LOCUS       sequence-212576-       10640 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of a fungal biosynthetic gene cluster.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10640)
  AUTHORS   Harvey CJB, Tang M, Schlecht U, Horecka J, Fischer CR, Lin HC, Li J,
            Naughton B, Cherry J, Miranda M, Li YF, Chu AM, Hennessy JR, Vandova
            GA, Inglis D, Aiyar RS, Steinmetz LM, Davis RW, Medema MH, Sattely 
            E, Khosla C, St Onge RP, Tang Y, Hillenmeyer ME
  TITLE     HEx: A heterologous expression platform for the discovery of fungal 
            natural products.
  JOURNAL   Sci Adv. 2018 Apr 11;4(4):eaar5459. doi: 10.1126/sciadv.aar5459. 
            eCollection 2018 Apr.
  PUBMED    29651464
REFERENCE   2  (bases 1 to 10640)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Adv."; 
            date: "2018-04-11"; volume: "4(4):eaar5459. doi"; pages: " 
            10.1126/sciadv.aar5459. eCollection 2018 Apr"
COMMENT     Sequence Label: pCHSU65.2-2.2
FEATURES             Location/Qualifiers
     source          1..10640
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      1297..1596
                     /label=ADH2 terminator
                     /note="transcription terminator for the S. cerevisiae 
                     alcohol dehydrogenase 2 (ADH2) gene"
     promoter        3434..3654
                     /gene="S. cerevisiae URA3"
                     /label=URA3 promoter
     CDS             3655..4458
                     /codon_start=1
                     /gene="S. cerevisiae URA3"
                     /product="orotidine-5'-phosphate decarboxylase, required 
                     for uracil biosynthesis"
                     /label=URA3
                     /note="yeast auxotrophic marker, counterselectable with 
                     5-fluoroorotic acid (5-FOA)"
                     /translation="MSKATYKERAATHPSPVAAKLFNIMHEKQTNLCASLDVRTTKELL
                     ELVEALGPKICLLKTHVDILTDFSMEGTVKPLKALSAKYNFLLFEDRKFADIGNTVKLQ
                     YSAGVYRIAEWADITNAHGVVGPGIVSGLKQAAEEVTKEPRGLLMLAELSCKGSLSTGE
                     YTKGTVDIAKSDKDFVIGFIAQRDMGGRDEGYDWLIMTPGVGLDDKGDALGQQYRTVDD
                     VVSTGSDIIIVGRGLFAKGRDAKVEGERYRKAGWEAYLRRCGQQN"
     primer_bind     complement(6550..6570)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(6550..6568)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     rep_origin      complement(6907..7495)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6987..7006)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7666..8457,8458..8526))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8289..8308
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8527..8631)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      8658..10000
                     /label=2u ori
                     /note="yeast 2u plasmid origin of replication"
     protein_bind    complement(9621..9668)
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     primer_bind     10041..10059
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(10401..10417)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(10401..10417)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
ORIGIN
        1 cttataagtt ggagaaataa gagaatttca gattgagaga atgaaaaaaa aaaaaaaaaa
       61 aaaggcagag gagagcatag aaatggggtt cactttttgg taaagctata gcatgcctat
      121 cacatataaa tagagtgcca gtagcgactt ttttcacact cgaaatactc ttactactgc
      181 tctcttgttg tttttatcac ttcttgtttc ttcttggtaa atagaatatc aagctacaaa
      241 aagcatacaa tcaactatca actattaact atatcgtaat acacaatgtc gatgacatca
      301 ggcaacacca aggataaggg cggctacaga caaatcaaca agtccctcaa catctgcgcc
      361 tttgaagatt acctcaacgg ccaaacagca gggttgaagc ctatcccaga tgtcgaacaa
      421 gtcacaccaa gagtgatgcg tgtacgagga cagaaccccg gcaagttcac aatgcaaggc
      481 acaaacacgt tcctagtcgg cactggcgct tctcgcatcc tcatcgacac ctcaggcggc
      541 gagccagaat acgcaaagct actcgcatcc actctggaat tcctaaacat cagcatcaaa
      601 tacgtcctcc tcacacactg gcacggcgac cacacgggcg gagtcccaga cctactccgc
      661 ctctaccccc acctagaaaa ccacatctac aagaacatac cagaggtcgg ccaccgcgac
      721 atctcagacg gtcaactgtt ccacgtcgag ggcgcaacac tcgtggctgt acacacaccg
      781 ggccacgccg aagatcacat gtgctttgtg ctcaaggaag aaaactcaat gttcactggt
      841 gacaacatcc tcggtacggg taccagcgca gtcgaggact tgggtatctt catgtccagt
      901 ctgcaaaaga tgttgggaaa gaactgccaa accggacacc ccgcccacgg ccaaaccatc
      961 ggtgacctaa acgcaaagat atcaaaggaa ctcatgtcaa agttccgacg agagaagcag
     1021 gtactgcagg ccatgcgcga cctacgggat gctgatgaga ggcggccaaa cgttcgaatg
     1081 attgtcgatc gcatgtatgg aagctccgtc aacgagacta cgcggacatt ggccttggag
     1141 ccgtttattg gcgaggtact gcggaaactt gctggtgatg gcaaggttgg gtttggcgtg
     1201 cgggctggga ggaagggctg gttcctggtt ccaaatgagc agcagagtac gcagttacgg
     1261 catacacatt cggtttttgt aagagctagt gcataggcgg atctcttatg tctttacgat
     1321 ttatagtttt cattatcaag tatgcctata ttagtatata gcatctttag atgacagtgt
     1381 tcgaagtttc acgaataaaa gataatattc tactttttgc tcccaccgcg tttgctagca
     1441 cgagtgaaca ccatccctcg cctgtgagtt gtacccattc ctctaaactg tagacatggt
     1501 agcttcagca gtgttcgtta tgtacggcat cctccaacaa acagtcggtt atagtttgtc
     1561 ctgctcctct gaatcgtctc cctcgatatt tctcattttc cttcgcatgc cagcattgaa
     1621 atgatcgaag ttcaatgatg aaacggtaat tcttctgtca tttactcatc tcatctcatc
     1681 aagttatata attctatacg gatgtaattt ttcacttttc gtcttgacgt ccaccctata
     1741 atttcaatta ttgaaccctc acgatccagt tctccagtga cacagccttt atctggtcaa
     1801 acctttcttt ctaatcacct atgctgatgc ttaattaagg gatttttgtc tccatcaacg
     1861 gcatgcgccc aaaaatgacg ttttttttaa cccatagaca cgaaactacc cattttccac
     1921 cggcctgacc taccaccgga acaacggcca tctccaactt gcaagttggg gaaattaaga
     1981 gcatcgcagg tttaatggaa gaaaaaaaaa aggtacagca cagcgcaaat ggagttagtt
     2041 cccttatgtc acacactcac acacagtcgg tcagatcaag catactgggt gcgtataaat
     2101 agagtggcca ttgccaccct gtttatctca aaatctgtct tgttagtggt cttctccctt
     2161 tttcaggtta caattctctt gtttctactt agtatataag tatatcaagc tatattaagc
     2221 atactatcaa ctgtcaactc tatcctcaaa atacaataca aaatggataa cacgaccctc
     2281 ttcgacgtca agaacaaaat tgtcctcgtg accggcggcg ccaaaggcat cggccgcatg
     2341 ataagcgaag gtttcatcca aaacggcgca aacgtctaca tctcgtcccg cgacgcgaaa
     2401 gcgtgcgagc aagcatgtaa agagctgaat gcactgggaa aaggcaaagc agagtttatc
     2461 gttgcggatt tgtatcggga ggatgcggcg agttttgttg cgggggagtt gcagaagaga
     2521 gtgggaaaac tcgacgtcct agtgaacaac agtgggagta actggggcga gtcatacgac
     2581 acatacccat cgtccgcctg ggaccgcgtt ctaaccctaa acctaaaacg cgttttccaa
     2641 ctcactcaag ccgttacacc ccttctctct gctgccgcct ccccctcgtc ccccgcacgc
     2701 atcatcaaca ttggcagcat agacggtctc cgtgtcccag ctctcgaaac gtttgcctat
     2761 tcagcctcta aagcgggtct gcatcacatg agcaaagtac tagcgaacca tttgggaaag
     2821 aggaatatca cggtgaatac cgtggcgtgt gggccgtttg agagtaagat gatgaaggcg
     2881 acgctggaga agcatagggg cgttattgag gaggggattc cgctggggag gattggaggg
     2941 aaggaggatg tagcgggtac gtgtatttgg ttggcgagtc gcgctggggc gtgggttaat
     3001 gggattgtgg tggcattgga tggggggagt gttattggag ctaagctttg aacaaatcgc
     3061 tcttaaatat atacctaaag aacattaaag ctatattata agcaaagata cgtaaatttt
     3121 gcttatatta ttatacacat atcatatttc tatattttta agatttggtt atataatgta
     3181 cgtaatgcaa aggaaataaa ttttatacat tattgaacag cgtccaagta actacattat
     3241 gtgcactaat agtttagcgt cgtgaagact ttattgtgtc gcgaaaagta aaaattttaa
     3301 aaattagagc accttgaact tgcgaaaaag gttctcatca actgtttaaa aggaggatat
     3361 caggtcctat ttctgacaaa caatatacaa atttagtttc aaagatgaat cagtgcgcga
     3421 aggacataac tcattcaatt caattcatca tttttttttt attctttttt ttgatttcgg
     3481 tttccttgaa atttttttga ttcggtaatc tccgaacaga aggaagaacg aaggaaggag
     3541 cacagactta gattggtata tatacgcata tgtagtgttg aagaaacatg aaattgccca
     3601 gtattcttaa cccaactgca cagaacaaaa acctgcagga aacgaagata aatcatgtcg
     3661 aaagctacat ataaggaacg tgctgctact catcctagtc ctgttgctgc caagctattt
     3721 aatatcatgc acgaaaagca aacaaacttg tgtgcttcat tggatgttcg taccaccaag
     3781 gaattactgg agttagttga agcattaggt cccaaaattt gtttactaaa aacacatgtg
     3841 gatatcttga ctgatttttc catggagggc acagttaagc cgctaaaggc attatccgcc
     3901 aagtacaatt ttttactctt cgaagacaga aaatttgctg acattggtaa tacagtcaaa
     3961 ttgcagtact ctgcgggtgt atacagaata gcagaatggg cagacattac gaatgcacac
     4021 ggtgtggtgg gcccaggtat tgttagcggt ttgaagcagg cggcagaaga agtaacaaag
     4081 gaacctagag gccttttgat gttagcagaa ttgtcatgca agggctccct atctactgga
     4141 gaatatacta agggtactgt tgacattgcg aagagcgaca aagattttgt tatcggcttt
     4201 attgctcaaa gagacatggg tggaagagat gaaggttacg attggttgat tatgacaccc
     4261 ggtgtgggtt tagatgacaa gggagacgca ttgggtcaac agtatagaac cgtggatgat
     4321 gtggtctcta caggatctga cattattatt gttggaagag gactatttgc aaagggaagg
     4381 gatgctaagg tagagggtga acgttacaga aaagcaggct gggaagcata tttgagaaga
     4441 tgcggccagc aaaactaaaa aactgtatta taagtaaatg catgtatact aaactcacaa
     4501 attagagctt caatttaatt atatcagtta ttaccctatg cggtgtgaaa taccgcacag
     4561 atgcgtaagg agaaaatacc gcatcaggat aggaaaaaac cgagcttcct ttcatccggc
     4621 gcggctgtgt tctacatatc actgaagctc cgggtatttt aagttataca agggaaagat
     4681 gccggctaga ctagcaagtt ttaggctgct taacattatg gataggcgga taaagggccc
     4741 aaacaggatt gtaaagctta gacgcttctg gttggacaat ggtacgtttg tgtattaagt
     4801 aaggcttggc tggggatagc aacattgggc agagtataga agaccacaaa aaaaaggtat
     4861 ataagggcag agaagtcttt gtaatgtgtg taacttctct tccatgtgta atcagtattt
     4921 ctacttactt cttaaatata cagaagtaag acagataacc aacagccttt cccagatata
     4981 catatatatc tttatttcag cttaaacaat aattatattt gtttaactca aaaataaaaa
     5041 aaaaaaaacc aaactcacgc aactaattat tccataataa aataacaaca tggagagccc
     5101 ttttgtgatg ccgcaggagg atgtggaggc gctgggtcag tcaaaggcgt tttgcgcgct
     5161 gaggaggtgt cttgctggtg aggccgcgtt ttgcgcaaca cacgcgcatt gcacacccca
     5221 agccaaacaa acatggctcc ttcaccgcga cattctccac gccctcatca tgcctgtagt
     5281 gacgctcttt tcgcgcgcct caactctggc gtcggccgcc ctctgtacgc agcgaacgtc
     5341 ggacctcgaa cttgcgtttt cgggtgaatc gcgcagcgct ttcataggcc tgcaatgctt
     5401 catcagtgag gaggctgact ggtgccgcac gagagggtgt ccggcttgcg tcgtctcggc
     5461 cacgttgagc accgacagcc atatccgcct gacaattgct gcgagcctgc tcagcactgc
     5521 gagtgtagcc acgcccacgc aagaggaacc tcgacacgac agtgatagga cgctgcctcc
     5581 actcccgcat atccttcctg ccctgcaaca cgccgtgata aacgacccgt tctgggaagg
     5641 ctcggacatc tggggctaca tcttgtcgcg tgccacccaa ctgtcggctg gcatacaagc
     5701 cctaatcgcc gagtgtgtga atcttgaatc gctcgtctca tcgcccacaa cagacaggcc
     5761 gggtgcgaag cgcggcttga cgcatccctc ggtgcctttt ggagcttctg gccaggccgt
     5821 cgaggaaaag ggcgtcaaac tcagaaagag caagctagca aagcggcagt tgaggctacg
     5881 agacgaggag gttgagctga tgaggagagt agccatgcaa tgctgggcca aagcccaggt
     5941 accaaagaaa atcagaggcg atgtcttggg cataagcgat ggcaagagga cgaggagctt
     6001 gacttgccca tgaggagatt gataagactt ttctagttgc atatctttta tatttaaatc
     6061 ttatctatta gttaattttt tgtaatttat ccttatatat agtctggtta ttctaaaata
     6121 tcatttcagt atctaaaaat tcccctcttt tttcagttat atcttaacag gcgacagtcc
     6181 aaatgttgat ttatcccagt ccgattcatc agggttgtga agcattttgt caatggtcga
     6241 aatcacatca gtaatagtgc ctcttacttg cctcatagaa tttctttctc ttaacgtcac
     6301 cgtttggtct tttatagttt cgaaatctat ggtgatacca aatggtgttc ccaattcatc
     6361 gttacgggcg tattttttac caattgaagt attggaatcg tcaattttaa agtatatctc
     6421 tcttttacgt aaagcctgcg agatcctctt aagtatagcg gggaagccat cgttattcga
     6481 tattgtcgta acaaatactt tgatcggcgc tatgcggccg ccaccgcggt ggagctccag
     6541 cttttgttcc ctttagtgag ggttaattgc gcgcttggcg agactcaaac aagatatatt
     6601 ttgcccgcct tacagacgaa actagttgga ggttatggag catactatca cgtgggcggc
     6661 cactggtgag ttactacacc ccaggggcaa cgttgatgct cctaaaaaac tctggctgga
     6721 cgcaagccgt aacacccgtg tcacttcata atcgtttgca attcagggct tgatctacac
     6781 attaccctgt tatccctatt aattaatacg gttatccaca gaatcagggg ataacgcagg
     6841 aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct
     6901 ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca
     6961 gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct
     7021 cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc
     7081 gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt
     7141 tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc
     7201 cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc
     7261 cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg
     7321 gtggcctaac tacggctaca ctagaagaac agtatttggt atctgcgctc tgctgaagcc
     7381 agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag
     7441 cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga
     7501 tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat
     7561 tttggtcatg agattatcaa aaaggatctt cacctagatc cttttaaatt aaaaatgaag
     7621 ttttaaatca atctaaagta tatatgagta aacttggtct gacagttacc aatgcttaat
     7681 cagtgaggca cctatctcag cgatctgtct atttcgttca tccatagttg cctgactccc
     7741 cgtcgtgtag ataactacga tacgggaggg cttaccatct ggccccagtg ctgcaatgat
     7801 accgcgagac ccacgctcac cggctccaga tttatcagca ataaaccagc cagccggaag
     7861 ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc atccagtcta ttaattgttg
     7921 ccgggaagct agagtaagta gttcgccagt taatagtttg cgcaacgttg ttgccattgc
     7981 tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct tcattcagct ccggttccca
     8041 acgatcaagg cgagttacat gatcccccat gttgtgcaaa aaagcggtta gctccttcgg
     8101 tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg ttatggcagc
     8161 actgcataat tctcttactg tcatgccatc cgtaagatgc ttttctgtga ctggtgagta
     8221 ctcaaccaag tcattctgag aatagtgtat gcggcgaccg agttgctctt gcccggcgtc
     8281 aatacgggat aataccgcgc cacatagcag aactttaaaa gtgctcatca ttggaaaacg
     8341 ttcttcgggg cgaaaactct caaggatctt accgctgttg agatccagtt cgatgtaacc
     8401 cactcgtgca cccaactgat cttcagcatc ttttactttc accagcgttt ctgggtgagc
     8461 aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat
     8521 actcatactc ttcctttttc aatattattg aagcatttat cagggttatt gtctcatgag
     8581 cggatacata tttgaatgta tttagaaaaa taaacaaata ggggttccgc gcacatttcc
     8641 ccgaaaagtg ccacctgaac gaagcatctg tgcttcattt tgtagaacaa aaatgcaacg
     8701 cgagagcgct aatttttcaa acaaagaatc tgagctgcat ttttacagaa cagaaatgca
     8761 acgcgaaagc gctattttac caacgaagaa tctgtgcttc atttttgtaa aacaaaaatg
     8821 caacgcgaga gcgctaattt ttcaaacaaa gaatctgagc tgcattttta cagaacagaa
     8881 atgcaacgcg agagcgctat tttaccaaca aagaatctat acttcttttt tgttctacaa
     8941 aaatgcatcc cgagagcgct atttttctaa caaagcatct tagattactt tttttctcct
     9001 ttgtgcgctc tataatgcag tctcttgata actttttgca ctgtaggtcc gttaaggtta
     9061 gaagaaggct actttggtgt ctattttctc ttccataaaa aaagcctgac tccacttccc
     9121 gcgtttactg attactagcg aagctgcggg tgcatttttt caagataaag gcatccccga
     9181 ttatattcta taccgatgtg gattgcgcat actttgtgaa cagaaagtga tagcgttgat
     9241 gattcttcat tggtcagaaa attatgaacg gtttcttcta ttttgtctct atatactacg
     9301 tataggaaat gtttacattt tcgtattgtt ttcgattcac tctatgaata gttcttacta
     9361 caattttttt gtctaaagag taatactaga gataaacata aaaaatgtag aggtcgagtt
     9421 tagatgcaag ttcaaggagc gaaaggtgga tgggtaggtt atatagggat atagcacaga
     9481 gatatatagc aaagagatac ttttgagcaa tgtttgtgga agcggtattc gcaatatttt
     9541 agtagctcgt tacagtccgg tgcgtttttg gttttttgaa agtgcgtctt cagagcgctt
     9601 ttggttttca aaagcgctct gaagttccta tactttctag agaataggaa cttcggaata
     9661 ggaacttcaa agcgtttccg aaaacgagcg cttccgaaaa tgcaacgcga gctgcgcaca
     9721 tacagctcac tgttcacgtc gcacctatat ctgcgtgttg cctgtatata tatatacatg
     9781 agaagaacgg catagtgcgt gtttatgctt aaatgcgtac ttatatgcgt ctatttatgt
     9841 aggatgaaag gtagtctagt acctcctgtg atattatccc attccatgcg gggtatcgta
     9901 tgcttccttc agcactaccc tttagctgtt ctatatgctg ccactcctca attggattag
     9961 tctcatcctt caatgctatc atttcctttg atattggatc atactaagaa accattatta
    10021 tcatgacatt aacctataaa aataggcgta tcacgaggcc ctttcgtctc gcgcgtttcg
    10081 gtgatgacgg tgagcgcgtt tcggtgatga cggtgaaaac ctcggcgcgc cattaccctg
    10141 ttatccctaa tatacaacgc aaagaagctg gaaaattggc gagggaatcc tgtttctgtc
    10201 tatccaagaa tgggcatgag gtggcaaccg tcgtgctagc gtacagggtg cactttgtaa
    10261 ccatttggga caccggacac tcgctgtttt cgaaattacc ctttaagcgc gggtattgaa
    10321 ccaggcttat gcccaagatc gtagcaagca tttaaatgac ggtatcgata agcttgatat
    10381 cgaattcctg cagcccgggg gatccactag ttctagatta attaatatct aaaaattgcc
    10441 ttatgatccg tctctccggt tacagcctgt gtaactgatt aatcctgcct ttctaatcac
    10501 cattctaatg ttttaattaa gggattttgt cttcattaac ggctttcgct cataaaaatg
    10561 ttatgacgtt ttgcccgcag gcgggaaacc atccacttca cgagactgat ctcctctgcc
    10621 ggaacaccgg gcatctccaa
//
