LOCUS       sequence-212568-        6930 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses the C. elegans pde-4 (isoform d) D448N cDNA 
            pan-neuronally.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6930)
  AUTHORS   Charlie NK, Thomure AM, Schade MA, Miller KG
  TITLE     The Dunce cAMP phosphodiesterase PDE-4 negatively regulates G 
            alpha(s)-dependent and G alpha(s)-independent cAMP pools in the 
            Caenorhabditis elegans synaptic signaling network.
  JOURNAL   Genetics. 2006 May;173(1):111-30. doi: 10.1534/genetics.105.054007. 
            Epub 2006 Apr 19.
  PUBMED    16624912
REFERENCE   2  (bases 1 to 6930)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1534/genetics.105.054007"; journalName: "Genetics"; date: 
            "2006-05"; volume: "173"; issue: "1"; pages: "111-30"
COMMENT     Sequence Label: KG#205
FEATURES             Location/Qualifiers
     source          1..6930
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(4366..4388)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     4488..4507
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(4585..4603)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        4671..4775
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4776..4844,4845..5636)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4994..5013)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5807..6395
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6296..6315
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     protein_bind    6683..6704
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(6719..6724,6725..6742,6743..6749)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6757..6773
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     6762..6784
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     6781..6797
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     6781..6797
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 ttgggctgca ggatcttcag atgggagcag tggactgtct atttgatatt cttaaaatgt
       61 actctataaa agatgaaact gtaatgacca attccctaca taaatatttt taactaattg
      121 gtcggatatt tggggatcag agaagtactt gtttttgcga tattaataat gatatatttt
      181 tctagtaaaa aaatgttaag cgatttgtga ataattaagt tggcttgaaa aagatttttt
      241 gatagctatg tattttgtta atttaaatca gattttaaaa ctttattatt gactatttat
      301 atatattata tattatatat tttcatggtt taatcaaaat ctattaaatt atttctaaaa
      361 tctctaaata tcgaattctc taaaaagctt catcaacaaa agcatttcaa ttatctaaca
      421 caatgagaat aattcaagaa ctgaaataga aagaaatcta gaaaaaaaag agagtgaggt
      481 gccctattaa atcggtgctc tcctctctaa cagaagcgcc tacacgcacc caagagagct
      541 ctttttcttt cttttttcgg agcacacata cacgcataca ctcctcagaa gtgtatagag
      601 aaaatgggag aagaagcgaa agaagacgaa gaagcggttg tgatgtatgc atcgggcgtt
      661 tctatctctc tccgtgagca acgagctagt caacccatcg atccattttt gtgagcacac
      721 acagagagag actcaaacac actcacagct tcacactgag ctccaagagc tctgtagaga
      781 gaagccagag tatgctttcc tcgattttcc taacgtcttc ttcttctgct tttttctttt
      841 tcccaacgag ttgtagtcca ctaacttcaa tttttcctcc agagaaagag ttttagagag
      901 acagctgata aatacactag agaaataaaa agaatgataa aaatttgcta ttttaatcag
      961 ttccctctcg tttctctaag ttttgttgtt gggttgggaa attaaaattg gaaaatggaa
     1021 ataacgtttt tttctgtcta atcttgaatt ctccagacat ttcttatttc cttttttgtt
     1081 gcaagttttt cttccttctc taatttttaa tcaaagatat ctcgctatga actttgaatt
     1141 tttgatattt tgattctata aatacataaa gtaatctaaa tgctctttta aaataaatct
     1201 acagtagccc tattttcagg gatccccggg attggccaaa ggacccaaag gtatgtttcg
     1261 aatgatacta acataacata gaacattttc aggaggaccc ttggctagca aaaatgccac
     1321 gaagacgcgg ctcgtcgtcg tcgtcgtcgg cggcaggtgg tagtggcggc ggcggaggct
     1381 tcggattctc gagcctccga cgcgaactcc acttgcacaa cttctttcga acctcttcgc
     1441 cgtcggcctc gtcgacgtcg agaacaccgc ccgcagcatt accaccgcgc acatcagctg
     1501 ttactattcc gggcagtaat cacaaattga cctcttccgc ttcatcttac cacccaccac
     1561 gcgagcttac cgtgtcgacg ttcagcgccg gctcggcaac tgcagctgat ggacttggag
     1621 gtgctcatct aacgccatca ctttcatctt cggtacatgc gagacgggag tcgtttctct
     1681 accgtgccag tgatgatctt cgagaagcgt cttcgttacg accagtcagt cgggcgtcgt
     1741 caatcgcgtc caatgagcac ggacacggtg acgacctgat tgtaaccccg tttgctcaac
     1801 tgcttgccag tcttcgaaac gttcgatcca atctgatttc gataaccaac atccagaaca
     1861 gcgacgacag tcgacacgct aatcgaagtg cgaaacggcc gcctttgcac aacattgagc
     1921 ttccagacga tgtggtgcat tgtgcgcatg atacactcga agaactcgat tggtgtcttg
     1981 atcagctgga aacaattcaa actcatcgat cagtgtctga gatggccagc agtaagttcc
     2041 gcaagatgct caacaaagag ttgtcacact ttgctgaatc gtccaaatcc ggcactcagg
     2101 tctccaagtt tttgatcacc acctacatgg acaaggaaga ggatgaaccg tcgatcgaga
     2161 tcgaagtgcc cactgaggta caaggccctt ctacatctgg accaatgaca ctttcgatac
     2221 tgaaaaaagc tcaaacagca gccatgaata agatctcagg agttcgaaaa cttcgtgctc
     2281 catcacatga cggccatgtt cccgaatacg gtgtaaactg tgcacgtgag atagctgttc
     2341 atatgcaacg actcgatgac tggggacctg atgtcttcaa aatcgacgaa ctttccaaaa
     2401 atcactcgct cactgtcgtt acattttcat tactccgcca gcggaatctc tttaaaactt
     2461 ttgaaattca tcaatctact ctggtcacct acttgctcaa tcttgaacat cactatcgta
     2521 ataatcatta tcacaatttt attcatgcag ccgatgttgc acagtcaatg cacgttctgc
     2581 tcatgtctcc cgtccttaca gaagtcttca ctgatcttga agtattagca gctatatttg
     2641 ccggtgctgt ccataatgta gatcatcctg gattcacgaa tcagtatttg atcaactcaa
     2701 acaatgaatt agcgatcatg tacaatgatg aaagtgtcct tgagcagcat catctggcag
     2761 ttgcattcaa gcttttgcaa gattcaaact gtgatttcct tgcaaatttg tccagaaaac
     2821 aacggctgca atttcgcaag attgtcattg atatggtact tgcaactgat atgtctaagc
     2881 acatgtctct tcttgctgat ctgaaaacaa tggtcgaagc aaagaaagtt gctggaaaca
     2941 atgtgattgt gctggacaag tacaatgaca aaattcaggt actacaatcg atgattcatc
     3001 tcgctgatct ctcaaatcca actaagccaa tcgaattata ccaacaatgg aatcaaagga
     3061 taatggaaga atactggagg cagggagata aagagaaaga gcttggactt gagatatcac
     3121 cgatgtgcga tcgaggaaat gtgaccattg aaaaatcaca ggtcggattc atcgactaca
     3181 ttgttcatcc attatacgaa acatgggcag atcttgtcta tccagatgct caaaatatac
     3241 tcgatcaact cgaagaaaat cgagaatggt accaatcacg aatacctgaa gaacctgaca
     3301 cagctcgtac tgtaacagaa gatgacgagc acaaataaat accggtcaga tatcactcga
     3361 ggagctcccg agatcttcag cgctcagcat tcgtagaatt gtaagtttaa actttctcat
     3421 actaactaac gcgctctatt taaattttca gaattccaac tgagcgccgg tcgctaccat
     3481 taccaacttg tctggtgtca aaaataatag gggccgctgt catcagagta agtttaaact
     3541 gagttctact aactaacgag taatatttaa attttcagca tctcgcgccc gtgcctctga
     3601 cttctaagtc caattactct tcaacatccc tacatgctct ttctccctgt gctcccaccc
     3661 cctatttttg ttattatcaa aaaacttctc ttaatttctt tgttttttag cttcttttaa
     3721 gtcacctcta acaatgaaat tgtgtagatt caaaaataga attaattcgt aataaaaagt
     3781 cgaaaaaaat tgtgctccct ccccccatta ataataattc tatcccaaaa tctacacaat
     3841 gttctgtgta cacttcttat gttttttact tctgataaat ttttttgaaa catcatagaa
     3901 aaaaccgcac acaaaatacc ttatcatatg ttacgtttca gtttatgacc gcaattttta
     3961 tttcttcgca cgtctgggcc tctcatgacg tcaaatcatg ctcatcgtga aaaagttttg
     4021 gagtattttt ggaatttttc aatcaagtga aagtttatga aattaatttt cctgcttttg
     4081 ctttttgggg tttcccctat tgtttgtcaa gatttcgagg acggcgtttt tcttgctaaa
     4141 atcacaagta ttgatgagca cgatgcaaga aagatcggaa gaaggtttgg gtttgaggct
     4201 cagtggaagg tgagtagaag ttgataattt gaaagtggag tagtgtctat ggggtttttg
     4261 ccttaaatga cagaatacat tcccaatata ccaaacataa ctgtttccta ctagtcggcc
     4321 gtacgggccc tttcgtctcg cgcgtttcgg tgatgacggt gaaaacctct gacacatgca
     4381 gctcccggag acggtcacag cttgtctgta agcggatgcc gggagcagac aagcccgtca
     4441 gggcgcgtca gcgggtgttg gcgggtgtcg gggctggctt aactatgcgg catcagagca
     4501 gattgtactg agagtgcacc atatgcggtg tgaaataccg cacagatgcg taaggagaaa
     4561 ataccgcatc aggcggcctt aagggcctcg tgatacgcct atttttatag gttaatgtca
     4621 tgataataat ggtttcttag acgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc
     4681 ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct
     4741 gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg
     4801 cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg
     4861 tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc
     4921 tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca
     4981 cttttaaagt tctgctatgt ggcgcggtat tatcccgtat tgacgccggg caagagcaac
     5041 tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa
     5101 agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg
     5161 ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt
     5221 ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg
     5281 aagccatacc aaacgacgag cgtgacacca cgatgcctgt agcaatggca acaacgttgc
     5341 gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga
     5401 tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta
     5461 ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc
     5521 cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg
     5581 atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt
     5641 cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa
     5701 ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt
     5761 cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt
     5821 ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt
     5881 tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga
     5941 taccaaatac tgttcttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag
     6001 caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata
     6061 agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg
     6121 gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga
     6181 gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca
     6241 ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa
     6301 acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt
     6361 tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac
     6421 ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt
     6481 ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga
     6541 ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cccaatacgc aaaccgcctc
     6601 tccccgcgcg ttggccgatt cattaatgca gctggcacga caggtttccc gactggaaag
     6661 cgggcagtga gcgcaacgca attaatgtga gttagctcac tcattaggca ccccaggctt
     6721 tacactttat gcttccggct cgtatgttgt gtggaattgt gagcggataa caatttcaca
     6781 caggaaacag ctatgaccat gattacgcca agctgtaagt ttaaacatga tcttactaac
     6841 taactattct catttaaatt ttcagagctt aaaaatggct gaaatcactc acaacgatgg
     6901 atacgctaac aacttggaaa tgaaataagc
//
