LOCUS       sequence-212565-        4908 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses proteins pan-neuronally in C. elegans (entire nervous 
            system).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4908)
  AUTHORS   Schade MA, Reynolds NK, Dollins CM, Miller KG
  TITLE     Mutations that rescue the paralysis of Caenorhabditis elegans ric-8 
            (synembryn) mutants activate the G alpha(s) pathway and define a 
            third major branch of the synaptic signaling network.
  JOURNAL   Genetics. 2005 Feb;169(2):631-49. doi: 10.1534/genetics.104.032334. 
            Epub 2004 Oct 16.
  PUBMED    15489510
REFERENCE   2  (bases 1 to 4908)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1534/genetics.104.032334"; journalName: "Genetics"; date: 
            "2005-02"; volume: "169"; issue: "2"; pages: "631-49"
COMMENT     Sequence Label: KG#59
FEATURES             Location/Qualifiers
     source          1..4908
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(2356..2378)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     2478..2497
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(2575..2593)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        2661..2765
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(2766..2834,2835..3626)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(2984..3003)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      3797..4385
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4286..4305
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     4539..4556
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    4673..4694
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(4709..4714,4715..4732,4733..4739)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4747..4763
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     4752..4774
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     4771..4787
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     4771..4787
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 aatgaaataa gcttgggctg caggatcttc agatgggagc agtggactgt ctatttgata
       61 ttcttaaaat gtactctata aaagatgaaa ctgtaatgac caattcccta cataaatatt
      121 tttaactaat tggtcggata tttggggatc agagaagtac ttgtttttgc gatattaata
      181 atgatatatt tttctagtaa aaaaatgtta agcgatttgt gaataattaa gttggcttga
      241 aaaagatttt ttgatagcta tgtattttgt taatttaaat cagattttaa aactttatta
      301 ttgactattt atatatatta tatattatat attttcatgg tttaatcaaa atctattaaa
      361 ttatttctaa aatctctaaa tatcgaattc tctaaaaagc ttcatcaaca aaagcatttc
      421 aattatctaa cacaatgaga ataattcaag aactgaaata gaaagaaatc tagaaaaaaa
      481 agagagtgag gtgccctatt aaatcggtgc tctcctctct aacagaagcg cctacacgca
      541 cccaagagag ctctttttct ttcttttttc ggagcacaca tacacgcata cactcctcag
      601 aagtgtatag agaaaatggg agaagaagcg aaagaagacg aagaagcggt tgtgatgtat
      661 gcatcgggcg tttctatctc tctccgtgag caacgagcta gtcaacccat cgatccattt
      721 ttgtgagcac acacagagag agactcaaac acactcacag cttcacactg agctccaaga
      781 gctctgtaga gagaagccag agtatgcttt cctcgatttt cctaacgtct tcttcttctg
      841 cttttttctt tttcccaacg agttgtagtc cactaacttc aatttttcct ccagagaaag
      901 agttttagag agacagctga taaatacact agagaaataa aaagaatgat aaaaatttgc
      961 tattttaatc agttccctct cgtttctcta agttttgttg ttgggttggg aaattaaaat
     1021 tggaaaatgg aaataacgtt tttttctgtc taatcttgaa ttctccagac atttcttatt
     1081 tccttttttg ttgcaagttt ttcttccttc tctaattttt aatcaaagat atctcgctat
     1141 gaactttgaa tttttgatat tttgattcta taaatacata aagtaatcta aatgctcttt
     1201 taaaataaat ctacagtagc cctattttca gggatccccg ggattggcca aaggacccaa
     1261 aggtatgttt cgaatgatac taacataaca tagaacattt tcaggaggac ccttggctag
     1321 cgtcgacggt accggtcaga tatcactcga ggagctcccg agatcttcag cgctcagcat
     1381 tcgtagaatt gtaagtttaa actttctcat actaactaac gcgctctatt taaattttca
     1441 gaattccaac tgagcgccgg tcgctaccat taccaacttg tctggtgtca aaaataatag
     1501 gggccgctgt catcagagta agtttaaact gagttctact aactaacgag taatatttaa
     1561 attttcagca tctcgcgccc gtgcctctga cttctaagtc caattactct tcaacatccc
     1621 tacatgctct ttctccctgt gctcccaccc cctatttttg ttattatcaa aaaacttctc
     1681 ttaatttctt tgttttttag cttcttttaa gtcacctcta acaatgaaat tgtgtagatt
     1741 caaaaataga attaattcgt aataaaaagt cgaaaaaaat tgtgctccct ccccccatta
     1801 ataataattc tatcccaaaa tctacacaat gttctgtgta cacttcttat gttttttact
     1861 tctgataaat ttttttgaaa catcatagaa aaaaccgcac acaaaatacc ttatcatatg
     1921 ttacgtttca gtttatgacc gcaattttta tttcttcgca cgtctgggcc tctcatgacg
     1981 tcaaatcatg ctcatcgtga aaaagttttg gagtattttt ggaatttttc aatcaagtga
     2041 aagtttatga aattaatttt cctgcttttg ctttttgggg tttcccctat tgtttgtcaa
     2101 gatttcgagg acggcgtttt tcttgctaaa atcacaagta ttgatgagca cgatgcaaga
     2161 aagatcggaa gaaggtttgg gtttgaggct cagtggaagg tgagtagaag ttgataattt
     2221 gaaagtggag tagtgtctat ggggtttttg ccttaaatga cagaatacat tcccaatata
     2281 ccaaacataa ctgtttccta ctagtcggcc gtacgggccc tttcgtctcg cgcgtttcgg
     2341 tgatgacggt gaaaacctct gacacatgca gctcccggag acggtcacag cttgtctgta
     2401 agcggatgcc gggagcagac aagcccgtca gggcgcgtca gcgggtgttg gcgggtgtcg
     2461 gggctggctt aactatgcgg catcagagca gattgtactg agagtgcacc atatgcggtg
     2521 tgaaataccg cacagatgcg taaggagaaa ataccgcatc aggcggcctt aagggcctcg
     2581 tgatacgcct atttttatag gttaatgtca tgataataat ggtttcttag acgtcaggtg
     2641 gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa
     2701 atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga
     2761 agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc
     2821 ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg
     2881 gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc
     2941 gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat
     3001 tatcccgtat tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg
     3061 acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag
     3121 aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa
     3181 cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc
     3241 gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca
     3301 cgatgcctgt agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc
     3361 tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc
     3421 tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg
     3481 ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta
     3541 tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag
     3601 gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga
     3661 ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc
     3721 tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa
     3781 agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa
     3841 aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc
     3901 cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgttcttcta gtgtagccgt
     3961 agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc
     4021 tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac
     4081 gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca
     4141 gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg
     4201 ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag
     4261 gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt
     4321 ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat
     4381 ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc
     4441 acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt
     4501 gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag
     4561 cggaagagcg cccaatacgc aaaccgcctc tccccgcgcg ttggccgatt cattaatgca
     4621 gctggcacga caggtttccc gactggaaag cgggcagtga gcgcaacgca attaatgtga
     4681 gttagctcac tcattaggca ccccaggctt tacactttat gcttccggct cgtatgttgt
     4741 gtggaattgt gagcggataa caatttcaca caggaaacag ctatgaccat gattacgcca
     4801 agctgtaagt ttaaacatga tcttactaac taactattct catttaaatt ttcagagctt
     4861 aaaaatggct gaaatcactc acaacgatgg atacgctaac aacttgga
//
