LOCUS       sequence-212573-       14905 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of a fungal biosynthetic gene cluster.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 14905)
  AUTHORS   Harvey CJB, Tang M, Schlecht U, Horecka J, Fischer CR, Lin HC, Li J,
            Naughton B, Cherry J, Miranda M, Li YF, Chu AM, Hennessy JR, Vandova
            GA, Inglis D, Aiyar RS, Steinmetz LM, Davis RW, Medema MH, Sattely 
            E, Khosla C, St Onge RP, Tang Y, Hillenmeyer ME
  TITLE     HEx: A heterologous expression platform for the discovery of fungal 
            natural products.
  JOURNAL   Sci Adv. 2018 Apr 11;4(4):eaar5459. doi: 10.1126/sciadv.aar5459. 
            eCollection 2018 Apr.
  PUBMED    29651464
REFERENCE   2  (bases 1 to 14905)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Adv."; 
            date: "2018-04-11"; volume: "4(4):eaar5459. doi"; pages: " 
            10.1126/sciadv.aar5459. eCollection 2018 Apr"
COMMENT     Sequence Label: pCHSU75.1-2.2
FEATURES             Location/Qualifiers
     source          1..14905
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             264..287
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             complement(1744..1755)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(9089..9109)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(9089..9107)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     rep_origin      complement(9446..10034)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9526..9545)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(10205..10996,10997..11065))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10828..10847
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(11066..11170)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      11197..12539
                     /label=2u ori
                     /note="yeast 2u plasmid origin of replication"
     protein_bind    complement(12160..12207)
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     primer_bind     12580..12598
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        12893..13300
                     /gene="S. cerevisiae LEU2"
                     /label=LEU2 promoter
     CDS             13301..14395
                     /codon_start=1
                     /gene="S. cerevisiae LEU2"
                     /product="3-isopropylmalate dehydrogenase, required for 
                     leucine biosynthesis"
                     /label=LEU2
                     /note="yeast auxotrophic marker"
                     /translation="MSAPKKIVVLPGDHVGQEITAEAIKVLKAISDVRSNVKFDFENHL
                     IGGAAIDATGVPLPDEALEASKKADAVLLGAVGGPKWGTGSVRPEQGLLKIRKELQLYA
                     NLRPCNFASDSLLDLSPIKPQFAKGTDFVVVRELVGGIYFGKRKEDDGDGVAWDSEQYT
                     VPEVQRITRMAAFMALQHEPPLPIWSLDKANVLASSRLWRKTVEETIKNEFPTLKVQHQ
                     LIDSAAMILVKNPTHLNGIIITSNMFGDIISDEASVIPGSLGLLPSASLASLPDKNTAF
                     GLYEPCHGSAPDLPKNKVNPIATILSAAMMLKLSLNLPEEGKAIEDAVKKVLDAGIRTG
                     DLGGSNSTTEVGDAVAEEVKKILA"
     primer_bind     complement(14642..14658)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(14642..14658)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
ORIGIN
        1 atttcagatt gagagaatga aaaaaaaaaa aaaaaaaagg cagaggagag catagaaatg
       61 gggttcactt tttggtaaag ctatagcatg cctatcacat ataaatagag tgccagtagc
      121 gacttttttc acactcgaaa tactcttact actgctctct tgttgttttt atcacttctt
      181 gtttcttctt ggtaaataga atatcaagct acaaaaagca tacaatcaac tatcaactat
      241 taactatatc gtaatacaca atggattata aggatgatga tgataagact agtatggttt
      301 caactccatc ctctgccatt cgccccagct atgccaccac tgactctcat gccatggcca
      361 tcgtcggtac ctccatcgct gccccaggcg gcgtcgacga tggactagac actgaggagt
      421 tctatgagtt cctcaaatct cgtggctccg gtatcatcac tgtacccaaa gatcgctgga
      481 atgctgaagc ttatcatggc gctcaacctg gcaaaattgt tactaccaaa ggtggttaca
      541 tcccaaacgc tacatacgga gacacgcaag aattcggtat cactcctgca gaagctgccc
      601 agatggctgt ttctcaaata gctttgcttc accacgcctt caacgctttg caacgcagtg
      661 gagtcgacta ccgaggcacg aacactggcg tgtatgtcgg ttgcacgact ggtgcagcac
      721 cccacaacgt agacatcacc gaagccggag catactacat gaccggtagc tcgatatcta
      781 tcaatgcgaa tcgtatcagc tacgttttcg acctgactgg tccttccttg ccagtcgata
      841 cggcctgctc ttctagtttg accgctatgc atctcgctgt gcaagctgtt aggaatggag
      901 aatgtgacca agctgttgtt gctggagtca acatccttct cagtccactt gagactgcat
      961 ccttcagtca gctcggagtc ctcggcccag atggtatctc gaaagctttc gatgaagatg
     1021 caaacggtta cgcgcgtggt gatgttgtca gtgccgtggt tatcaagcgt cacgatcttg
     1081 ctgtcaggga ctatgaccgt attctggcca ctctcgtcgg cactgctctt acgtcctgcg
     1141 gatccttgat gggatctctc actacaccta gccccgatgc tcaaaccaag gctattagga
     1201 atgcctacgc cgatgctggt ctaacaccca accaagccga cttcgttgag ttacacggta
     1261 ctggaactat cgtcggagat cagtacgagg ccaatacggc aggcgcatgc ttctctgacg
     1321 gacgtgacgg tcgagaaatc gttatcggct cagtcaagag caacgtcggt cacggtgaaa
     1381 tgggtgctta tatgagctct ttggtcaaag ttgtcatgat gttggaacgc aagcagattc
     1441 ttccgaacgg atacttcaag aagccttctt cgaggatcca atttgacgag tataagctgc
     1501 gggtacctat cgaagtcgag gactttgttc cctatgacgt cgagcagggt ctaatcgcgt
     1561 cgatttcttc cttcggattc ggaggatctt gtggtcacac tgtcctgcga gaacacgatg
     1621 ttaggcccgt cctccctgac tacgaaacgc taaactcggg accatatctc ttcgccgttg
     1681 gaggtcttac tcctcgttca gtcaacactc tcatcgagag ctacaaagcc gagtacgcgg
     1741 atgtcgaccc tctatcccta tctgaacacc tcggctcgcg tgctcgccaa ttgagctggc
     1801 gttcattcgc agttgcagat tccttgggcc aggcaaagtt cactgaacct gtcatggtat
     1861 caaaacgtgc tgttcctttg atcttttgct tctctggtca aggccctcag cactggcaac
     1921 agggtcgcga actcttctcc cgatactctg tcttccgtga aagtatcttg gcttccgaca
     1981 aaatttacac cgagtacaca ggaaagtcct tccttgaaac ctccggcctc tttgtcccca
     2041 accctcctgc gaactctatc ctcgccaagt ccctttcatg gcccgcagac gccatctctg
     2101 tttccatcac tttcttccag atcgctcttt tcgacctttc gatctacctt ggactcaagc
     2161 ccgacgccat cgttggacac agtatcggcg aaactgctgt tctttatgcc agtggtgcta
     2221 tgcctcgcga gatggtcgtc aagatcgccg ttgctcgagg tgctgctctt cgcctcgttg
     2281 acaacattgg tggagccatg gtcgctgtct ctggttgcga cctccgaaca gtccaagatt
     2341 atgccgaagc agtcgttgac ctttccaaca acccgacggc gggtgttacc gactctctcc
     2401 acgtggcagc tcggaatagc ccgaccgact ttggtctgtc gggtgctgag tatctcgtcg
     2461 acgaactggc caagtatatc gaccaatggg tttctggagt ctctgctcgc aagcttcgtg
     2521 tcagcactgc tgttcactct ccttatgtcg atgcttgcga ggcccaatac cgtcaagagc
     2581 tcgctgcaat ctttgaagca caccctggta cccacaagcc ttcgcttcca gtgatgtcaa
     2641 cagtgacagc cgagttcgtt gagaaggagt acacaatcga ctacctctgg aacaaccttc
     2701 gtcaacctgt tcgcttctct gatgctattc ccaagctcat cgagaagcac ggtgaaaaca
     2761 cgaccttcgt tgagattgct cctcatcccg tcttgtctca atacatcaag caaatgggtg
     2821 ccttcgagtc ggtcgcgggt agcaaacgtc ctccttcggc tcgtcacatc aaggctggtc
     2881 aacgccctcc taccgagctc gatgcactgt acgaagggat tggccagctg ttggcttcag
     2941 gtgtcaactc ggtcaacttt gcactcctta acggttgtcc tccagagaac ttccttggac
     3001 ctaagtaccc attccaaatg aagtacttcc cggtcgcacc tcgtgttccc agctatgtgc
     3061 gcaagctgct ccctgccgag agaccgctca acagcgaacg gctccgtatc agcccccatc
     3121 tgcctgaaga ttggatggct gatcatgtta tcgatcactc gaacctcatt cctgctgctg
     3181 cgtatcttga aatggctctc gagttccctg acgtcacttc agtctgggac tgccgtttcg
     3241 agtctgcttt cattctcgat ggttcggtgc ctgccgcaac cttgaaagta tctcgggacg
     3301 gaaacaggtg gagtgtcaaa tcttcaagcg ctctccaaag catgcaaggc gacctaacat
     3361 ggacccgttc tgagccgtcc ttcgactcta tccatgccta cggcaagctt ggatacggca
     3421 agccggagtt aagacctgga gctatcaccc atgttgatgt tgatgctgtg attgctcgct
     3481 cgaaagtaca agcttcgcat gaagcacttt acgatgaatt gagtcaagtt gcccaatttg
     3541 gaacagagtt ccgacggctc cagaaggcct acgccaatga tgtggacggt attgtttaca
     3601 ttaaaggtca cggtgacagc ttgactcgta ctgggtacac attccatcct gctctcatgg
     3661 atgcagtatt ccagtccggt gtctgtttta acatggtgtt cgatcatgtc aacgtcggaa
     3721 accctgagcg tacattcatg ctgccacact ccttgcgtcg cggatttagg aatgatggaa
     3781 gcacagaacc gcttatcttc aacggtgact tccgtatcta cgctatcctg gctgattggt
     3841 cgccgtactc ggcgacatat gactactatg tgctgaacga cgataatgat gtcgtcttca
     3901 cttttgaagg cctgcgtttc gaaacggtgc agcaagacca acaagcaatc aaagaacgtt
     3961 tcagcatgat ttggcaaccc cgtggcttgc ccaagtcgaa taacagtggt catgttacac
     4021 ttgaggatga ttcggttttg gaacaccaag agttgcttga tgttctagac aagctcgcct
     4081 tgggttacac caagaaggct ctagggcgcc tttctcagaa cttcgaaacg gctctgcctg
     4141 accgtaaacg ttacatggaa tgggcactcg agcgaactgc agaacgggct actgaaacct
     4201 tcttggagtt gagcgatgta tctccggtga tgaggcagaa gtatgcttct ttgttcgaac
     4261 tcacgcagcg agtcggagaa gggcagacag acattttcgt agattcaaag gctgccgtag
     4321 acatcctctt ccgagatgac ctcatgagcc gaatttacga gcatccacct ttcatcggca
     4381 gtatattcga cgaaactgtc aagcaattcg tcaagctcgt caaggatgcc atagaagccg
     4441 gcaaacgcgt tgttcgtgtg ctcgaggtcg gtgctggaac cggtaggttc acggctttgc
     4501 tcggtcaagc tcttctagat gcccagttgg aactgtgcta cgtcgactac gtttgcacgg
     4561 acatttccat ttctcttgct caggaggcca cagccaagtg tccctggatg acaattgtcc
     4621 ctgttgcatt cgacctcaac aagccacttg atcaacaaag tatcgatccc gcgagctttg
     4681 atattattgt cgcattcgat gtgctccacg cgacaccaaa cattaacgat accgttcgta
     4741 ctctccgtga gctcttactt cctggagggc atcttgcaat catcgaactg gatggaaact
     4801 cattcgccac tggtgctgtc ggaagcatct ggatggatta cgtctttggc tctttctcag
     4861 agtggtttgg tgttttggaa cagcgtccgg cctctcactg ctctctgagc aaatcagagt
     4921 ggaacagcgt cctgcattcc gaaggattcg ctgaggggct cttcctcaca tcaaccgggg
     4981 ctactgtttc tcatatggca ttcgtgtccc agaccactga gacggctcac gaacaacaaa
     5041 gctctccaac tccttctact ccctcgctgc aaagcgacca cagtcctcat cttaggtcat
     5101 tctctttctc ctcaacgccc ctccccgtta cacccaatac tcctggcttc agtggagatc
     5161 caacccgcag tgtcttgtca atcgacaacg agcatcaact gtcgaagaag ttggagggtg
     5221 gggccacttc cgctgacaag caatcgcaga tcattgctcc aggcccaatc acacttgaag
     5281 accaacgctc gtcgtctcaa tctccagagc tgtcactacc tcccaataca atcattcgtc
     5341 gattcacggc tggggatgag gtcgagttag tcagcttcgt cgctagtctc gactcctccc
     5401 ttccgttgaa gatttggctc tacactgaca cacagagcga caacgctacg ctgatcggac
     5461 tgaccagaac gctcaggcat gaattcggtt tctggaaggt gtatcttatc ctcttccatc
     5521 cttcttggag ctttgccaac caagaagagt tcattcgtaa ccagcttatc cctcttaagt
     5581 ggttggacgc ggaaatcatg gttgatgagg atggcaccat gcgagtgcca cgtattgtga
     5641 caggcgctgc cccgtcaacc acggagatgg ctgattccaa gccactgcaa ttcgatgact
     5701 cccagttttg gcgagcgctc cctgccccac tgaatgcgga tgatgtccaa gtcgctgtgt
     5761 cctttatcac actttccagt gtaattccag gtcattccga gttctctgga aaagtcacca
     5821 gtgtagggtc agaggtggaa acctctttga ttgggaagcg cgtgatgggt gtcaccaagt
     5881 cacctaaagg caactttgtg gtttgtccta aaggttgcat aacgacgatc ccggatagca
     5941 tgagccttcc cctcgctgct gctcttgccg gccgcctcgc attcacatct tcagttgtgg
     6001 ccgatacctt ggcgaatgcc aaaggtggcg ttcgtgtaat tctccacgcc gctgactgtt
     6061 ctccagctgc cgtcgcaaca tacatctatc tccaggagcg caatttcgat attttcgtga
     6121 cagcagcgaa tccgtctaca catcagattc ctcataagga agttttcgac tcaggcaatg
     6181 ttgagatttg gtcgcatgct gcacgacaat gggctgctga aggtgccgac ttcatcttca
     6241 acttcgaaga tggaactgta gtccttgagg aatcgattga gaggctagct cgtcgtggca
     6301 ccctttgcca agtcggaact ctctatcctc accgcctccg tccaggtcag cgattcgtat
     6361 ctgttgaatt tgactgtcta ttctctggcg aacataacgt tctggaagaa gccattcagt
     6421 caacttccag tcagactatt tctcgtattt ccccctcaac tctcatcttc caggcggatg
     6481 ctctccaatt ggcacactca aaagccagcc tatacaagga tctctcgatt atgttggact
     6541 tcgagacgtt accgaacaac atgactgtct ccaagcctgg tagactgcgt ggtacgaata
     6601 catttgaccc tcgtgcatcc tatgtcgtta tcggaggcat cggtggtctt ggaattgaga
     6661 ttgcaagatt gctgatcgag aagggcgccc ggcacgttat tctgacatca cgatcgggta
     6721 gcaaggcatt cgacgatggt cggcttgttc gggagaggag ggtcctcaac ttcttgcgcg
     6781 agaagcccgg ggttactatc gacctggttg ccgtggactg ccttgatgcg gcaaagacga
     6841 aaactctttt ctccaactta ccgaatgtcg ctggtgtctt ctacgtcgcc gtgagattaa
     6901 acgactcgct attcctcaat ctcactaccc aggaagactg gtcaaaagtg tacgatgtca
     6961 aggtcaaggg tcttcgcatt ctcttggatg ctgttgatcc cagaaaactc gatttccttg
     7021 tcctgacgag ctctatggca accgtcagcg gttctcccgg tcaagccaac tatgctgcag
     7081 cccagaccga aatggaggcg atgggtgcaa acattcccaa ttgcatttca gtcaccgttc
     7141 ctcctctaac cgacggtggt gtctttgtac gaagtatgcc tactggcaat gctcgtaacg
     7201 cggctctcga caaatataag gatctcggca tgactggaat gaagttggcc cgtcattgcg
     7261 tcgatgctat ctggactatt ggaacgccag cctaccaacc agtctacatt cccgcgacca
     7321 actggaagaa ggtcatggag gtcgctgctc ctgaatacct tcaatctagt atgagacact
     7381 tgttggtcaa agagaccact gacagtgtag cagccgacgg cggcaaagaa caaaccattc
     7441 ttggagcctg tgcggctgtg ctatcgcttg acattgaccg cgtcgaagat aatattcctc
     7501 tcgctagcta cggcttggat tcattgacgt cggttcgcct cagtggtgtt ctcaaggctc
     7561 gtttcggggt tgaagtcaca cagctgcagt tgttgagtca gactatgaca gttgcgcgct
     7621 tatgtcagct ccaggaggaa caacaagcat ctgtcaccac aaccgccacc gccaacgatg
     7681 accgcaccca agtcgatccg gccaaggtgc atgaagcgga tatggatcaa actattgtgc
     7741 ggctcaacac tccaaccgaa ggcactcctc tcttcctcat tcacggtgct ggtggaggta
     7801 tcgtcgtact tgtcaaagca gcccagaggg ttcactatcc tgtctatggt gtgcaggata
     7861 ctccagaggc acccatcacc ggtaatctcc gccgattgtc agaattctac ttgatgaaga
     7921 tccgggagaa acagccacac ggtccttaca gacttggagg attctcattc ggaacgtgct
     7981 tggctttgga catttccctt ttactgcaac aacagggcga ggttgtcgaa tcattggtgc
     8041 tcttggatgg ttcgcctact ctcttcaagc gacctgcatt ccagtcatat ctcatgggac
     8101 gcatccaaga cggcagtttc caggaagaca tcctcgatgt cgtcaacgac atggcgtcta
     8161 gtggaacgct cgatgacgcg gaggatttag gccgtcagtt tgctgaccac ttccagcgtg
     8221 ccaaggaagg tggaagtgga ccaaaatggg tcgctcgctt ctgcactgcc tatgtcgctc
     8281 acctcattat gggtcttcgt tgtggtaaag agttcaacgc tttagaggcg aaatacggaa
     8341 aggatgcgat gggtgcttgg ccagcaaaga ggaccgtgct cgtgcgtgct ctcagcggtg
     8401 tatctgctga accttatgca gccggtgctt cggagtactt tgacttggac gtctatggct
     8461 caggcgtgga gaagcatgac ctgcctggca cgcacttcgg catccttgca cccaaatccg
     8521 gactcgacgc cattctcgac aaactgcact aaggagattg ataagacttt tctagttgca
     8581 tatcttttat atttaaatct tatctattag ttaatttttt gtaatttatc cttatatata
     8641 gtctggttat tctaaaatat catttcagta tctaaaaatt cccctctttt ttcagttata
     8701 tcttaacagg cgacagtcca aatgttgatt tatcccagtc cgattcatca gggttgtgaa
     8761 gcattttgtc aatggtcgaa atcacatcag taatagtgcc tcttacttgc ctcatagaat
     8821 ttctttctct taacgtcacc gtttggtctt ttatagtttc gaaatctatg gtgataccaa
     8881 atggtgttcc caattcatcg ttacgggcgt attttttacc aattgaagta ttggaatcgt
     8941 caattttaaa gtatatctct cttttacgta aagcctgcga gatcctctta agtatagcgg
     9001 ggaagccatc gttattcgat attgtcgtaa caaatacttt gatcggcgct atgcggccgc
     9061 caccgcggtg gagctccagc ttttgttccc tttagtgagg gttaattgcg cgcttggcga
     9121 gactcaaaca agatatattt tgcccgcctt acagacgaaa ctagttggag gttatggagc
     9181 atactatcac gtgggcggcc actggtgagt tactacaccc caggggcaac gttgatgctc
     9241 ctaaaaaact ctggctggac gcaagccgta acacccgtgt cacttcataa tcgtttgcaa
     9301 ttcagggctt gatctacaca ttaccctgtt atccctatta attaatacgg ttatccacag
     9361 aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc
     9421 gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca
     9481 aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt
     9541 ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc
     9601 tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc
     9661 tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc
     9721 ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact
     9781 tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg
     9841 ctacagagtt cttgaagtgg tggcctaact acggctacac tagaagaaca gtatttggta
     9901 tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca
     9961 aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa
    10021 aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg
    10081 aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc
    10141 ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa acttggtctg
    10201 acagttacca atgcttaatc agtgaggcac ctatctcagc gatctgtcta tttcgttcat
    10261 ccatagttgc ctgactcccc gtcgtgtaga taactacgat acgggagggc ttaccatctg
    10321 gccccagtgc tgcaatgata ccgcgagacc cacgctcacc ggctccagat ttatcagcaa
    10381 taaaccagcc agccggaagg gccgagcgca gaagtggtcc tgcaacttta tccgcctcca
    10441 tccagtctat taattgttgc cgggaagcta gagtaagtag ttcgccagtt aatagtttgc
    10501 gcaacgttgt tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt
    10561 cattcagctc cggttcccaa cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa
    10621 aagcggttag ctccttcggt cctccgatcg ttgtcagaag taagttggcc gcagtgttat
    10681 cactcatggt tatggcagca ctgcataatt ctcttactgt catgccatcc gtaagatgct
    10741 tttctgtgac tggtgagtac tcaaccaagt cattctgaga atagtgtatg cggcgaccga
    10801 gttgctcttg cccggcgtca atacgggata ataccgcgcc acatagcaga actttaaaag
    10861 tgctcatcat tggaaaacgt tcttcggggc gaaaactctc aaggatctta ccgctgttga
    10921 gatccagttc gatgtaaccc actcgtgcac ccaactgatc ttcagcatct tttactttca
    10981 ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg
    11041 cgacacggaa atgttgaata ctcatactct tcctttttca atattattga agcatttatc
    11101 agggttattg tctcatgagc ggatacatat ttgaatgtat ttagaaaaat aaacaaatag
    11161 gggttccgcg cacatttccc cgaaaagtgc cacctgaacg aagcatctgt gcttcatttt
    11221 gtagaacaaa aatgcaacgc gagagcgcta atttttcaaa caaagaatct gagctgcatt
    11281 tttacagaac agaaatgcaa cgcgaaagcg ctattttacc aacgaagaat ctgtgcttca
    11341 tttttgtaaa acaaaaatgc aacgcgagag cgctaatttt tcaaacaaag aatctgagct
    11401 gcatttttac agaacagaaa tgcaacgcga gagcgctatt ttaccaacaa agaatctata
    11461 cttctttttt gttctacaaa aatgcatccc gagagcgcta tttttctaac aaagcatctt
    11521 agattacttt ttttctcctt tgtgcgctct ataatgcagt ctcttgataa ctttttgcac
    11581 tgtaggtccg ttaaggttag aagaaggcta ctttggtgtc tattttctct tccataaaaa
    11641 aagcctgact ccacttcccg cgtttactga ttactagcga agctgcgggt gcattttttc
    11701 aagataaagg catccccgat tatattctat accgatgtgg attgcgcata ctttgtgaac
    11761 agaaagtgat agcgttgatg attcttcatt ggtcagaaaa ttatgaacgg tttcttctat
    11821 tttgtctcta tatactacgt ataggaaatg tttacatttt cgtattgttt tcgattcact
    11881 ctatgaatag ttcttactac aatttttttg tctaaagagt aatactagag ataaacataa
    11941 aaaatgtaga ggtcgagttt agatgcaagt tcaaggagcg aaaggtggat gggtaggtta
    12001 tatagggata tagcacagag atatatagca aagagatact tttgagcaat gtttgtggaa
    12061 gcggtattcg caatatttta gtagctcgtt acagtccggt gcgtttttgg ttttttgaaa
    12121 gtgcgtcttc agagcgcttt tggttttcaa aagcgctctg aagttcctat actttctaga
    12181 gaataggaac ttcggaatag gaacttcaaa gcgtttccga aaacgagcgc ttccgaaaat
    12241 gcaacgcgag ctgcgcacat acagctcact gttcacgtcg cacctatatc tgcgtgttgc
    12301 ctgtatatat atatacatga gaagaacggc atagtgcgtg tttatgctta aatgcgtact
    12361 tatatgcgtc tatttatgta ggatgaaagg tagtctagta cctcctgtga tattatccca
    12421 ttccatgcgg ggtatcgtat gcttccttca gcactaccct ttagctgttc tatatgctgc
    12481 cactcctcaa ttggattagt ctcatccttc aatgctatca tttcctttga tattggatca
    12541 tactaagaaa ccattattat catgacatta acctataaaa ataggcgtat cacgaggccc
    12601 tttcgtctcg cgcgtttcgg tgatgacggt gagcgcgttt cggtgatgac ggtgaaaacc
    12661 tcggcgcgcc attaccctgt tatccctaat atacaacgca aagaagctgg aaaattggcg
    12721 agggaatcct gtttctgtct atccaagaat gggcatgagg tggcaaccgt cgtgctagcg
    12781 tacagggtgc actttgtaac catttgggac accggacact cgctgttttc gaaattaccc
    12841 tttaagcgcg ggtattgaac caggcttatg cccaagatcg tagcaagcat ttaactgtgg
    12901 gaatactcag gtatcgtaag atgcaagagt tcgaatctct tagcaaccat tatttttttc
    12961 ctcaacataa cgagaacaca caggggcgct atcgcacaga atcaaattcg atgactggaa
    13021 attttttgtt aatttcagag gtcgcctgac gcatatacct ttttcaactg aaaaattggg
    13081 agaaaaagga aaggtgagag cgccggaacc ggcttttcat atagaataga gaagcgttca
    13141 tgactaaatg cttgcatcac aatacttgaa gttgacaata ttatttaagg acctattgtt
    13201 ttttccaata ggtggttagc aatcgtctta ctttctaact tttcttacct tttacatttc
    13261 agcaatatat atatatatat ttcaaggata taccattcta atgtctgccc ctaagaagat
    13321 cgtcgttttg ccaggtgacc acgttggtca agaaatcaca gccgaagcca ttaaggttct
    13381 taaagctatt tctgatgttc gttccaatgt caagttcgat ttcgaaaatc atttaattgg
    13441 tggtgctgct atcgatgcta caggtgttcc acttccagat gaggcgctgg aagcctccaa
    13501 gaaggctgat gccgttttgt taggtgctgt gggtggtcct aaatggggta ccggtagtgt
    13561 tagacctgaa caaggtttac taaaaatccg taaagaactt caattgtacg ccaacttaag
    13621 accatgtaac tttgcatccg actctctttt agacttatct ccaatcaagc cacaatttgc
    13681 taaaggtact gacttcgttg ttgtcagaga attagtggga ggtatttact ttggtaagag
    13741 aaaggaagac gatggtgatg gtgtcgcttg ggatagtgaa caatacaccg ttccagaagt
    13801 gcaaagaatc acaagaatgg ccgctttcat ggccctacaa catgagccac cattgcctat
    13861 ttggtccttg gataaagcta atgttttggc ctcttcaaga ttatggagaa aaactgtgga
    13921 ggaaaccatc aagaacgaat tccctacatt gaaggttcaa catcaattga ttgattctgc
    13981 cgccatgatc ctagttaaga acccaaccca cctaaatggt attataatca ccagcaacat
    14041 gtttggtgat atcatctccg atgaagcctc cgttatccca ggttccttgg gtttgttgcc
    14101 atctgcgtcc ttggcctctt tgccagacaa gaacaccgca tttggtttgt acgaaccatg
    14161 ccacggttct gctccagatt tgccaaagaa taaggtcaac cctatcgcca ctatcttgtc
    14221 tgctgcaatg atgttgaaat tgtcattgaa cttgcctgaa gaaggtaagg ccattgaaga
    14281 tgcagttaaa aaggttttgg atgcaggtat cagaactggt gatttaggtg gttccaacag
    14341 taccaccgaa gtcggtgatg ctgtcgccga agaagttaag aaaatccttg cttaaaaaga
    14401 ttctcttttt ttatgatatt tgtacataaa ctttataaat gaaattcata atagaaacga
    14461 cacgaaatta caaaatggaa tatgttcata gggtagacga aactatatac gcaatctaca
    14521 tacatttatc aagaaggaga aaaaggagga tgtaaaggaa tacaggtaag caaattgata
    14581 ctaatggctc aacgaaatga cggtatcgat aagcttgata tcgaattcct gcagcccggg
    14641 ggatccacta gttctagatt aattaatatc taaaaattgc cttatgatcc gtctctccgg
    14701 ttacagcctg tgtaactgat taatcctgcc tttctaatca ccattctaat gttttaatta
    14761 agggattttg tcttcattaa cggctttcgc tcataaaaat gttatgacgt tttgcccgca
    14821 ggcgggaaac catccacttc acgagactga tctcctctgc cggaacaccg ggcatctcca
    14881 acttataagt tggagaaata agaga
//
