LOCUS       sequence-212563-        4923 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses proteins pan-neuronally in C. elegans (entire nervous 
            system).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4923)
  AUTHORS   Schade MA, Reynolds NK, Dollins CM, Miller KG
  TITLE     Mutations that rescue the paralysis of Caenorhabditis elegans ric-8 
            (synembryn) mutants activate the G alpha(s) pathway and define a 
            third major branch of the synaptic signaling network.
  JOURNAL   Genetics. 2005 Feb;169(2):631-49. doi: 10.1534/genetics.104.032334. 
            Epub 2004 Oct 16.
  PUBMED    15489510
REFERENCE   2  (bases 1 to 4923)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1534/genetics.104.032334"; journalName: "Genetics"; date: 
            "2005-02"; volume: "169"; issue: "2"; pages: "631-49"
COMMENT     Sequence Label: KG#470
FEATURES             Location/Qualifiers
     source          1..4923
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(2438..2460)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     2560..2579
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(2657..2675)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        2743..2847
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(2848..2916,2917..3708)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(3066..3085)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      3879..4467
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4368..4387
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     4621..4638
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    4755..4776
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(4791..4796,4797..4814,4815..4821)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4829..4845
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     4834..4856
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     4853..4869
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     4853..4869
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 atttaaattt tcagagctta aaaatggctg aaatcactca caacgatgga tacgctaaca
       61 acttggaaat gaaataagct tgggctgcag gatcttcaga tgggagcagt ggactgtcta
      121 tttgatattc ttaaaatgta ctctataaaa gatgaaactg taatgaccaa ttccctacat
      181 aaatattttt aactaattgg tcggatattt ggggatcaga gaagtacttg tttttgcgat
      241 attaataatg atatattttt ctagtaaaaa aatgttaagc gatttgtgaa taattaagtt
      301 ggcttgaaaa agattttttg atagctatgt attttgttaa tttaaatcag attttaaaac
      361 tttattattg actatttata tatattatat attatatatt ttcatggttt aatcaaaatc
      421 tattaaatta tttctaaaat ctctaaatat cgaattctct aaaaagcttc atcaacaaaa
      481 gcatttcaat tatctaacac aatgagaata attcaagaac tgaaatagaa agaaatctag
      541 aaaaaaaaga gagtgaggtg ccctattaaa tcggtgctct cctctctaac agaagcgcct
      601 acacgcaccc aagagagctc tttttctttc ttttttcgga gcacacatac acgcatacac
      661 tcctcagaag tgtatagaga aaatgggaga agaagcgaaa gaagacgaag aagcggttgt
      721 gatgtatgca tcgggcgttt ctatctctct ccgtgagcaa cgagctagtc aacccatcga
      781 tccatttttg tgagcacaca cagagagaga ctcaaacaca ctcacagctt cacactgagc
      841 tccaagagct ctgtagagag aagccagagt atgctttcct cgattttcct aacgtcttct
      901 tcttctgctt ttttcttttt cccaacgagt tgtagtccac taacttcaat ttttcctcca
      961 gagaaagagt tttagagaga cagctgataa atacactaga gaaataaaaa gaatgataaa
     1021 aatttgctat tttaatcagt tccctctcgt ttctctaagt tttgttgttg ggttgggaaa
     1081 ttaaaattgg aaaatggaaa taacgttttt ttctgtctaa tcttgaattc tccagacatt
     1141 tcttatttcc ttttttgttg caagtttttc ttccttctct aatttttaat caaagatatc
     1201 tcgctatgaa ctttgaattt ttgatatttt gattctataa atacataaag taatctaaat
     1261 gctcttttaa aataaatcta cagtagccct attttcaggg atccccggga ttggccaaag
     1321 gacccaaagg tatgtttcga atgatactaa cataacatag aacattttca ggaggaccct
     1381 tggctagcag ctctcctgca gggttcgacg gtaccggtca gatatcactc gaggagctcc
     1441 cgagatcttc agcgctcagc attcgtagaa ttgtaagttt aaactttctc atactaacta
     1501 acgcgctcta tttaaatttt cagaattcca actgagcgcc ggtcgctacc attaccaact
     1561 tgtctggtgt caaaaataat aggggccgct gtcatcagag taagtttaaa ctgagttcta
     1621 ctaactaacg agtaatattt aaattttcag catctcgcgc ccgtgcctct gacttctaag
     1681 tccaattact cttcaacatc cctacatgct ctttctccct gtgctcccac cccctatttt
     1741 tgttattatc aaaaaacttc tcttaatttc tttgtttttt agcttctttt aagtcacctc
     1801 taacaatgaa attgtgtaga ttcaaaaata gaattaattc gtaataaaaa gtcgaaaaaa
     1861 attgtgctcc ctccccccat taataataat tctatcccaa aatctacaca atgttctgtg
     1921 tacacttctt atgtttttta cttctgataa atttttttga aacatcatag aaaaaaccgc
     1981 acacaaaata ccttatcata tgttacgttt cagtttatga ccgcaatttt tatttcttcg
     2041 cacgtctggg cctctcatga cgtcaaatca tgctcatcgt gaaaaagttt tggagtattt
     2101 ttggaatttt tcaatcaagt gaaagtttat gaaattaatt ttcctgcttt tgctttttgg
     2161 ggtttcccct attgtttgtc aagatttcga ggacggcgtt tttcttgcta aaatcacaag
     2221 tattgatgag cacgatgcaa gaaagatcgg aagaaggttt gggtttgagg ctcagtggaa
     2281 ggtgagtaga agttgataat ttgaaagtgg agtagtgtct atggggtttt tgccttaaat
     2341 gacagaatac attcccaata taccaaacat aactgtttcc tactagtcgg ccgtacgggc
     2401 cctttcgtct cgcgcgtttc ggtgatgacg gtgaaaacct ctgacacatg cagctcccgg
     2461 agacggtcac agcttgtctg taagcggatg ccgggagcag acaagcccgt cagggcgcgt
     2521 cagcgggtgt tggcgggtgt cggggctggc ttaactatgc ggcatcagag cagattgtac
     2581 tgagagtgca ccatatgcgg tgtgaaatac cgcacagatg cgtaaggaga aaataccgca
     2641 tcaggcggcc ttaagggcct cgtgatacgc ctatttttat aggttaatgt catgataata
     2701 atggtttctt agacgtcagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt
     2761 ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg
     2821 cttcaataat attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt
     2881 cccttttttg cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta
     2941 aaagatgctg aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc
     3001 ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa
     3061 gttctgctat gtggcgcggt attatcccgt attgacgccg ggcaagagca actcggtcgc
     3121 cgcatacact attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt
     3181 acggatggca tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact
     3241 gcggccaact tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac
     3301 aacatggggg atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata
     3361 ccaaacgacg agcgtgacac cacgatgcct gtagcaatgg caacaacgtt gcgcaaacta
     3421 ttaactggcg aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg
     3481 gataaagttg caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat
     3541 aaatctggag ccggtgagcg tgggtctcgc ggtatcattg cagcactggg gccagatggt
     3601 aagccctccc gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga
     3661 aatagacaga tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa
     3721 gtttactcat atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag
     3781 gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac
     3841 tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc
     3901 gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat
     3961 caagagctac caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat
     4021 actgttcttc tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct
     4081 acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt
     4141 cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg
     4201 gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta
     4261 cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg
     4321 gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg
     4381 tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc
     4441 tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg
     4501 gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat
     4561 aaccgtatta ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc
     4621 agcgagtcag tgagcgagga agcggaagag cgcccaatac gcaaaccgcc tctccccgcg
     4681 cgttggccga ttcattaatg cagctggcac gacaggtttc ccgactggaa agcgggcagt
     4741 gagcgcaacg caattaatgt gagttagctc actcattagg caccccaggc tttacacttt
     4801 atgcttccgg ctcgtatgtt gtgtggaatt gtgagcggat aacaatttca cacaggaaac
     4861 agctatgacc atgattacgc caagctgtaa gtttaaacat gatcttacta actaactatt
     4921 ctc
//
