LOCUS       sequence-212585-        7075 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bicistronic lentivirus expressing Cxcl5 and Cre.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 7075)
  AUTHORS   Mollaoglu G, Jones A, Wait SJ, Mukhopadhyay A, Jeong S, Arya R, 
            Camolotto SA, Mosbruger TL, Stubben CJ, Conley CJ, Bhutkar A, 
            Vahrenkamp JM, Berrett KC, Cessna MH, Lane TE, Witt BL, Salama ME, 
            Gertz J, Jones KB, Snyder EL, Oliver TG
  TITLE     The Lineage-Defining Transcription Factors SOX2 and NKX2-1 Determine
            Lung Cancer Cell Fate and Shape the Tumor Immune Microenvironment.
  JOURNAL   Immunity. 2018 Oct 16;49(4):764-779.e9. doi: 
            10.1016/j.immuni.2018.09.020.
  PUBMED    30332632
REFERENCE   2  (bases 1 to 7075)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.immuni.2018.09"; journalName: "Immunity"; date: 
            "2018-10-16- 16"; volume: "49"; issue: "4"; pages: "764-779.e9"
COMMENT     Sequence Label: ACTIN-Cxcl5-PGK-Cre
FEATURES             Location/Qualifiers
     source          1..7075
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(47..66)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        238..617
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        619..894
                     /label=chicken beta-actin promoter
     primer_bind     1835..1857
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     promoter        2356..2855
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(2391..2414)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     2788..2807
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             2859..2879
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             2880..3908
                     /codon_start=1
                     /product="site-specific recombinase"
                     /label=Cre
                     /note="Cre is a site-specific recombinase from 
                     bacteriophage P1. Recombination occurs at loxP sequences."
                     /translation="SNLLTVHQNLPALPVDATSDEVRKNLMDMFRDRQAFSEHTWKMLL
                     SVCRSWAAWCKLNNRKWFPAEPEDVRDYLLYLQARGLAVKTIQQHLGQLNMLHRRSGLP
                     RPSDSNAVSLVMRRIRKENVDAGERAKQALAFERTDFDQVRSLMENSDRCQDIRNLAFL
                     GIAYNTLLRIAEIARIRVKDISRTDGGRMLIHIGRTKTLVSTAGVEKALSLGVTKLVER
                     WISVSGVADDPNNYLFCRVRKNGVAAPSATSQLSTRALEGIFEATHRLIYGAKDDSGQR
                     YLAWSGHSARVGAARDMARAGVSIPEIMQAGGWTNVNIVMNYIRNLDSETGAMVRLLED
                     GD"
     misc_feature    3964..4552
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(4017..4037)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4435..4446)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(4555..4571)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4556..4572)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             5081..5261
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     rep_origin      complement(5323..5911)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5403..5422)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(6082..6873,6874..6942))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     6705..6724
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(6943..7047)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gtcgacggat cgggagatct cccgatcccc tatggtgcac tctcagtaca atctgctctg
       61 atgccgcata gttaagccag tatctgctcc ctgcttgtgt gttggaggtc gctgagtagt
      121 gcgcgagcaa aatttaagct acaacaaggc aaggcttgac cgacaattgc atgaagaatc
      181 tgcttagggt taggcgtttt gcgctgcttc gcgatgtacg ggccagatat acgcgttgac
      241 attgattatt gactagttat taatagtaat caattacggg gtcattagtt catagcccat
      301 atatggagtt ccgcgttaca taacttacgg taaatggccc gcctggctga ccgcccaacg
      361 acccccgccc attgacgtca ataatgacgt atgttcccat agtaacgcca atagggactt
      421 tccattgacg tcaatgggtg gagtatttac ggtaaactgc ccacttggca gtacatcaag
      481 tgtatcatat gccaagtacg ccccctattg acgtcaatga cggtaaatgg cccgcctggc
      541 attatgccca gtacatgacc ttatgggact ttcctacttg gcagtacatc tacgtattag
      601 tcatcgctat taacatggtc gaggtgagcc ccacgttctg cttcactctc cccatctccc
      661 ccccctcccc acccccaatt ttgtatttat ttatttttta attattttgt gcagcgatgg
      721 gggcgggggg ggggggggcg cgcgccaggc ggggcggggc ggggcgaggg gcggggcggg
      781 gcgaggcgga gaggtgcggc ggcagccaat cagagcggcg cgctccgaaa gtttcctttt
      841 atggcgaggc ggcggcggcg gcggccctat aaaaagcgaa gcgcgcggcg ggcgggagtc
      901 gctgcgcgct gccttcgccc cgtgccccgc tccgccgccg cctcgcgccg cccgccccgg
      961 ctctgactga ccgcgttact cccacaggtg agcgggcggg acggcccttc tcctccgggc
     1021 tgtaattagc gcttggttta atgacggctt gtttcttttc tgtggctgcg tgaaagcctt
     1081 gaggggctcc gggagggccc tttgtgcggg gggagcggct cggggggtgc gtgcgtgtgt
     1141 gtgtgcgtgg ggagcgccgc gtgcggctcc gcgctgcccg gcggctgtga gcgctgcggg
     1201 cgcggcgcgg ggctttgtgc gctccgcagt gtgcgcgagg ggagcgcggc cgggggcggt
     1261 gccccgcggt gcgggggggg ctgcgagggg aacaaaggct gcgtgcgggg tgtgtgcgtg
     1321 ggggggtgag cagggggtgt gggcgcgtcg gtcgggctgc aaccccccct gcacccccct
     1381 ccccgagttg ctgagcacgg cccggcttcg ggtgcggggc tccgtacggg gcgtggcgcg
     1441 gggctcgccg tgccgggcgg ggggtggcgg caggtggggg tgccgggcgg ggcggggccg
     1501 cctcgggccg gggagggctc gggggagggg cgcggcggcc cccggagcgc cggcggctgt
     1561 cgaggcgcgg cgagccgcag ccattgcctt ttatggtaat cgtgcgagag ggcgcaggga
     1621 cttcctttgt cccaaatctg tgcggagccg aaatctggga ggcgccgccg caccccctct
     1681 agcgggcgcg gggcgaagcg gtgcggcgcc ggcaggaagg aaatgggcgg ggagggcctt
     1741 cgtgcgtcgc cgcgccgccg tccccttctc cctctccagc ctcggggctg tccgcggggg
     1801 gacggctgcc ttcggggggg acggggcagg gcggggttcg gcttctggcg tgtgaccggc
     1861 ggctctagac aattgtacta accttcttct ctttcctctc ctgacaggtt ggtgtacagt
     1921 agcttgctag caaagaattc atgagcctcc agctccgcag ctccgcccgc atccccagcg
     1981 gttccatctc gccattcatg cggatggcgc cgctggcatt tctgttgctg ttcacgctgc
     2041 cgcagcatct agctgaagct gccccttcct cagtcatagc cgcaacggag ctgcgttgtg
     2101 tttgcttaac cgtaactcca aaaattaatc ccaaattgat cgctaatttg gaggtgatcc
     2161 ctgcaggtcc acagtgccct acggtggaag tcatagctaa actgaaaaac cagaaggagg
     2221 tctgtctgga tccagaagct cctgtgataa agaaaatcat tcagaaaata ttgggcagtg
     2281 acaaaaagaa agctaagcgg aatgcactcg cagtggaaag aacggccagt gttcaatagg
     2341 cggccgcatt ctaccgggta ggggaggcgc ttttcccaag gcagtctgga gcatgcgctt
     2401 tagcagcccc gctgggcact tggcgctaca caagtggcct ctggcctcgc acacattcca
     2461 catccaccgg taggcgccaa ccggctccgt tctttggtgg ccccttcgcg ccaccttcta
     2521 ctcctcccct agtcaggaag ttcccccccg ccccgcagct cgcgtcgtgc aggacgtgac
     2581 aaatggaagt agcacgtctc actagtctcg tgcagatgga cagcaccgct gagcaatgga
     2641 agcgggtagg cctttggggc agcggccaat agcagctttg ctccttcgct ttctgggctc
     2701 agaggctggg aaggggtggg tccgggggcg ggctcagggg cgggctcagg ggcggggcgg
     2761 gcgcccgaag gtcctccgga ggcccggcat tctgcacgct tcaaaagcgc acgtctgccg
     2821 cgctgttctc ctcttcctca tctccgggac ccgggatgcc caagaagaag aggaaggtgt
     2881 ccaatttact gaccgtacac caaaatttgc ctgcattacc ggtcgatgca acgagtgatg
     2941 aggttcgcaa gaacctgatg gacatgttca gggatcgcca ggcgttttct gagcatacct
     3001 ggaaaatgct tctgtccgtt tgccggtcgt gggcggcatg gtgcaagttg aataaccgga
     3061 aatggtttcc cgcagaacct gaagatgttc gcgattatct tctatatctt caggcgcgcg
     3121 gtctggcagt aaaaactatc cagcaacatt tgggccagct aaacatgctt catcgtcggt
     3181 ccgggctgcc acgaccaagt gacagcaatg ctgtttcact ggttatgcgg cggatccgaa
     3241 aagaaaacgt tgatgccggt gaacgtgcaa aacaggctct agcgttcgaa cgcactgatt
     3301 tcgaccaggt tcgttcactc atggaaaata gcgatcgctg ccaggatata cgtaatctgg
     3361 catttctggg gattgcttat aacaccctgt tacgtatagc cgaaattgcc aggatcaggg
     3421 ttaaagatat ctcacgtact gacggtggga gaatgttaat ccatattggc agaacgaaaa
     3481 cgctggttag caccgcaggt gtagagaagg cacttagcct gggggtaact aaactggtcg
     3541 agcgatggat ttccgtctct ggtgtagctg atgatccgaa taactacctg ttttgccggg
     3601 tcagaaaaaa tggtgttgcc gcgccatctg ccaccagcca gctatcaact cgcgccctgg
     3661 aagggatttt tgaagcaact catcgattga tttacggcgc taaggatgac tctggtcaga
     3721 gatacctggc ctggtctgga cacagtgccc gtgtcggagc cgcgcgagat atggcccgcg
     3781 ctggagtttc aataccggag atcatgcaag ctggtggctg gaccaatgta aatattgtca
     3841 tgaactatat ccgtaacctg gatagtgaaa caggggcaat ggtgcgcctg ctggaagatg
     3901 gcgattagcc atgtttaagg gttccggttc cactaggtac aattcgatat caagcttatc
     3961 gataatcaac ctctggatta caaaatttgt gaaagattga ctggtattct taactatgtt
     4021 gctcctttta cgctatgtgg atacgctgct ttaatgcctt tgtatcatgc tattgcttcc
     4081 cgtatggctt tcattttctc ctccttgtat aaatcctggt tgctgtctct ttatgaggag
     4141 ttgtggcccg ttgtcaggca acgtggcgtg gtgtgcactg tgtttgctga cgcaaccccc
     4201 actggttggg gcattgccac cacctgtcag ctcctttccg ggactttcgc tttccccctc
     4261 cctattgcca cggcggaact catcgccgcc tgccttgccc gctgctggac aggggctcgg
     4321 ctgttgggca ctgacaattc cgtggtgttg tcggggaaat catcgtcctt tccttggctg
     4381 ctcgcctgtg ttgccacctg gattctgcgc gggacgtcct tctgctacgt cccttcggcc
     4441 ctcaatccag cggaccttcc ttcccgcggc ctgctgccgg ctctgcggcc tcttccgcgt
     4501 cttcgccttc gccctcagac gagtcggatc tccctttggg ccgcctcccc gcatcgatac
     4561 cgtcgacctc gatcgagacc tagaaaaaca tggagcaatc acaagtagca atacagcagc
     4621 taccaatgct gattgtgcct ggctagaagc acaagaggag gaggaggtgg gttttccagt
     4681 cacacctcag gtacctttaa gaccaatgac ttacaaggca gctgtagatc ttagccactt
     4741 tttaaaagaa aaggggggac tggaagggct aattcactcc caacgaagac aagatatcct
     4801 tgatctgtgg atctaccaca cacaaggcta cttccctgat tggcagaact acacaccagg
     4861 gccagggatc agatatccac tgacctttgg atggtgctac aagctagtac cagttgagca
     4921 agagaaggta gaagaagcca atgaaggaga gaacacccgc ttgttacacc ctgtgagcct
     4981 gcatgggatg gatgacccgg agagagaagt attagagtgg aggtttgaca gccgcctagc
     5041 atttcatcac atggcccgag agctgcatcc ggactgtact gggtctctct ggttagacca
     5101 gatctgagcc tgggagctct ctggctaact agggaaccca ctgcttaagc ctcaataaag
     5161 cttgccttga gtgcttcaag tagtgtgtgc ccgtctgttg tgtgactctg gtaactagag
     5221 atccctcaga cccttttagt cagtgtggaa aatctctagc agcatgtgag caaaaggcca
     5281 gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc
     5341 ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact
     5401 ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct
     5461 gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag
     5521 ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca
     5581 cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa
     5641 cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc
     5701 gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag
     5761 aagaacagta tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg
     5821 tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca
     5881 gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc
     5941 tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgagat tatcaaaaag
     6001 gatcttcacc tagatccttt taaattaaaa atgaagtttt aaatcaatct aaagtatata
     6061 tgagtaaact tggtctgaca gttaccaatg cttaatcagt gaggcaccta tctcagcgat
     6121 ctgtctattt cgttcatcca tagttgcctg actccccgtc gtgtagataa ctacgatacg
     6181 ggagggctta ccatctggcc ccagtgctgc aatgataccg cgagacccac gctcaccggc
     6241 tccagattta tcagcaataa accagccagc cggaagggcc gagcgcagaa gtggtcctgc
     6301 aactttatcc gcctccatcc agtctattaa ttgttgccgg gaagctagag taagtagttc
     6361 gccagttaat agtttgcgca acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc
     6421 gtcgtttggt atggcttcat tcagctccgg ttcccaacga tcaaggcgag ttacatgatc
     6481 ccccatgttg tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg tcagaagtaa
     6541 gttggccgca gtgttatcac tcatggttat ggcagcactg cataattctc ttactgtcat
     6601 gccatccgta agatgctttt ctgtgactgg tgagtactca accaagtcat tctgagaata
     6661 gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata cgggataata ccgcgccaca
     6721 tagcagaact ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa aactctcaag
     6781 gatcttaccg ctgttgagat ccagttcgat gtaacccact cgtgcaccca actgatcttc
     6841 agcatctttt actttcacca gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc
     6901 aaaaaaggga ataagggcga cacggaaatg ttgaatactc atactcttcc tttttcaata
     6961 ttattgaagc atttatcagg gttattgtct catgagcgga tacatatttg aatgtattta
     7021 gaaaaataaa caaatagggg ttccgcgcac atttccccga aaagtgccac ctgac
//
