LOCUS       sequence-212597-        8384 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Luciferase lentivirus based enhancer assay vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8384)
  TITLE     pLS-SV40-mP-Rluc
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8384)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pLS-SV40-mP-Rluc
FEATURES             Location/Qualifiers
     source          1..8384
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(47..66)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        238..617
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        619..817
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     772..792
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             835..1015
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    1062..1187
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1684..1917
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2102..2146
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2444..2561
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     enhancer        3057..3248
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(3080..3100)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        3332..3363
                     /label=minP
                     /note="minimal TATA-box promoter with low basal activity"
     regulatory      3403..3412
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             3409..4344
                     /codon_start=1
                     /product="Renilla luciferase"
                     /label=hRluc
                     /note="human codon-optimized"
                     /translation="MASKVYDPEQRKRMITGPQWWARCKQMNVLDSFINYYDSEKHAEN
                     AVIFLHGNAASSYLWRHVVPHIEPVARCIIPDLIGMGKSGKSGNGSYRLLDHYKYLTAW
                     FELLNLPKKIIFVGHDWGACLAFHYSYEHQDKIKAIVHAESVVDVIESWDEWPDIEEDI
                     ALIKSEEGEKMVLENNFFVETMLPSKIMRKLEPEEFAAYLEPFKEKGEVRRPTLSWPRE
                     IPLVKGGKPDVVQIVRNYNAYLRASDDLPKMFIESDPGFFSNAIVEGAKKFPNTEFVKV
                     KGLHFSQEDAPDEMGKYIKSFVERVLKNEQ"
     protein_bind    complement(4384..4417)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     misc_feature    4473..5061
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(4526..4546)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4944..4955)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(5064..5080)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5065..5081)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             6390..6570
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     rep_origin      complement(6632..7220)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6712..6731)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7391..8182,8183..8251))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8014..8033
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8252..8356)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gtcgacggat cgggagatct cccgatcccc tatggtgcac tctcagtaca atctgctctg
       61 atgccgcata gttaagccag tatctgctcc ctgcttgtgt gttggaggtc gctgagtagt
      121 gcgcgagcaa aatttaagct acaacaaggc aaggcttgac cgacaattgc atgaagaatc
      181 tgcttagggt taggcgtttt gcgctgcttc gcgatgtacg ggccagatat acgcgttgac
      241 attgattatt gactagttat taatagtaat caattacggg gtcattagtt catagcccat
      301 atatggagtt ccgcgttaca taacttacgg taaatggccc gcctggctga ccgcccaacg
      361 acccccgccc attgacgtca ataatgacgt atgttcccat agtaacgcca atagggactt
      421 tccattgacg tcaatgggtg gagtatttac ggtaaactgc ccacttggca gtacatcaag
      481 tgtatcatat gccaagtacg ccccctattg acgtcaatga cggtaaatgg cccgcctggc
      541 attatgccca gtacatgacc ttatgggact ttcctacttg gcagtacatc tacgtattag
      601 tcatcgctat taccatggtg atgcggtttt ggcagtacat caatgggcgt ggatagcggt
      661 ttgactcacg gggatttcca agtctccacc ccattgacgt caatgggagt ttgttttggc
      721 accaaaatca acgggacttt ccaaaatgtc gtaacaactc cgccccattg acgcaaatgg
      781 gcggtaggcg tgtacggtgg gaggtctata taagcagcgc gttttgcctg tactgggtct
      841 ctctggttag accagatctg agcctgggag ctctctggct aactagggaa cccactgctt
      901 aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac
      961 tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtggc
     1021 gcccgaacag ggacttgaaa gcgaaaggga aaccagagga gctctctcga cgcaggactc
     1081 ggcttgctga agcgcgcacg gcaagaggcg aggggcggcg actggtgagt acgccaaaaa
     1141 ttttgactag cggaggctag aaggagagag atgggtgcga gagcgtcagt attaagcggg
     1201 ggagaattag atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag aaaaaatata
     1261 aattaaaaca tatagtatgg gcaagcaggg agctagaacg attcgcagtt aatcctggcc
     1321 tgttagaaac atcagaaggc tgtagacaaa tactgggaca gctacaacca tcccttcaga
     1381 caggatcaga agaacttaga tcattatata atacagtagc aaccctctat tgtgtgcatc
     1441 aaaggataga gataaaagac accaaggaag ctttagacaa gatagaggaa gagcaaaaca
     1501 aaagtaagac caccgcacag caagcggccg gccgctgatc ttcagacctg gaggaggaga
     1561 tatgagggac aattggagaa gtgaattata taaatataaa gtagtaaaaa ttgaaccatt
     1621 aggagtagca cccaccaagg caaagagaag agtggtgcag agagaaaaaa gagcagtggg
     1681 aataggagct ttgttccttg ggttcttggg agcagcagga agcactatgg gcgcagcgtc
     1741 aatgacgctg acggtacagg ccagacaatt attgtctggt atagtgcagc agcagaacaa
     1801 tttgctgagg gctattgagg cgcaacagca tctgttgcaa ctcacagtct ggggcatcaa
     1861 gcagctccag gcaagaatcc tggctgtgga aagataccta aaggatcaac agctcctggg
     1921 gatttggggt tgctctggaa aactcatttg caccactgct gtgccttgga atgctagttg
     1981 gagtaataaa tctctggaac agatttggaa tcacacgacc tggatggagt gggacagaga
     2041 aattaacaat tacacaagct taatacactc cttaattgaa gaatcgcaaa accagcaaga
     2101 aaagaatgaa caagaattat tggaattaga taaatgggca agtttgtgga attggtttaa
     2161 cataacaaat tggctgtggt atataaaatt attcataatg atagtaggag gcttggtagg
     2221 tttaagaata gtttttgctg tactttctat agtgaataga gttaggcagg gatattcacc
     2281 attatcgttt cagacccacc tcccaacccc gaggggaccc gacaggcccg aaggaataga
     2341 agaagaaggt ggagagagag acagagacag atccattcga ttagtgaacg gatcggcact
     2401 gcgtgcgcca attctgcaga caaatggcag tattcatcca caattttaaa agaaaagggg
     2461 ggattggggg gtacagtgca ggggaaagaa tagtagacat aatagcaaca gacatacaaa
     2521 ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt tcgggtttat tacagggaca
     2581 gcagagatcc agtttggtta gtaccgggcc cggtgctttg ctctgagcca gcccaccagt
     2641 ttggaatgac tcctttttat gacttgaatt ttcaagtata aagtctagtg ctaaatttaa
     2701 tttgaacaac tgtatagttt ttgctggttg ggggaaggaa aaaaaatggt ggcagtgttt
     2761 ttttcagaat tagaagtgaa atgaaaactt gttgtgtgtg aggatttcta atgacatgtg
     2821 gtggttgcat actgagtgaa gccggtgagc attctgccat gtcaccccct cgtgctcagt
     2881 aatgtacttt acagaaatcc taaactcaaa agattgatat aaaccatgct tcttgtgtat
     2941 atccggtctc ttctctgggt agtctcactc agcctgcatt tctgccaggg cccgctctag
     3001 agcgcagcac catggcctga aataacctct gaaagaggaa cttggttagg taccttctga
     3061 ggcggaaaga accagctgtg gaatgtgtgt cagttagggt gtggaaagtc cccaggctcc
     3121 ccagcaggca gaagtatgca aagcatgcat ctcaattagt cagcaaccag gtgtggaaag
     3181 tccccaggct ccccagcagg cagaagtatg caaagcatgc atctcaatta gtcagcaacc
     3241 atagtcccgc ccctaactcc gcccatcccg cccctaactc cgcccagttc cgcccattct
     3301 ccgccccatg gctgtctaga cctgcaggca ctagagggta tataatggaa gctcgacttc
     3361 cagcttggca atccggtact gtaccggtgt actgttggta aagccaccat ggcttccaag
     3421 gtgtacgacc ccgagcaacg caaacgcatg atcactgggc ctcagtggtg ggctcgctgc
     3481 aagcaaatga acgtgctgga ctccttcatc aactactatg attccgagaa gcacgccgag
     3541 aacgccgtga tttttctgca tggtaacgct gcctccagct acctgtggag gcacgtcgtg
     3601 cctcacatcg agcccgtggc tagatgcatc atccctgatc tgatcggaat gggtaagtcc
     3661 ggcaagagcg ggaatggctc atatcgcctc ctggatcact acaagtacct caccgcttgg
     3721 ttcgagctgc tgaaccttcc aaagaaaatc atctttgtgg gccacgactg gggggcttgt
     3781 ctggcctttc actactccta cgagcaccaa gacaagatca aggccatcgt ccatgctgag
     3841 agtgtcgtgg acgtgatcga gtcctgggac gagtggcctg acatcgagga ggatatcgcc
     3901 ctgatcaaga gcgaagaggg cgagaaaatg gtgcttgaga ataacttctt cgtcgagacc
     3961 atgctcccaa gcaagatcat gcggaaactg gagcctgagg agttcgctgc ctacctggag
     4021 ccattcaagg agaagggcga ggttagacgg cctaccctct cctggcctcg cgagatccct
     4081 ctcgttaagg gaggcaagcc cgacgtcgtc cagattgtcc gcaactacaa cgcctacctt
     4141 cgggccagcg acgatctgcc taagatgttc atcgagtccg accctgggtt cttttccaac
     4201 gctattgtcg agggagctaa gaagttccct aacaccgagt tcgtgaaggt gaagggcctc
     4261 cacttcagcc aggaggacgc tccagatgaa atgggtaagt acatcaagag cttcgtggag
     4321 cgcgtgctga agaacgagca gtaattctag agtcggggcg gccggaattc gtcgagggac
     4381 ctaataactt cgtatagcat acattatacg aagttataca tgtttaaggg ttccggttcc
     4441 actaggtaca attcgatatc aagcttatcg ataatcaacc tctggattac aaaatttgtg
     4501 aaagattgac tggtattctt aactatgttg ctccttttac gctatgtgga tacgctgctt
     4561 taatgccttt gtatcatgct attgcttccc gtatggcttt cattttctcc tccttgtata
     4621 aatcctggtt gctgtctctt tatgaggagt tgtggcccgt tgtcaggcaa cgtggcgtgg
     4681 tgtgcactgt gtttgctgac gcaaccccca ctggttgggg cattgccacc acctgtcagc
     4741 tcctttccgg gactttcgct ttccccctcc ctattgccac ggcggaactc atcgccgcct
     4801 gccttgcccg ctgctggaca ggggctcggc tgttgggcac tgacaattcc gtggtgttgt
     4861 cggggaaatc atcgtccttt ccttggctgc tcgcctgtgt tgccacctgg attctgcgcg
     4921 ggacgtcctt ctgctacgtc ccttcggccc tcaatccagc ggaccttcct tcccgcggcc
     4981 tgctgccggc tctgcggcct cttccgcgtc ttcgccttcg ccctcagacg agtcggatct
     5041 ccctttgggc cgcctccccg catcgatacc gtcgacctcg atcgagacct agaaaaacat
     5101 ggagcaatca caagtagcaa tacagcagct accaatgctg attgtgcctg gctagaagca
     5161 caagaggagg aggaggtggg ttttccagtc acacctcagg taccaagcat ggggtaaagt
     5221 actgttctca tcacatcata tcaaggttat ataccatcaa tattgccaca gatgttactt
     5281 agccttttaa tatttctcta atttagtgta tatgcaatga tagttctctg atttctgaga
     5341 ttgagtttct catgtgtaat gattatttag agtttctctt tcatctgttc aaatttttgt
     5401 ctagttttat tttttactga tttgtaagac ttctttttat aatctgcata ttacaattct
     5461 ctttactggg gtgttgcaaa tattttctgt cattctatgg cctgactttt cttaatggtt
     5521 ttttaatttt aaaaataagt cttaatattc atgcaatcta attaacaatc ttttctttgt
     5581 ggttaggact ttgagtcata agaaattttt ctctacactg aagtcatgat ggcatgcttc
     5641 tatattattt tctaaaagat ttaaagtttt gccttctcca tttagactta taattcactg
     5701 gaattttttt gtgtgtatgg tatgacatat gggttccctt ttatttttta catataaata
     5761 tatttccctg tttttctaaa aaagaaaaag atcatcattt tcccattgta aaatgccata
     5821 tttttttcat aggtcactta catatatcaa tgggtctgtt tctgagctct actctatttt
     5881 atcagcctca ctgtctatcc ccacacatct catgctttgc tctaaatctt gatatttagt
     5941 ggaacattct ttcccatttt gttctacaag aatatttttg ttattgtctt ttgggcttct
     6001 atatacattt tagaatgagg ttggcaagtt ctgtagatct tagccacttt ttaaaagaaa
     6061 aggggggact ggaagggcta attcactccc aacgaagaca agatatcctt gatctgtgga
     6121 tctaccacac acaaggctac ttccctgatt ggcagaacta cacaccaggg ccagggatca
     6181 gatatccact gacctttgga tggtgctaca agctagtacc agttgagcaa gagaaggtag
     6241 aagaagccaa tgaaggagag aacacccgct tgttacaccc tgtgagcctg catgggatgg
     6301 atgacccgga gagagaagta ttagagtgga ggtttgacag ccgcctagca tttcatcaca
     6361 tggcccgaga gctgcatccg gactgtactg ggtctctctg gttagaccag atctgagcct
     6421 gggagctctc tggctaacta gggaacccac tgcttaagcc tcaataaagc ttgccttgag
     6481 tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg taactagaga tccctcagac
     6541 ccttttagtc agtgtggaaa atctctagca gcatgtgagc aaaaggccag caaaaggcca
     6601 ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc
     6661 atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc
     6721 aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg
     6781 gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta
     6841 ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg
     6901 ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac
     6961 acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag
     7021 gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga agaacagtat
     7081 ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat
     7141 ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag cagattacgc
     7201 gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct gacgctcagt
     7261 ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt atcaaaaagg atcttcacct
     7321 agatcctttt aaattaaaaa tgaagtttta aatcaatcta aagtatatat gagtaaactt
     7381 ggtctgacag ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc tgtctatttc
     7441 gttcatccat agttgcctga ctccccgtcg tgtagataac tacgatacgg gagggcttac
     7501 catctggccc cagtgctgca atgataccgc gagacccacg ctcaccggct ccagatttat
     7561 cagcaataaa ccagccagcc ggaagggccg agcgcagaag tggtcctgca actttatccg
     7621 cctccatcca gtctattaat tgttgccggg aagctagagt aagtagttcg ccagttaata
     7681 gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt gtcacgctcg tcgtttggta
     7741 tggcttcatt cagctccggt tcccaacgat caaggcgagt tacatgatcc cccatgttgt
     7801 gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag
     7861 tgttatcact catggttatg gcagcactgc ataattctct tactgtcatg ccatccgtaa
     7921 gatgcttttc tgtgactggt gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc
     7981 gaccgagttg ctcttgcccg gcgtcaatac gggataatac cgcgccacat agcagaactt
     8041 taaaagtgct catcattgga aaacgttctt cggggcgaaa actctcaagg atcttaccgc
     8101 tgttgagatc cagttcgatg taacccactc gtgcacccaa ctgatcttca gcatctttta
     8161 ctttcaccag cgtttctggg tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa
     8221 taagggcgac acggaaatgt tgaatactca tactcttcct ttttcaatat tattgaagca
     8281 tttatcaggg ttattgtctc atgagcggat acatatttga atgtatttag aaaaataaac
     8341 aaataggggt tccgcgcaca tttccccgaa aagtgccacc tgac
//
