LOCUS       sequence-212567-        6342 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Vector for expressing proteins in a subset of 9 DA/ DB cholingergic 
            motor neurons in the C. elegans ventral nerve cord.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6342)
  AUTHORS   Edwards SL, Charlie NK, Richmond JE, Hegermann J, Eimer S, Miller KG
  TITLE     Impaired dense core vesicle maturation in Caenorhabditis elegans 
            mutants lacking Rab2.
  JOURNAL   J Cell Biol. 2009 Sep 21;186(6):881-95. doi: 10.1083/jcb.200902095.
  PUBMED    19797080
REFERENCE   2  (bases 1 to 6342)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1083/jcb"; 
            journalName: "J Cell Biol"; date: "2009-09-21- 21"; volume: "186"; 
            issue: "6"; pages: "881-95"
COMMENT     Sequence Label: KG#230
FEATURES             Location/Qualifiers
     source          1..6342
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(3714..3736)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     3836..3855
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(3933..3951)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        4019..4123
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4124..4192,4193..4984)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4342..4361)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5155..5743
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5644..5663
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     5897..5914
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    6031..6052
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(6067..6072,6073..6090,6091..6097)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6105..6121
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     6110..6132
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     6129..6145
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     6129..6145
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 gtgggaatct tcggaacatg ctctttgcgg tttataagtg gagatgcgcc aacgggaaac
       61 ttgttttaaa actattccta tggtgctaac atcaccacat gaaaactttt caaaactttt
      121 ttgggttttt tattttttta ttattattcg tatctaaaca ttgtaggcgt tgtgatgttg
      181 ccctaaattt ctttaaaaag agcgaacatt ttcaaaaatt tgctatttaa atcgaatgaa
      241 aatttccatt caaatatttg aaaaaaatta tgaaaaaatt ccttttgttg catcttcatc
      301 ttctatgaaa gttacaattt tagttggatc agaaatatga gatatcaaaa aaaaattaag
      361 atttttttgt ttgttttttt gaagcctaaa atattttgat atcatttatc tcttatgtct
      421 tttgttttat cgaaacgttt acaacacatc aactatgcgt caataaattt tttaggtagt
      481 taaacaaatg ttgaaaaaaa aagcaaaagc aaccaagagc tgttaaaata gagaaaatgc
      541 agttattttt tatattcaag aaaagttatc acagaagttc cgtaatgagt tttttcctta
      601 tcaacatgtt cccatcaact tcttagtttc tcctcattcc cattgttcaa cataattatc
      661 acctctgacc ttattctgct ctttttaagc acaggtgtta atatgccgtc catcatattt
      721 gccctccgtc tgccgctttc cttatctgac ctttacatcg cctttttgta tatgtacata
      781 ataattgtat catcatcaac ctccgccata aacatgcatg tctttttacc tcttttggca
      841 tgtaccgttc ttctttgtat ttttattatg tcttccttcc atcttccctc ttttttacgg
      901 attttcggat tgcggaagaa gaagatggtc gataattacc gtaatgaggt aatttgaaac
      961 atattggaca gaagaaatac gcgcggatgc tttcggtggc aaggttaatt cgttgtcttt
     1021 gtttctggaa tgtccttcat aaaaaacacg gattaccacc tgtttttaac gtggattaag
     1081 atttttggaa acaatttact ctgcctaatt tttttgtaca tttaaaaatg tagacttacc
     1141 gaattcaccg agcttgacca gttttaaagg ttaccgttca cactaaaaat tgttgaaaaa
     1201 attttcgact aaacctaatg attttatttt aaaaaataaa tgaaatagca tacaaatttg
     1261 gatttttttc aatttttttc aagaaaatca tgaaacattt agaaaactcg agtaacaagc
     1321 tttccgttca ttctgtaatt tttaagttcc aaatttaaaa aaatgcattt tgatttactg
     1381 ggattgccgt gctcgtaatt atcagcaaaa catttgccga attagccgag ctcgacaatc
     1441 tattttttcg caaacttttg ccatgtttcg taaaacaatt ttccatttta tggtagacat
     1501 ttttcatcaa tgaattcaca aaatttaaaa tccccactga aagtaccaaa atcttcctga
     1561 aaaatccggc aaaaaatcca tctcattatc atgtcacata tacatgttct attggcacta
     1621 tgtttgggat gtcctccaat taatacttct gtgtcaatct catcggcgcc cggcggccac
     1681 taccaaatgc aaaactttgt ttgtaaaaga agaagccaat aattttctcg ttattcattt
     1741 ttattactct ttttgcgttt cattcttctt tgttcgaatt gatgaacgca cgttgcttct
     1801 ctctggtttt cctgccgttc cttatattgc caaggaaacc aatgcggatt ttcggaagat
     1861 tttcttcatg tcctttagaa aatatcacca cttttcttct ttttccattt caaatttcaa
     1921 tttccataca actcatcttc tattatagct tttcaggtgt tcgactgttt gaagataaga
     1981 atcaattaaa atttggttca ctgagcatgt catccacttt tccttttctt tgttctaaaa
     2041 ttcaccaaac tgaattttcc ttaaccggaa atttacatga aaataagaaa catggattac
     2101 atcctttaca tatcattttt gtttttttat gtgtattcca tgttcccaaa agtttgatgc
     2161 aatttttgat tctttttcaa ttttccaaat tcgtgtctcg cagggaacga ttcacagtcc
     2221 aacattccca aattgcgaaa ttttagaata tatattccca agcgtcgaat atctcaaaaa
     2281 tttgtcgttt tctcagtttg acatgtcacc tttgccggtt cctctattcg tctattagct
     2341 ctccagagag caatgtcatg tcacacgaag aattcaggga catgtttcgt ttttttttgt
     2401 ttgcatgtgg tcaccgatat gggaacttga actttccaat ttttgcttgc tttttctttt
     2461 ccggaaactt aatactattt gagaaactcc gcatcttatt tgtttgatcc caaattctga
     2521 aattcctctt tgaatttgat ctcaagcttc actataaaaa attgtttcag gaaaattgga
     2581 agagcaagcg gatccccggg attggccaaa ggacccaaag gtatgtttcg aatgatacta
     2641 acataacata gaacattttc aggaggaccc ttggctagcg tcgacggtac cggtcagata
     2701 tcactcgagg agctcccgag atcttcagcg ctcagcattc gtagaattgt aagtttaaac
     2761 tttctcatac taactaacgc gctctattta aattttcaga attccaactg agcgccggtc
     2821 gctaccatta ccaacttgtc tggtgtcaaa aataataggg gccgctgtca tcagagtaag
     2881 tttaaactga gttctactaa ctaacgagta atatttaaat tttcagcatc tcgcgcccgt
     2941 gcctctgact tctaagtcca attactcttc aacatcccta catgctcttt ctccctgtgc
     3001 tcccaccccc tatttttgtt attatcaaaa aacttctctt aatttctttg ttttttagct
     3061 tcttttaagt cacctctaac aatgaaattg tgtagattca aaaatagaat taattcgtaa
     3121 taaaaagtcg aaaaaaattg tgctccctcc ccccattaat aataattcta tcccaaaatc
     3181 tacacaatgt tctgtgtaca cttcttatgt tttttacttc tgataaattt ttttgaaaca
     3241 tcatagaaaa aaccgcacac aaaatacctt atcatatgtt acgtttcagt ttatgaccgc
     3301 aatttttatt tcttcgcacg tctgggcctc tcatgacgtc aaatcatgct catcgtgaaa
     3361 aagttttgga gtatttttgg aatttttcaa tcaagtgaaa gtttatgaaa ttaattttcc
     3421 tgcttttgct ttttggggtt tcccctattg tttgtcaaga tttcgaggac ggcgtttttc
     3481 ttgctaaaat cacaagtatt gatgagcacg atgcaagaaa gatcggaaga aggtttgggt
     3541 ttgaggctca gtggaaggtg agtagaagtt gataatttga aagtggagta gtgtctatgg
     3601 ggtttttgcc ttaaatgaca gaatacattc ccaatatacc aaacataact gtttcctact
     3661 agtcggccgt acgggccctt tcgtctcgcg cgtttcggtg atgacggtga aaacctctga
     3721 cacatgcagc tcccggagac ggtcacagct tgtctgtaag cggatgccgg gagcagacaa
     3781 gcccgtcagg gcgcgtcagc gggtgttggc gggtgtcggg gctggcttaa ctatgcggca
     3841 tcagagcaga ttgtactgag agtgcaccat atgcggtgtg aaataccgca cagatgcgta
     3901 aggagaaaat accgcatcag gcggccttaa gggcctcgtg atacgcctat ttttataggt
     3961 taatgtcatg ataataatgg tttcttagac gtcaggtggc acttttcggg gaaatgtgcg
     4021 cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca
     4081 ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt
     4141 ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga
     4201 aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga
     4261 actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat
     4321 gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtattg acgccgggca
     4381 agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt
     4441 cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac
     4501 catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct
     4561 aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga
     4621 gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgtag caatggcaac
     4681 aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat
     4741 agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg
     4801 ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc
     4861 actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc
     4921 aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg
     4981 gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta
     5041 atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg
     5101 tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga
     5161 tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt
     5221 ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag
     5281 agcgcagata ccaaatactg ttcttctagt gtagccgtag ttaggccacc acttcaagaa
     5341 ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag
     5401 tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca
     5461 gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac
     5521 cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa
     5581 ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc
     5641 agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg
     5701 tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc
     5761 ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc
     5821 ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag
     5881 ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc caatacgcaa
     5941 accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca ggtttcccga
     6001 ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc attaggcacc
     6061 ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga gcggataaca
     6121 atttcacaca ggaaacagct atgaccatga ttacgccaag ctgtaagttt aaacatgatc
     6181 ttactaacta actattctca tttaaatttt cagagcttaa aaatggctga aatcactcac
     6241 aacgatggat acgctaacaa cttggaaatg aaataagctt gggctgcagc ccgggaaaca
     6301 tgatatcgac ggacatattt ttgattgatt atcataattt tc
//
