LOCUS       sequence-21260-e         737 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 737)
  AUTHORS   Fiordalisi JJ, Johnson RL 2nd, Ulku AS, Der CJ, Cox AD
  TITLE     Mammalian expression vectors for Ras family proteins: generation and
            use of expression constructs to analyze Ras family function.
  JOURNAL   Methods Enzymol. 2001;332:3-36. doi: 10.1016/s0076-6879(01)32189-4.
  PUBMED    11305105
REFERENCE   2  (bases 1 to 737)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Methods 
            Enzymol."; date: "2001"; volume: "332:3-36. doi"; pages: " 
            10.1016/s0076-6879(01)32189-4"
COMMENT     Sequence Label: pCGN R-Ras wt
FEATURES             Location/Qualifiers
     source          1..737
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     5'UTR           97..136
                     /label=HSV TK 5'-UTR
                     /note="5' untranslated region from the herpes simplex virus
                     thymidine kinase gene"
     CDS             166..192
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             172..198
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /note="This is a variant of the standard HA tag, which has 
                     sequence YPYDVPDYA. Some anti-HA antibodies may not 
                     recognize this variant."
                     /translation="YDVPDYASL"
ORIGIN
        1 gcagagctgt ttagtgaacc gtcagatcgc ctggagacgc catccacgct gttttgacct
       61 ccatagaaga caccgggacc gatccagcct ccgggggatc ttggtggcgt gaaactcccg
      121 cacctcttcg gccagcgcct tgtagaagcg cgtatggctt ctagctatcc ttatgacgtg
      181 cctgactatg ccagcctggg aggaccttct agcggatcca tgagctctgg tgctgcttct
      241 ggtacaggtc gtggtcgtcc acgtggtgga ggtccaggtc ccggggaccc cccgcccagc
      301 gagacacaca agctggtggt cgtgggcggc ggcggcgtgg gcaagagcgc gctgaccatc
      361 cagttcatcc agtcctactt cgtgtctgac tacgacccca ctattgagga ctcctacacg
      421 aagatctgca gtgtggatgg catcccagcc cggctggaca tcctggacac cgcgggccag
      481 gaagagttcg gggccatgag agagcagtac atgcgtgctg gccacggctt cctgctggtg
      541 ttcgccatta atgaccggca gagtttcaac gaggtgggca agctcttcac gcagattctg
      601 cgggtcaagg accgcgacga cttccccgtt gtgttggtcg ggaacaaggc agatctggag
      661 tcacagcgcc aggtcccccg atcagaagcc tctgccttcg gcgcctccca ccacgtggcc
      721 tactttgagg cctcggc
//
