LOCUS       sequence-212575-       17545 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of a fungal biosynthetic gene cluster.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 17545)
  AUTHORS   Harvey CJB, Tang M, Schlecht U, Horecka J, Fischer CR, Lin HC, Li J,
            Naughton B, Cherry J, Miranda M, Li YF, Chu AM, Hennessy JR, Vandova
            GA, Inglis D, Aiyar RS, Steinmetz LM, Davis RW, Medema MH, Sattely 
            E, Khosla C, St Onge RP, Tang Y, Hillenmeyer ME
  TITLE     HEx: A heterologous expression platform for the discovery of fungal 
            natural products.
  JOURNAL   Sci Adv. 2018 Apr 11;4(4):eaar5459. doi: 10.1126/sciadv.aar5459. 
            eCollection 2018 Apr.
  PUBMED    29651464
REFERENCE   2  (bases 1 to 17545)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Adv."; 
            date: "2018-04-11"; volume: "4(4):eaar5459. doi"; pages: " 
            10.1126/sciadv.aar5459. eCollection 2018 Apr"
COMMENT     Sequence Label: pCHKU27.2-2.2
FEATURES             Location/Qualifiers
     source          1..17545
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(1..17)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1..17)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     terminator      1297..1596
                     /label=ADH2 terminator
                     /note="transcription terminator for the S. cerevisiae 
                     alcohol dehydrogenase 2 (ADH2) gene"
     promoter        4223..4443
                     /gene="S. cerevisiae URA3"
                     /label=URA3 promoter
     CDS             4444..5247
                     /codon_start=1
                     /gene="S. cerevisiae URA3"
                     /product="orotidine-5'-phosphate decarboxylase, required 
                     for uracil biosynthesis"
                     /label=URA3
                     /note="yeast auxotrophic marker, counterselectable with 
                     5-fluoroorotic acid (5-FOA)"
                     /translation="MSKATYKERAATHPSPVAAKLFNIMHEKQTNLCASLDVRTTKELL
                     ELVEALGPKICLLKTHVDILTDFSMEGTVKPLKALSAKYNFLLFEDRKFADIGNTVKLQ
                     YSAGVYRIAEWADITNAHGVVGPGIVSGLKQAAEEVTKEPRGLLMLAELSCKGSLSTGE
                     YTKGTVDIAKSDKDFVIGFIAQRDMGGRDEGYDWLIMTPGVGLDDKGDALGQQYRTVDD
                     VVSTGSDIIIVGRGLFAKGRDAKVEGERYRKAGWEAYLRRCGQQN"
     primer_bind     complement(13695..13715)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(13695..13713)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     rep_origin      complement(14052..14640)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(14132..14151)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(14811..15602,15603..15671))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     15434..15453
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(15672..15776)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      15803..17145
                     /label=2u ori
                     /note="yeast 2u plasmid origin of replication"
     protein_bind    complement(16766..16813)
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     primer_bind     17186..17204
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 gatccactag ttctagatta attaatatct aaaaattgcc ttatgatccg tctctccggt
       61 tacagcctgt gtaactgatt aatcctgcct ttctaatcac cattctaatg ttttaattaa
      121 gggattttgt cttcattaac ggctttcgct cataaaaatg ttatgacgtt ttgcccgcag
      181 gcgggaaacc atccacttca cgagactgat ctcctctgcc ggaacaccgg gcatctccaa
      241 cttataagtt ggagaaataa gagaatttca gattgagaga atgaaaaaaa aaaaaaaaaa
      301 aaaggcagag gagagcatag aaatggggtt cactttttgg taaagctata gcatgcctat
      361 cacatataaa tagagtgcca gtagcgactt ttttcacact cgaaatactc ttactactgc
      421 tctcttgttg tttttatcac ttcttgtttc ttcttggtaa atagaatatc aagctacaaa
      481 aagcatacaa tcaactatca actattaact atatcgtaat acacaatgac aatcctctgt
      541 ctacatggcg gcttcggcag cgcccaaaac ttccaagtgc aactccatcc cctagttagt
      601 acctaccaat cattataccc cgataccacc ttccactaca ttaatggcgg tcatcccgcc
      661 acgcctccac caggaagcga ggattacttc ggcccaccgc ctcattaccg attcatcgaa
      721 tacgatggga ttgggcggtc cgacgatgtg atggagcgga ttcgtcagct cccgagggga
      781 gccacggccg aagataccat gcgggtgttg acccacgaac atgagatgat gtctgccgat
      841 tgtgtgagag aggcgttgca gagactgttg gggatattgg atgaacatcc ggaaattgac
      901 ggagtcttgg gcttctcgga gggcgctaca gtcgctgcta ctttgttgct tgaagaggag
      961 cgacttgttc aggaggaggg taggaagaga cagcttaagt acggggtctt catagcaggc
     1021 tggcctcccc ttcgtattga cggcgatcga gtaacaggct gtctggcgga tgagacagag
     1081 gatatgatcg aagtgccgac gtgtcatatc atcggggcga atgaccccta cgtggatggc
     1141 accatggcgc tgtatggggt ttgtgatccc gatacggcca tcatgtttga tcatggcaag
     1201 ggccatacca ttccgcggga cgtggcgacg atgaaggagg cggcagaggt gattgagcag
     1261 accaggagac gggggattgc tgccggggat gcgtaggcgg atctcttatg tctttacgat
     1321 ttatagtttt cattatcaag tatgcctata ttagtatata gcatctttag atgacagtgt
     1381 tcgaagtttc acgaataaaa gataatattc tactttttgc tcccaccgcg tttgctagca
     1441 cgagtgaaca ccatccctcg cctgtgagtt gtacccattc ctctaaactg tagacatggt
     1501 agcttcagca gtgttcgtta tgtacggcat cctccaacaa acagtcggtt atagtttgtc
     1561 ctgctcctct gaatcgtctc cctcgatatt tctcattttc cttcgcatgc cagcattgaa
     1621 atgatcgaag ttcaatgatg aaacggtaat tcttctgtca tttactcatc tcatctcatc
     1681 aagttatata attctatacg gatgtaattt ttcacttttc gtcttgacgt ccaccctata
     1741 atttcaatta ttgaaccctc acgatccagt tctccagtga cacagccttt atctggtcaa
     1801 acctttcttt ctaatcacct atgctgatgc ttaattaagg gatttttgtc tccatcaacg
     1861 gcatgcgccc aaaaatgacg ttttttttaa cccatagaca cgaaactacc cattttccac
     1921 cggcctgacc taccaccgga acaacggcca tctccaactt gcaagttggg gaaattaaga
     1981 gcatcgcagg tttaatggaa gaaaaaaaaa aggtacagca cagcgcaaat ggagttagtt
     2041 cccttatgtc acacactcac acacagtcgg tcagatcaag catactgggt gcgtataaat
     2101 agagtggcca ttgccaccct gtttatctca aaatctgtct tgttagtggt cttctccctt
     2161 tttcaggtta caattctctt gtttctactt agtatataag tatatcaagc tatattaagc
     2221 atactatcaa ctgtcaactc tatcctcaaa atacaataca aaatgcagct ctcattgttg
     2281 gctcttcttg gagcattggc gctgcccagc gctgctgttc cccatgcgcg cgatgaggga
     2341 caatgcaagc agaccactgt tgctattctt ggtggaggaa tggctggtat tgctgctgct
     2401 caaacgctgc acaatgcctc catggatgac ttcatgatcc tcgaataccg cgacaccatc
     2461 ggcggtcgcg cctggcataa gcccttcggt caggacaaag atgggaaccc atacatcatt
     2521 gagatgggtt gtaactgggt ccaaggactc ggcactcctg ggggtccgca gaaccccgtc
     2581 tggacactgg cccaagtcta caacctctcc accatctact ccaactacag caacgtgtcg
     2641 acctacaacc agcacggcta caaagactac tcccacctga tcgacacctg ggatgacatc
     2701 tacgacaccg ccgccgccca agccggcgtc atgctcctcg acaacctgca ggaccagaca
     2761 gcccagaccg gactggccct cgccggctgg cgccccaagg tcgacgacat ggaagcacaa
     2821 gccgttgact ggtggtcatg ggacttcgaa gacgcctaca cgcccctcga aagttccttt
     2881 atcttcggcg tcgcaggcca aaacctcacc gtgaacggct tcagcgacga agacaacttt
     2941 gtcatcgacc aacgcggcta cagccacatc atccacggca tggcgtcgac cttcctcaag
     3001 cccaacgaca ctcgcctgct cctcaacaac cacatcacca acatcagcta ctccgactcc
     3061 ggtgtgaccg tccacagttc cgacggctcc tgcgtgcgcg cctcctacgc catctgcacc
     3121 ttcagtctgg gcgtattgca acacgacgcc gtcaccttca ccccgtccct gcccgaatgg
     3181 aagaaagaag ccatcgaagg cttcacaatg gccacctaca ccaagatctt cctccagttc
     3241 aacgagacct tctggcccga agacacccaa tacttcctct acgcggaccc ctacatgcgc
     3301 ggctactacc ccgtcttcca gtccctcagc accgagggct tcttcccggg ctccaacatc
     3361 atcttcgtca ccgtgacgga gcaattcgcc tggcgcgccg aacgccagtc cgacgagaag
     3421 accaaagcag aggtgatgga agtcctgcgc aagatgttcc cggagaagga tatccctgac
     3481 ccgattgcgt tcatgtaccc gcggtggacc ctggagccat gggcttacgg cagttactcg
     3541 aactggccgc ccagcacgac gttggagatg catgagaacc tgcgtgccaa tgcggggaga
     3601 ctgtggtttg ccggtgaggc aaccagtcca acctactttg ggttcttgca tggagcttgg
     3661 ttcgagggcc aggctgcggg gcttcatctt agcagtatct tgaacggcac ttgcaaggcg
     3721 aatactactt gcaagggcag gaagcattac gagacgttgc acggcacgac gccattgtcg
     3781 gattacagct tcgtggatgg atggaacgga aacagtttct atgattttaa cgatgattaa
     3841 acaaatcgct cttaaatata tacctaaaga acattaaagc tatattataa gcaaagatac
     3901 gtaaattttg cttatattat tatacacata tcatatttct atatttttaa gatttggtta
     3961 tataatgtac gtaatgcaaa ggaaataaat tttatacatt attgaacagc gtccaagtaa
     4021 ctacattatg tgcactaata gtttagcgtc gtgaagactt tattgtgtcg cgaaaagtaa
     4081 aaattttaaa aattagagca ccttgaactt gcgaaaaagg ttctcatcaa ctgtttaaaa
     4141 ggaggatatc aggtcctatt tctgacaaac aatatacaaa tttagtttca aagatgaatc
     4201 agtgcgcgaa ggacataact cattcaattc aattcatcat ttttttttta ttcttttttt
     4261 tgatttcggt ttccttgaaa tttttttgat tcggtaatct ccgaacagaa ggaagaacga
     4321 aggaaggagc acagacttag attggtatat atacgcatat gtagtgttga agaaacatga
     4381 aattgcccag tattcttaac ccaactgcac agaacaaaaa cctgcaggaa acgaagataa
     4441 atcatgtcga aagctacata taaggaacgt gctgctactc atcctagtcc tgttgctgcc
     4501 aagctattta atatcatgca cgaaaagcaa acaaacttgt gtgcttcatt ggatgttcgt
     4561 accaccaagg aattactgga gttagttgaa gcattaggtc ccaaaatttg tttactaaaa
     4621 acacatgtgg atatcttgac tgatttttcc atggagggca cagttaagcc gctaaaggca
     4681 ttatccgcca agtacaattt tttactcttc gaagacagaa aatttgctga cattggtaat
     4741 acagtcaaat tgcagtactc tgcgggtgta tacagaatag cagaatgggc agacattacg
     4801 aatgcacacg gtgtggtggg cccaggtatt gttagcggtt tgaagcaggc ggcagaagaa
     4861 gtaacaaagg aacctagagg ccttttgatg ttagcagaat tgtcatgcaa gggctcccta
     4921 tctactggag aatatactaa gggtactgtt gacattgcga agagcgacaa agattttgtt
     4981 atcggcttta ttgctcaaag agacatgggt ggaagagatg aaggttacga ttggttgatt
     5041 atgacacccg gtgtgggttt agatgacaag ggagacgcat tgggtcaaca gtatagaacc
     5101 gtggatgatg tggtctctac aggatctgac attattattg ttggaagagg actatttgca
     5161 aagggaaggg atgctaaggt agagggtgaa cgttacagaa aagcaggctg ggaagcatat
     5221 ttgagaagat gcggccagca aaactaaaaa actgtattat aagtaaatgc atgtatacta
     5281 aactcacaaa ttagagcttc aatttaatta tatcagttat taccctatgc ggtgtgaaat
     5341 accgcacaga tgcgtaagga gaaaataccg catcaggata ggaaaaaacc gagcttcctt
     5401 tcatccggcg cggctgtgtt ctacatatca ctgaagctcc gggtatttta agttatacaa
     5461 gggaaagatg ccggctagac tagcaagttt taggctgctt aacattatgg ataggcggat
     5521 aaagggccca aacaggattg taaagcttag acgcttctgg ttggacaatg gtacgtttgt
     5581 gtattaagta aggcttggct ggggatagca acattgggca gagtatagaa gaccacaaaa
     5641 aaaaggtata taagggcaga gaagtctttg taatgtgtgt aacttctctt ccatgtgtaa
     5701 tcagtatttc tacttacttc ttaaatatac agaagtaaga cagataacca acagcctttc
     5761 ccagatatac atatatatct ttatttcagc ttaaacaata attatatttg tttaactcaa
     5821 aaataaaaaa aaaaaaccaa actcacgcaa ctaattattc cataataaaa taacaacatg
     5881 gctaccaccc ccggacaacg tctcttccct gtggtcgtcg accacctcgc ccagaccaac
     5941 ccagctcagc gcatcggact catccccaag ggtgctgatg tctctgatgg ctatgatact
     6001 gtgaccgtcc agcgtctggc ccgcgcggtg aatgtctttg cttggtggat tgagaagcag
     6061 atcggacgcg cccagaagcc agagacaatt gcttacatgg ctagcaatga tgtgcgatac
     6121 ttgattttcc tcttggccgc tcataagacg ggatacaagc ccctcctccc ctctactcgt
     6181 ctttccgacg acgcctacca gcacatcctc aacgccaccg actgccacgc cttcttctac
     6241 acccccgaaa agcagcgccg cgtgtctgaa attcaggcct tccgtccgga tactgccttc
     6301 catgaggtgc cctctatcgc agagcttttc agcaccactg ctcagcccta cccctacacc
     6361 aagacctacc aggaagcgga ggacgatgtc gccatcatca tccacagctc cggcacaaca
     6421 ggcatgccta aacccgtccc cctgacgcac ggcttcctcg ccaccctcga ctacggcgca
     6481 tacatttcgc gtccccaatc ccgccaatcc accatgttca acgacctcca gcccgacacc
     6541 ctcgtcctct ccaccacccc cttcttccac cttatgggtc tgctcgcctt cgccgaatcc
     6601 ctcttccacc acatcccctt tgtcgttctc cccgacaagc ctctctccgc cgacctcctc
     6661 acctccgtca tcaccgccac caagcccgcg gccgccatgc tgcccccttc ccttctcgaa
     6721 gacatgtccc acgtcccagc tgcccgcgcc gcactcagca ccctcgaaaa tgtctacttc
     6781 gctggcgccc ctctctcccc ggaagtcggc gatgatctgt cccagctgac caagatcatc
     6841 actgtcatcg gatccagcga aatgggcctg atctcctcgt tcgtgcccca aggcacaaat
     6901 gtgtggggct acttcgactg gaaccccgcc tacggagtag acatgcgaca tgtcagcgac
     6961 gatatgtatg agcttatcat cccccgccgc gcaaacagtc gccgaattca tggcatcttc
     7021 cacaccttcc cagaccgctc cgagtaccgc tccaacgacc tcttcacccg acaccccgag
     7081 caccctcacc tgtggaaata ccacggccga cttgacgacg tgatcgttct cagcaacggc
     7141 gagaaactga accccgtcac cctggagaag atcgtcgagg ggcacccgcg cgtgcagcgg
     7201 gctctgctca tcggccaagc ccgcttcgac acggccctca tcatcgaacc caacagggct
     7261 ctcgagggcg agaacatcga cgaaaaagcc tttatcgaga gcatttggcc caccgtggag
     7321 cgcgcgaacg aagccgtccc caaatacggc cgcatcgcaa agaccaggat ccgtctctct
     7381 gaccccaaga agcccttcca actgaccccc aagggcacca cgaagcgcca cgccgtgaac
     7441 aaggactaca aagacgagat cgacgccatc tacgcagcag ccgaggaaga atcagccgcc
     7501 gtcgagctcc ctaccaccct caccaccgac aacctccgca ctttcgtccg ctccctcatc
     7561 gccaacctcc tcgaccgcac tgacctccgc gacgacgagg acctctacgc cgccggcctg
     7621 gactcgctgc aaaccatcca gctcgccaaa acccttaaca aggccgtcgt cgccgccggc
     7681 taccccgccg gtcacgacat ctcccagcag caaatctatg ccaacccctc cgtatcccag
     7741 ctcgctgcgt acctacacga cctagtgacc ggcaacctgc gcacaacgac catctcccgc
     7801 gccgaccgca tcgccgccac catcgacaaa tacaccacca agctcccgcg ccgcaccacg
     7861 cccgccgtcc ctgccccggc caactccacc gtgatcctca ccggctcgac cgggtcgctg
     7921 ggaacctacc tcctacacag cctgctcacc gacccaaaga tcgcaaaagt ctactgcttc
     7981 aaccggtccg atgcggccga gcgccaacag cgcagcttcg ccgacaaggg tctggatact
     8041 gcgctgctca accccagccg ggtggaattc ctcactgtgt catttggcgc gccccgcttc
     8101 ggcctgagtg agacaaagta cgccgatctc ctggagactg tggacttcat tatccacaac
     8161 gcatggaaag tcaacttcaa tcacccgctg gagtcgttcg aggacccgca tatcaagggc
     8221 gtggtggagt ttatttcctt cagtgtgagg agcaagtaca acgcacactt gtcgttcgtg
     8281 tccagtgtga gtaccatcgg tggatggacg gagaagttgg gccccgtggt gccggagacg
     8341 ccggtggaga gtgtcgatgc ggtgctggtg caggggtacg gcgagtcgaa gttcgtcggg
     8401 gagaggttgt gtctggctgc gtcggagggg atgggtgtgc ccacgacggt gtttagggtt
     8461 gggcagattt ccgggccgac gactgagaag ggcatgtgga atttggatga gtgggtgcct
     8521 acgttggtga agaccagtaa ggcggttgga aaggtgccgg cggatttggg tgggtatgag
     8581 gttgattggg tgcctgttga taccctctcc accatcacca ccgaactcat gcacacccgt
     8641 cgccaaaccc aatccgctac tccacacgcc gtctaccacc ttgtcaaccc ctgcaagact
     8701 ccctgggcga ccctcgtccc cgccgttcag gccaaatacc ccggtatgca gacggtgccg
     8761 ctagaccagt ggctggacga gctggaggcg atcaagtcac cgtcggaaac cgaggtgcgc
     8821 gagaagcccg cgctgaaact gcttgatttc taccgcggac tcgcgggcga ggtgctctcg
     8881 gccagtatta gtgtggagca gacgcgcggg ggcagcaaga ccatggaggg attgggggct
     8941 gtgacaggcc agttgatggg gaattggttg gggcagtggg atttctgaag tgcttttaac
     9001 taagaattat tagtcttttc tgcttatttt ttcatcatag tttagaacac tttatattaa
     9061 cgaatagttt atgaatctat ttaggtttaa aaattgatac agttttataa gttacttttt
     9121 caaagactcg tgctgtctat tgcataatgc actggaagga gaaaaaaaag gtgcacacgc
     9181 gtggcttttt cttgaatttg cagtttgaaa aataactaca tggatgataa gaaaacatgg
     9241 agtacagtca ctttgagaac cttcaatcag ctggtaacgt cttcgttaat tggatactca
     9301 aaaaagatgg atagcatgaa tcacaagatg gaaggaaatg cgggccacga ccacagtgat
     9361 atgcatatgg gagatggaga tgatacctcc attgggccga tgaagttagt cgacggatag
     9421 aagcggttgt cccctttccc ggcgagccgg cagtcgggcc gaggttcgga taaattttgt
     9481 attgtgtttt gattctgtca tgagtattac ttatgttctc tttaggtaac cccaggttaa
     9541 tcaatcacag tttcataccg gctagtattc aaattatgac ttttcttctg cagtgtcagc
     9601 cttacgacga ttatctatga gctttgaata tagtttgccg tgattcgtat ctttaattgg
     9661 ataataaaat gcgaaggatc gatgaccctt attattattt ttctacactg gctaccgatt
     9721 taactcatct tcttgaaagt atataagtaa cagtaaaata taccgtactt ctgctaatgt
     9781 tatttgtccc ttatttttct tttcttgtct tatgctatag tacctaagaa taacgactat
     9841 tgttttgaac taaacaaagt agtaaaagca cataaaagaa ttaagaaaat gcggtcactg
     9901 caggttttcg ccctggcagg cgtccttggc gcgtctgccc ttgatgccga ctctatcctg
     9961 agcaaatact ttggcaacga tgctccttgg tatagcgatc gcattcccct cttcgagaca
    10021 gatctctccg acattcaaga tgtctactat taccgctgga gcattttccg ggctcaccag
    10081 cgtgaccttg gctcctacgg ctatatctcc accgagttcc tcaacgacgt cagctggcag
    10141 gaagaacctt atgccctgct gattgatgcc tccaacttcc atctgcgcga aggccgctgg
    10201 tgccgcgacc gtcgcttcac cagcgactac tcgtcttttc tcttcggccc ccatggcgcc
    10261 ccctaccaat tctccgaatc catggccgac ggcgtctggc aggcctacct ggtcaacggc
    10321 gtcgctgacg atgccactcc cctgctgtcc tccatgcaga ccgtctacgc cggctggaac
    10381 tccacctctc tcggtgtcgg tggctacgac acctccaagg gcctctactg gatccagcct
    10441 ctgactgatg cgaccgaata caccattgcc agcatcgacg cctccggagg cgaagacggc
    10501 ttcaccggcg gatatgcctt ccgtcccagc atcaacagct accaatatgc taatgccctg
    10561 gctattgccc acatcgccaa cctcaccggc gacactaccg tccgcgagca atacacccag
    10621 ctcgcaagcg ccctcaagac ccgtctccag gacgacctct ggaacagcac cttcgagcac
    10681 ttcatcgacc ggtacaagga agacaacgac tacgtgacct actgggactt catccgcggc
    10741 cgcgaacttg tcggctacgt cccttggaca catgacctcc cggacgacaa caccacctac
    10801 gccaacgcat ggactcacat cctcaactcc tcacagctag caggcgagca cggccttcgc
    10861 accaacgaac ccagctacca atactacatg gtccagtacc gctatgacgg ctccaacccg
    10921 gagtgccagt ggaacggccc cgcctggccc tatcaaacca cgcaagtcct caccggcctc
    10981 gccaacctcc tggaccacta ccccaaaacc gccgcaacag gcgtcatcac caccacagac
    11041 tacaccaaca tcctcctcca gtacgccaag ctacactaca accccgcccg cggcggtgtc
    11101 cttgacatcg aagaggacta ctactctgac acgggcagcc ctatcgtcgg cctgccccgc
    11161 tccccgcact acttccactc gggcttcgtc gacctcattc tctcgggcta cgtgggcatc
    11221 cgacctcgcg cggacaatgt cctggaagtt aacccccaag cggacgcttc ggcagtgagc
    11281 tacttccgct ccgagcgcat catctaccac ggccacgagg tcgccgtgca atgggacgcc
    11341 acaggcagtc gatacggcac cgtgggcctt ctcgtcgaag tcgacggcga aaccgtggcc
    11401 accagcaaga ccctcacccg tctcacggtt gagttaagct ccgcttccgc cccggcagtc
    11461 tcccgtccca tcgccgtctc ggtgcaactc gccgctgata gcgagtatcc gcggggtagt
    11521 gtatctgtgg cagacgccga cacggacgcc gtgcactcag ccatcgacgg ccgggtctgg
    11581 ttctattgga tcgaggacgt agccaatggg tgggatagtc ccgttgggaa tgggacggag
    11641 gtgtggtacc aggtggaatt tgagaatgcg accagcacag caagtgcgga gattgcgttc
    11701 tttgcgaata gtacgcaggg gtatgatgtg ccggggagct atcgggtgga aacgagcgat
    11761 tcgacgaccg gggagtggaa ggaggtgagc aatgggaatt atagctcggc ggtggcgaat
    11821 ggaataacga cggtgagctg ggatgaggtg acgacggagg cgttgaggtt ggtgttcacg
    11881 cctaagacgg gggagatggt gcggttggtg gagtggaagg tgtttgggta gatttaactc
    11941 cttaagttac tttaatgatt tagtttttat tattaataat tcatgctcat gacatctcat
    12001 atacacgttt ataaaactta aatagattga aaatgtatta aagattcctc agggattcga
    12061 tttttttgga agtttttgtt tttttttcct tgagatgctg tagtatttgg gaacaattat
    12121 acaatcgaaa gatatatgct tacattcgac cgttttagcc gtgatcatta tcctatagta
    12181 acataacctg aagcataact gacactacta tcatcaatac ttgtcacatg agaactctgt
    12241 gaataattag gccactgaaa tttgatgcct gaaggaccgg catcacggat tttcgataaa
    12301 gcacttagta tcacactaat tggcttttcg ctagtcttat ctaaaaattg cctttatagt
    12361 ccgtctctcc agtcacggcc tgtgtaactg attaatcctg cctttctaat caccattcta
    12421 ctgtttaatt aagggatttt gtcttcatca acggcttccg cccaaaaaaa agtatgacgt
    12481 tttgcccgca ggcgtgaagc tgcccatctt cacgggcctg acctcctctg ccggaacacc
    12541 ggccatctcc aactcataaa ttggagaaat aagagaattt cagattttca gaggatgaaa
    12601 aaaaaaaggt agagagcata aaaatggggt tcactttttg gcaaagttac agtatgctta
    12661 ttacatataa atagagtgcc gataatggct ttttttcatc ttcgaaatac gcttgctact
    12721 gctcttccag cgtttttatt acttctttct tgtttctcct tagtatataa aatatcaagc
    12781 tacaacaagc atacaatcaa ctgtcaactg tcaattatat tataatacac tatgcatctt
    12841 tctacattgc tcctccccct cctccccacc gccgccctct ccgcaatctg ctaccccgag
    12901 acaggcggct cgaactgcgc ctcgctcctc agcatcaaag aattctactc ccttcaatac
    12961 tgcacctacc ggtggaacgt gctttacggc gactgggacc acttcgtcaa caacgcgacc
    13021 agcccgacga aggtgcacgc cagcgtgggt aagacgggcg tgtttgatag cttcgaggac
    13081 tgtcttaatg ggtttgagga tgtcgtggag acatgtcatg gtgttagtca gggtggggtt
    13141 atgaccaatg ggaattgagg agattgataa gacttttcta gttgcatatc ttttatattt
    13201 aaatcttatc tattagttaa ttttttgtaa tttatcctta tatatagtct ggttattcta
    13261 aaatatcatt tcagtatcta aaaattcccc tcttttttca gttatatctt aacaggcgac
    13321 agtccaaatg ttgatttatc ccagtccgat tcatcagggt tgtgaagcat tttgtcaatg
    13381 gtcgaaatca catcagtaat agtgcctctt acttgcctca tagaatttct ttctcttaac
    13441 gtcaccgttt ggtcttttat agtttcgaaa tctatggtga taccaaatgg tgttcccaat
    13501 tcatcgttac gggcgtattt tttaccaatt gaagtattgg aatcgtcaat tttaaagtat
    13561 atctctcttt tacgtaaagc ctgcgagatc ctcttaagta tagcggggaa gccatcgtta
    13621 ttcgatattg tcgtaacaaa tactttgatc ggcgctatgc ggccgccacc gcggtggagc
    13681 tccagctttt gttcccttta gtgagggtta attgcgcgct tggcgagact caaacaagat
    13741 atattttgcc cgccttacag acgaaactag ttggaggtta tggagcatac tatcacgtgg
    13801 gcggccactg gtgagttact acaccccagg ggcaacgttg atgctcctaa aaaactctgg
    13861 ctggacgcaa gccgtaacac ccgtgtcact tcataatcgt ttgcaattca gggcttgatc
    13921 tacacattac cctgttatcc ctattaatta atacggttat ccacagaatc aggggataac
    13981 gcaggaaaga acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg
    14041 ttgctggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca
    14101 agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc
    14161 tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc
    14221 ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag
    14281 gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc
    14341 ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca
    14401 gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg
    14461 aagtggtggc ctaactacgg ctacactaga agaacagtat ttggtatctg cgctctgctg
    14521 aagccagtta ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct
    14581 ggtagcggtg gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa
    14641 gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa
    14701 gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa
    14761 tgaagtttta aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc
    14821 ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga
    14881 ctccccgtcg tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca
    14941 atgataccgc gagacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc
    15001 ggaagggccg agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat
    15061 tgttgccggg aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc
    15121 attgctacag gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt
    15181 tcccaacgat caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc
    15241 ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg
    15301 gcagcactgc ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt
    15361 gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg
    15421 gcgtcaatac gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga
    15481 aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg
    15541 taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg
    15601 tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt
    15661 tgaatactca tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc
    15721 atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca
    15781 tttccccgaa aagtgccacc tgaacgaagc atctgtgctt cattttgtag aacaaaaatg
    15841 caacgcgaga gcgctaattt ttcaaacaaa gaatctgagc tgcattttta cagaacagaa
    15901 atgcaacgcg aaagcgctat tttaccaacg aagaatctgt gcttcatttt tgtaaaacaa
    15961 aaatgcaacg cgagagcgct aatttttcaa acaaagaatc tgagctgcat ttttacagaa
    16021 cagaaatgca acgcgagagc gctattttac caacaaagaa tctatacttc ttttttgttc
    16081 tacaaaaatg catcccgaga gcgctatttt tctaacaaag catcttagat tacttttttt
    16141 ctcctttgtg cgctctataa tgcagtctct tgataacttt ttgcactgta ggtccgttaa
    16201 ggttagaaga aggctacttt ggtgtctatt ttctcttcca taaaaaaagc ctgactccac
    16261 ttcccgcgtt tactgattac tagcgaagct gcgggtgcat tttttcaaga taaaggcatc
    16321 cccgattata ttctataccg atgtggattg cgcatacttt gtgaacagaa agtgatagcg
    16381 ttgatgattc ttcattggtc agaaaattat gaacggtttc ttctattttg tctctatata
    16441 ctacgtatag gaaatgttta cattttcgta ttgttttcga ttcactctat gaatagttct
    16501 tactacaatt tttttgtcta aagagtaata ctagagataa acataaaaaa tgtagaggtc
    16561 gagtttagat gcaagttcaa ggagcgaaag gtggatgggt aggttatata gggatatagc
    16621 acagagatat atagcaaaga gatacttttg agcaatgttt gtggaagcgg tattcgcaat
    16681 attttagtag ctcgttacag tccggtgcgt ttttggtttt ttgaaagtgc gtcttcagag
    16741 cgcttttggt tttcaaaagc gctctgaagt tcctatactt tctagagaat aggaacttcg
    16801 gaataggaac ttcaaagcgt ttccgaaaac gagcgcttcc gaaaatgcaa cgcgagctgc
    16861 gcacatacag ctcactgttc acgtcgcacc tatatctgcg tgttgcctgt atatatatat
    16921 acatgagaag aacggcatag tgcgtgttta tgcttaaatg cgtacttata tgcgtctatt
    16981 tatgtaggat gaaaggtagt ctagtacctc ctgtgatatt atcccattcc atgcggggta
    17041 tcgtatgctt ccttcagcac taccctttag ctgttctata tgctgccact cctcaattgg
    17101 attagtctca tccttcaatg ctatcatttc ctttgatatt ggatcatact aagaaaccat
    17161 tattatcatg acattaacct ataaaaatag gcgtatcacg aggccctttc gtctcgcgcg
    17221 tttcggtgat gacggtgagc gcgtttcggt gatgacggtg aaaacctcgg cgcgccatta
    17281 ccctgttatc cctaatatac aacgcaaaga agctggaaaa ttggcgaggg aatcctgttt
    17341 ctgtctatcc aagaatgggc atgaggtggc aaccgtcgtg ctagcgtaca gggtgcactt
    17401 tgtaaccatt tgggacaccg gacactcgct gttttcgaaa ttacccttta agcgcgggta
    17461 ttgaaccagg cttatgccca agatcgtagc aagcatttaa atgacggtat cgataagctt
    17521 gatatcgaat tcctgcagcc cgggg
//
