LOCUS       sequence-212598-        8686 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian expression plasmid for soluble BG505 SOSIP.664.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 8686)
  AUTHORS   Heredia JD, Park J, Choi H, Gill KS, Procko E
  TITLE     Conformational engineering of HIV-1 Env based on mutational 
            tolerance in the CD4 and PG16 bound states.
  JOURNAL   J Virol. 2019 Mar 20. pii: JVI.00219-19. doi: 10.1128/JVI.00219-19.
  PUBMED    30894475
REFERENCE   2  (bases 1 to 8686)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Virol. 
            2019 Mar 20. pii: JVI.00219-19. doi: 10.1128/JVI.00219-19."
COMMENT     Sequence Label: pCEP4-BG505.SOSIP-8his
FEATURES             Location/Qualifiers
     source          1..8686
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        86..231
                     /label=HSV TK promoter
                     /note="herpes simplex virus thymidine kinase promoter"
     CDS             274..1311
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPDRE
                     MGEAN"
     primer_bind     complement(1309..1328)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    1353..1401
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     enhancer        2055..2434
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2435..2638
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2589..2609
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     2635..2659
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             4691..4714
                     /codon_start=1
                     /product="8xHis affinity tag"
                     /label=8xHis
                     /translation="HHHHHHHH"
     polyA_signal    4740..4874
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4764..4783)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     4818..4837
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(5017..5039)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     rep_origin      5599..7388
                     /label=oriP
                     /note="Epstein-Barr virus oriP replication origin (Yates et
                     al., 2000)"
ORIGIN
        1 gccctgcttc atccccgtgg cccgttgctc gcgtttgctg gcggtgtccc cggaagaaat
       61 atatttgcat gtctttagtt ctatgatgac acaaaccccg cccagcgtct tgtcattggc
      121 gaattcgaac acgcagatgc agtcggggcg gcgcggtccg aggtccactt cgcatattaa
      181 ggtgacgcgt gtggcctcga acaccgagcg accctgcagc gacccgctta acagcgtcaa
      241 cagcgtgccg cagatcccgg ggggcaatga gatatgaaaa agcctgaact caccgcgacg
      301 tctgtcgaga agtttctgat cgaaaagttc gacagcgtct ccgacctgat gcagctctcg
      361 gagggcgaag aatctcgtgc tttcagcttc gatgtaggag ggcgtggata tgtcctgcgg
      421 gtaaatagct gcgccgatgg tttctacaaa gatcgttatg tttatcggca ctttgcatcg
      481 gccgcgctcc cgattccgga agtgcttgac attggggaat tcagcgagag cctgacctat
      541 tgcatctccc gccgtgcaca gggtgtcacg ttgcaagacc tgcctgaaac cgaactgccc
      601 gctgttctgc agccggtcgc ggaggccatg gatgcgatcg ctgcggccga tcttagccag
      661 acgagcgggt tcggcccatt cggaccgcaa ggaatcggtc aatacactac atggcgtgat
      721 ttcatatgcg cgattgctga tccccatgtg tatcactggc aaactgtgat ggacgacacc
      781 gtcagtgcgt ccgtcgcgca ggctctcgat gagctgatgc tttgggccga ggactgcccc
      841 gaagtccggc acctcgtgca cgcggatttc ggctccaaca atgtcctgac ggacaatggc
      901 cgcataacag cggtcattga ctggagcgag gcgatgttcg gggattccca atacgaggtc
      961 gccaacatct tcttctggag gccgtggttg gcttgtatgg agcagcagac gcgctacttc
     1021 gagcggaggc atccggagct tgcaggatcg ccgcggctcc gggcgtatat gctccgcatt
     1081 ggtcttgacc aactctatca gagcttggtt gacggcaatt tcgatgatgc agcttgggcg
     1141 cagggtcgat gcgacgcaat cgtccgatcc ggagccggga ctgtcgggcg tacacaaatc
     1201 gcccgcagaa gcgcggccgt ctggaccgat ggctgtgtag aagtactcgc cgatagtgga
     1261 aaccgacgcc ccagcactcg tccggatcgg gagatggggg aggctaactg aaacacggaa
     1321 ggagacaata ccggaaggaa cccgcgctat gacggcaata aaaagacaga ataaaacgca
     1381 cgggtgttgg gtcgtttgtt cataaacgcg gggttcggtc ccagggctgg cactctgtcg
     1441 ataccccacc gagaccccat tggggccaat acgcccgcgt ttcttccttt tccccacccc
     1501 accccccaag ttcgggtgaa ggcccagggc tcgcagccaa cgtcggggcg gcaggccctg
     1561 ccatagccac tggccccgtg ggttagggac ggggtccccc atggggaatg gtttatggtt
     1621 cgtgggggtt attattttgg gcgttgcgtg gggtcaggtc cacgactgga ctgagcagac
     1681 agacccatgg tttttggatg gcctgggcat ggaccgcatg tactggcgcg acacgaacac
     1741 cgggcgtctg tggctgccaa acacccccga cccccaaaaa ccaccgcgcg gatttctggc
     1801 gtgccaagct agtcgaccaa ttctcatgtt tgacagctta tcatcgcaga tccgggcaac
     1861 gttgttgcca ttgctgcagg cgcagaactg gtaggtatgg aagatctata cattgaatca
     1921 atattggcaa ttagccatat tagtcattgg ttatatagca taaatcaata ttggctattg
     1981 gccattgcat acgttgtatc tatatcataa tatgtacatt tatattggct catgtccaat
     2041 atgaccgcca tgttgacatt gattattgac tagttattaa tagtaatcaa ttacggggtc
     2101 attagttcat agcccatata tggagttccg cgttacataa cttacggtaa atggcccgcc
     2161 tggctgaccg cccaacgacc cccgcccatt gacgtcaata atgacgtatg ttcccatagt
     2221 aacgccaata gggactttcc attgacgtca atgggtggag tatttacggt aaactgccca
     2281 cttggcagta catcaagtgt atcatatgcc aagtccgccc cctattgacg tcaatgacgg
     2341 taaatggccc gcctggcatt atgcccagta catgacctta cgggactttc ctacttggca
     2401 gtacatctac gtattagtca tcgctattac catggtgatg cggttttggc agtacaccaa
     2461 tgggcgtgga tagcggtttg actcacgggg atttccaagt ctccacccca ttgacgtcaa
     2521 tgggagtttg ttttggcacc aaaatcaacg ggactttcca aaatgtcgta ataaccccgc
     2581 cccgttgacg caaatgggcg gtaggcgtgt acggtgggag gtctatataa gcagagctcg
     2641 tttagtgaac cgtcagatct ctagaagctg ggtaccagct gctagcgcca ccatgccaat
     2701 gggctccctt cagcccttgg ctaccctgta ccttcttggg atgttggtcg catctgtgct
     2761 tgccgcagaa aacttgtggg taactgtcta ctatggagta cccgtttgga aagacgcaga
     2821 aacaacgctt ttttgcgcat ccgacgcgaa ggcatacgaa acggaaaagc acaatgtttg
     2881 ggcaacgcat gcctgcgtcc cgacagaccc aaatcctcag gagattcacc ttgagaatgt
     2941 aacagaggaa ttcaacatgt ggaagaataa catggtagaa caaatgcata cggatattat
     3001 cagtctttgg gaccagagcc ttaaaccttg cgttaaactt acgcccctct gcgtaactct
     3061 gcaatgtact aacgtaacga ataacattac ggacgacatg cgcggcgaac tgaagaattg
     3121 cagctttaat atgacaacgg agttgcgaga taaaaagcag aaggtgtaca gcctcttcta
     3181 caggcttgac gttgtgcaaa taaacgaaaa ccaggggaac aggagcaata actccaataa
     3241 agaatacaga ttgatcaact gtaacactag cgcaataacc caggcctgcc cgaaggtgtc
     3301 atttgagccg attcctatac actattgcgc cccggcaggg tttgcgatct tgaaatgcaa
     3361 ggataagaag tttaatggaa ctggtccctg tcccagtgtg agtacggttc agtgtaccca
     3421 cggcataaag ccagtggtat ccactcagct tctcctcaat ggcagcctcg cagaggaaga
     3481 ggtcatgata cggagtgaga atattactaa caatgccaag aatatcttgg tccaatttaa
     3541 tacccccgtc cagattaatt gtacccgacc taataataat accagaaaat ccattcgcat
     3601 cggaccaggt caggcgttct atgccacggg agatataata ggtgacatcc gacaggcgca
     3661 ttgcaatgta agcaaggcca catggaatga gactctgggt aaagttgtca agcagctcag
     3721 gaagcatttc gggaacaata ccatcatccg atttgcgaac agtagtgggg gtgatcttga
     3781 agtaactaca cattcattta actgcggcgg ggagtttttt tattgtaaca catccgggtt
     3841 gttcaactcc acttggatat ctaacactag tgttcaggga tcaaactcca caggatcaaa
     3901 cgatagtata acacttcctt gtcgcatcaa acagattatc aacatgtggc agcgaatcgg
     3961 ccaggcgatg tacgccccgc ctatccaggg agtaatacgg tgcgtaagca acataacggg
     4021 ccttatactt acgcgcgatg gaggcagcac taattctacc actgagacgt tccggcctgg
     4081 cggtggggac atgcgagaca actggcggtc tgagttgtac aagtacaaag tggttaaaat
     4141 cgaacccctg ggtgtcgccc caacgagatg taaacgacgg gtagtgggaa ggaggagaag
     4201 gcgacgggcg gtcggcattg gggccgtttt cttgggtttt ctgggggcgg ctgggagcac
     4261 tatgggagca gctagtatga ccctgaccgt acaggcgagg aacctcctgt ccggtatagt
     4321 gcaacaacag tcaaaccttc ttagggctcc tgaggcgcag caacatctcc ttaagttgac
     4381 tgtctggggc ataaagcaac tccaagcgcg ggttctcgcg gtggaaagat accttaggga
     4441 ccaacaactt ttggggattt ggggttgttc aggcaaactc atctgttgca ctaacgttcc
     4501 ttggaactcc tcatggtcta accgaaatct ctccgagatc tgggataata tgacatggtt
     4561 gcagtgggat aaggagatta gcaattacac tcaaataatc tatgggctcc tggaggaaag
     4621 ccagaatcag caggagaaga atgaacagga tcttctggcc cttgatgggt caggctctgg
     4681 aggatctgga caccatcatc atcaccatca ccattagtga ctcgaggccg gcaaggccgg
     4741 atccagacat gataagatac attgatgagt ttggacaaac cacaactaga atgcagtgaa
     4801 aaaaatgctt tatttgtgaa atttgtgatg ctattgcttt atttgtaacc attataagct
     4861 gcaataaaca agttaacaac aacaattgca ttcattttat gtttcaggtt cagggggagg
     4921 tgtgggaggt tttttaaagc aagtaaaacc tctacaaatg tggtatggct gattatgatc
     4981 cggctgcctc gcgcgtttcg gtgatgacgg tgaaaacctc tgacacatgc agctcccgga
     5041 gacggtcaca gcttgtctgt aagcggatgc cgggagcaga caagcccgtc agggcgcgtc
     5101 agcgggtgtt ggcgggtgtc ggggcgcagc catgaggtcg actctagagg atcgatcccc
     5161 gccccggacg aactaaacct gactacgaca tctctgcccc ttcttcgcgg ggcagtgcat
     5221 gtaatccctt cagttggttg gtacaacttg ccaactgggc cctgttccac atgtgacacg
     5281 gggggggacc aaacacaaag gggttctctg actgtagttg acatccttat aaatggatgt
     5341 gcacatttgc caacactgag tggctttcat cctggagcag actttgcagt ctgtggactg
     5401 caacacaaca ttgcctttat gtgtaactct tggctgaagc tcttacacca atgctggggg
     5461 acatgtacct cccaggggcc caggaagact acgggaggct acaccaacgt caatcagagg
     5521 ggcctgtgta gctaccgata agcggaccct caagagggca ttagcaatag tgtttataag
     5581 gcccccttgt taaccctaaa cgggtagcat atgcttcccg ggtagtagta tatactatcc
     5641 agactaaccc taattcaata gcatatgtta cccaacggga agcatatgct atcgaattag
     5701 ggttagtaaa agggtcctaa ggaacagcga tatctcccac cccatgagct gtcacggttt
     5761 tatttacatg gggtcaggat tccacgaggg tagtgaacca ttttagtcac aagggcagtg
     5821 gctgaagatc aaggagcggg cagtgaactc tcctgaatct tcgcctgctt cttcattctc
     5881 cttcgtttag ctaatagaat aactgctgag ttgtgaacag taaggtgtat gtgaggtgct
     5941 cgaaaacaag gtttcaggtg acgcccccag aataaaattt ggacgggggg ttcagtggtg
     6001 gcattgtgct atgacaccaa tataaccctc acaaacccct tgggcaataa atactagtgt
     6061 aggaatgaaa cattctgaat atctttaaca atagaaatcc atggggtggg gacaagccgt
     6121 aaagactgga tgtccatctc acacgaattt atggctatgg gcaacacata atcctagtgc
     6181 aatatgatac tggggttatt aagatgtgtc ccaggcaggg accaagacag gtgaaccatg
     6241 ttgttacact ctatttgtaa caaggggaaa gagagtggac gccgacagca gcggactcca
     6301 ctggttgtct ctaacacccc cgaaaattaa acggggctcc acgccaatgg ggcccataaa
     6361 caaagacaag tggccactct tttttttgaa attgtggagt gggggcacgc gtcagccccc
     6421 acacgccgcc ctgcggtttt ggactgtaaa ataagggtgt aataacttgg ctgattgtaa
     6481 ccccgctaac cactgcggtc aaaccacttg cccacaaaac cactaatggc accccgggga
     6541 atacctgcat aagtaggtgg gcgggccaag ataggggcgc gattgctgcg atctggagga
     6601 caaattacac acacttgcgc ctgagcgcca agcacagggt tgttggtcct catattcacg
     6661 aggtcgctga gagcacggtg ggctaatgtt gccatgggta gcatatacta cccaaatatc
     6721 tggatagcat atgctatcct aatctatatc tgggtagcat aggctatcct aatctatatc
     6781 tgggtagcat atgctatcct aatctatatc tgggtagtat atgctatcct aatttatatc
     6841 tgggtagcat aggctatcct aatctatatc tgggtagcat atgctatcct aatctatatc
     6901 tgggtagtat atgctatcct aatctgtatc cgggtagcat atgctatcct aatagagatt
     6961 agggtagtat atgctatcct aatttatatc tgggtagcat atactaccca aatatctgga
     7021 tagcatatgc tatcctaatc tatatctggg tagcatatgc tatcctaatc tatatctggg
     7081 tagcataggc tatcctaatc tatatctggg tagcatatgc tatcctaatc tatatctggg
     7141 tagtatatgc tatcctaatt tatatctggg tagcataggc tatcctaatc tatatctggg
     7201 tagcatatgc tatcctaatc tatatctggg tagtatatgc tatcctaatc tgtatccggg
     7261 tagcatatgc tatcctcatg catatacagt cagcatatga tacccagtag tagagtggga
     7321 gtgctatcct ttgcatatgc cgccacctcc caagggggcg tgaattttcg ctgcttgtcc
     7381 ttttcctgct ggttgctccc attcttaggt gaatttaagg aggccaggct aaagccgtcg
     7441 catgtctgat tgctcaccag gtaaatgtcg ctaatgtttt ccaacgcgag aaggtgttga
     7501 gcgcggagct gagtgacgtg acaacatggg tatgcccaat tgccccatgt tgggaggacg
     7561 aaaatggtga caagacagat ggccagaaat acaccaacag cacgcatgat gtctactggg
     7621 gatttattct ttagtgcggg ggaatacacg gcttttaata cgattgaggg cgtctcctaa
     7681 caagttacat cactcctgcc cttcctcacc ctcatctcca tcacctcctt catctccgtc
     7741 atctccgtca tcaccctccg cggcagcccc ttccaccata ggtggaaacc agggaggcaa
     7801 atctactcca tcgtcaaagc tgcacacagt caccctgata ttgcaggtag gagcgggctt
     7861 tgtcataaca aggtccttaa tcgcatcctt caaaacctca gcaaatatat gagtttgtaa
     7921 aaagaccatg aaataacaga caatggactc ccttagcggg ccaggttgtg ggccgggtcc
     7981 aggggccatt ccaaagggga gacgactcaa tggtgtaaga cgacattgtg gaatagcaag
     8041 ggcagttcct cgccttaggt tgtaaaggga ggtcttacta cctccatata cgaacacacc
     8101 ggcgacccaa gttccttcgt cggtagtcct ttctacgtga ctcctagcca ggagagctct
     8161 taaaccttct gcaatgttct caaatttcgg gttggaacct ccttgaccac gatgctttcc
     8221 aaaccaccct ccttttttgc gcctgcctcc atcaccctga ccccggggtc cagtgcttgg
     8281 gccttctcct gggtcatctg cggggccctg ctctatcgct cccgggggca cgtcaggctc
     8341 accatctggg ccaccttctt ggtggtattc aaaataatcg gcttccccta cagggtggaa
     8401 aaatggcctt ctacctggag ggggcctgcg cggtggagac ccggatgatg atgactgact
     8461 actgggactc ctgggcctct tttctccacg tccacgacct ctccccctgg ctctttcacg
     8521 acttcccccc ctggctcttt cacgtcctct accccggcgg cctccactac ctcctcgacc
     8581 ccggcctcca ctacctcctc gaccccggcc tccactgcct cctcgacccc ggcctccacc
     8641 tcctgctcct gcccctcctg ctcctgcccc tcctcctgct cctgcc
//
