LOCUS       sequence-212638-       12878 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Donor vector for targeting H2B-miRFP703 into Rosa26 locus to 
            generate reporter mice or cell lines.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12878)
  AUTHORS   Gu B, Posfai E, Rossant J
  TITLE     Efficient generation of targeted large insertions by microinjection 
            into two-cell-stage mouse embryos.
  JOURNAL   Nat Biotechnol. 2018 Jun 11. pii: nbt.4166. doi: 10.1038/nbt.4166.
  PUBMED    29889212
REFERENCE   2  (bases 1 to 12878)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Biotechnol. 2018 Jun 11. pii: nbt.4166. doi: 10.1038/nbt.4166."
COMMENT     Sequence Label: pR26-CAG-H2B-miRFP703
FEATURES             Location/Qualifiers
     source          1..12878
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    25..1107
                     /label=Rosa26 left arm
                     /note="left homology arm from intron 1 of the mouse Rosa26 
                     gene (Soriano, 1999)"
     misc_feature    join(1130..1195,1196..1233)
                     /label=Ad SA
                     /note="adenovirus major late transcript splice acceptor 
                     (Friedrich and Soriano, 1991)"
     intron          1477..1542
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     polyA_signal    1589..1723
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     protein_bind    1855..1902
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     enhancer        1917..2296
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2299..2574
                     /label=chicken beta-actin promoter
     intron          2575..3583
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     3507..3529
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     protein_bind    complement(3630..3663)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     CDS             3749..4126
                     /codon_start=1
                     /gene="HIST1H2BJ"
                     /product="human histone H2B"
                     /label=H2B
                     /translation="MPEPAKSAPAPKKGSKKAVTKAQKKGGKKRKRSRKESYSIYVYKV
                     LKQVHPDTGISSKAMGIMNSFVNDIFERIAGEASRLAHYNKRSTITSREIQTAVRLLLP
                     GELAKHAVSEGTKAITKYTSAK"
     regulatory      4139..4148
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             4145..5092
                     /codon_start=1
                     /product="bright monomeric near-infrared fluorescent 
                     protein engineered from a bacterial phytochrome, with an 
                     emission maximum of 703 nm (Shcherbakova et al., 2016)"
                     /label=miRFP703
                     /label=ORF frame 1
                     /translation="MVAGHASGSPAFGTASHSNCEHEEIHLAGSIQPHGALLVVSEHDH
                     RVIQASANAAEFLNLGSVLGVPLAEIDGDLLIKILPHLDPTAEGMPVAVRCRIGNPSTE
                     YCGLMHRPPEGGLIIELERAGPSIDLSGTLAPALERIRTAGSLRALCDDTVLLFQQCTG
                     YDRVMVYRFDEQGHGLVFSECHVPGLESYFGNRYPSSLVPQMARQLYVRQRVRVLVDVT
                     YQPVPLEPRLSPLTGRDLDMSGCFLRSMSPIHLQFLKDMGVRATLAVSLVVGGKLWGLV
                     VCHHYLPRFIRFELRAICKRLAERIATRITALES"
     primer_bind     complement(5110..5126)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5110..5126)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     primer_bind     complement(5140..5157)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    5146..5370
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     polyA_signal    5436..5570
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     misc_feature    5643..9983
                     /label=Rosa26 right arm
                     /note="right homology arm from intron 1 of the mouse Rosa26
                     gene (Soriano, 1999)"
     primer_bind     complement(10033..10053)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(10033..10051)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(10072..10088)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(10072..10088)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(10085..10107)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    10096..10112
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(10120..10126,10127..10144,10145..10150))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    10165..10186
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(10303..10320)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(10474..11062)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(10554..10573)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(11233..12024,12025..12093))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     11856..11875
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(12094..12198)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(12224..12679)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(12361..12382)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     12573..12592
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     12806..12828
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     12820..12837
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     12821..12837
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        12847..12865
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 caccgcatta ccctgttatc cctagcggca ggccctccga gcgtggtgga gccgttctgt
       61 gagacagccg ggtacgagtc gtgacgctgg aaggggcaag cgggtggtgg gcaggaatgc
      121 ggtccgccct gcagcaaccg gagggggagg gagaagggag cggaaaagtc tccaccggac
      181 gcggccatgg ctcggggggg ggggggcagc ggaggagcgc ttccggccga cgtctcgtcg
      241 ctgattggct tcttttcctc ccgccgtgtg tgaaaacaca aatggcgtgt tttggttggc
      301 gtaaggcgcc tgtcagttaa cggcagccgg agtgcgcagc cgccggcagc ctcgctctgc
      361 ccactgggtg gggcgggagg taggtggggt gaggcgagct ggacgtgcgg gcgcggtcgg
      421 cctctggcgg ggcgggggag gggagggagg gtcagcgaaa gtagctcgcg cgcgagcggc
      481 cgcccaccct ccccttcctc tgggggagtc gttttacccg ccgccggccg ggcctcgtcg
      541 tctgattggc tctcggggcc cagaaaactg gcccttgcca ttggctcgtg ttcgtgcaag
      601 ttgagtccat ccgccggcca gcgggggcgg cgaggaggcg ctcccaggtt ccggccctcc
      661 cctcggcccc gcgccgcaga gtctggccgc gcgcccctgc gcaacgtggc aggaagcgcg
      721 cgctgggggc ggggacgggc agtagggctg agcggctgcg gggcgggtgc aagcacgttt
      781 ccgacttgag ttgcctcaag aggggcgtgc tgagccagac ctccatcgcg cactccgggg
      841 agtggaggga aggagcgagg gctcagttgg gctgttttgg aggcaggaag cacttgctct
      901 cccaaagtcg ctctgagttg ttatcagtaa gggagctgca gtggagtagg cggggagaag
      961 gccgcaccct tctccggagg ggggagggga gtgttgcaat acctttctgg gagttctctg
     1021 ctgcctcctg gcttctgagg accgccctgg gcctgggaga atcccttccc cctcttccct
     1081 cgtgatctgc aactccagtc tttctaggcg cgcccgggct gcagatctgt agggcgcagt
     1141 agtccagggt ttccttgatg atgtcatact tatcctgtcc cttttttttc cacagctcgc
     1201 ggttgaggac aaactcttcg cggtctttcc agtggggatc gacggtatcg ataagctggc
     1261 cgctctagtg gccgtacggg cccacctgcc gggccactta attaaattta aatcacaatt
     1321 ccagctgagc gccggtcgct accattacca gttggtctgg tgtcaaaaat aataataacc
     1381 gggcaggggg gatctgcatg gatctttgtg aaggaacctt acttctgtgg tgtgacataa
     1441 ttggacaaac tacctacaga gatttaaagc tctaaggtaa atataaaatt tttaagtgta
     1501 taatgtgtta aactactgat tctaattgtt tgtgtatttt agattccaac ctatggaact
     1561 gatgaatggg agcagtggtg gaatgccaga tccagacatg ataagataca ttgatgagtt
     1621 tggacaaacc acaactagaa tgcagtgaaa aaaatgcttt atttgtgaaa tttgtgatgc
     1681 tattgcttta tttgtaacca ttataagctg caataaacaa gttaacaaca acaattgcat
     1741 tcattttatg tttcaggttc agggggaggt gtgggaggtt ttttaaagca agtaaaacct
     1801 ctacaaatgt ggtatggctg attatgatct gcggccgtgc tagcgcttaa gcttgaagtt
     1861 cctattccga agttcctatt ctctagaaag tataggaact tcggcgcgcc ggatctgaca
     1921 ttgattattg actagttatt aatagtaatc aattacgggg tcattagttc atagcccata
     1981 tatggagttc cgcgttacat aacttacggt aaatggcccg cctggctgac cgcccaacga
     2041 cccccgccca ttgacgtcaa taatgacgta tgttcccata gtaacgccaa tagggacttt
     2101 ccattgacgt caatgggtgg agtatttacg gtaaactgcc cacttggcag tacatcaagt
     2161 gtatcatatg ccaagtacgc cccctattga cgtcaatgac ggtaaatggc ccgcctggca
     2221 ttatgcccag tacatgacct tatgggactt tcctacttgg cagtacatct acgtattagt
     2281 catcgctatt accatgggtc gaggtgagcc ccacgttctg cttcactctc cccatctccc
     2341 ccccctcccc acccccaatt ttgtatttat ttatttttta attattttgt gcagcgatgg
     2401 gggcgggggg ggggggggcg cgcgccaggc ggggcggggc ggggcgaggg gcggggcggg
     2461 gcgaggcgga gaggtgcggc ggcagccaat cagagcggcg cgctccgaaa gtttcctttt
     2521 atggcgaggc ggcggcggcg gcggccctat aaaaagcgaa gcgcgcggcg ggcgggagtc
     2581 gctgcgttgc cttcgccccg tgccccgctc cgcgccgcct cgcgccgccc gccccggctc
     2641 tgactgaccg cgttactccc acaggtgagc gggcgggacg gcccttctcc tccgggctgt
     2701 aattagcgct tggtttaatg acggctcgtt tcttttctgt ggctgcgtga aagccttaaa
     2761 gggctccggg agggcccttt gtgcgggggg gagcggctcg gggggtgcgt gcgtgtgtgt
     2821 gtgcgtgggg agcgccgcgt gcggcccgcg ctgcccggcg gctgtgagcg ctgcgggcgc
     2881 ggcgcggggc tttgtgcgct ccgcgtgtgc gcgaggggag cgcggccggg ggcggtgccc
     2941 cgcggtgcgg gggggctgcg aggggaacaa aggctgcgtg cggggtgtgt gcgtgggggg
     3001 gtgagcaggg ggtgtgggcg cggcggtcgg gctgtaaccc ccccctgcac ccccctcccc
     3061 gagttgctga gcacggcccg gcttcgggtg cggggctccg tgcggggcgt ggcgcggggc
     3121 tcgccgtgcc gggcgggggg tggcggcagg tgggggtgcc gggcggggcg gggccgcctc
     3181 gggccgggga gggctcgggg gaggggcgcg gcggccccgg agcgccggcg gctgtcgagg
     3241 cgcggcgagc cgcagccatt gccttttatg gtaatcgtgc gagagggcgc agggacttcc
     3301 tttgtcccaa atctggcgga gccgaaatct gggaggcgcc gccgcacccc ctctagcggg
     3361 cgcgggcgaa gcggtgcggc gccggcagga aggaaatggg cggggagggc cttcgtgcgt
     3421 cgccgcgccg ccgtcccctt ctccatctcc agcctcgggg ctgccgcagg gggacggctg
     3481 ccttcggggg ggacggggca gggcggggtt cggcttctgg cgtgtgaccg gcggctctag
     3541 agcctctgct aaccatgttc atgccttctt ctttttccta cagatcctta attaaccgtc
     3601 ttaagcctgt aacaaccggt acagttcgaa taacttcgta tagcatacat tatacgaagt
     3661 tataagctta ccgggccacc atagtcgcga gtagcttggg ccagctaggc cttgaccaaa
     3721 gttcctctgg aattgcgatc gcacgcgtat gccagagcca gcgaagtctg ctcccgcccc
     3781 gaaaaagggc tccaagaagg cggtgactaa ggcgcagaag aaaggcggca agaagcgcaa
     3841 gcgcagccgc aaggagagct attccatcta tgtgtacaag gttctgaagc aggtccaccc
     3901 tgacaccggc atttcgtcca aggccatggg catcatgaat tcgtttgtga acgacatttt
     3961 cgagcgcatc gcaggtgagg cttcccgcct ggcgcattac aacaagcgct cgaccatcac
     4021 ctccagggag atccagacgg ccgtgcgcct gctgctgcct ggggagttgg ccaagcacgc
     4081 cgtgtccgag ggtactaagg ccatcaccaa gtacaccagc gctaaggatc caccggtcgc
     4141 caccatggta gcaggtcatg cctctggcag ccccgcattc gggaccgcct ctcattcgaa
     4201 ttgcgaacat gaagagatcc acctcgccgg ctcgatccag ccgcatggcg cgcttctggt
     4261 cgtcagcgaa catgatcatc gcgtcatcca ggccagcgcc aacgccgcgg aatttctgaa
     4321 tctcggaagc gtactcggcg ttccgctcgc cgagatcgac ggcgatctgt tgatcaagat
     4381 cctgccgcat ctcgatccca ccgccgaagg catgccggtc gcggtgcgct gccggatcgg
     4441 caatccctct acggagtact gcggtctgat gcatcggcct ccggaaggcg ggctgatcat
     4501 cgaactcgaa cgtgccggcc cgtcgatcga tctgtcaggc acgctggcgc cggcgctgga
     4561 gcggatccgc acggcgggtt cactgcgcgc gctgtgcgat gacaccgtgc tgctgtttca
     4621 gcagtgcacc ggctacgacc gggtgatggt gtatcgtttc gatgagcaag gccacggcct
     4681 ggtattctcc gagtgccatg tgcctgggct cgaatcctat ttcggcaacc gctatccgtc
     4741 gtcgctggtc ccgcagatgg cgcggcagct gtacgtgcgg cagcgcgtcc gcgtgctggt
     4801 cgacgtcacc tatcagccgg tgccgctgga gccgcggctg tcgccgctga ccgggcgcga
     4861 tctcgacatg tcgggctgct tcctgcgctc gatgtcgccg atccatctgc agttcctgaa
     4921 ggacatgggc gtgcgcgcca ccctggcggt gtcgctggtg gtcggcggca agctgtgggg
     4981 cctggttgtc tgtcaccatt atctgccgcg cttcatccgt ttcgagctgc gggcgatctg
     5041 caaacggctc gccgaaagga tcgcgacgcg gatcaccgcg cttgagagct aaacgcgtaa
     5101 atgattgcag atccactagt tctagagctc gctgatcagc ctcgactgtg ccttctagtt
     5161 gccagccatc tgttgtttgc ccctcccccg tgccttcctt gaccctggaa ggtgccactc
     5221 ccactgtcct ttcctaataa aatgaggaaa ttgcatcgca ttgtctgagt aggtgtcatt
     5281 ctattctggg gggtggggtg gggcaggaca gcaaggggga ggattgggaa gacaatagca
     5341 ggcatgctgg ggatgcggtg ggctctatgg cttctgaggc ggaaagaacc agctggggct
     5401 cgagcctcta gaactatagt gagtcgtatt acgtagatcc agacatgata agatacattg
     5461 atgagtttgg acaaaccaca actagaatgc agtgaaaaaa atgctttatt tgtgaaattt
     5521 gtgatgctat tgctttattt gtaaccatta taagctgcaa taaacaagtt aacaacaaca
     5581 attgcattca ttttatgttt caggttcagg gggaggtgtg ggaggttttt taattcgcgg
     5641 ccctagaaga tgggcgggag tcttctgggc aggcttaaag gctaacctgg tgtgtgggcg
     5701 ttgtcctgca ggggaattga acaggtgtaa aattggaggg acaagacttc ccacagattt
     5761 tcggttttgt cgggaagttt tttaataggg gcaaataagg aaaatgggag gataggtagt
     5821 catctggggt tttatgcagc aaaactacag gttattattg cttgtgatcc gcctcggagt
     5881 attttccatc gaggtagatt aaagacatgc tcacccgagt tttatactct cctgcttgag
     5941 atccttacta cagtatgaaa ttacagtgtc gcgagttaga ctatgtaagc agaattttaa
     6001 tcatttttaa agagcccagt acttcatatc catttctccc gctccttctg cagccttatc
     6061 aaaaggtatt ttagaacact cattttagcc ccattttcat ttattatact ggcttatcca
     6121 acccctagac agagcattgg cattttccct ttcctgatct tagaagtctg atgactcatg
     6181 aaaccagaca gattagttac atacaccaca aatcgaggct gtagctgggg cctcaacact
     6241 gcagttcttt tataactcct tagtacactt tttgttgatc ctttgccttg atccttaatt
     6301 ttcagtgtct atcacctctc ccgtcaggtg gtgttccaca tttgggccta ttctcagtcc
     6361 agggagtttt acaacaatag atgtattgag aatccaacct aaagcttaac tttccactcc
     6421 catgaatgcc tctctccttt ttctccattt ataaactgag ctattaacca ttaatggttt
     6481 ccaggtggat gtctcctccc ccaatattac ctgatgtatc ttacatattg ccaggctgat
     6541 attttaagac attaaaaggt atatttcatt attgagccac atggtattga ttactgctta
     6601 ctaaaatttt gtcattgtac acatctgtaa aaggtggttc cttttggaat gcaaagttca
     6661 ggtgtttgtt gtctttcctg acctaaggtc ttgtgagctt gtattttttc tatttaagca
     6721 gtgctttctc ttggactggc ttgactcatg gcattctaca cgttattgct ggtctaaatg
     6781 tgattttgcc aagcttcttc aggacctata attttgcttg acttgtagcc aaacacaagt
     6841 aaaatgatta agcaacaaat gtatttgtga agcttggttt ttaggttgtt gtgttgtgtg
     6901 tgcttgtgct ctataataat actatccagg ggctggagag gtggctcgga gttcaagagc
     6961 acagactgct cttccagaag tcctgagttc aattcccagc aaccacatgg tggctcacaa
     7021 ccatctgtaa tgggatctga tgccctcttc tggtgtgtct gaagaccaca agtgtattca
     7081 cattaaataa ataaatcctc cttcttcttc tttttttttt ttttaaagag aatactgtct
     7141 ccagtagaat ttactgaagt aatgaaatac tttgtgtttg ttccaatatg gtagccaata
     7201 atcaaattac tctttaagca ctggaaatgt taccaaggaa ctaattttta tttgaagtgt
     7261 aactgtggac agaggagcca taactgcaga cttgtgggat acagaagacc aatgcagact
     7321 ttaatgtctt ttctcttaca ctaagcaata aagaaataaa aattgaactt ctagtatcct
     7381 atttgtttaa actgctagct ttacttaact tttgtgcttc atctatacaa agctgaaagc
     7441 taagtctgca gccattacta aacatgaaag caagtaatga taattttgga tttcaaaaat
     7501 gtagggccag agtttagcca gccagtggtg gtgcttgcct ttatgccttt aatcccagca
     7561 ctctggaggc agagacaggc agatctctga gtttgagccc agcctggtct acacatcaag
     7621 ttctatctag gatagccagg aatacacaca gaaaccctgt tggggagggg ggctctgaga
     7681 tttcataaaa ttataattga agcattccct aatgagccac tatggatgtg gctaaatccg
     7741 tctacctttc tgatgagatt tgggtattat tttttctgtc tctgctgttg gttgggtctt
     7801 ttgacactgt gggctttctt taaagcctcc ttcctgccat gtggtctctt gtttgctact
     7861 aacttcccat ggcttaaatg gcatggcttt ttgccttcta agggcagctg ctgagatttg
     7921 cagcctgatt tccagggtgg ggttgggaaa tctttcaaac actaaaattg tcctttaatt
     7981 ttttttttaa aaaatgggtt atataataaa cctcataaaa tagttatgag gagtgaggtg
     8041 gactaatatt aaatgagtcc ctcccctata aaagagctat taaggctttt tgtcttatac
     8101 ttaacttttt ttttaaatgt ggtatcttta gaaccaaggg tcttagagtt ttagtataca
     8161 gaaactgttg catcgcttaa tcagattttc tagtttcaaa tccagagaat ccaaattctt
     8221 cacagccaaa gtcaaattaa gaatttctga cttttaatgt taatttgctt actgtgaata
     8281 taaaaatgat agcttttcct gaggcagggt ctcactatgt atctctgcct gatctgcaac
     8341 aagatatgta gactaaagtt ctgcctgctt ttgtctcctg aatactaagg ttaaaatgta
     8401 gtaatacttt tggaacttgc aggtcagatt cttttatagg ggacacacta agggagcttg
     8461 ggtgatagtt ggtaaaatgt gtttcaagtg atgaaaactt gaattattat caccgcaacc
     8521 tactttttaa aaaaaaaagc caggcctgtt agagcatgct taagggatcc ctaggacttg
     8581 ctgagcacac aagagtagtt acttggcagg ctcctggtga gagcatattt caaaaaacaa
     8641 ggcagacaac caagaaacta cagttaaggt tacctgtctt taaaccatct gcatatacac
     8701 agggatatta aaatattcca aataatattt cattcaagtt ttcccccatc aaattgggac
     8761 atggatttct ccggtgaata ggcagagttg gaaactaaac aaatgttggt tttgtgattt
     8821 gtgaaattgt tttcaagtga tagttaaagc ccatgagata cagaacaaag ctgctatttc
     8881 gaggtctctt ggtttatact cagaagcact tctttgggtt tccctgcact atcctgatca
     8941 tgtgctaggc ctaccttagg ctgattgttg ttcaaataaa cttaagtttc ctgtcaggtg
     9001 atgtcatatg atttcatata tcaaggcaaa acatgttata tatgttaaac atttgtactt
     9061 aatgtgaaag ttaggtcttt gtgggtttga tttttaattt tcaaaacctg agctaaataa
     9121 gtcattttta catgtcttac atttggtgga attgtataat tgtggtttgc aggcaagact
     9181 ctctgaccta gtaaccctac ctatagagca ctttgctggg tcacaagtct aggagtcaag
     9241 catttcacct tgaagttgag acgttttgtt agtgtatact agtttatatg ttggaggaca
     9301 tgtttatcca gaagatattc aggactattt ttgactgggc taaggaattg attctgatta
     9361 gcactgttag tgagcattga gtggccttta ggcttgaatt ggagtcactt gtatatctca
     9421 aataatgctg gcctttttta aaaagccctt gttctttatc accctgtttt ctacataatt
     9481 tttgttcaaa gaaatacttg tttggatctc cttttgacaa caatagcatg ttttcaagcc
     9541 atattttttt tccttttttt tttttttttt ggtttttcga gacagggttt ctctgtatag
     9601 ccctggctgt cctggaactc actttgtaga ccaggctggc ctcgaactca gaaatccgcc
     9661 tgcctctgcc tcctgagtgc cgggattaaa ggcgtgcacc accacgcctg gctaagttgg
     9721 atattttgtt atataactat aaccaatact aactccactg ggtggatttt taattcagtc
     9781 agtagtctta agtggtcttt attggccctt cattaaaatc tactgttcac tctaacagag
     9841 gctgttggta ctagtggcac ttaagcaact tcctacggat atactagcag attaagggtc
     9901 agggatagaa actagtctag cgttttgtat acctaccagc tttatactac cttgttctga
     9961 tagaaatatt tcaggacatc tagcttatcg ataccgtcga cggtatcgat aagcttgatc
    10021 cagcttttgt tccctttagt gagggttaat tgcgcgcttg gcgtaatcat ggtcatagct
    10081 gtttcctgtg tgaaattgtt atccgctcac aattccacac aacatacgag ccggaagcat
    10141 aaagtgtaaa gcctggggtg cctaatgagt gagctaactc acattaattg cgttgcgctc
    10201 actgcccgct ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa tcggccaacg
    10261 cgcggggaga ggcggtttgc gtattgggcg ctcttccgct tcctcgctca ctgactcgct
    10321 gcgctcggtc gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg taatacggtt
    10381 atccacagaa tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc
    10441 caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga
    10501 gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata
    10561 ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac
    10621 cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg
    10681 taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc
    10741 cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag
    10801 acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt
    10861 aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta gaaggacagt
    10921 atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg
    10981 atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac
    11041 gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca
    11101 gtggaacgaa aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac
    11161 ctagatcctt ttaaattaaa aatgaagttt taaatcaatc taaagtatat atgagtaaac
    11221 ttggtctgac agttaccaat gcttaatcag tgaggcacct atctcagcga tctgtctatt
    11281 tcgttcatcc atagttgcct gactccccgt cgtgtagata actacgatac gggagggctt
    11341 accatctggc cccagtgctg caatgatacc gcgagaccca cgctcaccgg ctccagattt
    11401 atcagcaata aaccagccag ccggaagggc cgagcgcaga agtggtcctg caactttatc
    11461 cgcctccatc cagtctatta attgttgccg ggaagctaga gtaagtagtt cgccagttaa
    11521 tagtttgcgc aacgttgttg ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg
    11581 tatggcttca ttcagctccg gttcccaacg atcaaggcga gttacatgat cccccatgtt
    11641 gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt gtcagaagta agttggccgc
    11701 agtgttatca ctcatggtta tggcagcact gcataattct cttactgtca tgccatccgt
    11761 aagatgcttt tctgtgactg gtgagtactc aaccaagtca ttctgagaat agtgtatgcg
    11821 gcgaccgagt tgctcttgcc cggcgtcaat acgggataat accgcgccac atagcagaac
    11881 tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga aaactctcaa ggatcttacc
    11941 gctgttgaga tccagttcga tgtaacccac tcgtgcaccc aactgatctt cagcatcttt
    12001 tactttcacc agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg
    12061 aataagggcg acacggaaat gttgaatact catactcttc ctttttcaat attattgaag
    12121 catttatcag ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa
    12181 acaaataggg gttccgcgca catttccccg aaaagtgcca cctaaattgt aagcgttaat
    12241 attttgttaa aattcgcgtt aaatttttgt taaatcagct cattttttaa ccaataggcc
    12301 gaaatcggca aaatccctta taaatcaaaa gaatagaccg agatagggtt gagtgttgtt
    12361 ccagtttgga acaagagtcc actattaaag aacgtggact ccaacgtcaa agggcgaaaa
    12421 accgtctatc agggcgatgg cccactacgt gaaccatcac cctaatcaag ttttttgggg
    12481 tcgaggtgcc gtaaagcact aaatcggaac cctaaaggga gcccccgatt tagagcttga
    12541 cggggaaagc cggcgaacgt ggcgagaaag gaagggaaga aagcgaaagg agcgggcgct
    12601 agggcgctgg caagtgtagc ggtcacgctg cgcgtaacca ccacacccgc cgcgcttaat
    12661 gcgccgctac agggcgcgtc ccattcgcca ttcaggctgc gcaactgttg ggaagggcga
    12721 tcggtgcggg cctcttcgct attacgccag ctggcgaaag ggggatgtgc tgcaaggcga
    12781 ttaagttggg taacgccagg gttttcccag tcacgacgtt gtaaaacgac ggccagtgag
    12841 cgcgcgtaat acgactcact atagggcgaa ttggagct
//
