LOCUS       sequence-212571-        9846 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses the C. elegans acy-1 P260S gain-of-function cDNA in body 
            wall muscle.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9846)
  AUTHORS   Schade MA, Reynolds NK, Dollins CM, Miller KG
  TITLE     Mutations that rescue the paralysis of Caenorhabditis elegans ric-8 
            (synembryn) mutants activate the G alpha(s) pathway and define a 
            third major branch of the synaptic signaling network.
  JOURNAL   Genetics. 2005 Feb;169(2):631-49. doi: 10.1534/genetics.104.032334. 
            Epub 2004 Oct 16.
  PUBMED    15489510
REFERENCE   2  (bases 1 to 9846)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1534/genetics.104.032334"; journalName: "Genetics"; date: 
            "2005-02"; volume: "169"; issue: "2"; pages: "631-49"
COMMENT     Sequence Label: KG#81
FEATURES             Location/Qualifiers
     source          1..9846
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(7290..7312)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     7412..7431
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(7509..7527)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        7595..7699
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7700..7768,7769..8560)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7918..7937)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8731..9319
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9220..9239
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     9473..9490
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    9607..9628
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(9643..9648,9649..9666,9667..9673)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9681..9697
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     9686..9708
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     9705..9721
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     9705..9721
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 aaataagctt gggctgcagg tcggctataa taagttcttg aataaaataa ttttcccgac
       61 aaaacatgag tatttctttc gaaaataaaa gtgcaggcta attagagatt attctgtaat
      121 taactgcata atttgtcacg tgccatagtt ttacattcca ctacgtcata gttcttaaaa
      181 tactaatctc ctgaaaatag aagtaggtga agaaagttta attatcagtt ctaaaatgac
      241 aattgatctt tggaatatgt tctgaaacta ccgatcattg aacagatgct atttgaatga
      301 tatagaattg tatatttgca atttctgaaa cgcgttctta aaggcacaca gattaattca
      361 aaagggtctg gccgcaaaaa ggtttatggt ggccgatttt gagttttgtg tgtgattgct
      421 ttttcacaat cagtgttttc aggattatgt gatgaactag atcttcaagt ttcgttacat
      481 ttcatatgtt ttcggaactc acgaagtaca tattgggtat tgtgctcaaa aaattcagca
      541 atcagcttcg ctccgctgac tttagaaccc aaaaaaatag tatggccaaa ctgactgtgt
      601 tacgatcatt tcaatttttc aatacatatt taagatttct aagagtaaga aggtcaaaaa
      661 ctgttctgga atacatatat atttttcagg ttacaattag tcaaaaagtg cactgaaata
      721 tacgttttaa tttcacgaat aacccaatta gttcaatgta tttttggtca accaacgtta
      781 aagtttggct tccaaccaat tatcatttct gatcaaccac aatgtttttt ctttatctgc
      841 aagttaattt tttattttta tccagatgtt tggcatattt ttcaattctt cactagcgcc
      901 cacttcttgc acttccggcg ccctgaatct aatgcatctg ttgcaagaat tgaaagacca
      961 atcaacacat tgttttcttc acgagatact gaagaaaatg aataaaaaca gagaaaaaga
     1021 gccatgtgat tagtgacaac tgttgctaac agataccata gcttggactt ggtacgtgat
     1081 ggcaacgtat gggtcaacaa aaatgattgc agagggggtg caaaacagtc aagtcgagaa
     1141 aatatgaaaa acagaaaaca aagaacagaa aaatgggttt gagagtcagt ataatttata
     1201 aaagaaaaat tgtacataga aattaaccat ttttgtagaa gaagttattt ttcaagcatc
     1261 gttaaaaatt attcaaagca ccttatttca tatttaattt taaacatggt taaatgaaca
     1321 acacggtgcg caatcaggaa aacttgaaat ctgaaactgt tgttgtgatc ttcttcgcaa
     1381 ctgttcagat agcactagtg taatgttaag agtgcgcgaa tataatggaa tataatggat
     1441 cacacctcct gccatcaggt aaacgtctct gttatcacat atttccaact attaaatttt
     1501 taccttttac agttttacat ttttttgaaa aaagtaactt tttgtcttca aaatccctga
     1561 cgaaaatatc aaatatttta atcgagactg cagaggaacc gattgatgat ttggaaaatc
     1621 cagctttacc tgtgtaagaa ctgaaaagtt tcataaccct agggtattcc cagttacatt
     1681 ccccactggc taacaatagc acccagtttt tcatcacctt tcttcaaatt tctcggcgat
     1741 ttgttaaaaa caaaatttgt gtcccttctc tgatatctct atgtctctaa acacaagttc
     1801 atcggaaaac gaaggagggt aggtgttggt tgggctcccg aagtgaaaat agaagagcaa
     1861 gaatagaata ttagagagag agtgcagaga gggcgggata gctcccggga ttccgttttc
     1921 ttcttcttta tcttcaacga tgatgtgtgt gcgtgttgta tagattctgt tgctccccca
     1981 caactcgctc cgaaggctca atacaattca attgatattg gaggagagcc taccggagtg
     2041 ggaggataag aagaaacata agaagaagaa gaagaagaag catgcttctg gtttttgatg
     2101 ctatgaaaac ggcacaaaaa gatgattgag gtcccttttc aataccttct ctcatctttc
     2161 aaatcccatt gaaacctaaa acttctcacc acgctttacc attgttctcc aaaaacttat
     2221 agcaatgtct ataacttttt tatctctgaa aagcagtgtt ccatttttct ttttcctatt
     2281 ttatttcaat tgtttctcac atttcgtttg gattctttgc ttgtcaacca gcttcttctt
     2341 ccacttttac cgtctaattt tcagggcagg gagccatcaa acccacgacc actagatcca
     2401 tctagaggat ccccgggatt ggccaaagga cccaaaggta tgtttcgaat gatactaaca
     2461 taacatagaa cattttcagg aggacccttg gctagcgtcg acggtaccgg taaaaatgga
     2521 cgacgatgtc ggtgaacgaa caccggctct cggtggttca tgtggaccat cagttcgtgc
     2581 acacagtagt tccccaagac gtgttccatt atttgagaga gcatctgcta gatggtggaa
     2641 tcctcaattt cgatctgcaa cactagaagc tcaatattgg aagtgtagtt tcagtcagtt
     2701 aagagatcga tttcgttctg gattaatata tattgccgtt gtaattgccg cttggacact
     2761 ttatttagct ttattcgata gaacttttat tcagcattgg atcgtttcac tatgcctctg
     2821 tgccattatt ttcgcgatgt ttgccttcac ggcgtgcgcc gctcaatacc aaaggttcta
     2881 catgccaacc tcatttctat gcactttttt gatatgcctt gtcacgttgc tcatcttctc
     2941 cgcagagaat caagctgcgt ttatgactcc agttgcaagt cttgccacaa gttttcaggt
     3001 cgttctcctg atctacacag tcattccact gccactctat ttatgtatac tcatcggaat
     3061 aatctactca atcctatttg agattctcaa caagaacaaa attggtcttg aagaggccgg
     3121 atacattaag cttgtccttc acgctggtgt ccatttactc ggtgtccatt tgttcatttt
     3181 aactcaagtg cgacagagga aaacgtttct gaaagtggga caatcgatgt tggcaagaaa
     3241 ggatctagag ttggagacac agtttaaaga tcatatgatt cagtctgtga tgtctaaaaa
     3301 ggtagctgat gaattactga aagatgcatc agaacttcgc cgaccttctg cttccaacga
     3361 ctcaaactgc cgaacttcaa atgctacaca agttgaccag ccccttgcca agatggtacc
     3421 tgaataccga aagttccgtc catttacgat gaatctcatg acaaatgtgt cgattctgtt
     3481 tgcggatatt gctgggttta ctaaaatgtc atcaaataaa tctgccgacg aacttgtcaa
     3541 tctgctcaat gatctttttg gaagattcga tactttgtgt cgattgagag gattagagaa
     3601 gatttcaact ttgggagatt gttactattg tgttgctgga tgtcctgaac catgtgatga
     3661 tcatgcatgc agaacagtcg aaatgggtct cgacatgatt gttgcaatcc gccaatttga
     3721 tattgatcgt ggacaagaag tgaatatgcg tgtgggaatt catacaggaa aagtaatgtg
     3781 cggaatggtt ggaacaaaaa gattcaaatt cgatgtgttc tcaaacgatg tcacactggc
     3841 aaatgagatg gaatcttctg gtgttgctgg aagagttcac gtttccgaag caactgcaaa
     3901 acttttgaaa gggctttatg aaattgaaga gggaccggat tatgatggtc cattgaggat
     3961 gcaagttcag ggaactgaac gtcgtgttaa accagaaagc atgaagactt tctttatcaa
     4021 aggacgtatt aatgatggtg tagaagaaga agtgatgcaa gttcaggaag ttgagagcct
     4081 tcacagtcag aaaagcagta aaaagtcgac tctaaaacag aaatgggcgg aaaagttgaa
     4141 aatgaaccac acaaattctt atccaatgcg agccgctgca cgtgaaggag gtggaagttt
     4201 gagaataaaa ttggcagaga gaaatagaag tacacagtta ctgccaaaag aatcaaattc
     4261 aatttgtata atggaagata accgaaagtc agcatcactt caagcattgg caacgaataa
     4321 ttttaatgga agtaatacag atacaaataa cacttatagt gaacgtggag ttgctggaag
     4381 tgttagtaag aagtctgtag ctggaagtga aagtaatagt ataaaaggaa gtagaagcag
     4441 tggactacaa ttatctctac aagatggaaa ttctgattta aattcagttg gaggtttgga
     4501 tactgcaatc agtcaccatc acaatgcagc atctctcact cgatttgata ctgataacaa
     4561 ttttgatcag cgtttggcaa tggtaattgg acaaggagaa ggaggatttg ataaaggatt
     4621 ttggaatcat catgatagtt tgaataagtg gactcttcga tttaatgaaa aggacgttga
     4681 agaggaatat cgagctcatt ttgtggattc atcggaaaga tatacggcat caaagaaagg
     4741 tcatgttgaa agacataaag atttgatgga acaaggtgga gaaaaagatg gaattactgg
     4801 atcaacggtc aacaagtata gatattcagg agtcttcatc gatataattg ttgccacgtt
     4861 gatatttgta atatcgggtg cagttgcaat tatgtctgtc cgtccattcc cattatcact
     4921 cattgcatac ttcccatttg ctgctgcaat tctcattctt acaattgtcc ttattggtct
     4981 tccacttctc gcccgtaaaa aatcattcca atgtgcaaat caatggatgc cacgtcatct
     5041 catcggtctt cttctgatct ttcttccaat cggtgtcgca atctgtatca tgccattgtg
     5101 ccaaagtgga gactgtgcaa atgtaattct gaactaccgt cttgcattca gttatgtcac
     5161 cattcttgcc atattcgctc attgcaactt ctcccaatta gctgcctggc caaaaactac
     5221 tgctgcagta tttatcggac ttctccacat tgctggagtt ttctactgcg aattcaattt
     5281 gaaacactta gttgaagagc aagacacctg caacgtgaca gcaatcatga ttccaccaat
     5341 tcgcaaaggt ctgaactaca caattgcatt gaactcgaca tctgcaagaa cattatctca
     5401 agattttgga tctccattat tcatttggga gctgcttctg gatgtgatcc tgtcgattgt
     5461 tctagttgca ttccttaatt atcagttcga aaccgcattc cgtatgtcat tctttggaga
     5521 tgttcaagct cgtcgggaca ctgaaagaat gcaaatcgtg cgtgatcaag ccgattggct
     5581 cctgaataac gtgatcccag cccacgcagt agaaagtctt aaaacggata ccaaatacag
     5641 tgaaaaccat gaaacagttg gtgtactgtt cgcttcaatc actaattgga atgatatgta
     5701 tgaagagaat ttcgaagggg gcagagaatt cttgagagtt ctcaatgaag ttattggtga
     5761 ctttgatgag cttctcgatc ggccggactt cacgcatatt gaaaaaatca aaacaattgg
     5821 gcctgcgtat atggcagcaa gtggattgaa tccagaacga aaaaagaata tgttgcatcc
     5881 aaaagagcat ctatatcaga tggttgattt tgcattggca gttcaacatg tactcagtgt
     5941 tttcaacgag gatttgctca attttgattt tgtttgcaag ttaggactta atattggacc
     6001 cgtcactgct ggtgtcattg gaacaacaaa gttgtactac gatatctggg gtgacactgt
     6061 aaacattgct agccgaatgt acagcaccgg agttttaaat cgcattcaag tatcacaaca
     6121 cacacgagaa tatctcctgg atcggtatga attcgagttc cgcgatcata ttgaagtcaa
     6181 gggaattgat ggaggaatgg atacttatct tttggttggt cgaaaaggtg atggaatacc
     6241 tccatcaatc aaggataacc aagaagacga attttaaatc tcgaggagct cccgagatct
     6301 tcagcgctca gcattcgtag aattgtaagt ttaaactttc tcatactaac taacgcgctc
     6361 tatttaaatt ttcagaattc caactgagcg ccggtcgcta ccattaccaa cttgtctggt
     6421 gtcaaaaata ataggggccg ctgtcatcag agtaagttta aactgagttc tactaactaa
     6481 cgagtaatat ttaaattttc agcatctcgc gcccgtgcct ctgacttcta agtccaatta
     6541 ctcttcaaca tccctacatg ctctttctcc ctgtgctccc accccctatt tttgttatta
     6601 tcaaaaaact tctcttaatt tctttgtttt ttagcttctt ttaagtcacc tctaacaatg
     6661 aaattgtgta gattcaaaaa tagaattaat tcgtaataaa aagtcgaaaa aaattgtgct
     6721 ccctcccccc attaataata attctatccc aaaatctaca caatgttctg tgtacacttc
     6781 ttatgttttt tacttctgat aaattttttt gaaacatcat agaaaaaacc gcacacaaaa
     6841 taccttatca tatgttacgt ttcagtttat gaccgcaatt tttatttctt cgcacgtctg
     6901 ggcctctcat gacgtcaaat catgctcatc gtgaaaaagt tttggagtat ttttggaatt
     6961 tttcaatcaa gtgaaagttt atgaaattaa ttttcctgct tttgcttttt ggggtttccc
     7021 ctattgtttg tcaagatttc gaggacggcg tttttcttgc taaaatcaca agtattgatg
     7081 agcacgatgc aagaaagatc ggaagaaggt ttgggtttga ggctcagtgg aaggtgagta
     7141 gaagttgata atttgaaagt ggagtagtgt ctatggggtt tttgccttaa atgacagaat
     7201 acattcccaa tataccaaac ataactgttt cctactagtc ggccgtacgg gccctttcgt
     7261 ctcgcgcgtt tcggtgatga cggtgaaaac ctctgacaca tgcagctccc ggagacggtc
     7321 acagcttgtc tgtaagcgga tgccgggagc agacaagccc gtcagggcgc gtcagcgggt
     7381 gttggcgggt gtcggggctg gcttaactat gcggcatcag agcagattgt actgagagtg
     7441 caccatatgc ggtgtgaaat accgcacaga tgcgtaagga gaaaataccg catcaggcgg
     7501 ccttaagggc ctcgtgatac gcctattttt ataggttaat gtcatgataa taatggtttc
     7561 ttagacgtca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt
     7621 ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata
     7681 atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt
     7741 tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc
     7801 tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat
     7861 ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct
     7921 atgtggcgcg gtattatccc gtattgacgc cgggcaagag caactcggtc gccgcataca
     7981 ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg
     8041 catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa
     8101 cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg
     8161 ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga
     8221 cgagcgtgac accacgatgc ctgtagcaat ggcaacaacg ttgcgcaaac tattaactgg
     8281 cgaactactt actctagctt cccggcaaca attaatagac tggatggagg cggataaagt
     8341 tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg
     8401 agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc
     8461 ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca
     8521 gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc
     8581 atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat
     8641 cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc
     8701 agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg
     8761 ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct
     8821 accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa atactgttct
     8881 tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct
     8941 cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg
     9001 gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc
     9061 gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga
     9121 gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg
     9181 cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta
     9241 tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg
     9301 ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg
     9361 ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat
     9421 taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc
     9481 agtgagcgag gaagcggaag agcgcccaat acgcaaaccg cctctccccg cgcgttggcc
     9541 gattcattaa tgcagctggc acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa
     9601 cgcaattaat gtgagttagc tcactcatta ggcaccccag gctttacact ttatgcttcc
     9661 ggctcgtatg ttgtgtggaa ttgtgagcgg ataacaattt cacacaggaa acagctatga
     9721 ccatgattac gccaagctgt aagtttaaac atgatcttac taactaacta ttctcattta
     9781 aattttcaga gcttaaaaat ggctgaaatc actcacaacg atggatacgc taacaacttg
     9841 gaaatg
//
