LOCUS       sequence-21256-e         743 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 743)
  AUTHORS   Fiordalisi JJ, Johnson RL 2nd, Ulku AS, Der CJ, Cox AD
  TITLE     Mammalian expression vectors for Ras family proteins: generation and
            use of expression constructs to analyze Ras family function.
  JOURNAL   Methods Enzymol. 2001;332:3-36. doi: 10.1016/s0076-6879(01)32189-4.
  PUBMED    11305105
REFERENCE   2  (bases 1 to 743)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Methods 
            Enzymol."; date: "2001"; volume: "332:3-36. doi"; pages: " 
            10.1016/s0076-6879(01)32189-4"
COMMENT     Sequence Label: pCGN N-Ras 189L
FEATURES             Location/Qualifiers
     source          1..743
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     5'UTR           49..88
                     /label=HSV TK 5'-UTR
                     /note="5' untranslated region from the herpes simplex virus
                     thymidine kinase gene"
     CDS             118..144
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             124..150
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /note="This is a variant of the standard HA tag, which has 
                     sequence YPYDVPDYA. Some anti-HA antibodies may not 
                     recognize this variant."
                     /translation="YDVPDYASL"
ORIGIN
        1 ctgttttgac ctccntagaa ganaccggga ccgatccagc ctccggggga tcttggtggc
       61 gtgaaactcc cgcacctctt cggccagcgc cttgtagaag cgcgtatggc ttctagctat
      121 ccttatgacg tgcctgacta tgccagcctg ggaggacctt ctagcggatc caccatgact
      181 gagtacaaac tggtggtggt tggagcaggt ggtgttggga aaagcgcact gacaatccag
      241 ctaatccaga accactttgt agatgaatat gatcccacca tagaggattc ttacagaaaa
      301 caagtggtta tagatggtga aacctgtttg ttggacatac tggatacagc tggacaagaa
      361 gagtacagtg ccatgagaga ccaatacatg aggacaggcg aaggcttcct ctgtgtattt
      421 gccatcaata atagcaagtc atttgcggat attaacctct acagggagca gattaagcga
      481 gtaaaagact cggatgatgt acctatggtg ctagtgggaa acaagtgtga tttgccaaca
      541 aggacagttg atacaaaaca agcccacgaa ctggccaaga gttacgggat tccattcatt
      601 gaaacctcag ccaagaccag acagggtgtt gaagatgctt tttacacact ggtaagagna
      661 atacgccagt accgaatgaa aaaactcnan nagcagtgat gatgggactc nnnntgtatg
      721 ggattgccat gtgtggtgtt gta
//
