LOCUS       sequence-212586-       10288 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Doxycycline-inducible overexpression of Sox2.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10288)
  AUTHORS   Mollaoglu G, Jones A, Wait SJ, Mukhopadhyay A, Jeong S, Arya R, 
            Camolotto SA, Mosbruger TL, Stubben CJ, Conley CJ, Bhutkar A, 
            Vahrenkamp JM, Berrett KC, Cessna MH, Lane TE, Witt BL, Salama ME, 
            Gertz J, Jones KB, Snyder EL, Oliver TG
  TITLE     The Lineage-Defining Transcription Factors SOX2 and NKX2-1 Determine
            Lung Cancer Cell Fate and Shape the Tumor Immune Microenvironment.
  JOURNAL   Immunity. 2018 Oct 16;49(4):764-779.e9. doi: 
            10.1016/j.immuni.2018.09.020.
  PUBMED    30332632
REFERENCE   2  (bases 1 to 10288)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.immuni.2018.09"; journalName: "Immunity"; date: 
            "2018-10-16- 16"; volume: "49"; issue: "4"; pages: "764-779.e9"
COMMENT     Sequence Label: Lenti-TetON-Sox2
FEATURES             Location/Qualifiers
     source          1..10288
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..19
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        47..273
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             274..454
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    501..626
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1119..1352
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1537..1581
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    1794..1911
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     protein_bind    1973..1991
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2009..2027
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2045..2063
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2081..2099
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     promoter        2111..2149
                     /label=minimal CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2146..2170
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     promoter        3167..4378
                     /label=UbC promoter
                     /note="human ubiquitin C promoter"
     primer_bind     4215..4234
                     /label=hUBCpro-F
                     /note="Human Ubiquitin C (UbC) promoter, forward primer"
     CDS             join(4437..5054,5055..5060,5061..5096,5097..5099,5100..5135,
                     5136..5138,5139..5174,5175..5183)
                     /codon_start=1
                     /product="improved tetracycline-controlled transactivator"
                     /label=rtTA-Advanced
                     /note="In the Tet-On(R) system, rtTA-Advanced binds to the 
                     Tet-responsive element and stimulates transcription only in
                     the presence of tetracycline or doxycycline."
                     /translation="MSRLDKSKVINGALELLNGVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALPIEMLDRHHTHFCPLEGESWQDFLRNNAKSYRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGGPTDALDDFDLDMLPA
                     DALDDFDLDMLPADALDDFDLDMLPG"
     misc_feature    join(5214..5788,5789..5791,5792..5800)
                     /label=IRES2
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(5381..5398)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     5608..5627
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             5801..6199
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     primer_bind     complement(6219..6239)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6637..6648)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             6853..7086
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    7158..7279
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7195..7214)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7249..7268
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      7319..7454
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(7474..7493)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(7475..7493)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(7503..7520)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(7503..7519)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7512..7534)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      7661..8116
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7748..7767)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7958..7979
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        8142..8246
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8247..8315,8316..9107)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8465..8484)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      9278..9866
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9767..9786
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     10020..10037
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    10154..10175
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(10190..10195,10196..10213,10214..10220)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    10228..10244
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     10233..10255
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     10252..10268
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     10252..10268
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     join(10287..10288,1..19)
                     /label=T3
                     /note="T3 promoter, forward primer"
ORIGIN
        1 aattaaccct cactaaaggg aacaaaagct ggagctgcaa gcttaatgta gtcttatgca
       61 atactcttgt agtcttgcaa catggtaacg atgagttagc aacatgcctt acaaggagag
      121 aaaaagcacc gtgcatgccg attggtggaa gtaaggtggt acgatcgtgc cttattagga
      181 aggcaacaga cgggtctgac atggattgga cgaaccactg aattgccgca ttgcagagat
      241 attgtattta agtgcctagc tcgatacata aacgggtctc tctggttaga ccagatctga
      301 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
      361 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
      421 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
      481 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
      541 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
      601 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
      661 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
      721 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
      781 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
      841 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
      901 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
      961 aagcggccgc tgatcttcag acctggagga ggagatatga gggacaattg gagaagtgaa
     1021 ttatataaat ataaagtagt aaaaattgaa ccattaggag tagcacccac caaggcaaag
     1081 agaagagtgg tgcagagaga aaaaagagca gtgggaatag gagctttgtt ccttgggttc
     1141 ttgggagcag caggaagcac tatgggcgca gcgtcaatga cgctgacggt acaggccaga
     1201 caattattgt ctggtatagt gcagcagcag aacaatttgc tgagggctat tgaggcgcaa
     1261 cagcatctgt tgcaactcac agtctggggc atcaagcagc tccaggcaag aatcctggct
     1321 gtggaaagat acctaaagga tcaacagctc ctggggattt ggggttgctc tggaaaactc
     1381 atttgcacca ctgctgtgcc ttggaatgct agttggagta ataaatctct ggaacagatt
     1441 tggaatcaca cgacctggat ggagtgggac agagaaatta acaattacac aagcttaata
     1501 cactccttaa ttgaagaatc gcaaaaccag caagaaaaga atgaacaaga attattggaa
     1561 ttagataaat gggcaagttt gtggaattgg tttaacataa caaattggct gtggtatata
     1621 aaattattca taatgatagt aggaggcttg gtaggtttaa gaatagtttt tgctgtactt
     1681 tctatagtga atagagttag gcagggatat tcaccattat cgtttcagac ccacctccca
     1741 accccgaggg gacaattctc gacctcgaga caaatggcag tattcatcca caattttaaa
     1801 agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat aatagcaaca
     1861 gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt tcgggtttat
     1921 tacagggaca gcagagatcc actttggccg cgaatcgata tgtcgagttt actccctatc
     1981 agtgatagag aacgtatgtc gagtttactc cctatcagtg atagagaacg tatgtcgagt
     2041 ttantcccta tcagtgatag agaacgtatg tcgagtttac tccctatcag tgatagagaa
     2101 cgtatgtcga ggtaggcgtg tacggtggga ggcctatata agcagagctc gtttagtgaa
     2161 ccgtcagatc gcctggaggt taacatgtat aacatgatgg agacggagct gaagccgccg
     2221 ggcccgcagc aagcttcggg gggcggcggc ggaggaggca acgccacggc ggcggcgacc
     2281 ggcggcaacc agaagaacag cccggaccgc gtcaagaggc ccatgaacgc cttcatggta
     2341 tggtcccggg ggcagcggcg taagatggcc caggagaacc ccaagatgca caactcggag
     2401 atcagcaagc gcctgggcgc ggagtggaaa cttttgtccg agaccgagaa gcggccgttc
     2461 atcgacgagg ccaagcggct gcgcgctctg cacatgaagg agcacccgga ttataaatac
     2521 cggccgcggc ggaaaaccaa gacgctcatg aagaaggata agtacacgct tcccggaggc
     2581 ttgctggccc ccggcgggaa cagcatggcg agcggggttg gggtgggcgc cggcctgggt
     2641 gcgggcgtga accagcgcat ggacagctac gcgcacatga acggctggag caacggcagc
     2701 tacagcatga tgcaggagca gctgggctac ccgcagcacc cgggcctcaa cgctcacggc
     2761 gcggcacaga tgcaaccgat gcaccgctac gacgtcagcg ccctgcagta caactccatg
     2821 accagctcgc agacctacat gaacggctcg cccacctaca gcatgtccta ctcgcagcag
     2881 ggcacccccg gtatggcgct gggctccatg ggctctgtgg tcaagtccga ggccagctcc
     2941 agcccccccg tggttacctc ttcctcccac tccagggcgc cctgccaggc cggggacctc
     3001 cgggacatga tcagcatgta cctccccggc gccgaggtgc cggagcccgc tgcgcccagt
     3061 agactgcaca tggcccagca ctaccagagc ggcccggtgc ccggcacggc cattaacggc
     3121 acactgcccc tgtcgcacat gtgattaatt aacccaccca agatctggcc tccgcgccgg
     3181 gttttggcgc ctcccgcggg cgcccccctc ctcacggcga gcgctgccac gtcagacgaa
     3241 gggcgcagcg agcgtcctga tccttccgcc cggacgctca ggacagcggc ccgctgctca
     3301 taagactcgg ccttagaacc ccagtatcag cagaaggaca ttttaggacg ggacttgggt
     3361 gactctaggg cactggtttt ctttccagag agcggaacag gcgaggaaaa gtagtccctt
     3421 ctcggcgatt ctgcggaggg atctccgtgg ggcggtgaac gccgatgatt atataaggac
     3481 gcgccgggtg tggcacagct agttccgtcg cagccgggat ttgggtcgcg gttcttgttt
     3541 gtggatcgct gtgatcgtca cttggtgagt agcgggctgc tgggctggcc ggggctttcg
     3601 tggccgccgg gccgctcggt gggacggaag cgtgtggaga gaccgccaag ggctgtagtc
     3661 tgggtccgcg agcaaggttg ccctgaactg ggggttgggg ggagcgcagc aaaatggcgg
     3721 ctgttcccga gtcttgaatg gaagacgctt gtgaggcggg ctgtgaggtc gttgaaacaa
     3781 ggtggggggc atggtgggcg gcaagaaccc aaggtcttga ggccttcgct aatgcgggaa
     3841 agctcttatt cgggtgagat gggctggggc accatctggg gaccctgacg tgaagtttgt
     3901 cactgactgg agaactcggt ttgtcgtctg ttgcgggggc ggcagttatg gcggtgccgt
     3961 tgggcagtgc acccgtacct ttgggagcgc gcgccctcgt cgtgtcgtga cgtcacccgt
     4021 tctgttggct tataatgcag ggtggggcca cctgccggta ggtgtgcggt aggcttttct
     4081 ccgtcgcagg acgcagggtt cgggcctagg gtaggctctc ctgaatcgac aggcgccgga
     4141 cctctggtga ggggagggat aagtgaggcg tcagtttctt tggtcggttt tatgtaccta
     4201 tcttcttaag tagctgaagc tccggttttg aactatgcgc tcggggttgg cgagtgtgtt
     4261 ttgtgaagtt ttttaggcac cttttgaaat gtaatcattt gggtcaatat gtaattttca
     4321 gtgttagact agtaaattgt ccgctaaatt ctggccgttt ttggcttttt tgttagacga
     4381 agcttggtac cgagctcgga tctccacccc gtaccggtcc tgcagtcgaa ttcaccatgt
     4441 ctagactgga caagagcaaa gtcataaacg gagctctgga attactcaat ggtgtcggta
     4501 tcgaaggcct gacgacaagg aaactcgctc aaaagctggg agttgagcag cctaccctgt
     4561 actggcacgt gaagaacaag cgggccctgc tcgatgccct gccaatcgag atgctggaca
     4621 ggcatcatac ccacttctgc cccctggaag gcgagtcatg gcaagacttt ctgcggaaca
     4681 acgccaagtc ataccgctgt gctctcctct cacatcgcga cggggctaaa gtgcatctcg
     4741 gcacccgccc aacagagaaa cagtacgaaa ccctggaaaa tcagctcgcg ttcctgtgtc
     4801 agcaaggctt ctccctggag aacgcactgt acgctctgtc cgccgtgggc cactttacac
     4861 tgggctgcgt attggaggaa caggagcatc aagtagcaaa agaggaaaga gagacaccta
     4921 ccaccgattc tatgccccca cttctgagac aagcaattga gctgttcgac cggcagggag
     4981 ccgaacctgc cttccttttc ggcctggaac taatcatatg tggcctggag aaacagctaa
     5041 agtgcgaaag cggcgggccg accgacgccc ttgacgattt tgacttagac atgctcccag
     5101 ccgatgccct tgacgacttt gaccttgata tgctgcctgc tgacgctctt gacgattttg
     5161 accttgacat gctccccggg taactaagta aggatccgcg gccgcaaatt ccgcccctct
     5221 ccctcccccc cccctaacgt tactggccga agccgcttgg aataaggccg gtgtgcgttt
     5281 gtctatatgt tattttccac catattgccg tcttttggca atgtgagggc ccggaaacct
     5341 ggccctgtct tcttgacgag cattcctagg ggtctttccc ctctcgccaa aggaatgcaa
     5401 ggtctgttga atgtcgtgaa ggaagcagtt cctctggaag cttcttgaag acaaacaacg
     5461 tctgtagcga ccctttgcag gcagcggaac cccccacctg gcgacaggtg cctctgcggc
     5521 caaaagccac gtgtataaga tacacctgca aaggcggcac aaccccagtg ccacgttgtg
     5581 agttggatag ttgtggaaag agtcaaatgg ctctcctcaa gcgtattcaa caaggggctg
     5641 aaggatgccc agaaggtacc ccattgtatg ggatctgatc tggggcctcg gtgcacatgc
     5701 tttacatgtg tttagtcgag gttaaaaaaa cgtctaggcc ccccgaacca cggggacgtg
     5761 gttttccttt gaaaaacacg atgataagct tgccacaacc atggccaagc ctttgtctca
     5821 agaagaatcc accctcattg aaagagcaac ggctacaatc aacagcatcc ccatctctga
     5881 agactacagc gtcgccagcg cagctctctc tagcgacggc cgcatcttca ctggtgtcaa
     5941 tgtatatcat tttactgggg gaccttgtgc agaactcgtg gtgctgggca ctgctgctgc
     6001 tgcggcagct ggcaacctga cttgtatcgt cgcgatcgga aatgagaaca ggggcatctt
     6061 gagcccctgc ggacggtgcc gacaggtgct tctcgatctg catcctggga tcaaagccat
     6121 agtgaaggac agtgatggac agccgacggc agttgggatt cgtgaattgc tgccctctgg
     6181 ttatgtgtgg gagggctaag atatcgtatt cttaactatg ttgctccttt tacgctatgt
     6241 ggatacgctg ctttaatgcc tttgtatcat gctattgctt cccgtatggc tttcattttc
     6301 tcctccttgt ataaatcctg gttgctgtct ctttatgagg agttgtggcc cgttgtcagg
     6361 caacgtggcg tggtgtgcac tgtgtttgct gacgcaaccc ccactggttg gggcattgcc
     6421 accacctgtc agctcctttc cgggactttc gctttccccc tccctattgc cacggcggaa
     6481 ctcatcgccg cctgccttgc ccgctgctgg acaggggctc ggctgttggg cactgacaat
     6541 tccgtggtgt tgtcggggaa gctgacgtcc tttccatggc tgctcgcctg tgttgccacc
     6601 tggattctgc gcgggacgtc cttctgctac gtcccttcgg ccctcaatcc agcggacctt
     6661 ccttcccgcg gcctgctgcc ggctctgcgg cctcttccgc gtcttcgcct tcgccctcag
     6721 acgagtcgga tctccctttg ggccgcctcc ccgcctgttt cgcctcggcg tccggtctag
     6781 aggtaccttt aagaccaatg acttacaagg cagctgtaga tcttagccac tttttaaaag
     6841 aaaagggggg actggaaggg ctaattcact cccaacgaag acaagatctg ctttttgctt
     6901 gtactgggtc tctctggtta gaccagatct gagcctggga gctctctggc taactaggga
     6961 acccactgct taagcctcaa taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc
     7021 tgttgtgtga ctctggtaac tagagatccc tcagaccctt ttagtcagtg tggaaaatct
     7081 ctagcagtag tagttcatgt catcttatta ttcagtattt ataacttgca aagaaatgaa
     7141 tatcagagag tgagaggaac ttgtttattg cagcttataa tggttacaaa taaagcaata
     7201 gcatcacaaa tttcacaaat aaagcatttt tttcactgca ttctagttgt ggtttgtcca
     7261 aactcatcaa tgtatcttat catgtctggc tctagctatc ccgcccctaa ctccgcccat
     7321 cccgccccta actccgccca gttccgccca ttctccgccc catggctgac taattttttt
     7381 tatttatgca gaggccgagg ccgcctcggc ctctgagcta ttccagaagt agtgaggagg
     7441 cttttttgga ggcctaggga cgtacccaat tcgccctata gtgagtcgta ttacgcgcgc
     7501 tcactggccg tcgttttaca acgtcgtgac tgggaaaacc ctggcgttac ccaacttaat
     7561 cgccttgcag cacatccccc tttcgccagc tggcgtaata gcgaagaggc ccgcaccgat
     7621 cgcccttccc aacagttgcg cagcctgaat ggcgaatggg acgcgccctg tagcggcgca
     7681 ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg ctacacttgc cagcgcccta
     7741 gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca cgttcgccgg ctttccccgt
     7801 caagctctaa atcgggggct ccctttaggg ttccgattta gtgctttacg gcacctcgac
     7861 cccaaaaaac ttgattaggg tgatggttca cgtagtgggc catcgccctg atagacggtt
     7921 tttcgccctt tgacgttgga gtccacgttc tttaatagtg gactcttgtt ccaaactgga
     7981 acaacactca accctatctc ggtctattct tttgatttat aagggatttt gccgatttcg
     8041 gcctattggt taaaaaatga gctgatttaa caaaaattta acgcgaattt taacaaaata
     8101 ttaacgctta caatttaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt
     8161 tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc
     8221 ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc
     8281 ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa
     8341 aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg
     8401 gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag
     8461 ttctgctatg tggcgcggta ttatcccgta ttgacgccgg gcaagagcaa ctcggtcgcc
     8521 gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta
     8581 cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg
     8641 cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca
     8701 acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac
     8761 caaacgacga gcgtgacacc acgatgcctg tagcaatggc aacaacgttg cgcaaactat
     8821 taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg
     8881 ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata
     8941 aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta
     9001 agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa
     9061 atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag
     9121 tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg
     9181 tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact
     9241 gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg
     9301 taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc
     9361 aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata
     9421 ctgttcttct agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta
     9481 catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc
     9541 ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg
     9601 ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac
     9661 agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg
     9721 taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt
     9781 atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct
     9841 cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg
     9901 ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata
     9961 accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca
    10021 gcgagtcagt gagcgaggaa gcggaagagc gcccaatacg caaaccgcct ctccccgcgc
    10081 gttggccgat tcattaatgc agctggcacg acaggtttcc cgactggaaa gcgggcagtg
    10141 agcgcaacgc aattaatgtg agttagctca ctcattaggc accccaggct ttacacttta
    10201 tgcttccggc tcgtatgttg tgtggaattg tgagcggata acaatttcac acaggaaaca
    10261 gctatgacca tgattacgcc aagcgcgc
//
