LOCUS       sequence-212583-        8667 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses the C. elegans acy-1 cDNA pan-neuronally.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8667)
  AUTHORS   Reynolds NK, Schade MA, Miller KG
  TITLE     Convergent, RIC-8-dependent Galpha signaling pathways in the 
            Caenorhabditis elegans synaptic signaling network.
  JOURNAL   Genetics. 2005 Feb;169(2):651-70. doi: 10.1534/genetics.104.031286. 
            Epub 2004 Oct 16.
  PUBMED    15489511
REFERENCE   2  (bases 1 to 8667)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1534/genetics.104.031286"; journalName: "Genetics"; date: 
            "2005-02"; volume: "169"; issue: "2"; pages: "651-70"
COMMENT     Sequence Label: KG#62
FEATURES             Location/Qualifiers
     source          1..8667
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(6130..6152)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     6252..6271
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(6349..6367)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        6435..6539
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6540..6608,6609..7400)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6758..6777)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7571..8159
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8060..8079
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8313..8330
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    8447..8468
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(8483..8488,8489..8506,8507..8513)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8521..8537
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     8526..8548
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     8545..8561
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     8545..8561
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 cgctaacaac ttggaaatga aataagcttg ggctgcagga tcttcagatg ggagcagtgg
       61 actgtctatt tgatattctt aaaatgtact ctataaaaga tgaaactgta atgaccaatt
      121 ccctacataa atatttttaa ctaattggtc ggatatttgg ggatcagaga agtacttgtt
      181 tttgcgatat taataatgat atatttttct agtaaaaaaa tgttaagcga tttgtgaata
      241 attaagttgg cttgaaaaag attttttgat agctatgtat tttgttaatt taaatcagat
      301 tttaaaactt tattattgac tatttatata tattatatat tatatatttt catggtttaa
      361 tcaaaatcta ttaaattatt tctaaaatct ctaaatatcg aattctctaa aaagcttcat
      421 caacaaaagc atttcaatta tctaacacaa tgagaataat tcaagaactg aaatagaaag
      481 aaatctagaa aaaaaagaga gtgaggtgcc ctattaaatc ggtgctctcc tctctaacag
      541 aagcgcctac acgcacccaa gagagctctt tttctttctt ttttcggagc acacatacac
      601 gcatacactc ctcagaagtg tatagagaaa atgggagaag aagcgaaaga agacgaagaa
      661 gcggttgtga tgtatgcatc gggcgtttct atctctctcc gtgagcaacg agctagtcaa
      721 cccatcgatc catttttgtg agcacacaca gagagagact caaacacact cacagcttca
      781 cactgagctc caagagctct gtagagagaa gccagagtat gctttcctcg attttcctaa
      841 cgtcttcttc ttctgctttt ttctttttcc caacgagttg tagtccacta acttcaattt
      901 ttcctccaga gaaagagttt tagagagaca gctgataaat acactagaga aataaaaaga
      961 atgataaaaa tttgctattt taatcagttc cctctcgttt ctctaagttt tgttgttggg
     1021 ttgggaaatt aaaattggaa aatggaaata acgttttttt ctgtctaatc ttgaattctc
     1081 cagacatttc ttatttcctt ttttgttgca agtttttctt ccttctctaa tttttaatca
     1141 aagatatctc gctatgaact ttgaattttt gatattttga ttctataaat acataaagta
     1201 atctaaatgc tcttttaaaa taaatctaca gtagccctat tttcagggat ccccgggatt
     1261 ggccaaagga cccaaaggta tgtttcgaat gatactaaca taacatagaa cattttcagg
     1321 aggacccttg gctagcgtcg acggtaccgg taaaaatgga cgacgatgtc ggtgaacgaa
     1381 caccggctct cggtggttca tgtggaccat cagttcgtgc acacagtagt tccccaagac
     1441 gtgttccatt atttgagaga gcatctgcta gatggtggaa tcctcaattt cgatctgcaa
     1501 cactagaagc tcaatattgg aagtgtagtt tcagtcagtt aagagatcga tttcgttctg
     1561 gattaatata tattgccgtt gtaattgccg cttggacact ttatttagct ttattcgata
     1621 gaacttttat tcagcattgg atcgtttcac tatgcctctg tgccattatt ttcgcgatgt
     1681 ttgccttcac ggcgtgcgcc gctcaatacc aaaggttcta catgccaacc tcatttctat
     1741 gcactttttt gatatgcctt gtcacgttgc tcatcttctc cgcagagaat caagctgcgt
     1801 ttatgactcc agttgcaagt cttgccacaa gttttcaggt cgttctcctg atctacacag
     1861 tcattccact gccactctat ttatgtatac tcatcggaat aatctactca atcctatttg
     1921 agattctcaa caagaacaaa attggtcttg aagaggccgg atacattaag cttgtccttc
     1981 acgctggtgt ccatttactc ggtgtccatt tgttcatttt aactcaagtg cgacagagga
     2041 aaacgtttct gaaagtggga caatcgatgt tggcaagaaa ggatctagag ttggagacac
     2101 agtttaaaga tcatatgatt cagtctgtga tgcctaaaaa ggtagctgat gaattactga
     2161 aagatgcatc agaacttcgc cgaccttctg cttccaacga ctcaaactgc cgaacttcaa
     2221 atgctacaca agttgaccag ccccttgcca agatggtacc tgaataccga aagttccgtc
     2281 catttacgat gaatctcatg acaaatgtgt cgattctgtt tgcggatatt gctgggttta
     2341 ctaaaatgtc atcaaataaa tctgccgacg aacttgtcaa tctgctcaat gatctttttg
     2401 gaagattcga tactttgtgt cgattgagag gattagagaa gatttcaact ttgggagatt
     2461 gttactattg tgttgctgga tgtcctgaac catgtgatga tcatgcatgc agaacagtcg
     2521 aaatgggtct cgacatgatt gttgcaatcc gccaatttga tattgatcgt ggacaagaag
     2581 tgaatatgcg tgtgggaatt catacaggaa aagtaatgtg cggaatggtt ggaacaaaaa
     2641 gattcaaatt cgatgtgttc tcaaacgatg tcacactggc aaatgagatg gaatcttctg
     2701 gtgttgctgg aagagttcac gtttccgaag caactgcaaa acttttgaaa gggctttatg
     2761 aaattgaaga gggaccggat tatgatggtc cattgaggat gcaagttcag ggaactgaac
     2821 gtcgtgttaa accagaaagc atgaagactt tctttatcaa aggacgtatt aatgatggtg
     2881 tagaagaaga agtgatgcaa gttcaggaag ttgagagcct tcacagtcag aaaagcagta
     2941 aaaagtcgac tctaaaacag aaatgggcgg aaaagttgaa aatgaaccac acaaattctt
     3001 atccaatgcg agccgctgca cgtgaaggag gtggaagttt gagaataaaa ttggcagaga
     3061 gaaatagaag tacacagtta ctgccaaaag aatcaaattc aatttgtata atggaagata
     3121 accgaaagtc agcatcactt caagcattgg caacgaataa ttttaatgga agtaatacag
     3181 atacaaataa cacttatagt gaacgtggag ttgctggaag tgttagtaag aagtctgtag
     3241 ctggaagtga aagtaatagt ataaaaggaa gtagaagcag tggactacaa ttatctctac
     3301 aagatggaaa ttctgattta aattcagttg gaggtttgga tactgcaatc agtcaccatc
     3361 acaatgcagc atctctcact cgatttgata ctgataacaa ttttgatcag cgtttggcaa
     3421 tggtaattgg acaaggagaa ggaggatttg ataaaggatt ttggaatcat catgatagtt
     3481 tgaataagtg gactcttcga tttaatgaaa aggacgttga agaggaatat cgagctcatt
     3541 ttgtggattc atcggaaaga tatacggcat caaagaaagg tcatgttgaa agacataaag
     3601 atttgatgga acaaggtgga gaaaaagatg gaattactgg atcaacggtc aacaagtata
     3661 gatattcagg agtcttcatc gatataattg ttgccacgtt gatatttgta atatcgggtg
     3721 cagttgcaat tatgtctgtc cgtccattcc cattatcact cattgcatac ttcccatttg
     3781 ctgctgcaat tctcattctt acaattgtcc ttattggtct tccacttctc gcccgtaaaa
     3841 aatcattcca atgtgcaaat caatggatgc cacgtcatct catcggtctt cttctgatct
     3901 ttcttccaat cggtgtcgca atctgtatca tgccattgtg ccaaagtgga gactgtgcaa
     3961 atgtaattct gaactaccgt cttgcattca gttatgtcac cattcttgcc atattcgctc
     4021 attgcaactt ctcccaatta gctgcctggc caaaaactac tgctgcagta tttatcggac
     4081 ttctccacat tgctggagtt ttctactgcg aattcaattt gaaacactta gttgaagagc
     4141 aagacacctg caacgtgaca gcaatcatga ttccaccaat tcgcaaaggt ctgaactaca
     4201 caattgcatt gaactcgaca tctgcaagaa cattatctca agattttgga tctccattat
     4261 tcatttggga gctgcttctg gatgtgatcc tgtcgattgt tctagttgca ttccttaatt
     4321 atcagttcga aaccgcattc cgtatgtcat tctttggaga tgttcaagct cgtcgggaca
     4381 ctgaaagaat gcaaatcgtg cgtgatcaag ccgattggct cctgaataac gtgatcccag
     4441 cccacgcagt agaaagtctt aaaacggata ccaaatacag tgaaaaccat gaaacagttg
     4501 gtgtactgtt cgcttcaatc actaattgga atgatatgta tgaagagaat ttcgaagggg
     4561 gcagagaatt cttgagagtt ctcaatgaag ttattggtga ctttgatgag cttctcgatc
     4621 ggccggactt cacgcatatt gaaaaaatca aaacaattgg gcctgcgtat atggcagcaa
     4681 gtggattgaa tccagaacga aaaaagaata tgttgcatcc aaaagagcat ctatatcaga
     4741 tggttgattt tgcattggca gttcaacatg tactcagtgt tttcaacgag gatttgctca
     4801 attttgattt tgtttgcaag ttaggactta atattggacc cgtcactgct ggtgtcattg
     4861 gaacaacaaa gttgtactac gatatctggg gtgacactgt aaacattgct agccgaatgt
     4921 acagcaccgg agttttaaat cgcattcaag tatcacaaca cacacgagaa tatctcctgg
     4981 atcggtatga attcgagttc cgcgatcata ttgaagtcaa gggaattgat ggaggaatgg
     5041 atacttatct tttggttggt cgaaaaggtg atggaatacc tccatcaatc aaggataacc
     5101 aagaagacga attttaaatc tcgaggagct cccgagatct tcagcgctca gcattcgtag
     5161 aattgtaagt ttaaactttc tcatactaac taacgcgctc tatttaaatt ttcagaattc
     5221 caactgagcg ccggtcgcta ccattaccaa cttgtctggt gtcaaaaata ataggggccg
     5281 ctgtcatcag agtaagttta aactgagttc tactaactaa cgagtaatat ttaaattttc
     5341 agcatctcgc gcccgtgcct ctgacttcta agtccaatta ctcttcaaca tccctacatg
     5401 ctctttctcc ctgtgctccc accccctatt tttgttatta tcaaaaaact tctcttaatt
     5461 tctttgtttt ttagcttctt ttaagtcacc tctaacaatg aaattgtgta gattcaaaaa
     5521 tagaattaat tcgtaataaa aagtcgaaaa aaattgtgct ccctcccccc attaataata
     5581 attctatccc aaaatctaca caatgttctg tgtacacttc ttatgttttt tacttctgat
     5641 aaattttttt gaaacatcat agaaaaaacc gcacacaaaa taccttatca tatgttacgt
     5701 ttcagtttat gaccgcaatt tttatttctt cgcacgtctg ggcctctcat gacgtcaaat
     5761 catgctcatc gtgaaaaagt tttggagtat ttttggaatt tttcaatcaa gtgaaagttt
     5821 atgaaattaa ttttcctgct tttgcttttt ggggtttccc ctattgtttg tcaagatttc
     5881 gaggacggcg tttttcttgc taaaatcaca agtattgatg agcacgatgc aagaaagatc
     5941 ggaagaaggt ttgggtttga ggctcagtgg aaggtgagta gaagttgata atttgaaagt
     6001 ggagtagtgt ctatggggtt tttgccttaa atgacagaat acattcccaa tataccaaac
     6061 ataactgttt cctactagtc ggccgtacgg gccctttcgt ctcgcgcgtt tcggtgatga
     6121 cggtgaaaac ctctgacaca tgcagctccc ggagacggtc acagcttgtc tgtaagcgga
     6181 tgccgggagc agacaagccc gtcagggcgc gtcagcgggt gttggcgggt gtcggggctg
     6241 gcttaactat gcggcatcag agcagattgt actgagagtg caccatatgc ggtgtgaaat
     6301 accgcacaga tgcgtaagga gaaaataccg catcaggcgg ccttaagggc ctcgtgatac
     6361 gcctattttt ataggttaat gtcatgataa taatggtttc ttagacgtca ggtggcactt
     6421 ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt
     6481 atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta
     6541 tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg
     6601 tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac
     6661 gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg
     6721 aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc
     6781 gtattgacgc cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg
     6841 ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat
     6901 gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg
     6961 gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg
     7021 atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc
     7081 ctgtagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt
     7141 cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct
     7201 cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc
     7261 gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca
     7321 cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct
     7381 cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt
     7441 taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga
     7501 ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca
     7561 aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac
     7621 caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg
     7681 taactggctt cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag
     7741 gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac
     7801 cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt
     7861 taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg
     7921 agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc
     7981 ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc
     8041 gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc
     8101 acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa
     8161 acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt
     8221 tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg
     8281 ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag
     8341 agcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc
     8401 acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc
     8461 tcactcatta ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa
     8521 ttgtgagcgg ataacaattt cacacaggaa acagctatga ccatgattac gccaagctgt
     8581 aagtttaaac atgatcttac taactaacta ttctcattta aattttcaga gcttaaaaat
     8641 ggctgaaatc actcacaacg atggata
//
