LOCUS       sequence-212564-        4116 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Vector for expressing proteins in most or all tissues, including 
            nervous system, under control of the inducible heat shock promoter 
            in C. elegans.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4116)
  AUTHORS   Reynolds NK, Schade MA, Miller KG
  TITLE     Convergent, RIC-8-dependent Galpha signaling pathways in the 
            Caenorhabditis elegans synaptic signaling network.
  JOURNAL   Genetics. 2005 Feb;169(2):651-70. doi: 10.1534/genetics.104.031286. 
            Epub 2004 Oct 16.
  PUBMED    15489511
REFERENCE   2  (bases 1 to 4116)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1534/genetics.104.031286"; journalName: "Genetics"; date: 
            "2005-02"; volume: "169"; issue: "2"; pages: "651-70"
COMMENT     Sequence Label: KG#45
FEATURES             Location/Qualifiers
     source          1..4116
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    83..104
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(119..124,125..142,143..149)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    157..173
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     162..184
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     181..197
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     181..197
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1882..1904)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     2004..2023
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(2101..2119)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        2187..2291
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(2292..2360,2361..3152)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(2510..2529)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      3323..3911
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3812..3831
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     4065..4082
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 tccccgcgcg ttggccgatt cattaatgca gctggcacga caggtttccc gactggaaag
       61 cgggcagtga gcgcaacgca attaatgtga gttagctcac tcattaggca ccccaggctt
      121 tacactttat gcttccggct cgtatgttgt gtggaattgt gagcggataa caatttcaca
      181 caggaaacag ctatgaccat gattacgcca agctgtaagt ttaaacatga tcttactaac
      241 taactattct catttaaatt ttcagagctt aaaaatggct gaaatcactc acaacgatgg
      301 atacgctaac aacttggaaa tgaaataagc ttgcatgcct gcaggtcgac tctagaggat
      361 caagagcatt tgaatcagaa tatggagaac ggagcatgag cattttcgaa gttttttaga
      421 tgcactagaa caaagcgtgt tggcttcctc tgagcccgct ttccttatat acccgcattc
      481 tgcagcctta cagaatgttc tagaaggtcc tagatgcatt cgtttgaaaa tactcccggt
      541 gggtgcaaag agacgcagac ggaaaatgta tctgggtctc tttaatgtgt acactacttt
      601 tccatgtacc gaatgtgagt cgccctcctt ttgcaacaag cagctcgaat gttctagaaa
      661 aaggtggaaa atagtataaa taccgttgaa aataaatacc gaacaacatt tgctctaatt
      721 gtgaaattag aaatcttcaa actataatca tctcactgga tccccgggat tggccaaagg
      781 acccaaaggt atgtttcgaa tgatactaac ataacataga acattttcag gaggaccctt
      841 ggctagcgtc gacggtaccg gtcagatatc actcgaggag ctcccgagat cttcagcgct
      901 cagcattcgt agaattgtaa gtttaaactt tctcatacta actaacgcgc tctatttaaa
      961 ttttcagaat tccaactgag cgccggtcgc taccattacc aacttgtctg gtgtcaaaaa
     1021 taataggggc cgctgtcatc agagtaagtt taaactgagt tctactaact aacgagtaat
     1081 atttaaattt tcagcatctc gcgcccgtgc ctctgacttc taagtccaat tactcttcaa
     1141 catccctaca tgctctttct ccctgtgctc ccacccccta tttttgttat tatcaaaaaa
     1201 cttctcttaa tttctttgtt ttttagcttc ttttaagtca cctctaacaa tgaaattgtg
     1261 tagattcaaa aatagaatta attcgtaata aaaagtcgaa aaaaattgtg ctccctcccc
     1321 ccattaataa taattctatc ccaaaatcta cacaatgttc tgtgtacact tcttatgttt
     1381 tttacttctg ataaattttt ttgaaacatc atagaaaaaa ccgcacacaa aataccttat
     1441 catatgttac gtttcagttt atgaccgcaa tttttatttc ttcgcacgtc tgggcctctc
     1501 atgacgtcaa atcatgctca tcgtgaaaaa gttttggagt atttttggaa tttttcaatc
     1561 aagtgaaagt ttatgaaatt aattttcctg cttttgcttt ttggggtttc ccctattgtt
     1621 tgtcaagatt tcgaggacgg cgtttttctt gctaaaatca caagtattga tgagcacgat
     1681 gcaagaaaga tcggaagaag gtttgggttt gaggctcagt ggaaggtgag tagaagttga
     1741 taatttgaaa gtggagtagt gtctatgggg tttttgcctt aaatgacaga atacattccc
     1801 aatataccaa acataactgt ttcctactag tcggccgtac gggccctttc gtctcgcgcg
     1861 tttcggtgat gacggtgaaa acctctgaca catgcagctc ccggagacgg tcacagcttg
     1921 tctgtaagcg gatgccggga gcagacaagc ccgtcagggc gcgtcagcgg gtgttggcgg
     1981 gtgtcggggc tggcttaact atgcggcatc agagcagatt gtactgagag tgcaccatat
     2041 gcggtgtgaa ataccgcaca gatgcgtaag gagaaaatac cgcatcaggc ggccttaagg
     2101 gcctcgtgat acgcctattt ttataggtta atgtcatgat aataatggtt tcttagacgt
     2161 caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac
     2221 attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa
     2281 aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat
     2341 tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc
     2401 agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga
     2461 gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg
     2521 cggtattatc ccgtattgac gccgggcaag agcaactcgg tcgccgcata cactattctc
     2581 agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag
     2641 taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc
     2701 tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg
     2761 taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg
     2821 acaccacgat gcctgtagca atggcaacaa cgttgcgcaa actattaact ggcgaactac
     2881 ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac
     2941 cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg
     3001 agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg
     3061 tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg
     3121 agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac
     3181 tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg
     3241 ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg
     3301 tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc
     3361 aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc
     3421 tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtt cttctagtgt
     3481 agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc
     3541 taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact
     3601 caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac
     3661 agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag
     3721 aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg
     3781 gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg
     3841 tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga
     3901 gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt
     3961 ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct
     4021 ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg
     4081 aggaagcgga agagcgccca atacgcaaac cgcctc
//
