LOCUS       sequence-212591-        1629 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses FLAG-NES-RNMT 121-476 D203A (""Delta-N-RNMT"") in 
            mammalian cells. Overexpression inhibits cytoplasmic cap 
            methylation..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1629)
  AUTHORS   Trotman JB, Giltmier AJ, Mukherjee C, Schoenberg DR
  TITLE     RNA guanine-7 methyltransferase catalyzes the methylation of 
            cytoplasmically recapped RNAs.
  JOURNAL   Nucleic Acids Res. 2017 Oct 13;45(18):10726-10739. doi: 
            10.1093/nar/gkx801.
  PUBMED    28981715
REFERENCE   2  (bases 1 to 1629)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Nucleic Acids Res"; date: "2017-10-13- 13"; volume: "45"; issue: 
            "18"; pages: "10726-10739"
COMMENT     Sequence Label: pcDNA3-Delta-N-RNMT
FEATURES             Location/Qualifiers
     source          1..1629
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        156..359
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     310..330
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     404..423
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        404..422
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             443..466
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             479..505
                     /codon_start=1
                     /product="nuclear export signal from the HIV Rev protein 
                     (Fischer et al., 1995)"
                     /label=NES
                     /translation="LPPLERLTL"
     CDS             515..526
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
ORIGIN
        1 acttggcagt acatcaagtg tatcatatgc caagtacgcc ccctattgac gtcaatgacg
       61 gtaaatggcc cgcctggcat tatgcccagt acatgacctt atgggacttt cctacttggc
      121 agtacatcta cgtattagtc atcgctatta ccatggtgat gcggttttgg cagtacatca
      181 atgggcgtgg atagcggttt gactcacggg gatttccaag tctccacccc attgacgtca
      241 atgggagttt gttttggcac caaaatcaac gggactttcc aaaatgtcgt aacaactccg
      301 ccccattgac gcaaatgggc ggtaggcgtg tacggtggga ggtctatata agcagagctc
      361 tctggctaac tagagaaccc actgcttact ggcttatcga aattaatacg actcactata
      421 gggagaccca agcttgatgg gcgactacaa agacgatgac gacaagagca gccttcagct
      481 accaccgctt gagagactta ctcttgatgg ccatatcgaa ggtcgtcatg atggcactca
      541 aaataagaga aaaatagcac ttgaggatgt tcctgaaaag cagaaaaatc tggaagaagg
      601 acacagctca acagtggctg cccattacaa tgaacttcag gaagttggtt tggagaagcg
      661 tagtcaaagt cgtatttttt acctaagaaa ctttaataat tggatgaaaa gtgttctcat
      721 tggagaattt ttggaaaagg tacgacagaa gaaaaaacgt gatatcactg ttttggccct
      781 gggatgtggt aaaggtggag atttgctgaa atggaaaaaa ggaagaatta acaagctagt
      841 ttgtactgat attgccgatg tttctgtcaa acagtgtcag cagcggtatg aggacatgaa
      901 aaatcgtcgt gatagtgaat atattttcag tgcagaattt ataactgctg acagctcaaa
      961 ggaacttctg attgacaaat ttcgtgaccc acaaatgtgt tttgacatct gcagttgtca
     1021 gtttgtctgt cattactcat ttgagtctta tgagcaggct gacatgatgc tgagaaatgc
     1081 gtgtgagaga cttagccctg ggggctattt tattggtact actcccaata gctttgaatt
     1141 gataagacgc cttgaagctt cagaaacaga atcatttgga aatgaaatat atactgtgaa
     1201 atttcagaag aaaggagatt atcctttatt tggctgcaaa tatgacttca acttggaagg
     1261 tgttgtggat gttcctgaat tcttggtcta ttttccattg ctaaatgaaa tggcaaagaa
     1321 gtacaatatg aaactagtct acaaaaaaac atttctggaa ttctacgaag aaaagattaa
     1381 gaacaatgaa aataaaatgc tcttaaaacg aatgcaggcc ttggagccat atcctgcaaa
     1441 tgagagttct aaacttgtct ctgagaaggt ggatgactat gaacatgcag caaagtacat
     1501 gaagaacagt caagtaaggt tacctttggg aaccttaagt aaatcagaat gggaagctac
     1561 aagtatttac ttggtgtttg cctttgagaa acagcagtga tgacgagctc ggatccacta
     1621 gtaacggcc
//
