LOCUS       sequence-212582-        8766 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian expression plasmid for BG505 SOSIP.664 fused to a 
            transmembrane tether; QES mutant with enhanced binding to PG16.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 8766)
  AUTHORS   Heredia JD, Park J, Choi H, Gill KS, Procko E
  TITLE     Conformational engineering of HIV-1 Env based on mutational 
            tolerance in the CD4 and PG16 bound states.
  JOURNAL   J Virol. 2019 Mar 20. pii: JVI.00219-19. doi: 10.1128/JVI.00219-19.
  PUBMED    30894475
REFERENCE   2  (bases 1 to 8766)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Virol. 
            2019 Mar 20. pii: JVI.00219-19. doi: 10.1128/JVI.00219-19."
COMMENT     Sequence Label: pCEP4-BG505.SOSIP-QES.i03.c01-TM
FEATURES             Location/Qualifiers
     source          1..8766
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        85..230
                     /label=HSV TK promoter
                     /note="herpes simplex virus thymidine kinase promoter"
     CDS             273..1310
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPDRE
                     MGEAN"
     primer_bind     complement(1308..1327)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    1352..1400
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     enhancer        2054..2433
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2434..2637
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2588..2608
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     2634..2658
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             4690..4707
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     polyA_signal    4853..4987
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4877..4896)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     4931..4950
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(5130..5152)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     rep_origin      5712..7501
                     /label=oriP
                     /note="Epstein-Barr virus oriP replication origin (Yates et
                     al., 2000)"
ORIGIN
        1 ccctgcttca tccccgtggc ccgttgctcg cgtttgctgg cggtgtcccc ggaagaaata
       61 tatttgcatg tctttagttc tatgatgaca caaaccccgc ccagcgtctt gtcattggcg
      121 aattcgaaca cgcagatgca gtcggggcgg cgcggtccga ggtccacttc gcatattaag
      181 gtgacgcgtg tggcctcgaa caccgagcga ccctgcagcg acccgcttaa cagcgtcaac
      241 agcgtgccgc agatcccggg gggcaatgag atatgaaaaa gcctgaactc accgcgacgt
      301 ctgtcgagaa gtttctgatc gaaaagttcg acagcgtctc cgacctgatg cagctctcgg
      361 agggcgaaga atctcgtgct ttcagcttcg atgtaggagg gcgtggatat gtcctgcggg
      421 taaatagctg cgccgatggt ttctacaaag atcgttatgt ttatcggcac tttgcatcgg
      481 ccgcgctccc gattccggaa gtgcttgaca ttggggaatt cagcgagagc ctgacctatt
      541 gcatctcccg ccgtgcacag ggtgtcacgt tgcaagacct gcctgaaacc gaactgcccg
      601 ctgttctgca gccggtcgcg gaggccatgg atgcgatcgc tgcggccgat cttagccaga
      661 cgagcgggtt cggcccattc ggaccgcaag gaatcggtca atacactaca tggcgtgatt
      721 tcatatgcgc gattgctgat ccccatgtgt atcactggca aactgtgatg gacgacaccg
      781 tcagtgcgtc cgtcgcgcag gctctcgatg agctgatgct ttgggccgag gactgccccg
      841 aagtccggca cctcgtgcac gcggatttcg gctccaacaa tgtcctgacg gacaatggcc
      901 gcataacagc ggtcattgac tggagcgagg cgatgttcgg ggattcccaa tacgaggtcg
      961 ccaacatctt cttctggagg ccgtggttgg cttgtatgga gcagcagacg cgctacttcg
     1021 agcggaggca tccggagctt gcaggatcgc cgcggctccg ggcgtatatg ctccgcattg
     1081 gtcttgacca actctatcag agcttggttg acggcaattt cgatgatgca gcttgggcgc
     1141 agggtcgatg cgacgcaatc gtccgatccg gagccgggac tgtcgggcgt acacaaatcg
     1201 cccgcagaag cgcggccgtc tggaccgatg gctgtgtaga agtactcgcc gatagtggaa
     1261 accgacgccc cagcactcgt ccggatcggg agatggggga ggctaactga aacacggaag
     1321 gagacaatac cggaaggaac ccgcgctatg acggcaataa aaagacagaa taaaacgcac
     1381 gggtgttggg tcgtttgttc ataaacgcgg ggttcggtcc cagggctggc actctgtcga
     1441 taccccaccg agaccccatt ggggccaata cgcccgcgtt tcttcctttt ccccacccca
     1501 ccccccaagt tcgggtgaag gcccagggct cgcagccaac gtcggggcgg caggccctgc
     1561 catagccact ggccccgtgg gttagggacg gggtccccca tggggaatgg tttatggttc
     1621 gtgggggtta ttattttggg cgttgcgtgg ggtcaggtcc acgactggac tgagcagaca
     1681 gacccatggt ttttggatgg cctgggcatg gaccgcatgt actggcgcga cacgaacacc
     1741 gggcgtctgt ggctgccaaa cacccccgac ccccaaaaac caccgcgcgg atttctggcg
     1801 tgccaagcta gtcgaccaat tctcatgttt gacagcttat catcgcagat ccgggcaacg
     1861 ttgttgccat tgctgcaggc gcagaactgg taggtatgga agatctatac attgaatcaa
     1921 tattggcaat tagccatatt agtcattggt tatatagcat aaatcaatat tggctattgg
     1981 ccattgcata cgttgtatct atatcataat atgtacattt atattggctc atgtccaata
     2041 tgaccgccat gttgacattg attattgact agttattaat agtaatcaat tacggggtca
     2101 ttagttcata gcccatatat ggagttccgc gttacataac ttacggtaaa tggcccgcct
     2161 ggctgaccgc ccaacgaccc ccgcccattg acgtcaataa tgacgtatgt tcccatagta
     2221 acgccaatag ggactttcca ttgacgtcaa tgggtggagt atttacggta aactgcccac
     2281 ttggcagtac atcaagtgta tcatatgcca agtccgcccc ctattgacgt caatgacggt
     2341 aaatggcccg cctggcatta tgcccagtac atgaccttac gggactttcc tacttggcag
     2401 tacatctacg tattagtcat cgctattacc atggtgatgc ggttttggca gtacaccaat
     2461 gggcgtggat agcggtttga ctcacgggga tttccaagtc tccaccccat tgacgtcaat
     2521 gggagtttgt tttggcacca aaatcaacgg gactttccaa aatgtcgtaa taaccccgcc
     2581 ccgttgacgc aaatgggcgg taggcgtgta cggtgggagg tctatataag cagagctcgt
     2641 ttagtgaacc gtcagatctc tagaagctgg gtaccagctg ctagcgccac catgccaatg
     2701 ggctcccttc agcccttggc taccctgtac cttcttggga tgttggtcgc atctgtgctt
     2761 gccgcagaaa acttgtgggt aactgtctac tatggagtac ccgtttggaa agacgcagaa
     2821 acaacgcttt tttgcgcatc cgacgcgaag gcatacgaaa cggaaaagca caatgtttgg
     2881 gcaacgcatg cctgcgtccc gacagaccca aatcctcagg agattcacct tgagaatgta
     2941 acagaggaat tcaacatgtg gaagaataac atggtagaac aaatgcatac ggatattatc
     3001 agtctttggg accagagcct taaaccttgc gttaaactta cgcccctctg cgtaactctg
     3061 caatgtacta acgtaacgaa taacattacg gacgacatgc gcggcgaact gaagaattgc
     3121 agctttaata tgacaacgga gttgcgagat aaaaagcaga aggtgtacag cctcttctac
     3181 aggcttgacc tcgtgcaaat aaacgaaaac caggggaaca ggagcaataa ctccaataaa
     3241 gaatacagat tgatcaactg taacactagc gagataaccc aggcctgccc gaaggtgtca
     3301 tttgagccga ttcctataca ctattgcgcc ccggcagggt atgcgatctt gaaatgcaag
     3361 gataagaagt ttaatggaac tggtccctgt cccagtgtga gtacggttca gtgtacccac
     3421 ggcataaagc cagtgatgtc cactcagctt ctcctcaatg gcagcctcgc agaggaagag
     3481 gtcatgatac ggagtgagaa tattactaac aatgccaaga atatcttggt ccaatttaat
     3541 acccccgtcc agattaattg tacccgacct aataataata ccagaaaatc cattcgcatc
     3601 ggaccaggtc aggcgttcta tgccacggga gatataatag gtgacatccg acaggcgcat
     3661 tgcaatgtaa gcaaggccac atggaatgag actctgggta aagttgtcaa gcagctcagg
     3721 aagcatttcg ggaacaatac catcatccga tttgcgaaca gtagtggggg tgatcttgaa
     3781 gtaactacac attcatttaa ctgcggcggg gagttttttt attgtaacac atccgggttg
     3841 ttcaactcca cttggatatc taacactagt gttcagggat caaactccac aggatcaaac
     3901 gatagtataa cacttccttg tcgcatcaaa cagattatca acatgtggca gcgaatcggc
     3961 caggcgatgt acgccccgcc tatccaggga gtaatacggt gcgtaagcaa cataacgggc
     4021 cttatactta cgcgcgatgg aggcagcact aattctacca ctgagacgtt ccggcctggc
     4081 ggtggggaca tgcgagacaa ctggcggtct gagttgtaca agtacaaagt ggttaaaatc
     4141 gaacccctgg gtgtcgcccc aacgagatgt aaacgacggg tagtgggaag gaggagaagg
     4201 cgacgggcgg tcggcattgg ggccgttttc ttgggttttc tgggggcggc tgggagcact
     4261 atgggagcag ctagtatgac cctgaccgta caggcgagga acctcctgtc cggtatagtg
     4321 caacaacagt caaaccttct tagggctcct gaggcgcagc aacatctcct taagttgact
     4381 gtctggggca taaagcaact ccaagcgcgg gttctcgcgg tggaaagata ccttagggac
     4441 caacaacttt tggggatgtg gggttgttca ggcaaactca tctgttgcac taacgttcct
     4501 tggaactcct catggtctaa ccgaaatctc tccgagatct gggataatat gacatggttg
     4561 cagtgggata aggagattag caattacact caaataatct atgggctcct ggaggaaagc
     4621 cagaatcagc aggagaagaa tgaacaggat cttctggccc ttgatgggtc aggctctgga
     4681 ggatctggac accatcatca tcaccatggg tccggcgctg gttctgctat ccccatcatg
     4741 ggtatcgttg ctggcctggt tgtccttgca gctgtagtca ctggagctgc ggtcgctgct
     4801 gtgctgtgga gaaagaagag ctcagattag taactcgagg ccggcaaggc cggatccaga
     4861 catgataaga tacattgatg agtttggaca aaccacaact agaatgcagt gaaaaaaatg
     4921 ctttatttgt gaaatttgtg atgctattgc tttatttgta accattataa gctgcaataa
     4981 acaagttaac aacaacaatt gcattcattt tatgtttcag gttcaggggg aggtgtggga
     5041 ggttttttaa agcaagtaaa acctctacaa atgtggtatg gctgattatg atccggctgc
     5101 ctcgcgcgtt tcggtgatga cggtgaaaac ctctgacaca tgcagctccc ggagacggtc
     5161 acagcttgtc tgtaagcgga tgccgggagc agacaagccc gtcagggcgc gtcagcgggt
     5221 gttggcgggt gtcggggcgc agccatgagg tcgactctag aggatcgatc cccgccccgg
     5281 acgaactaaa cctgactacg acatctctgc cccttcttcg cggggcagtg catgtaatcc
     5341 cttcagttgg ttggtacaac ttgccaactg ggccctgttc cacatgtgac acgggggggg
     5401 accaaacaca aaggggttct ctgactgtag ttgacatcct tataaatgga tgtgcacatt
     5461 tgccaacact gagtggcttt catcctggag cagactttgc agtctgtgga ctgcaacaca
     5521 acattgcctt tatgtgtaac tcttggctga agctcttaca ccaatgctgg gggacatgta
     5581 cctcccaggg gcccaggaag actacgggag gctacaccaa cgtcaatcag aggggcctgt
     5641 gtagctaccg ataagcggac cctcaagagg gcattagcaa tagtgtttat aaggccccct
     5701 tgttaaccct aaacgggtag catatgcttc ccgggtagta gtatatacta tccagactaa
     5761 ccctaattca atagcatatg ttacccaacg ggaagcatat gctatcgaat tagggttagt
     5821 aaaagggtcc taaggaacag cgatatctcc caccccatga gctgtcacgg ttttatttac
     5881 atggggtcag gattccacga gggtagtgaa ccattttagt cacaagggca gtggctgaag
     5941 atcaaggagc gggcagtgaa ctctcctgaa tcttcgcctg cttcttcatt ctccttcgtt
     6001 tagctaatag aataactgct gagttgtgaa cagtaaggtg tatgtgaggt gctcgaaaac
     6061 aaggtttcag gtgacgcccc cagaataaaa tttggacggg gggttcagtg gtggcattgt
     6121 gctatgacac caatataacc ctcacaaacc ccttgggcaa taaatactag tgtaggaatg
     6181 aaacattctg aatatcttta acaatagaaa tccatggggt ggggacaagc cgtaaagact
     6241 ggatgtccat ctcacacgaa tttatggcta tgggcaacac ataatcctag tgcaatatga
     6301 tactggggtt attaagatgt gtcccaggca gggaccaaga caggtgaacc atgttgttac
     6361 actctatttg taacaagggg aaagagagtg gacgccgaca gcagcggact ccactggttg
     6421 tctctaacac ccccgaaaat taaacggggc tccacgccaa tggggcccat aaacaaagac
     6481 aagtggccac tctttttttt gaaattgtgg agtgggggca cgcgtcagcc cccacacgcc
     6541 gccctgcggt tttggactgt aaaataaggg tgtaataact tggctgattg taaccccgct
     6601 aaccactgcg gtcaaaccac ttgcccacaa aaccactaat ggcaccccgg ggaatacctg
     6661 cataagtagg tgggcgggcc aagatagggg cgcgattgct gcgatctgga ggacaaatta
     6721 cacacacttg cgcctgagcg ccaagcacag ggttgttggt cctcatattc acgaggtcgc
     6781 tgagagcacg gtgggctaat gttgccatgg gtagcatata ctacccaaat atctggatag
     6841 catatgctat cctaatctat atctgggtag cataggctat cctaatctat atctgggtag
     6901 catatgctat cctaatctat atctgggtag tatatgctat cctaatttat atctgggtag
     6961 cataggctat cctaatctat atctgggtag catatgctat cctaatctat atctgggtag
     7021 tatatgctat cctaatctgt atccgggtag catatgctat cctaatagag attagggtag
     7081 tatatgctat cctaatttat atctgggtag catatactac ccaaatatct ggatagcata
     7141 tgctatccta atctatatct gggtagcata tgctatccta atctatatct gggtagcata
     7201 ggctatccta atctatatct gggtagcata tgctatccta atctatatct gggtagtata
     7261 tgctatccta atttatatct gggtagcata ggctatccta atctatatct gggtagcata
     7321 tgctatccta atctatatct gggtagtata tgctatccta atctgtatcc gggtagcata
     7381 tgctatcctc atgcatatac agtcagcata tgatacccag tagtagagtg ggagtgctat
     7441 cctttgcata tgccgccacc tcccaagggg gcgtgaattt tcgctgcttg tccttttcct
     7501 gctggttgct cccattctta ggtgaattta aggaggccag gctaaagccg tcgcatgtct
     7561 gattgctcac caggtaaatg tcgctaatgt tttccaacgc gagaaggtgt tgagcgcgga
     7621 gctgagtgac gtgacaacat gggtatgccc aattgcccca tgttgggagg acgaaaatgg
     7681 tgacaagaca gatggccaga aatacaccaa cagcacgcat gatgtctact ggggatttat
     7741 tctttagtgc gggggaatac acggctttta atacgattga gggcgtctcc taacaagtta
     7801 catcactcct gcccttcctc accctcatct ccatcacctc cttcatctcc gtcatctccg
     7861 tcatcaccct ccgcggcagc cccttccacc ataggtggaa accagggagg caaatctact
     7921 ccatcgtcaa agctgcacac agtcaccctg atattgcagg taggagcggg ctttgtcata
     7981 acaaggtcct taatcgcatc cttcaaaacc tcagcaaata tatgagtttg taaaaagacc
     8041 atgaaataac agacaatgga ctcccttagc gggccaggtt gtgggccggg tccaggggcc
     8101 attccaaagg ggagacgact caatggtgta agacgacatt gtggaatagc aagggcagtt
     8161 cctcgcctta ggttgtaaag ggaggtctta ctacctccat atacgaacac accggcgacc
     8221 caagttcctt cgtcggtagt cctttctacg tgactcctag ccaggagagc tcttaaacct
     8281 tctgcaatgt tctcaaattt cgggttggaa cctccttgac cacgatgctt tccaaaccac
     8341 cctccttttt tgcgcctgcc tccatcaccc tgaccccggg gtccagtgct tgggccttct
     8401 cctgggtcat ctgcggggcc ctgctctatc gctcccgggg gcacgtcagg ctcaccatct
     8461 gggccacctt cttggtggta ttcaaaataa tcggcttccc ctacagggtg gaaaaatggc
     8521 cttctacctg gagggggcct gcgcggtgga gacccggatg atgatgactg actactggga
     8581 ctcctgggcc tcttttctcc acgtccacga cctctccccc tggctctttc acgacttccc
     8641 cccctggctc tttcacgtcc tctaccccgg cggcctccac tacctcctcg accccggcct
     8701 ccactacctc ctcgaccccg gcctccactg cctcctcgac cccggcctcc acctcctgct
     8761 cctgcc
//
