LOCUS       sequence-212626-       11952 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  For membrane localized optoDroplets.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11952)
  AUTHORS   Dine E, Gil AA, Uribe G, Brangwynne CP, Toettcher JE
  TITLE     Protein Phase Separation Provides Long-Term Memory of Transient 
            Spatial Stimuli.
  JOURNAL   Cell Syst. 2018 May 24. pii: S2405-4712(18)30190-X. doi: 
            10.1016/j.cels.2018.05.002.
  PUBMED    29859829
REFERENCE   2  (bases 1 to 11952)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Syst. 
            2018 May 24. pii: S2405-4712(18)30190-X. doi: 
            10.1016/j.cels.2018.05.002."
COMMENT     Sequence Label: Myristoylated optoDroplet
FEATURES             Location/Qualifiers
     source          1..11952
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             1..634
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    681..806
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1303..1536
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1721..1765
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2050..2167
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2320..2727
                     /label=SFFV promoter
                     /note="spleen focus-forming virus long terminal repeat 
                     (LTR) promoter"
     CDS             2761..2781
                     /codon_start=1
                     /product="N-myristoylation signal from Src kinase (Pellman 
                     et al., 1985; Kaplan et al., 1988)"
                     /label=myr
                     /translation="MGSSKSK"
     CDS             3186..3197
                     /codon_start=1
                     /product="WELQut protease recognition and cleavage site"
                     /label=WELQut site
                     /translation="WELQ"
     CDS             3457..4152
                     /codon_start=1
                     /product="monomeric red fluorescent protein optimized for 
                     use as a fusion tag (Shemiakina et al., 2012)"
                     /label=FusionRed
                     /note="mammalian codon-optimized"
                     /translation="MVSELIKENMPMKLYMEGTVNNHHFKCTSEGEGKPYEGTQTMRIK
                     VVEGGPLPFAFDILATSFMYGSRTFIKHPPGIPDFFKQSFPEGFTWERVTTYEDGGVLT
                     ATQDTSLQDGCLIYNVKVRGVNFPANGPVMQKKTLGWEASTETMYPADGGLEGACDMAL
                     KLVGGGHLICNLETTYRSKKPATNLKMPGVYNVDHRLERIKEADDETYVEQHEVAVARY
                     STGGAGDGGK"
     CDS             4171..5664
                     /codon_start=1
                     /product="N-terminal photolyase homology region of 
                     Arabidopsis thaliana cryptochrome 2"
                     /label=CRY2PHR
                     /note="binds to CIB1 upon exposure to blue light (Kennedy 
                     et al., 2010)"
                     /translation="MKMDKKTIVWFRRDLRIEDNPALAAAAHEGSVFPVFIWCPEEEGQ
                     FYPGRASRWWMKQSLAHLSQSLKALGSDLTLIKTHNTISAILDCIRVTGATKVVFNHLY
                     DPVSLVRDHTVKEKLVERGISVQSYNGDLLYEPWEIYCEKGKPFTSFNSYWKKCLDMSI
                     ESVMLPPPWRLMPITAAAEAIWACSIEELGLENEAEKPSNALLTRAWSPGWSNADKLLN
                     EFIEKQLIDYAKNSKKVVGNSTSLLSPYLHFGEISVRHVFQCARMKQIIWARDKNSEGE
                     ESADLFLRGIGLREYSRYICFNFPFTHEQSLLSHLRFFPWDADVDKFKAWRQGRTGYPL
                     VDAGMRELWATGWMHNRIRVIVSSFAVKFLLLPWKWGMKYFWDTLLDADLECDILGWQY
                     ISGSIPDGHELDRLDNPALQGAKYDPEGEYIRQWLPELARLPTEWIHHPWDAPLTVLKA
                     SGVELGTNYAKPIVDIDTARELLAKAISRTREAQIMIGAA"
     misc_feature    5816..6404
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(5869..5889)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6287..6298)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(6407..6423)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6408..6424)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             6514..6747
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     promoter        complement(6772..6790)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(6773..6790)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(7080..7099)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     complement(7313..7332)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     7432..7454
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(7492..7510)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        7578..7682
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7683..7751,7752..8543)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7901..7920)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8714..9302
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9203..9222
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     9456..9473
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(9543..9563)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        9548..9877
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      9728..9863
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     intron          11011..11076
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             11206..11226
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    11651..11785
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(11688..11707)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     11742..11761
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
ORIGIN
        1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
       61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
      121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
      181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
      241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
      301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
      361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
      421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
      481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
      541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
      601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
      661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
      721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
      781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
      841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
      901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
      961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
     1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
     1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
     1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
     1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
     1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
     1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
     1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
     1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
     1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
     1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
     1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
     1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
     1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
     1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
     1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
     1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
     1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccaaa tggcagtatt
     2041 catccacaat tttaaaagaa aaggggggat tggggggtac agtgcagggg aaagaatagt
     2101 agacataata gcaacagaca tacaaactaa agaattacaa aaacaaatta caaaaattca
     2161 aaattttcgg gtttattaca gggacagcag agatccagtt tggatcgata agcttgatat
     2221 cgaattcctg cagccccgat aaaataaaag attttattta gtctccagaa aaagggggga
     2281 atgaaagacc ccacctgtag gtttggcaag ctagctgcag taacgccatt ttgcaaggca
     2341 tggaaaaata ccaaaccaag aatagagaag ttcagatcaa gggcgggtac atgaaaatag
     2401 ctaacgttgg gccaaacagg atatctgcgg tgagcagttt cggccccggc ccggggccaa
     2461 gaacagatgg tcaccgcagt ttcggccccg gcccgaggcc aagaacagat ggtccccaga
     2521 tatggcccaa ccctcagcag tttcttaaga cccatcagat gtttccaggc tcccccaagg
     2581 acctgaaatg accctgcgcc ttatttgaat taaccaatca gcctgcttct cgcttctgtt
     2641 cgcgcgcttc tgcttcccga gctctataaa agagctcaca acccctcact cggcgcgcca
     2701 gtcctccgac agactgagtc gcccgggggg gatctggagc tctcgagaat tctcacgcgt
     2761 atgggttcct ccaaatccaa gcccaaggac gcatctcagc gacgtcgcgc ctcaaacgat
     2821 tatacccaac aagcaaccca aagctatggg gcctacccca cccagcccgg gcagggctat
     2881 tcccagcaga gcagtcagcc ctacggacag cagagttaca gtggttatag ccagtccacg
     2941 gacacttcag gctatggcca gagcagctat tcttcttatg gccagagcca gaacacaggc
     3001 tatggaactc agtcaactcc ccagggatat ggctcgactg gcggctatgg cagtagccag
     3061 agctcccaat cgtcttacgg gcagcagtcc tcctatcctg gctatggcca gcagccagct
     3121 cccagcagca cctcgggaag ttacggtagc agttctcaga gcagcagcta tgggcagccc
     3181 cagagtggga gctacagcca gcagcctagc tatggtggac agcagcaaag ctatggacag
     3241 cagcaaagct ataatccccc tcagggctat ggacagcaga accagtacaa cagcagcagt
     3301 ggtggtggag gtggaggtgg aggtggaggt aactatggcc aagatcaatc ctccatgagt
     3361 agtggtggtg gcagtggtgg cggttatggc aatcaagacc agagtggtgg aggtggcagc
     3421 ggtggctatg gacagcagga ccgtggaggt ggaggaatgg tgagcgagct gattaaggag
     3481 aacatgccca tgaagctgta catggagggc accgtgaaca accaccactt caagtgcaca
     3541 tccgagggcg aaggcaagcc ctacgagggc acccagacca tgagaatcaa ggtcgtcgag
     3601 ggcggccctc tccccttcgc cttcgacatc ctggctacca gcttcatgta cggcagcaga
     3661 accttcatca agcaccctcc gggcatcccc gacttcttta agcagtcctt ccctgagggc
     3721 ttcacatggg agagagtcac cacatacgaa gacgggggcg tgctgaccgc tacccaggac
     3781 accagcctcc aggacggctg cctcatctac aacgtcaagg ttagaggggt gaacttccca
     3841 gccaacggcc ctgtgatgca gaagaaaaca ctcggctggg aggcctccac cgagacgatg
     3901 taccccgctg acggcggcct ggaaggcgca tgtgacatgg ccctgaagct cgtgggcggg
     3961 ggccacctga tctgcaacct tgagaccaca tacagatcca agaaacccgc tacgaacctc
     4021 aagatgcccg gcgtctacaa cgtggaccac agactggaaa gaatcaagga ggccgacgat
     4081 gagacctacg tcgagcagca cgaggtggct gtggccagat actctactgg tggcgctggt
     4141 gatggaggta aagcggccac gcgtacgcgt atgaagatgg acaaaaagac tatagtttgg
     4201 tttagaagag acctaaggat tgaggataat cctgcattag cagcagctgc tcacgaagga
     4261 tctgtttttc ctgtcttcat ttggtgtcct gaagaagaag gacagtttta tcctggaaga
     4321 gcttcaagat ggtggatgaa acaatcactt gctcacttat ctcaatcctt gaaggctctt
     4381 ggatctgacc tcactttaat caaaacccac aacacgattt cagcgatctt ggattgtatc
     4441 cgcgttaccg gtgctacaaa agtcgtcttt aaccacctct atgatcctgt ttcgttagtt
     4501 cgggaccata ccgtaaagga gaagctggtg gaacgtggga tctctgtgca aagctacaat
     4561 ggagatctat tgtatgaacc gtgggagata tactgcgaaa agggcaaacc ttttacgagt
     4621 ttcaattctt actggaagaa atgcttagat atgtcgattg aatccgttat gcttcctcct
     4681 ccttggcggt tgatgccaat aactgcagcg gctgaagcga tttgggcgtg ttcgattgaa
     4741 gaactagggc tggagaatga ggccgagaaa ccgagcaatg cgttgttaac tagagcttgg
     4801 tctccaggat ggagcaatgc tgataagtta ctaaatgagt tcatcgagaa gcagttgata
     4861 gattatgcaa agaacagcaa gaaagttgtt gggaattcta cttcactact ttctccgtat
     4921 ctccatttcg gggaaataag cgtcagacac gttttccagt gtgcccggat gaaacaaatt
     4981 atatgggcaa gagataagaa cagtgaagga gaagaaagtg cagatctttt tcttagggga
     5041 atcggtttaa gagagtattc tcggtatata tgtttcaact tcccgtttac tcacgagcaa
     5101 tcgttgttga gtcatcttcg gtttttccct tgggatgctg atgttgataa gttcaaggcc
     5161 tggagacaag gcaggaccgg ttatccgttg gtggatgccg gaatgagaga gctttgggct
     5221 accggatgga tgcataacag aataagagtg attgtttcaa gctttgctgt gaagtttctt
     5281 ctccttccat ggaaatgggg aatgaagtat ttctgggata cacttttgga tgctgatttg
     5341 gaatgtgaca tccttggctg gcagtatatc tctgggagta tccccgatgg ccacgagctt
     5401 gatcgcttgg acaatcccgc gttacaaggc gccaaatatg acccagaagg tgagtacata
     5461 aggcaatggc ttcccgagct tgcgagattg ccaactgaat ggatccatca tccatgggac
     5521 gctcctttaa ccgtactcaa agcttctggt gtggaactcg gaacaaacta tgcgaaaccc
     5581 attgtagaca tcgacacagc tcgtgagcta ctagctaaag ctatttcaag aacccgtgaa
     5641 gcacagatca tgatcggagc agcagcccgg gatccaccgg tcgccaccgg ttcagggagc
     5701 ggatccggct cagggagtgc ggccgcaact cccacctgca acatgcgtga ctgactgagg
     5761 ccgcgactct agagtcgacc tgcaggcatg caagcttgat atcaagctta tcgataatca
     5821 acctctggat tacaaaattt gtgaaagatt gactggtatt cttaactatg ttgctccttt
     5881 tacgctatgt ggatacgctg ctttaatgcc tttgtatcat gctattgctt cccgtatggc
     5941 tttcattttc tcctccttgt ataaatcctg gttgctgtct ctttatgagg agttgtggcc
     6001 cgttgtcagg caacgtggcg tggtgtgcac tgtgtttgct gacgcaaccc ccactggttg
     6061 gggcattgcc accacctgtc agctcctttc cgggactttc gctttccccc tccctattgc
     6121 cacggcggaa ctcatcgccg cctgccttgc ccgctgctgg acaggggctc ggctgttggg
     6181 cactgacaat tccgtggtgt tgtcggggaa atcatcgtcc tttccttggc tgctcgcctg
     6241 tgttgccacc tggattctgc gcgggacgtc cttctgctac gtcccttcgg ccctcaatcc
     6301 agcggacctt ccttcccgcg gcctgctgcc ggctctgcgg cctcttccgc gtcttcgcct
     6361 tcgccctcag acgagtcgga tctccctttg ggccgcctcc ccgcatcgat accgtcgacc
     6421 tcgagggaat taattcgagc tcggtacctt taagaccaat gacttacaag gcagctgtag
     6481 atcttagcca ctttttaaaa gaaaaggggg gactggaagg gctaattcac tcccaacgaa
     6541 gacaagatct gctttttgct tgtactgggt ctctctggtt agaccagatc tgagcctggg
     6601 agctctctgg ctaactaggg aacccactgc ttaagcctca ataaagcttg ccttgagtgc
     6661 ttcaagtagt gtgtgcccgt ctgttgtgtg actctggtaa ctagagatcc ctcagaccct
     6721 tttagtcagt gtggaaaatc tctagcagca tctagaatta attccgtgta ttctatagtg
     6781 tcacctaaat cgtatgtgta tgatacataa ggttatgtat taattgtagc cgcgttctaa
     6841 cgacaatatg tacaagccta attgtgtagc atctggctta ctgaagcaga ccctatcatc
     6901 tctctcgtaa actgccgtca gagtcggttt ggttggacga accttctgag tttctggtaa
     6961 cgccgtcccg cacccggaaa tggtcagcga accaatcagc agggtcatcg ctagccagat
     7021 cctctacgcc ggacgcatcg tggccggcat caccggcgcc acaggtgcgg ttgctggcgc
     7081 ctatatcgcc gacatcaccg atggggaaga tcgggctcgc cacttcgggc tcatgagcgc
     7141 ttgtttcggc gtgggtatgg tggcaggccc cgtggccggg ggactgttgg gcgccatctc
     7201 cttgcatgca ccattccttg cggcggcggt gctcaacggc ctcaacctac tactgggctg
     7261 cttcctaatg caggagtcgc ataagggaga gcgtcgaatg gtgcactctc agtacaatct
     7321 gctctgatgc cgcatagtta agccagcccc gacacccgcc aacacccgct gacgcgccct
     7381 gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct
     7441 gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gagacgaaag ggcctcgtga
     7501 tacgcctatt tttataggtt aatgtcatga taataatggt ttcttagacg tcaggtggca
     7561 cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata
     7621 tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga
     7681 gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc
     7741 ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg
     7801 cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc
     7861 ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat
     7921 cccgtattga cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact
     7981 tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat
     8041 tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga
     8101 tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc
     8161 ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga
     8221 tgcctgtagc aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag
     8281 cttcccggca acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc
     8341 gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt
     8401 ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct
     8461 acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg
     8521 cctcactgat taagcattgg taactgtcag accaagttta ctcatatata ctttagattg
     8581 atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca
     8641 tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga
     8701 tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa
     8761 aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga
     8821 aggtaactgg cttcagcaga gcgcagatac caaatactgt tcttctagtg tagccgtagt
     8881 taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt
     8941 taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat
     9001 agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct
     9061 tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca
     9121 cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag
     9181 agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc
     9241 gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga
     9301 aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca
     9361 tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag
     9421 ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg
     9481 aagagcgccc aatacgcaaa ccgcctctcc ccgcgcgttg gccgattcat taatgcagct
     9541 gtggaatgtg tgtcagttag ggtgtggaaa gtccccaggc tccccagcag gcagaagtat
     9601 gcaaagcatg catctcaatt agtcagcaac caggtgtgga aagtccccag gctccccagc
     9661 aggcagaagt atgcaaagca tgcatctcaa ttagtcagca accatagtcc cgcccctaac
     9721 tccgcccatc ccgcccctaa ctccgcccag ttccgcccat tctccgcccc atggctgact
     9781 aatttttttt atttatgcag aggccgaggc cgcctcggcc tctgagctat tccagaagta
     9841 gtgaggaggc ttttttggag gcctaggctt ttgcaaaaag cttggacaca agacaggctt
     9901 gcgagatatg tttgagaata ccactttatc ccgcgtcagg gagaggcagt gcgtaaaaag
     9961 acgcggactc atgtgaaata ctggttttta gtgcgccaga tctctataat ctcgcgcaac
    10021 ctattttccc ctcgaacact ttttaagccg tagataaaca ggctgggaca cttcacatga
    10081 gcgaaaaata catcgtcacc tgggacatgt tgcagatcca tgcacgtaaa ctcgcaagcc
    10141 gactgatgcc ttctgaacaa tggaaaggca ttattgccgt aagccgtggc ggtctgtacc
    10201 gggtgcgtta ctggcgcgtg aactgggtat tcgtcatgtc gataccgttt gtatttccag
    10261 ctacgatcac gacaaccagc gcgagcttaa agtgctgaaa cgcgcagaag gcgatggcga
    10321 aggcttcatc gttattgatg acctggtgga taccggtggt actgcggttg cgattcgtga
    10381 aatgtatcca aaagcgcact ttgtcaccat cttcgcaaaa ccggctggtc gtccgctggt
    10441 tgatgactat gttgttgata tcccgcaaga tacctggatt gaacagccgt gggatatggg
    10501 cgtcgtattc gtcccgccaa tctccggtcg ctaatctttt caacgcctgg cactgccggg
    10561 cgttgttctt tttaacttca ggcgggttac aatagtttcc agtaagtatt ctggaggctg
    10621 catccatgac acaggcaaac ctgagcgaaa ccctgttcaa accccgcttt aaacatcctg
    10681 aaacctcgac gctagtccgc cgctttaatc acggcgcaca accgcctgtg cagtcggccc
    10741 ttgatggtaa aaccatccct cactggtatc gcatgattaa ccgtctgatg tggatctggc
    10801 gcggcattga cccacgcgaa atcctcgacg tccaggcacg tattgtgatg agcgatgccg
    10861 aacgtaccga cgatgattta tacgatacgg tgattggcta ccgtggcggc aactggattt
    10921 atgagtgggc cccggatctt tgtgaaggaa ccttacttct gtggtgtgac ataattggac
    10981 aaactaccta cagagattta aagctctaag gtaaatataa aatttttaag tgtataatgt
    11041 gttaaactac tgattctaat tgtttgtgta ttttagattc caacctatgg aactgatgaa
    11101 tgggagcagt ggtggaatgc ctttaatgag gaaaacctgt tttgctcaga agaaatgcca
    11161 tctagtgatg atgaggctac tgctgactct caacattcta ctcctccaaa aaagaagaga
    11221 aaggtagaag accccaagga ctttccttca gaattgctaa gttttttgag tcatgctgtg
    11281 tttagtaata gaactcttgc ttgctttgct atttacacca caaaggaaaa agctgcactg
    11341 ctatacaaga aaattatgga aaaatattct gtaaccttta taagtaggca taacagttat
    11401 aatcataaca tactgttttt tcttactcca cacaggcata gagtgtctgc tattaataac
    11461 tatgctcaaa aattgtgtac ctttagcttt ttaatttgta aaggggttaa taaggaatat
    11521 ttgatgtata gtgccttgac tagagatcat aatcagccat accacatttg tagaggtttt
    11581 acttgcttta aaaaacctcc cacacctccc cctgaacctg aaacataaaa tgaatgcaat
    11641 tgttgttgtt aacttgttta ttgcagctta taatggttac aaataaagca atagcatcac
    11701 aaatttcaca aataaagcat ttttttcact gcattctagt tgtggtttgt ccaaactcat
    11761 caatgtatct tatcatgtct ggatcaactg gataactcaa gctaaccaaa atcatcccaa
    11821 acttcccacc ccatacccta ttaccactgc caattacctg tggtttcatt tactctaaac
    11881 ctgtgattcc tctgaattat tttcatttta aagaaattgt atttgttaaa tatgtactac
    11941 aaacttagta gt
//
