LOCUS       sequence-212584-        8497 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector to express wild-type N terminal HTT gene with 
            short 3'UTR in mammalian cells (encodes Myc-tagged wild type 
            huntingtin exon1 fragment with 23 repeats of Q).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8497)
  AUTHORS   Xu H, An JJ, Xu B
  TITLE     Distinct cellular toxicity of two mutant huntingtin mRNA variants 
            due to translation regulation.
  JOURNAL   PLoS One. 2017 May 11;12(5):e0177610. doi: 
            10.1371/journal.pone.0177610. eCollection 2017.
  PUBMED    28494017
REFERENCE   2  (bases 1 to 8497)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2017-05-11"; volume: "12(5):e0177610. doi"; pages: " 
            10.1371/journal.pone.0177610. eCollection 2017"
COMMENT     Sequence Label: Ultra-Exon1Q23-Myc-A
FEATURES             Location/Qualifiers
     source          1..8497
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..227
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             228..408
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    455..580
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1073..1306
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1491..1535
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    1833..1950
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2015..3226
                     /label=UbC promoter
                     /note="human ubiquitin C promoter"
     primer_bind     3063..3082
                     /label=hUBCpro-F
                     /note="Human Ubiquitin C (UbC) promoter, forward primer"
     CDS             3596..3625
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             3641..3658
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     misc_feature    4337..4925
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(4390..4410)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4808..4819)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             5016..5249
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    5321..5442
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5358..5377)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5412..5431
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      5482..5617
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(5637..5656)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(5638..5656)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(5666..5683)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(5666..5682)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5675..5697)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      5824..6279
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5911..5930)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6121..6142
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        6305..6409
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6410..6478,6479..7270)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6628..6647)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7441..8029
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7930..7949
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8183..8200
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    8317..8338
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(8353..8358,8359..8376,8377..8383)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8391..8407
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     8396..8418
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     8415..8431
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     8415..8431
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     8450..8470
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        8452..8470
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 tgtagtctta tgcaatactc ttgtagtctt gcaacatggt aacgatgagt tagcaacatg
       61 ccttacaagg agagaaaaag caccgtgcat gccgattggt ggaagtaagg tggtacgatc
      121 gtgccttatt aggaaggcaa cagacgggtc tgacatggat tggacgaacc actgaattgc
      181 cgcattgcag agatattgta tttaagtgcc tagctcgata cataaacggg tctctctggt
      241 tagaccagat ctgagcctgg gagctctctg gctaactagg gaacccactg cttaagcctc
      301 aataaagctt gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt gactctggta
      361 actagagatc cctcagaccc ttttagtcag tgtggaaaat ctctagcagt ggcgcccgaa
      421 cagggacttg aaagcgaaag ggaaaccaga ggagctctct cgacgcagga ctcggcttgc
      481 tgaagcgcgc acggcaagag gcgaggggcg gcgactggtg agtacgccaa aaattttgac
      541 tagcggaggc tagaaggaga gagatgggtg cgagagcgtc agtattaagc gggggagaat
      601 tagatcgcga tgggaaaaaa ttcggttaag gccaggggga aagaaaaaat ataaattaaa
      661 acatatagta tgggcaagca gggagctaga acgattcgca gttaatcctg gcctgttaga
      721 aacatcagaa ggctgtagac aaatactggg acagctacaa ccatcccttc agacaggatc
      781 agaagaactt agatcattat ataatacagt agcaaccctc tattgtgtgc atcaaaggat
      841 agagataaaa gacaccaagg aagctttaga caagatagag gaagagcaaa acaaaagtaa
      901 gaccaccgca cagcaagcgg ccgctgatct tcagacctgg aggaggagat atgagggaca
      961 attggagaag tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac
     1021 ccaccaaggc aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt
     1081 tgttccttgg gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga
     1141 cggtacaggc cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg
     1201 ctattgaggc gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg
     1261 caagaatcct ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt
     1321 gctctggaaa actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat
     1381 ctctggaaca gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt
     1441 acacaagctt aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac
     1501 aagaattatt ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt
     1561 ggctgtggta tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag
     1621 tttttgctgt actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc
     1681 agacccacct cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg
     1741 gagagagaga cagagacaga tccattcgat tagtgaacgg atcggcactg cgtgcgccaa
     1801 ttctgcagac aaatggcagt attcatccac aattttaaaa gaaaaggggg gattgggggg
     1861 tacagtgcag gggaaagaat agtagacata atagcaacag acatacaaac taaagaatta
     1921 caaaaacaaa ttacaaaaat tcaaaatttt cgggtttatt acagggacag cagagatcca
     1981 gtttggttaa ttaacccgtg tcggctccag atctggcctc cgcgccgggt tttggcgcct
     2041 cccgcgggcg cccccctcct cacggcgagc gctgccacgt cagacgaagg gcgcagcgag
     2101 cgtcctgatc cttccgcccg gacgctcagg acagcggccc gctgctcata agactcggcc
     2161 ttagaacccc agtatcagca gaaggacatt ttaggacggg acttgggtga ctctagggca
     2221 ctggttttct ttccagagag cggaacaggc gaggaaaagt agtcccttct cggcgattct
     2281 gcggagggat ctccgtgggg cggtgaacgc cgatgattat ataaggacgc gccgggtgtg
     2341 gcacagctag ttccgtcgca gccgggattt gggtcgcggt tcttgtttgt ggatcgctgt
     2401 gatcgtcact tggtgagtag cgggctgctg ggctggccgg ggctttcgtg gccgccgggc
     2461 cgctcggtgg gacggaagcg tgtggagaga ccgccaaggg ctgtagtctg ggtccgcgag
     2521 caaggttgcc ctgaactggg ggttgggggg agcgcagcaa aatggcggct gttcccgagt
     2581 cttgaatgga agacgcttgt gaggcgggct gtgaggtcgt tgaaacaagg tggggggcat
     2641 ggtgggcggc aagaacccaa ggtcttgagg ccttcgctaa tgcgggaaag ctcttattcg
     2701 ggtgagatgg gctggggcac catctgggga ccctgacgtg aagtttgtca ctgactggag
     2761 aactcggttt gtcgtctgtt gcgggggcgg cagttatggc ggtgccgttg ggcagtgcac
     2821 ccgtaccttt gggagcgcgc gccctcgtcg tgtcgtgacg tcacccgttc tgttggctta
     2881 taatgcaggg tggggccacc tgccggtagg tgtgcggtag gcttttctcc gtcgcaggac
     2941 gcagggttcg ggcctagggt aggctctcct gaatcgacag gcgccggacc tctggtgagg
     3001 ggagggataa gtgaggcgtc agtttctttg gtcggtttta tgtacctatc ttcttaagta
     3061 gctgaagctc cggttttgaa ctatgcgctc ggggttggcg agtgtgtttt gtgaagtttt
     3121 ttaggcacct tttgaaatgt aatcatttgg gtcaatatgt aattttcagt gttagactag
     3181 taaattgtcc gctaaattct ggccgttttt ggcttttttg ttagacgaag cttgggctgc
     3241 aggtccgatc caccgggatc cactagtcca gtgtggtgga attctgcaga taaacaccac
     3301 catggcgacc ctggaaaagc tgatgaaggc cttcgagtcc ctcaagtcct tccagcagca
     3361 gcagcagcaa cagcagcaac agcagcagca acaacagcag cagcagcaac aacaacagca
     3421 gccgccaccg ccgccgccgc cgccgccgcc tcctcagctt cctcagccgc cgccgcaggc
     3481 acagccgctg ctgcctcagc cgcagccgcc cccgccgccg cccccgccgc cacccggccc
     3541 ggctgtggct gaggagccgc tgcaccgacc agcggccgct cgaggtcacc cattcgaaca
     3601 aaaactcatc tcagaagagg atctgaatat gcataccggt catcatcacc atcaccattg
     3661 agtttccggg ccttctagag gatccgcgcc atggtgggag agactgtgag gcggcagctg
     3721 gggccggagc ctttggaagt ctgcgccctt gtgccctgcc tccaccgagc cagcttggtc
     3781 cctatgggct tccgcacatg ccgcgggcgg ccaggcaacg tgcgtgtctc tgccatgtgg
     3841 cagaagtgct ctttgtggca gtggccaggc agggagtgtc tgcagtcctg gtggggctga
     3901 gcctgaggcc ttccagaaag caggagcagc tgtgctgcac cccatgtggg tgatcaggtc
     3961 ctttctcctg atagtcacct gctggttgtt gccaggttac agctgctctt gcatctgggc
     4021 cagaagtcct ccctcctgca ggctgggtgt tggcccccct gctgtcctgc agtagaaggt
     4081 gccgtgagca ggctttggga acactggcct gggtctccct ggtggggtgt gcatgccacg
     4141 ccccgtgtct ggatgcacag atgccatggc ctgtgctggg ccagtggctg ggggtgctag
     4201 acacccggca ccattctccc ttctctcttt tcttctcagg atttaaaatt taattatatc
     4261 agtaaagaga ttaattttaa cgtaactctt tctatgcctg tgtaaagtat gtgaatcgca
     4321 aggccgtcga ctcgacaatc aacctctgga ttacaaaatt tgtgaaagat tgactggtat
     4381 tcttaactat gttgctcctt ttacgctatg tggatacgct gctttaatgc ctttgtatca
     4441 tgctattgct tcccgtatgg ctttcatttt ctcctccttg tataaatcct ggttgctgtc
     4501 tctttatgag gagttgtggc ccgttgtcag gcaacgtggc gtggtgtgca ctgtgtttgc
     4561 tgacgcaacc cccactggtt ggggcattgc caccacctgt cagctccttt ccgggacttt
     4621 cgctttcccc ctccctattg ccacggcgga actcatcgcc gcctgccttg cccgctgctg
     4681 gacaggggct cggctgttgg gcactgacaa ttccgtggtg ttgtcgggga agctgacgtc
     4741 ctttccatgg ctgctcgcct gtgttgccac ctggattctg cgcgggacgt ccttctgcta
     4801 cgtcccttcg gccctcaatc cagcggacct tccttcccgc ggcctgctgc cggctctgcg
     4861 gcctcttccg catcttcgcc ttcgccctca gacgagtcgg atctcccttt gggccgcctc
     4921 cccgcctgga attaattcga gctcggtacc tttaagacca atgacttaca aggcagctgt
     4981 agatcttagc cactttttaa aagaaaaggg gggactggaa gggctacgta actcccaacg
     5041 aagacaagat ctgctttttg cttgtactgg gtctctctgg ttagaccaga tctgagcctg
     5101 ggagctctct ggctaactag ggaacccact gcttaagcct caataaagct tgccttgagt
     5161 gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt aactagagat ccctcagacc
     5221 cttttagtca gtgtggaaaa tctctagcag tagtagttca tgtcatctta ttattcagta
     5281 tttataactt gcaaagaaat gaatatcaga gagtgagagg aacttgttta ttgcagctta
     5341 taatggttac aaataaagca atagcatcac aaatttcaca aataaagcat ttttttcact
     5401 gcattctagt tgtggtttgt ccaaactcat caatgtatct tatcatgtct ggctctagct
     5461 atcccgcccc taactccgcc catcccgccc ctaactccgc ccagttccgc ccattctccg
     5521 ccccatggct gactaatttt ttttatttat gcagaggccg aggccgcctc ggcctctgag
     5581 ctattccaga agtagtgagg aggctttttt ggaggcctag ggacgtaccc aattcgccct
     5641 atagtgagtc gtattacgcg cgctcactgg ccgtcgtttt acaacgtcgt gactgggaaa
     5701 accctggcgt tacccaactt aatcgccttg cagcacatcc ccctttcgcc agctggcgta
     5761 atagcgaaga ggcccgcacc gatcgccctt cccaacagtt gcgcagcctg aatggcgaat
     5821 gggacgcgcc ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga
     5881 ccgctacact tgccagcgcc ctagcgcccg ctcctttcgc tttcttccct tcctttctcg
     5941 ccacgttcgc cggctttccc cgtcaagctc taaatcgggg gctcccttta gggttccgat
     6001 ttagtgcttt acggcacctc gaccccaaaa aacttgatta gggtgatggt tcacgtagtg
     6061 ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg ttctttaata
     6121 gtggactctt gttccaaact ggaacaacac tcaaccctat ctcggtctat tcttttgatt
     6181 tataagggat tttgccgatt tcggcctatt ggttaaaaaa tgagctgatt taacaaaaat
     6241 ttaacgcgaa ttttaacaaa atattaacgc ttacaattta ggtggcactt ttcggggaaa
     6301 tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat
     6361 gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca
     6421 acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca
     6481 cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta
     6541 catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt
     6601 tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc gtattgacgc
     6661 cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc
     6721 accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc
     6781 cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa
     6841 ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga
     6901 accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgtagcaat
     6961 ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca
     7021 attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc
     7081 ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc gcggtatcat
     7141 tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag
     7201 tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa
     7261 gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca
     7321 tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc
     7381 ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc
     7441 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
     7501 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
     7561 cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt
     7621 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
     7681 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
     7741 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
     7801 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
     7861 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
     7921 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctcact
     7981 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
     8041 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc
     8101 gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg
     8161 ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcccaat
     8221 acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc acgacaggtt
     8281 tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc tcactcatta
     8341 ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa ttgtgagcgg
     8401 ataacaattt cacacaggaa acagctatga ccatgattac gccaagcgcg caattaaccc
     8461 tcactaaagg gaacaaaagc tggagctgca agcttaa
//
