LOCUS       sequence-21261-e         781 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 781)
  AUTHORS   Fiordalisi JJ, Johnson RL 2nd, Ulku AS, Der CJ, Cox AD
  TITLE     Mammalian expression vectors for Ras family proteins: generation and
            use of expression constructs to analyze Ras family function.
  JOURNAL   Methods Enzymol. 2001;332:3-36. doi: 10.1016/s0076-6879(01)32189-4.
  PUBMED    11305105
REFERENCE   2  (bases 1 to 781)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Methods 
            Enzymol."; date: "2001"; volume: "332:3-36. doi"; pages: " 
            10.1016/s0076-6879(01)32189-4"
COMMENT     Sequence Label: pCGN R-Ras 38V
FEATURES             Location/Qualifiers
     source          1..781
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     5'UTR           100..139
                     /label=HSV TK 5'-UTR
                     /note="5' untranslated region from the herpes simplex virus
                     thymidine kinase gene"
     CDS             169..195
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             175..201
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /note="This is a variant of the standard HA tag, which has 
                     sequence YPYDVPDYA. Some anti-HA antibodies may not 
                     recognize this variant."
                     /translation="YDVPDYASL"
ORIGIN
        1 ttagcagagc tcgttagtga accgtcagat cgcctggaga cgccatccac gctgttttga
       61 cctccataga agacaccggg accgatccag cctccggggg atcttggtgg cgtgaaactc
      121 ccgcacctct tcggccagcg ccttgtagaa gcgcgtatgg cttctagcta tccttatgac
      181 gtgcctgact atgccagcct gggaggacct tctagcggat cccatatgag ctctggtgct
      241 gcttccggta caggtcgtgg tcgtccacgt ggtggaggtc caggtcccgg ggaccctccg
      301 cctagcgaga cacacaagct tgtggtcgtg ggtggtgtcg gcgtgggcaa gagcgcgctg
      361 accatccagt tcatccagtc ctacttcgtg tctgactacg accccactat tgaggactcc
      421 tacacgaaga tctgcagtgt ggatggcatc ccagcccggc tggacatcct ggacaccgcg
      481 ggccaggaag agttcggggc catgagagag cagtacatgc gtgctggcca cggcttcctg
      541 ctggtgttcg ccattaatga ccggcagagt ttcaacgagg tgggcaagct cttcacgcag
      601 attctgcggg tcaaggaccg cgacgacttc cccgttgtgt tggtcgggaa caaggcagat
      661 ctggagtcac agcgccaggt cccccgatca gaagcctctg ccttcggcgc ctcccaccac
      721 gtggcctact ttgangcctc ggcaaactgc gtctcancgt ggacgagctt tgancanctg
      781 t
//
