LOCUS       sequence-212592-        2134 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses N-terminally GST-tagged human RNMT in bacterial cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2134)
  AUTHORS   Trotman JB, Giltmier AJ, Mukherjee C, Schoenberg DR
  TITLE     RNA guanine-7 methyltransferase catalyzes the methylation of 
            cytoplasmically recapped RNAs.
  JOURNAL   Nucleic Acids Res. 2017 Oct 13;45(18):10726-10739. doi: 
            10.1093/nar/gkx801.
  PUBMED    28981715
REFERENCE   2  (bases 1 to 2134)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Nucleic Acids Res"; date: "2017-10-13- 13"; volume: "45"; issue: 
            "18"; pages: "10726-10739"
COMMENT     Sequence Label: pGEX-2T-GST-RNMT
FEATURES             Location/Qualifiers
     source          1..2134
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             1..654
                     /codon_start=1
                     /product="glutathione S-transferase from Schistosoma 
                     japonicum"
                     /label=GST
                     /translation="MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKK
                     FELGLEFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVLDIRY
                     GVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTHPDFMLYDALDVVL
                     YMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIAWPLQGWQATFGGGDHPPK"
     primer_bind     612..634
                     /label=pGEX 5'
                     /note="pGEX vectors, Glutathione-S-transferase, forward 
                     primer"
     CDS             661..678
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
ORIGIN
        1 atgtccccta tactaggtta ttggaaaatt aagggccttg tgcaacccac tcgacttctt
       61 ttggaatatc ttgaagaaaa atatgaagag catttgtatg agcgcgatga aggtgataaa
      121 tggcgaaaca aaaagtttga attgggtttg gagtttccca atcttcctta ttatattgat
      181 ggtgatgtta aattaacaca gtctatggcc atcatacgtt atatagctga caagcacaac
      241 atgttgggtg gttgtccaaa agagcgtgca gagatttcaa tgcttgaagg agcggttttg
      301 gatattagat acggtgtttc gagaattgca tatagtaaag actttgaaac tctcaaagtt
      361 gattttctta gcaagctacc tgaaatgctg aaaatgttcg aagatcgttt atgtcataaa
      421 acatatttaa atggtgatca tgtaacccat cctgacttca tgttgtatga cgctcttgat
      481 gttgttttat acatggaccc aatgtgcctg gatgcgttcc caaaattagt ttgttttaaa
      541 aaacgtattg aagctatccc acaaattgat aagtacttga aatccagcaa gtatatagca
      601 tggcctttgc agggctggca agccacgttt ggtggtggcg accatcctcc aaaatcggat
      661 ctggttccgc gtggatcccc gggaattcat gcaaattctg caaaagcaga agaatatgaa
      721 aagatgtctc ttgaacaggc aaaagcgtca gtgaattctg aaacagagtc ttcattcaat
      781 attaatgaaa acacaacagc ttctgggact gggctttctg aaaagacttc tgtctgtagg
      841 caagtagaca tagcaagaaa gagaaaagag tttgaagatg atcttgtaaa ggaaagttct
      901 agttgtggga aagacactcc atccaagaag agaaaacttg atcctgaaat tgtcccagag
      961 gaaaaagatt gtggtgatgc tgaaggcaat tcaaagaaaa gaaaaagaga aactgaggat
     1021 gttccaaaag ataaatcttc tactggagat ggcactcaaa ataagagaaa aatagcactt
     1081 gaggatgttc ctgaaaagca gaaaaatctg gaagaaggac acagctcaac agtggctgcc
     1141 cattacaatg aacttcagga agttggtttg gagaagcgta gtcaaagtcg tattttttac
     1201 ctaagaaact ttaataattg gatgaaaagt gttctcattg gagaattttt ggaaaaggta
     1261 cgacagaaga aaaaacgtga tatcactgtt ttggacctgg gatgtggtaa aggtggagat
     1321 ttgctgaaat ggaaaaaagg aagaattaac aagctagttt gtactgatat tgccgatgtt
     1381 tctgtcaaac agtgtcagca gcggtatgag gacatgaaaa atcgtcgtga tagtgaatat
     1441 attttcagtg cagaatttat aactgctgac agctcaaagg aacttctgat tgacaaattt
     1501 cgtgacccac aaatgtgttt tgacatctgc agttgtcagt ttgtctgtca ttactcattt
     1561 gagtcttatg agcaggctga catgatgctg agaaatgcgt gtgagagact tagccctggg
     1621 ggctatttta ttggtactac tcccaatagc tttgaattga taagacgcct tgaagcttca
     1681 gaaacagaat catttggaaa tgaaatatat actgtgaaat ttcagaagaa aggagattat
     1741 cctttatttg gctgcaaata tgacttcaac ttggaaggtg ttgtggatgt tcctgaattc
     1801 ttggtctatt ttccattgct aaatgaaatg gcaaagaagt acaatatgaa actagtctac
     1861 aaaaaaacat ttctggaatt ctacgaagaa aagattaaga acaatgaaaa taaaatgctc
     1921 ttaaaacgaa tgcaggcctt ggagccatat cctgcaaatg agagttctaa acttgtctct
     1981 gagaaggtgg atgactatga acatgcagca aagtacatga agaacagtca agtaaggtta
     2041 cctttgggaa ccttaagtaa atcagaatgg gaagctacaa gtatttactt ggtgtttgcc
     2101 tttgagaaac agcagtgaaa ttcatcgtga ctga
//
