaptic signaling network.
  JOURNAL   Genetics. 2005 Feb;169(2):651-70. doi: 10.1534/genetics.104.031286. 
            Epub 2004 Oct 16.
  PUBMED    15489511
REFERENCE   2  (bases 1 to 9846)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1534/genetics.104.031286"; journalName: "Genetics"; date: 
            "2005-02"; volume: "169"; issue: "2"; pages: "651-70"
COMMENT     Sequence Label: KG#80
FEATURES             Location/Qualifiers
     source          1..9846
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(7292..7314)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     7414..7433
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(7511..7529)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        7597..7701
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7702..7770,7771..8562)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7920..7939)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8733..9321
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9222..9241
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     9475..9492
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    9609..9630
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(9645..9650,9651..9668,9669..9675)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9683..9699
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     9688..9710
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     9707..9723
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     9707..9723
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 tgaaataagc ttgggctgca ggtcggctat aataagttct tgaataaaat aattttcccg
       61 acaaaacatg agtatttctt tcgaaaataa aagtgcaggc taattagaga ttattctgta
      121 attaactgca taatttgtca cgtgccatag ttttacattc cactacgtca tagttcttaa
      181 aatactaatc tcctgaaaat agaagtaggt gaagaaagtt taattatcag ttctaaaatg
      241 acaattgatc tttggaatat gttctgaaac taccgatcat tgaacagatg ctatttgaat
      301 gatatagaat tgtatatttg caatttctga aacgcgttct taaaggcaca cagattaatt
      361 caaaagggtc tggccgcaaa aaggtttatg gtggccgatt ttgagttttg tgtgtgattg
      421 ctttttcaca atcagtgttt tcaggattat gtgatgaact agatcttcaa gtttcgttac
      481 atttcatatg ttttcggaac tcacgaagta catattgggt attgtgctca aaaaattcag
      541 caatcagctt cgctccgctg actttagaac ccaaaaaaat agtatggcca aactgactgt
      601 gttacgatca tttcaatttt tcaatacata tttaagattt ctaagagtaa gaaggtcaaa
      661 aactgttctg gaatacatat atatttttca ggttacaatt agtcaaaaag tgcactgaaa
      721 tatacgtttt aatttcacga ataacccaat tagttcaatg tatttttggt caaccaacgt
      781 taaagtttgg cttccaacca attatcattt ctgatcaacc acaatgtttt ttctttatct
      841 gcaagttaat tttttatttt tatccagatg tttggcatat ttttcaattc ttcactagcg
      901 cccacttctt gcacttccgg cgccctgaat ctaatgcatc tgttgcaaga attgaaagac
      961 caatcaacac attgttttct tcacgagata ctgaagaaaa tgaataaaaa cagagaaaaa
     1021 gagccatgtg attagtgaca actgttgcta acagatacca tagcttggac ttggtacgtg
     1081 atggcaacgt atgggtcaac aaaaatgatt gcagaggggg tgcaaaacag tcaagtcgag
     1141 aaaatatgaa aaacagaaaa caaagaacag aaaaatgggt ttgagagtca gtataattta
     1201 taaaagaaaa attgtacata gaaattaacc atttttgtag aagaagttat ttttcaagca
     1261 tcgttaaaaa ttattcaaag caccttattt catatttaat tttaaacatg gttaaatgaa
     1321 caacacggtg cgcaatcagg aaaacttgaa atctgaaact gttgttgtga tcttcttcgc
     1381 aactgttcag atagcactag tgtaatgtta agagtgcgcg aatataatgg aatataatgg
     1441 atcacacctc ctgccatcag gtaaacgtct ctgttatcac atatttccaa ctattaaatt
     1501 tttacctttt acagttttac atttttttga aaaaagtaac tttttgtctt caaaatccct
     1561 gacgaaaata tcaaatattt taatcgagac tgcagaggaa ccgattgatg atttggaaaa
     1621 tccagcttta cctgtgtaag aactgaaaag tttcataacc ctagggtatt cccagttaca
     1681 ttccccactg gctaacaata gcacccagtt tttcatcacc tttcttcaaa tttctcggcg
     1741 atttgttaaa aacaaaattt gtgtcccttc tctgatatct ctatgtctct aaacacaagt
     1801 tcatcggaaa acgaaggagg gtaggtgttg gttgggctcc cgaagtgaaa atagaagagc
     1861 aagaatagaa tattagagag agagtgcaga gagggcggga tagctcccgg gattccgttt
     1921 tcttcttctt tatcttcaac gatgatgtgt gtgcgtgttg tatagattct gttgctcccc
     1981 cacaactcgc tccgaaggct caatacaatt caattgatat tggaggagag cctaccggag
     2041 tgggaggata agaagaaaca taagaagaag aagaagaaga agcatgcttc tggtttttga
     2101 tgctatgaaa acggcacaaa aagatgattg aggtcccttt tcaatacctt ctctcatctt
     2161 tcaaatccca ttgaaaccta aaacttctca ccacgcttta ccattgttct ccaaaaactt
     2221 atagcaatgt ctataacttt tttatctctg aaaagcagtg ttccattttt ctttttccta
     2281 ttttatttca attgtttctc acatttcgtt tggattcttt gcttgtcaac cagcttcttc
     2341 ttccactttt accgtctaat tttcagggca gggagccatc aaacccacga ccactagatc
     2401 catctagagg atccccggga ttggccaaag gacccaaagg tatgtttcga atgatactaa
     2461 cataacatag aacattttca ggaggaccct tggctagcgt cgacggtacc ggtaaaaatg
     2521 gacgacgatg tcggtgaacg aacaccggct ctcggtggtt catgtggacc atcagttcgt
     2581 gcacacagta gttccccaag acgtgttcca ttatttgaga gagcatctgc tagatggtgg
     2641 aatcctcaat ttcgatctgc aacactagaa gctcaatatt ggaagtgtag tttcagtcag
     2701 ttaagagatc gatttcgttc tggattaata tatattgccg ttgtaattgc cgcttggaca
     2761 ctttatttag ctttattcga tagaactttt attcagcatt ggatcgtttc actatgcctc
     2821 tgtgccatta ttttcgcgat gtttgccttc acggcgtgcg ccgctcaata ccaaaggttc
     2881 tacatgccaa cctcatttct atgcactttt ttgatatgcc ttgtcacgtt gctcatcttc
     2941 tccgcagaga atcaagctgc gtttatgact ccagttgcaa gtcttgccac aagttttcag
     3001 gtcgttctcc tgatctacac agtcattcca ctgccactct atttatgtat actcatcgga
     3061 ataatctact caatcctatt tgagattctc aacaagaaca aaattggtct tgaagaggcc
     3121 ggatacatta agcttgtcct tcacgctggt gtccatttac tcggtgtcca tttgttcatt
     3181 ttaactcaag tgcgacagag gaaaacgttt ctgaaagtgg gacaatcgat gttggcaaga
     3241 aaggatctag agttggagac acagtttaaa gatcatatga ttcagtctgt gatgcctaaa
     3301 aaggtagctg atgaattact gaaagatgca tcagaacttc gccgaccttc tgcttccaac
     3361 gactcaaact gccgaacttc aaatgctaca caagttgacc agccccttgc caagatggta
     3421 cctgaatacc gaaagttccg tccatttacg atgaatctca tgacaaatgt gtcgattctg
     3481 tttgcggata ttgctgggtt tactaaaatg tcatcaaata aatctgccga cgaacttgtc
     3541 aatctgctca atgatctttt tggaagattc gatactttgt gtcgattgag aggattagag
     3601 aagatttcaa ctttgggaga ttgttactat tgtgttgctg gatgtcctga accatgtgat
     3661 gatcatgcat gcagaacagt cgaaatgggt ctcgacatga ttgttgcaat ccgccaattt
     3721 gatattgatc gtggacaaga agtgaatatg cgtgtgggaa ttcatacagg aaaagtaatg
     3781 tgcggaatgg ttggaacaaa aagattcaaa ttcgatgtgt tctcaaacga tgtcacactg
     3841 gcaaatgaga tggaatcttc tggtgttgct ggaagagttc acgtttccga agcaactgca
     3901 aaacttttga aagggcttta tgaaattgaa gagggaccgg attatgatgg tccattgagg
     3961 atgcaagttc agggaactga acgtcgtgtt aaaccagaaa gcatgaagac tttctttatc
     4021 aaaggacgta ttaatgatgg tgtagaagaa gaagtgatgc aagttcagga agttgagagc
     4081 cttcacagtc agaaaagcag taaaaagtcg actctaaaac agaaatgggc ggaaaagttg
     4141 aaaatgaacc acacaaattc ttatccaatg cgagccgctg cacgtgaagg aggtggaagt
     4201 ttgagaataa aattggcaga gagaaataga agtacacagt tactgccaaa agaatcaaat
     4261 tcaatttgta taatggaaga taaccgaaag tcagcatcac ttcaagcatt ggcaacgaat
     4321 aattttaatg gaagtaatac agatacaaat aacacttata gtgaacgtgg agttgctgga
     4381 agtgttagta agaagtctgt agctggaagt gaaagtaata gtataaaagg aagtagaagc
     4441 agtggactac aattatctct acaagatgga aattctgatt taaattcagt tggaggtttg
     4501 gatactgcaa tcagtcacca tcacaatgca gcatctctca ctcgatttga tactgataac
     4561 aattttgatc agcgtttggc aatggtaatt ggacaaggag aaggaggatt tgataaagga
     4621 ttttggaatc atcatgatag tttgaataag tggactcttc gatttaatga aaaggacgtt
     4681 gaagaggaat atcgagctca ttttgtggat tcatcggaaa gatatacggc atcaaagaaa
     4741 ggtcatgttg aaagacataa agatttgatg gaacaaggtg gagaaaaaga tggaattact
     4801 ggatcaacgg tcaacaagta tagatattca ggagtcttca tcgatataat tgttgccacg
     4861 ttgatatttg taatatcggg tgcagttgca attatgtctg tccgtccatt cccattatca
     4921 ctcattgcat acttcccatt tgctgctgca attctcattc ttacaattgt ccttattggt
     4981 cttccacttc tcgcccgtaa aaaatcattc caatgtgcaa atcaatggat gccacgtcat
     5041 ctcatcggtc ttcttctgat ctttcttcca atcggtgtcg caatctgtat catgccattg
     5101 tgccaaagtg gagactgtgc aaatgtaatt ctgaactacc gtcttgcatt cagttatgtc
     5161 accattcttg ccatattcgc tcattgcaac ttctcccaat tagctgcctg gccaaaaact
     5221 actgctgcag tatttatcgg acttctccac attgctggag ttttctactg cgaattcaat
     5281 ttgaaacact tagttgaaga gcaagacacc tgcaacgtga cagcaatcat gattccacca
     5341 attcgcaaag gtctgaacta cacaattgca ttgaactcga catctgcaag aacattatct
     5401 caagattttg gatctccatt attcatttgg gagctgcttc tggatgtgat cctgtcgatt
     5461 gttctagttg cattccttaa ttatcagttc gaaaccgcat tccgtatgtc attctttgga
     5521 gatgttcaag ctcgtcggga cactgaaaga atgcaaatcg tgcgtgatca agccgattgg
     5581 ctcctgaata acgtgatccc agcccacgca gtagaaagtc ttaaaacgga taccaaatac
     5641 agtgaaaacc atgaaacagt tggtgtactg ttcgcttcaa tcactaattg gaatgatatg
     5701 tatgaagaga atttcgaagg gggcagagaa ttcttgagag ttctcaatga agttattggt
     5761 gactttgatg agcttctcga tcggccggac ttcacgcata ttgaaaaaat caaaacaatt
     5821 gggcctgcgt atatggcagc aagtggattg aatccagaac gaaaaaagaa tatgttgcat
     5881 ccaaaagagc atctatatca gatggttgat tttgcattgg cagttcaaca tgtactcagt
     5941 gttttcaacg aggatttgct caattttgat tttgtttgca agttaggact taatattgga
     6001 cccgtcactg ctggtgtcat tggaacaaca aagttgtact acgatatctg gggtgacact
     6061 gtaaacattg ctagccgaat gtacagcacc ggagttttaa atcgcattca agtatcacaa
     6121 cacacacgag aatatctcct ggatcggtat gaattcgagt tccgcgatca tattgaagtc
     6181 aagggaattg atggaggaat ggatacttat cttttggttg gtcgaaaagg tgatggaata
     6241 cctccatcaa tcaaggataa ccaagaagac gaattttaaa tctcgaggag ctcccgagat
     6301 cttcagcgct cagcattcgt agaattgtaa gtttaaactt tctcatacta actaacgcgc
     6361 tctatttaaa ttttcagaat tccaactgag cgccggtcgc taccattacc aacttgtctg
     6421 gtgtcaaaaa taataggggc cgctgtcatc agagtaagtt taaactgagt tctactaact
     6481 aacgagtaat atttaaattt tcagcatctc gcgcccgtgc ctctgacttc taagtccaat
     6541 tactcttcaa catccctaca tgctctttct ccctgtgctc ccacccccta tttttgttat
     6601 tatcaaaaaa cttctcttaa tttctttgtt ttttagcttc ttttaagtca cctctaacaa
     6661 tgaaattgtg tagattcaaa aatagaatta attcgtaata aaaagtcgaa aaaaattgtg
     6721 ctccctcccc ccattaataa taattctatc ccaaaatcta cacaatgttc tgtgtacact
     6781 tcttatgttt tttacttctg ataaattttt ttgaaacatc atagaaaaaa ccgcacacaa
     6841 aataccttat catatgttac gtttcagttt atgaccgcaa tttttatttc ttcgcacgtc
     6901 tgggcctctc atgacgtcaa atcatgctca tcgtgaaaaa gttttggagt atttttggaa
     6961 tttttcaatc aagtgaaagt ttatgaaatt aattttcctg cttttgcttt ttggggtttc
     7021 ccctattgtt tgtcaagatt tcgaggacgg cgtttttctt gctaaaatca caagtattga
     7081 tgagcacgat gcaagaaaga tcggaagaag gtttgggttt gaggctcagt ggaaggtgag
     7141 tagaagttga taatttgaaa gtggagtagt gtctatgggg tttttgcctt aaatgacaga
     7201 atacattccc aatataccaa acataactgt ttcctactag tcggccgtac gggccctttc
     7261 gtctcgcgcg tttcggtgat gacggtgaaa acctctgaca catgcagctc ccggagacgg
     7321 tcacagcttg tctgtaagcg gatgccggga gcagacaagc ccgtcagggc gcgtcagcgg
     7381 gtgttggcgg gtgtcggggc tggcttaact atgcggcatc agagcagatt gtactgagag
     7441 tgcaccatat gcggtgtgaa ataccgcaca gatgcgtaag gagaaaatac cgcatcaggc
     7501 ggccttaagg gcctcgtgat acgcctattt ttataggtta atgtcatgat aataatggtt
     7561 tcttagacgt caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt
     7621 ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa
     7681 taatattgaa aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt
     7741 tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat
     7801 gctgaagatc agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag
     7861 atccttgaga gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg
     7921 ctatgtggcg cggtattatc ccgtattgac gccgggcaag agcaactcgg tcgccgcata
     7981 cactattctc agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat
     8041 ggcatgacag taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc
     8101 aacttacttc tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg
     8161 ggggatcatg taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac
     8221 gacgagcgtg acaccacgat gcctgtagca atggcaacaa cgttgcgcaa actattaact
     8281 ggcgaactac ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa
     8341 gttgcaggac cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct
     8401 ggagccggtg agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc
     8461 tcccgtatcg tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga
     8521 cagatcgctg agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac
     8581 tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag
     8641 atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg
     8701 tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc
     8761 tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag
     8821 ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtt
     8881 cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac
     8941 ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc
     9001 gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt
     9061 tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt
     9121 gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc
     9181 ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt
     9241 tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca
     9301 ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt
     9361 tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt
     9421 attaccgcct ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag
     9481 tcagtgagcg aggaagcgga agagcgccca atacgcaaac cgcctctccc cgcgcgttgg
     9541 ccgattcatt aatgcagctg gcacgacagg tttcccgact ggaaagcggg cagtgagcgc
     9601 aacgcaatta atgtgagtta gctcactcat taggcacccc aggctttaca ctttatgctt
     9661 ccggctcgta tgttgtgtgg aattgtgagc ggataacaat ttcacacagg aaacagctat
     9721 gaccatgatt acgccaagct gtaagtttaa acatgatctt actaactaac tattctcatt
     9781 taaattttca gagcttaaaa atggctgaaa tcactcacaa cgatggatac gctaacaact
     9841 tggaaa
//
