LOCUS       sequence-212562-        6353 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Vector for expressing proteins in a subset of 9 DA/ DB cholingergic 
            motor neurons in the C. elegans ventral nerve cord.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6353)
  AUTHORS   Edwards SL, Charlie NK, Richmond JE, Hegermann J, Eimer S, Miller KG
  TITLE     Impaired dense core vesicle maturation in Caenorhabditis elegans 
            mutants lacking Rab2.
  JOURNAL   J Cell Biol. 2009 Sep 21;186(6):881-95. doi: 10.1083/jcb.200902095.
  PUBMED    19797080
REFERENCE   2  (bases 1 to 6353)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1083/jcb"; 
            journalName: "J Cell Biol"; date: "2009-09-21- 21"; volume: "186"; 
            issue: "6"; pages: "881-95"
COMMENT     Sequence Label: KG#244
FEATURES             Location/Qualifiers
     source          1..6353
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(3820..3842)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     3942..3961
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(4039..4057)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        4125..4229
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4230..4298,4299..5090)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4448..4467)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5261..5849
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5750..5769
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6003..6020
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    6137..6158
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(6173..6178,6179..6196,6197..6203)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6211..6227
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     6216..6238
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     6235..6251
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     6235..6251
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 gatacgctaa caacttggaa atgaaataag cttgggctgc agcccgggaa acatgatatc
       61 gacggacata tttttgattg attatcataa ttttcgtggg aatcttcgga acatgctctt
      121 tgcggtttat aagtggagat gcgccaacgg gaaacttgtt ttaaaactat tcctatggtg
      181 ctaacatcac cacatgaaaa cttttcaaaa cttttttggg ttttttattt ttttattatt
      241 attcgtatct aaacattgta ggcgttgtga tgttgcccta aatttcttta aaaagagcga
      301 acattttcaa aaatttgcta tttaaatcga atgaaaattt ccattcaaat atttgaaaaa
      361 aattatgaaa aaattccttt tgttgcatct tcatcttcta tgaaagttac aattttagtt
      421 ggatcagaaa tatgagatat caaaaaaaaa ttaagatttt tttgtttgtt tttttgaagc
      481 ctaaaatatt ttgatatcat ttatctctta tgtcttttgt tttatcgaaa cgtttacaac
      541 acatcaacta tgcgtcaata aattttttag gtagttaaac aaatgttgaa aaaaaaagca
      601 aaagcaacca agagctgtta aaatagagaa aatgcagtta ttttttatat tcaagaaaag
      661 ttatcacaga agttccgtaa tgagtttttt ccttatcaac atgttcccat caacttctta
      721 gtttctcctc attcccattg ttcaacataa ttatcacctc tgaccttatt ctgctctttt
      781 taagcacagg tgttaatatg ccgtccatca tatttgccct ccgtctgccg ctttccttat
      841 ctgaccttta catcgccttt ttgtatatgt acataataat tgtatcatca tcaacctccg
      901 ccataaacat gcatgtcttt ttacctcttt tggcatgtac cgttcttctt tgtattttta
      961 ttatgtcttc cttccatctt ccctcttttt tacggatttt cggattgcgg aagaagaaga
     1021 tggtcgataa ttaccgtaat gaggtaattt gaaacatatt ggacagaaga aatacgcgcg
     1081 gatgctttcg gtggcaaggt taattcgttg tctttgtttc tggaatgtcc ttcataaaaa
     1141 acacggatta ccacctgttt ttaacgtgga ttaagatttt tggaaacaat ttactctgcc
     1201 taattttttt gtacatttaa aaatgtagac ttaccgaatt caccgagctt gaccagtttt
     1261 aaaggttacc gttcacacta aaaattgttg aaaaaatttt cgactaaacc taatgatttt
     1321 attttaaaaa ataaatgaaa tagcatacaa atttggattt ttttcaattt ttttcaagaa
     1381 aatcatgaaa catttagaaa actcgagtaa caagctttcc gttcattctg taatttttaa
     1441 gttccaaatt taaaaaaatg cattttgatt tactgggatt gccgtgctcg taattatcag
     1501 caaaacattt gccgaattag ccgagctcga caatctattt tttcgcaaac ttttgccatg
     1561 tttcgtaaaa caattttcca ttttatggta gacatttttc atcaatgaat tcacaaaatt
     1621 taaaatcccc actgaaagta ccaaaatctt cctgaaaaat ccggcaaaaa atccatctca
     1681 ttatcatgtc acatatacat gttctattgg cactatgttt gggatgtcct ccaattaata
     1741 cttctgtgtc aatctcatcg gcgcccggcg gccactacca aatgcaaaac tttgtttgta
     1801 aaagaagaag ccaataattt tctcgttatt catttttatt actctttttg cgtttcattc
     1861 ttctttgttc gaattgatga acgcacgttg cttctctctg gttttcctgc cgttccttat
     1921 attgccaagg aaaccaatgc ggattttcgg aagattttct tcatgtcctt tagaaaatat
     1981 caccactttt cttctttttc catttcaaat ttcaatttcc atacaactca tcttctatta
     2041 tagcttttca ggtgttcgac tgtttgaaga taagaatcaa ttaaaatttg gttcactgag
     2101 catgtcatcc acttttcctt ttctttgttc taaaattcac caaactgaat tttccttaac
     2161 cggaaattta catgaaaata agaaacatgg attacatcct ttacatatca tttttgtttt
     2221 tttatgtgta ttccatgttc ccaaaagttt gatgcaattt ttgattcttt ttcaattttc
     2281 caaattcgtg tctcgcaggg aacgattcac agtccaacat tcccaaattg cgaaatttta
     2341 gaatatatat tcccaagcgt cgaatatctc aaaaatttgt cgttttctca gtttgacatg
     2401 tcacctttgc cggttcctct attcgtctat tagctctcca gagagcaatg tcatgtcaca
     2461 cgaagaattc agggacatgt ttcgtttttt tttgtttgca tgtggtcacc gatatgggaa
     2521 cttgaacttt ccaatttttg cttgcttttt cttttccgga aacttaatac tatttgagaa
     2581 actccgcatc ttatttgttt gatcccaaat tctgaaattc ctctttgaat ttgatctcaa
     2641 gcttcactat aaaaaattgt ttcaggaaaa ttggaagagc aagcggatcc ccgggattgg
     2701 ccaaaggacc caaaggtatg tttcgaatga tactaacata acatagaaca ttttcaggag
     2761 gacccttggc tagcgtcgac ggtaccggtc agatatcact cgaggacttg gcggccgcaa
     2821 tcggagatct tcagcgctca gcattcgtag aattgtaagt ttaaactttc tcatactaac
     2881 taacgcgctc tatttaaatt ttcagaattc caactgagcg ccggtcgcta ccattaccaa
     2941 cttgtctggt gtcaaaaata ataggggccg ctgtcatcag agtaagttta aactgagttc
     3001 tactaactaa cgagtaatat ttaaattttc agcatctcgc gcccgtgcct ctgacttcta
     3061 agtccaatta ctcttcaaca tccctacatg ctctttctcc ctgtgctccc accccctatt
     3121 tttgttatta tcaaaaaact tctcttaatt tctttgtttt ttagcttctt ttaagtcacc
     3181 tctaacaatg aaattgtgta gattcaaaaa tagaattaat tcgtaataaa aagtcgaaaa
     3241 aaattgtgct ccctcccccc attaataata attctatccc aaaatctaca caatgttctg
     3301 tgtacacttc ttatgttttt tacttctgat aaattttttt gaaacatcat agaaaaaacc
     3361 gcacacaaaa taccttatca tatgttacgt ttcagtttat gaccgcaatt tttatttctt
     3421 cgcacgtctg ggcctctcat gacgtcaaat catgctcatc gtgaaaaagt tttggagtat
     3481 ttttggaatt tttcaatcaa gtgaaagttt atgaaattaa ttttcctgct tttgcttttt
     3541 ggggtttccc ctattgtttg tcaagatttc gaggacggcg tttttcttgc taaaatcaca
     3601 agtattgatg agcacgatgc aagaaagatc ggaagaaggt ttgggtttga ggctcagtgg
     3661 aaggtgagta gaagttgata atttgaaagt ggagtagtgt ctatggggtt tttgccttaa
     3721 atgacagaat acattcccaa tataccaaac ataactgttt cctactagtc ggccgtacgg
     3781 gccctttcgt ctcgcgcgtt tcggtgatga cggtgaaaac ctctgacaca tgcagctccc
     3841 ggagacggtc acagcttgtc tgtaagcgga tgccgggagc agacaagccc gtcagggcgc
     3901 gtcagcgggt gttggcgggt gtcggggctg gcttaactat gcggcatcag agcagattgt
     3961 actgagagtg caccatatgc ggtgtgaaat accgcacaga tgcgtaagga gaaaataccg
     4021 catcaggcgg ccttaagggc ctcgtgatac gcctattttt ataggttaat gtcatgataa
     4081 taatggtttc ttagacgtca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt
     4141 gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa
     4201 tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta
     4261 ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag
     4321 taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca
     4381 gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta
     4441 aagttctgct atgtggcgcg gtattatccc gtattgacgc cgggcaagag caactcggtc
     4501 gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc
     4561 ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca
     4621 ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc
     4681 acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca
     4741 taccaaacga cgagcgtgac accacgatgc ctgtagcaat ggcaacaacg ttgcgcaaac
     4801 tattaactgg cgaactactt actctagctt cccggcaaca attaatagac tggatggagg
     4861 cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg
     4921 ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg
     4981 gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac
     5041 gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc
     5101 aagtttactc atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct
     5161 aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc
     5221 actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc
     5281 gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg
     5341 atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa
     5401 atactgttct tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc
     5461 ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt
     5521 gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa
     5581 cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc
     5641 tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc
     5701 cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct
     5761 ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat
     5821 gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc
     5881 tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg
     5941 ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc
     6001 gcagcgagtc agtgagcgag gaagcggaag agcgcccaat acgcaaaccg cctctccccg
     6061 cgcgttggcc gattcattaa tgcagctggc acgacaggtt tcccgactgg aaagcgggca
     6121 gtgagcgcaa cgcaattaat gtgagttagc tcactcatta ggcaccccag gctttacact
     6181 ttatgcttcc ggctcgtatg ttgtgtggaa ttgtgagcgg ataacaattt cacacaggaa
     6241 acagctatga ccatgattac gccaagctgt aagtttaaac atgatcttac taactaacta
     6301 ttctcattta aattttcaga gcttaaaaat ggctgaaatc actcacaacg atg
//
