LOCUS       sequence-212641-        1320 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  For tetracycline-inducible expression of bio-myc-NES-EGFP in 
            mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1320)
  AUTHORS   Trotman JB, Agana BA, Giltmier AJ, Wysocki VH, Schoenberg DR
  TITLE     RNA-binding proteins and heat-shock protein 90 are constituents of 
            the cytoplasmic capping enzyme interactome.
  JOURNAL   J Biol Chem. 2018 Oct 26;293(43):16596-16607. doi: 
            10.1074/jbc.RA118.004973. Epub 2018 Aug 30.
  PUBMED    30166341
REFERENCE   2  (bases 1 to 1320)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1074/jbc.RA118.004973"; journalName: "J Biol Chem"; date: 
            "2018-10-26- 26"; volume: "293"; issue: "43"; pages: "16596-16607"
COMMENT     Sequence Label: pcDNA4/TO-bio-myc-NES-EGFP
FEATURES             Location/Qualifiers
     source          1..1320
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             454..480
                     /codon_start=1
                     /product="nuclear export signal from the HIV Rev protein 
                     (Fischer et al., 1995)"
                     /label=NES
                     /translation="LPPLERLTL"
     CDS             join(499..501,502..504,505..1215)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(805..824)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     1152..1173
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 ttagtgaacc gtcagatcgc ctggagacgc catccacgct gttttgacct ccatagaaga
       61 caccgggacc gatccagcct ccggactcta gcgtttaaac ttaagcttgg taccatggag
      121 ttaaaaaccg ccgcactagc tcaacatgac gaggctgctg gaaaggccgg tgaaggtgaa
      181 atccctgccc ctcttgctgg taccgtttct aagatactgg taaaagaagg tgacactgtt
      241 aaagctggtc aaacagttct ggtgctggag gctatgaaaa tggagacaga aattaacgct
      301 cctactgacg gaaaagttga aaaggtgtta gttaaggaaa gagatgctgt tcaaggtggt
      361 caaggtctaa tcaagatcgg cgttggcacc atggcatcaa tgcagaagct gatctcagag
      421 gaggacctgc ttatggccat ggaggccctt cagctaccac cgcttgagag acttactctt
      481 gatatggcca tggaggccat ggtgagtaag ggggaggaac tctttacagg cgtcgtgccg
      541 attttggtag agctggacgg cgacgtaaac ggccacaagt tcagcgtgtc cggcgagggc
      601 gagggcgatg ccacctacgg caagctgacc ctgaagttca tctgcaccac cggcaagctg
      661 cccgtgccct ggcccaccct cgtgaccacc ctgacctacg gcgtgcagtg cttcagccgc
      721 taccccgacc acatgaagca gcacgacttc ttcaagtccg ccatgcccga aggctacgtc
      781 caggagcgca ccatcttctt caaggacgac ggcaactaca agacccgcgc cgaggtgaag
      841 ttcgagggcg acaccctggt gaaccgcatc gagctgaagg gcatcgactt caaggaggac
      901 ggcaacatcc tggggcacaa gctggagtac aactacaaca gccacaacgt ctatatcatg
      961 gccgacaagc agaagaacgg catcaaggtg aacttcaaga tccgccacaa catcgaggac
     1021 ggcagcgtgc agctcgccga ccactaccag cagaacaccc ccatcggcga cggccccgtg
     1081 ctgctgcccg acaaccacta cctgagcacc cagtccgccc tgagcaaaga ccccaacgag
     1141 aagcgcgatc acatggtcct gctggagttc gtgaccgccg ccgggatcac tctcggcatg
     1201 gacgagctgt acaagtgatg aggtaccgag ctcggatcca ctagtccagt gtggtggaat
     1261 tctgcagata tccagcacag tggcggccgc tcgagtctag agggcccgtt taaacccgct
//
