LOCUS       sequence-21258-e         661 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 661)
  AUTHORS   Fiordalisi JJ, Johnson RL 2nd, Ulku AS, Der CJ, Cox AD
  TITLE     Mammalian expression vectors for Ras family proteins: generation and
            use of expression constructs to analyze Ras family function.
  JOURNAL   Methods Enzymol. 2001;332:3-36. doi: 10.1016/s0076-6879(01)32189-4.
  PUBMED    11305105
REFERENCE   2  (bases 1 to 661)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Methods 
            Enzymol."; date: "2001"; volume: "332:3-36. doi"; pages: " 
            10.1016/s0076-6879(01)32189-4"
COMMENT     Sequence Label: pCGN N-Ras 12D, 189L
FEATURES             Location/Qualifiers
     source          1..661
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     1..25
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     5'UTR           94..133
                     /label=HSV TK 5'-UTR
                     /note="5' untranslated region from the herpes simplex virus
                     thymidine kinase gene"
     CDS             163..189
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             169..195
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /note="This is a variant of the standard HA tag, which has 
                     sequence YPYDVPDYA. Some anti-HA antibodies may not 
                     recognize this variant."
                     /translation="YDVPDYASL"
ORIGIN
        1 agctcgttta gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca
       61 tagaagacac cgggaccgat ccagcctccg ggggatcttg gtggcgtgaa actcccgcac
      121 ctcttcggcc agcgccttgt agaagcgcgt atggcttcta gctatcctta tgacgtgcct
      181 gactatgcca gcctgggagg accttctagc ggatccacca tgactgagta caaactggtg
      241 gtggttggag cagatggtgt tgggaaaagc gcactgacaa tccagctaat ccagaaccac
      301 tttgtagatg aatatgatcc caccatagag gattcttaca gaaaacaagt ggttatagat
      361 ggtgaaacct gtttgttgga catactggat acagctggac aagaagagta cagtgccatg
      421 agagaccaat acatgaggac aggcgaaggc ttcctctgtg tatttgccat caataatagc
      481 aagtcatttg cggatattaa cctctacagg gagcagatta agcgagtaaa agactcggat
      541 gatgtaccta tggtgctagt gggaaacaag tgtgatttgc caacaaggac agttgataca
      601 aaacaagccc acgaactggc caagagttac gggattccat tcattgaaac ctcagccaag
      661 a
//
