LOCUS       sequence-212580-        8567 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses the active zone - enriched protein Sentryn-GFP in a subset
            of cholinergic ventral nerve cord motor neurons.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8567)
  AUTHORS   Edwards SL, Morrison LM, Manning L, Stec N, Richmond JE, Miller KG
  TITLE     Sentryn Acts with a Subset of Active Zone Proteins To Optimize the 
            Localization of Synaptic Vesicles in Caenorhabditis elegans.
  JOURNAL   Genetics. 2018 Nov;210(3):947-968. doi: 10.1534/genetics.118.301466.
  PUBMED    30401765
REFERENCE   2  (bases 1 to 8567)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1534/genetics.118"; journalName: "Genetics"; date: "2018-11"; 
            volume: "210"; issue: "3"; pages: "947-968"
COMMENT     Sequence Label: KG#797
FEATURES             Location/Qualifiers
     source          1..8567
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(4188..4216)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
     primer_bind     4964..4984
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
     primer_bind     complement(6006..6028)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     6128..6147
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(6225..6243)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        6311..6415
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6416..6484,6485..7276)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6634..6653)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7447..8035
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7936..7955
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8189..8206
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    8323..8344
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(8359..8364,8365..8382,8383..8389)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8397..8413
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     8402..8424
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     8421..8437
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     8421..8437
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 agcttgggct gcagcccggg aaacatgata tcgacggaca tatttttgat tgattatcat
       61 aattttcgtg ggaatcttcg gaacatgctc tttgcggttt ataagtggag atgcgccaac
      121 gggaaacttg ttttaaaact attcctatgg tgctaacatc accacatgaa aacttttcaa
      181 aacttttttg ggttttttat ttttttatta ttattcgtat ctaaacattg taggcgttgt
      241 gatgttgccc taaatttctt taaaaagagc gaacattttc aaaaatttgc tatttaaatc
      301 gaatgaaaat ttccattcaa atatttgaaa aaaattatga aaaaattcct tttgttgcat
      361 cttcatcttc tatgaaagtt acaattttag ttggatcaga aatatgagat atcaaaaaaa
      421 aattaagatt tttttgtttg tttttttgaa gcctaaaata ttttgatatc atttatctct
      481 tatgtctttt gttttatcga aacgtttaca acacatcaac tatgcgtcaa taaatttttt
      541 aggtagttaa acaaatgttg aaaaaaaaag caaaagcaac caagagctgt taaaatagag
      601 aaaatgcagt tattttttat attcaagaaa agttatcaca gaagttccgt aatgagtttt
      661 ttccttatca acatgttccc atcaacttct tagtttctcc tcattcccat tgttcaacat
      721 aattatcacc tctgacctta ttctgctctt tttaagcaca ggtgttaata tgccgtccat
      781 catatttgcc ctccgtctgc cgctttcctt atctgacctt tacatcgcct ttttgtatat
      841 gtacataata attgtatcat catcaacctc cgccataaac atgcatgtct ttttacctct
      901 tttggcatgt accgttcttc tttgtatttt tattatgtct tccttccatc ttccctcttt
      961 tttacggatt ttcggattgc ggaagaagaa gatggtcgat aattaccgta atgaggtaat
     1021 ttgaaacata ttggacagaa gaaatacgcg cggatgcttt cggtggcaag gttaattcgt
     1081 tgtctttgtt tctggaatgt ccttcataaa aaacacggat taccacctgt ttttaacgtg
     1141 gattaagatt tttggaaaca atttactctg cctaattttt ttgtacattt aaaaatgtag
     1201 acttaccgaa ttcaccgagc ttgaccagtt ttaaaggtta ccgttcacac taaaaattgt
     1261 tgaaaaaatt ttcgactaaa cctaatgatt ttattttaaa aaataaatga aatagcatac
     1321 aaatttggat ttttttcaat ttttttcaag aaaatcatga aacatttaga aaactcgagt
     1381 aacaagcttt ccgttcattc tgtaattttt aagttccaaa tttaaaaaaa tgcattttga
     1441 tttactggga ttgccgtgct cgtaattatc agcaaaacat ttgccgaatt agccgagctc
     1501 gacaatctat tttttcgcaa acttttgcca tgtttcgtaa aacaattttc cattttatgg
     1561 tagacatttt tcatcaatga attcacaaaa tttaaaatcc ccactgaaag taccaaaatc
     1621 ttcctgaaaa atccggcaaa aaatccatct cattatcatg tcacatatac atgttctatt
     1681 ggcactatgt ttgggatgtc ctccaattaa tacttctgtg tcaatctcat cggcgcccgg
     1741 cggccactac caaatgcaaa actttgtttg taaaagaaga agccaataat tttctcgtta
     1801 ttcattttta ttactctttt tgcgtttcat tcttctttgt tcgaattgat gaacgcacgt
     1861 tgcttctctc tggttttcct gccgttcctt atattgccaa ggaaaccaat gcggattttc
     1921 ggaagatttt cttcatgtcc tttagaaaat atcaccactt ttcttctttt tccatttcaa
     1981 atttcaattt ccatacaact catcttctat tatagctttt caggtgttcg actgtttgaa
     2041 gataagaatc aattaaaatt tggttcactg agcatgtcat ccacttttcc ttttctttgt
     2101 tctaaaattc accaaactga attttcctta accggaaatt tacatgaaaa taagaaacat
     2161 ggattacatc ctttacatat catttttgtt tttttatgtg tattccatgt tcccaaaagt
     2221 ttgatgcaat ttttgattct ttttcaattt tccaaattcg tgtctcgcag ggaacgattc
     2281 acagtccaac attcccaaat tgcgaaattt tagaatatat attcccaagc gtcgaatatc
     2341 tcaaaaattt gtcgttttct cagtttgaca tgtcaccttt gccggttcct ctattcgtct
     2401 attagctctc cagagagcaa tgtcatgtca cacgaagaat tcagggacat gtttcgtttt
     2461 tttttgtttg catgtggtca ccgatatggg aacttgaact ttccaatttt tgcttgcttt
     2521 ttcttttccg gaaacttaat actatttgag aaactccgca tcttatttgt ttgatcccaa
     2581 attctgaaat tcctctttga atttgatctc aagcttcact ataaaaaatt gtttcaggaa
     2641 aattggaaga gcaagcggat ccccgggatt ggccaaagga cccaaaggta tgtttcgaat
     2701 gatactaaca taacatagaa cattttcagg aggacccttg gctagcaaaa atgatgaact
     2761 ccacctcagg atcatccaca atacgagatt tggtgacacc aaagaattgc aactgggatt
     2821 ttgaatcatt taaccaacac catcaagaaa agcacaccag caagcagccc gcgtgtaatt
     2881 gtgttgctga agcaattcga cgcgtcactg ctcttcggaa gttggcaaca cagcaagatg
     2941 gagattttac aatggttcaa aacaatgaag tcaagtgggc aaaacttttt atggaattca
     3001 tcattggaac tgcgtattgt cagtctacaa agtgtcaagc ccactccgac gatatgattt
     3061 ggtatgttca ccaggcgaaa ctctccaatt ccttttacat tcaagggaat caaagcttgt
     3121 tgcaagtttt tcgtcgacac agcaaaaacc aaccaacgcc ttgcgatgac agctacaact
     3181 gggaggagac tgtttgcctg aacataatca tgcaacagat tgacttttat gttacctgtg
     3241 ccgtttgcac aaaaactgga cctcaaaatt tgcaaattat tcgcaaaaac tgtcagcgtg
     3301 tattcccatc cccgagtcgt cgtcgtatgg atagtaaagg agagaatgaa gagattacat
     3361 atcctaaatt atattttgca attgacggtt ttgaagaggt ttttaacgac gtaattgtgc
     3421 gtgacggaga gtgtgtatgt gttgaactgg ttgcacgtga tcgccagaaa actcgagagt
     3481 ccgttgtctt tctcggatca atacgatatg aaattctgaa acaagtgtat gacactaaag
     3541 cttcttcgac gtggcagtgg gcacaaaagt tgatgaaagt aggcgatcga cgtcaagaat
     3601 ttgtacgaat gcgcggtccg cagggcaaag gatatgcgga aatggcggtg gcaagagtgc
     3661 ccaactgcgg atacgaaacg ccgctcaccg aaaatcctat tgacttttct gagttggcag
     3721 gagtagaaca gtcacttgga aaacgtcgaa tgtcagagac aaatcttgcc ggcggaacac
     3781 ggatgttccc ttctcgttca attgccactc catctcattc atcgcgggga agacgttggc
     3841 aaagtgaggc ggacactgtg aaccaatacc ccgaagttga gggaagcaat atcgacgacg
     3901 aacttgacga aggcccaacg aacaagctct ggtcgatgcg aggttttgcg caagtttggc
     3961 atggacttcg agagaagaaa cgcgcagagt gcacaccact gaatgcattt ttgacctacg
     4021 tcacactgcc ctggaacagt attttaaatg atcttctgag tgaacgacaa aaacgtccga
     4081 ttttgacgtt cgagtttgat gcaccagctc ataatcacga ttcttccgga tcctctggag
     4141 cggtaccggt agaaaaaatg agtaaaggag aagaactttt cactggagtt gtcccaattc
     4201 ttgttgaatt agatggtgat gttaatgggc acaaattttc tgtcagtgga gagggtgaag
     4261 gtgatgcaac atacggaaaa cttaccctta aatttatttg cactactgga aaactacctg
     4321 ttccatgggt aagtttaaac atatatatac taactaaccc tgattattta aattttcagc
     4381 caacacttgt cactactttc tgttatggtg ttcaatgctt ctcgagatac ccagatcata
     4441 tgaaacggca tgactttttc aagagtgcca tgcccgaagg ttatgtacag gaaagaacta
     4501 tatttttcaa agatgacggg aactacaaga cacgtaagtt taaacagttc ggtactaact
     4561 aaccatacat atttaaattt tcaggtgctg aagtcaagtt tgaaggtgat acccttgtta
     4621 atagaatcga gttaaaaggt attgatttta aagaagatgg aaacattctt ggacacaaat
     4681 tggaatacaa ctataactca cacaatgtat acatcatggc agacaaacaa aagaatggaa
     4741 tcaaagttgt aagtttaaac atgattttac taactaacta atctgattta aattttcaga
     4801 acttcaaaat tagacacaac attgaagatg gaagcgttca actagcagac cattatcaac
     4861 aaaatactcc aattggcgat ggccctgtcc ttttaccaga caaccattac ctgtccacac
     4921 aatctgccct ttcgaaagat cccaacgaaa agagagacca catggtcctt cttgagtttg
     4981 taacagctgc tgggattaca catggcatgg atgaactata caaatagcat tcgtagaatt
     5041 gtaagtttaa actttctcat actaactaac gcgctctatt taaattttca gaattccaac
     5101 tgagcgccgg tcgctaccat taccaacttg tctggtgtca aaaataatag gggccgctgt
     5161 catcagagta agtttaaact gagttctact aactaacgag taatatttaa attttcagca
     5221 tctcgcgccc gtgcctctga cttctaagtc caattactct tcaacatccc tacatgctct
     5281 ttctccctgt gctcccaccc cctatttttg ttattatcaa aaaacttctc ttaatttctt
     5341 tgttttttag cttcttttaa gtcacctcta acaatgaaat tgtgtagatt caaaaataga
     5401 attaattcgt aataaaaagt cgaaaaaaat tgtgctccct ccccccatta ataataattc
     5461 tatcccaaaa tctacacaat gttctgtgta cacttcttat gttttttact tctgataaat
     5521 ttttttgaaa catcatagaa aaaaccgcac acaaaatacc ttatcatatg ttacgtttca
     5581 gtttatgacc gcaattttta tttcttcgca cgtctgggcc tctcatgacg tcaaatcatg
     5641 ctcatcgtga aaaagttttg gagtattttt ggaatttttc aatcaagtga aagtttatga
     5701 aattaatttt cctgcttttg ctttttgggg tttcccctat tgtttgtcaa gatttcgagg
     5761 acggcgtttt tcttgctaaa atcacaagta ttgatgagca cgatgcaaga aagatcggaa
     5821 gaaggtttgg gtttgaggct cagtggaagg tgagtagaag ttgataattt gaaagtggag
     5881 tagtgtctat ggggtttttg ccttaaatga cagaatacat tcccaatata ccaaacataa
     5941 ctgtttccta ctagtcggcc gtacgggccc tttcgtctcg cgcgtttcgg tgatgacggt
     6001 gaaaacctct gacacatgca gctcccggag acggtcacag cttgtctgta agcggatgcc
     6061 gggagcagac aagcccgtca gggcgcgtca gcgggtgttg gcgggtgtcg gggctggctt
     6121 aactatgcgg catcagagca gattgtactg agagtgcacc atatgcggtg tgaaataccg
     6181 cacagatgcg taaggagaaa ataccgcatc aggcggcctt aagggcctcg tgatacgcct
     6241 atttttatag gttaatgtca tgataataat ggtttcttag acgtcaggtg gcacttttcg
     6301 gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc
     6361 gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga agagtatgag
     6421 tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc ttcctgtttt
     6481 tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt
     6541 gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc gccccgaaga
     6601 acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat tatcccgtat
     6661 tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg acttggttga
     6721 gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag aattatgcag
     6781 tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa cgatcggagg
     6841 accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc gccttgatcg
     6901 ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca cgatgcctgt
     6961 agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc tagcttcccg
     7021 gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc tgcgctcggc
     7081 ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg
     7141 tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta tctacacgac
     7201 ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag gtgcctcact
     7261 gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga ttgatttaaa
     7321 acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa
     7381 aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg
     7441 atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc
     7501 gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac
     7561 tggcttcagc agagcgcaga taccaaatac tgttcttcta gtgtagccgt agttaggcca
     7621 ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt
     7681 ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc
     7741 ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg
     7801 aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc
     7861 cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac
     7921 gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct
     7981 ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc
     8041 cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt
     8101 tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac
     8161 cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg
     8221 cccaatacgc aaaccgcctc tccccgcgcg ttggccgatt cattaatgca gctggcacga
     8281 caggtttccc gactggaaag cgggcagtga gcgcaacgca attaatgtga gttagctcac
     8341 tcattaggca ccccaggctt tacactttat gcttccggct cgtatgttgt gtggaattgt
     8401 gagcggataa caatttcaca caggaaacag ctatgaccat gattacgcca agctgtaagt
     8461 ttaaacatga tcttactaac taactattct catttaaatt ttcagagctt aaaaatggct
     8521 gaaatcactc acaacgatgg atacgctaac aacttggaaa tgaaata
//
