LOCUS       sequence-212657-       11617 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  For the nuclear localization of the PixELL system.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11617)
  AUTHORS   Dine E, Gil AA, Uribe G, Brangwynne CP, Toettcher JE
  TITLE     Protein Phase Separation Provides Long-Term Memory of Transient 
            Spatial Stimuli.
  JOURNAL   Cell Syst. 2018 May 24. pii: S2405-4712(18)30190-X. doi: 
            10.1016/j.cels.2018.05.002.
  PUBMED    29859829
REFERENCE   2  (bases 1 to 11617)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Syst. 
            2018 May 24. pii: S2405-4712(18)30190-X. doi: 
            10.1016/j.cels.2018.05.002."
COMMENT     Sequence Label: NLS-FUS-Citrine-PixE
FEATURES             Location/Qualifiers
     source          1..11617
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             1..634
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    681..806
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1303..1536
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1721..1765
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2050..2167
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2320..2727
                     /label=SFFV promoter
                     /note="spleen focus-forming virus long terminal repeat 
                     (LTR) promoter"
     CDS             2801..2821
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     protein_bind    complement(2826..2850)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             3241..3252
                     /codon_start=1
                     /product="WELQut protease recognition and cleavage site"
                     /label=WELQut site
                     /translation="WELQ"
     CDS             join(3512..3514,3515..3517,3518..4228)
                     /codon_start=1
                     /product="enhanced variant of YFP (Heikal et al., 2001)"
                     /label=Citrine
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTFGYGLMCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(3557..3578)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(3818..3837)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     4165..4186
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     misc_feature    5481..6069
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(5534..5554)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(5952..5963)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(6072..6088)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6073..6089)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             6179..6412
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     promoter        complement(6437..6455)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(6438..6455)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(6745..6764)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     complement(6978..6997)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     7097..7119
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(7157..7175)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        7243..7347
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7348..7416,7417..8208)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7566..7585)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8379..8967
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8868..8887
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     9121..9138
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(9208..9228)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        9213..9542
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      9393..9528
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     intron          10676..10741
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             10871..10891
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    11316..11450
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(11353..11372)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     11407..11426
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
ORIGIN
        1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
       61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
      121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
      181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
      241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
      301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
      361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
      421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
      481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
      541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
      601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
      661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
      721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
      781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
      841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
      901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
      961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
     1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
     1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
     1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
     1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
     1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
     1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
     1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
     1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
     1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
     1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
     1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
     1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
     1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
     1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
     1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
     1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
     1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccaaa tggcagtatt
     2041 catccacaat tttaaaagaa aaggggggat tggggggtac agtgcagggg aaagaatagt
     2101 agacataata gcaacagaca tacaaactaa agaattacaa aaacaaatta caaaaattca
     2161 aaattttcgg gtttattaca gggacagcag agatccagtt tggatcgata agcttgatat
     2221 cgaattcctg cagccccgat aaaataaaag attttattta gtctccagaa aaagggggga
     2281 atgaaagacc ccacctgtag gtttggcaag ctagctgcag taacgccatt ttgcaaggca
     2341 tggaaaaata ccaaaccaag aatagagaag ttcagatcaa gggcgggtac atgaaaatag
     2401 ctaacgttgg gccaaacagg atatctgcgg tgagcagttt cggccccggc ccggggccaa
     2461 gaacagatgg tcaccgcagt ttcggccccg gcccgaggcc aagaacagat ggtccccaga
     2521 tatggcccaa ccctcagcag tttcttaaga cccatcagat gtttccaggc tcccccaagg
     2581 acctgaaatg accctgcgcc ttatttgaat taaccaatca gcctgcttct cgcttctgtt
     2641 cgcgcgcttc tgcttcccga gctctataaa agagctcaca acccctcact cggcgcgcca
     2701 gtcctccgac agactgagtc gcccgggggg gatctggagc tctcgagaat tctcacgcgt
     2761 caagtggagc aaggcaggtg gacagtggat ccccatggct ccaaagaaga agcgtaaggt
     2821 acggtaccca gctttcttgt acaaagtggc caccggatcc atggcctcaa acgattatac
     2881 ccaacaagca acccaaagct atggggccta ccccacccag cccgggcagg gctattccca
     2941 gcagagcagt cagccctacg gacagcagag ttacagtggt tatagccagt ccacggacac
     3001 ttcaggctat ggccagagca gctattcttc ttatggccag agccagaaca caggctatgg
     3061 aactcagtca actccccagg gatatggctc gactggcggc tatggcagta gccagagctc
     3121 ccaatcgtct tacgggcagc agtcctccta tcctggctat ggccagcagc cagctcccag
     3181 cagcacctcg ggaagttacg gtagcagttc tcagagcagc agctatgggc agccccagag
     3241 tgggagctac agccagcagc ctagctatgg tggacagcag caaagctatg gacagcagca
     3301 aagctataat ccccctcagg gctatggaca gcagaaccag tacaacagca gcagtggtgg
     3361 tggaggtgga ggtggaggtg gaggtaacta tggccaagat caatcctcca tgagtagtgg
     3421 tggtggcagt ggtggcggtt atggcaatca agaccagagt ggtggaggtg gcagcggtgg
     3481 ctatggacag caggaccgtg gaggtggagg aatggtgagc aagggcgagg agctgttcac
     3541 cggggtggtg cccatcctgg tcgagctgga cggcgacgta aacggccaca agttcagcgt
     3601 gtccggcgag ggcgagggcg atgccaccta cggcaagctg accctgaagt tcatctgcac
     3661 caccggcaag ctgcccgtgc cctggcccac cctcgtgacc accttcggct acggcctgat
     3721 gtgcttcgcc cgctaccccg accacatgaa gcagcacgac ttcttcaagt ccgccatgcc
     3781 cgaaggctac gtccaggagc gcaccatctt cttcaaggac gacggcaact acaagacccg
     3841 cgccgaggtg aagttcgagg gcgacaccct ggtgaaccgc atcgagctga agggcatcga
     3901 cttcaaggag gacggcaaca tcctggggca caagctggag tacaactaca acagccacaa
     3961 cgtctatatc atggccgaca agcagaagaa cggcatcaag gtgaacttca agatccgcca
     4021 caacatcgag gacggcagcg tgcagctcgc cgaccactac cagcagaaca cccccatcgg
     4081 cgacggcccc gtgctgctgc ccgacaacca ctacctgagc taccagtccg ccctgagcaa
     4141 agaccccaac gagaagcgcg atcacatggt cctgctggag ttcgtgaccg ccgccgggat
     4201 cactctcggc atggacgagc tgtacaaggc ggccacgcgt atgagcaatt cagttttgtc
     4261 cacttcttcc ccaggtaata tccaaactcg gcaggcgatt ccggcccaac tcctctattc
     4321 cctcgtcaac caaaaagcca gcggcaaact tgtggtacaa aacccctttg acgaatttgt
     4381 ttattggcaa gtttatttgg gggatggtcg tattcacttg gccaacagcg ccaatggttg
     4441 ttccgaaaga cttaattatc tgattggtag cacccttcgt cagaagggca ttgaactgcc
     4501 atcaagaatt gctgacgatt atatttttct ttccgggctc tggaaaaggg atgttttttc
     4561 ctttcagcaa acccgctcga ttctgaccca agctagccaa gaagccttag ttcaggtact
     4621 ttccttacct aaaacatcct atcgttttaa tcaagaaaaa gaactgagcg aactattttt
     4681 gaatttagag ttagaaaaaa ctattacccc catcaaacat aaaattcgct attggtggga
     4741 attgaggtcc tatattaact cgcctttcca gcgaccccta gtggaaaact ggcttagtct
     4801 gcgccaaaat ttggtgaaaa ataacctcca tggccaacat tggctaaaac gcttccacac
     4861 ttgcttggaa aatctaaatt gcttatatga aattgccggg catttggaaa tcagtacttt
     4921 ggagttggca ttaatgctac agcccatggt caagactggc gaaatcaaga tgctgcctta
     4981 tcaagaaatt caaacggatg accgcccttt ggtggcttat gttgaccata gggaagtcca
     5041 gcagcgcatg atgacctatt ctttggaaaa agaaggctat cgagccttag tactggatga
     5101 tccctttcgg gctttaacgg tgttgttgaa ttctcccccc aatctgatta tcataaatac
     5161 tgacttgccg gacatgaacg gttatcaact ctgttcgttt tgccgtaagt ccaatcaact
     5221 taaagatgtg ccgatcctac tattggggaa aggtgacaat ttgatcagcg gtattcgtat
     5281 caaactttgt caagcttctg ggtacttaag tgagcctttt gttccagaag aattactcga
     5341 tcgggtggca aaactccttc ccaccaataa aaccaactcc tgagcggccg caactcccac
     5401 ctgcaacatg cgtgactgac tgaggccgcg actctagagt cgacctgcag gcatgcaagc
     5461 ttgatatcaa gcttatcgat aatcaacctc tggattacaa aatttgtgaa agattgactg
     5521 gtattcttaa ctatgttgct ccttttacgc tatgtggata cgctgcttta atgcctttgt
     5581 atcatgctat tgcttcccgt atggctttca ttttctcctc cttgtataaa tcctggttgc
     5641 tgtctcttta tgaggagttg tggcccgttg tcaggcaacg tggcgtggtg tgcactgtgt
     5701 ttgctgacgc aacccccact ggttggggca ttgccaccac ctgtcagctc ctttccggga
     5761 ctttcgcttt ccccctccct attgccacgg cggaactcat cgccgcctgc cttgcccgct
     5821 gctggacagg ggctcggctg ttgggcactg acaattccgt ggtgttgtcg gggaaatcat
     5881 cgtcctttcc ttggctgctc gcctgtgttg ccacctggat tctgcgcggg acgtccttct
     5941 gctacgtccc ttcggccctc aatccagcgg accttccttc ccgcggcctg ctgccggctc
     6001 tgcggcctct tccgcgtctt cgccttcgcc ctcagacgag tcggatctcc ctttgggccg
     6061 cctccccgca tcgataccgt cgacctcgag ggaattaatt cgagctcggt acctttaaga
     6121 ccaatgactt acaaggcagc tgtagatctt agccactttt taaaagaaaa ggggggactg
     6181 gaagggctaa ttcactccca acgaagacaa gatctgcttt ttgcttgtac tgggtctctc
     6241 tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag
     6301 cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct
     6361 ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag cagcatctag
     6421 aattaattcc gtgtattcta tagtgtcacc taaatcgtat gtgtatgata cataaggtta
     6481 tgtattaatt gtagccgcgt tctaacgaca atatgtacaa gcctaattgt gtagcatctg
     6541 gcttactgaa gcagacccta tcatctctct cgtaaactgc cgtcagagtc ggtttggttg
     6601 gacgaacctt ctgagtttct ggtaacgccg tcccgcaccc ggaaatggtc agcgaaccaa
     6661 tcagcagggt catcgctagc cagatcctct acgccggacg catcgtggcc ggcatcaccg
     6721 gcgccacagg tgcggttgct ggcgcctata tcgccgacat caccgatggg gaagatcggg
     6781 ctcgccactt cgggctcatg agcgcttgtt tcggcgtggg tatggtggca ggccccgtgg
     6841 ccgggggact gttgggcgcc atctccttgc atgcaccatt ccttgcggcg gcggtgctca
     6901 acggcctcaa cctactactg ggctgcttcc taatgcagga gtcgcataag ggagagcgtc
     6961 gaatggtgca ctctcagtac aatctgctct gatgccgcat agttaagcca gccccgacac
     7021 ccgccaacac ccgctgacgc gccctgacgg gcttgtctgc tcccggcatc cgcttacaga
     7081 caagctgtga ccgtctccgg gagctgcatg tgtcagaggt tttcaccgtc atcaccgaaa
     7141 cgcgcgagac gaaagggcct cgtgatacgc ctatttttat aggttaatgt catgataata
     7201 atggtttctt agacgtcagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt
     7261 ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg
     7321 cttcaataat attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt
     7381 cccttttttg cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta
     7441 aaagatgctg aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc
     7501 ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa
     7561 gttctgctat gtggcgcggt attatcccgt attgacgccg ggcaagagca actcggtcgc
     7621 cgcatacact attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt
     7681 acggatggca tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact
     7741 gcggccaact tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac
     7801 aacatggggg atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata
     7861 ccaaacgacg agcgtgacac cacgatgcct gtagcaatgg caacaacgtt gcgcaaacta
     7921 ttaactggcg aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg
     7981 gataaagttg caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat
     8041 aaatctggag ccggtgagcg tgggtctcgc ggtatcattg cagcactggg gccagatggt
     8101 aagccctccc gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga
     8161 aatagacaga tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa
     8221 gtttactcat atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag
     8281 gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac
     8341 tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc
     8401 gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat
     8461 caagagctac caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat
     8521 actgttcttc tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct
     8581 acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt
     8641 cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg
     8701 gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta
     8761 cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg
     8821 gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg
     8881 tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc
     8941 tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg
     9001 gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat
     9061 aaccgtatta ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc
     9121 agcgagtcag tgagcgagga agcggaagag cgcccaatac gcaaaccgcc tctccccgcg
     9181 cgttggccga ttcattaatg cagctgtgga atgtgtgtca gttagggtgt ggaaagtccc
     9241 caggctcccc agcaggcaga agtatgcaaa gcatgcatct caattagtca gcaaccaggt
     9301 gtggaaagtc cccaggctcc ccagcaggca gaagtatgca aagcatgcat ctcaattagt
     9361 cagcaaccat agtcccgccc ctaactccgc ccatcccgcc cctaactccg cccagttccg
     9421 cccattctcc gccccatggc tgactaattt tttttattta tgcagaggcc gaggccgcct
     9481 cggcctctga gctattccag aagtagtgag gaggcttttt tggaggccta ggcttttgca
     9541 aaaagcttgg acacaagaca ggcttgcgag atatgtttga gaataccact ttatcccgcg
     9601 tcagggagag gcagtgcgta aaaagacgcg gactcatgtg aaatactggt ttttagtgcg
     9661 ccagatctct ataatctcgc gcaacctatt ttcccctcga acacttttta agccgtagat
     9721 aaacaggctg ggacacttca catgagcgaa aaatacatcg tcacctggga catgttgcag
     9781 atccatgcac gtaaactcgc aagccgactg atgccttctg aacaatggaa aggcattatt
     9841 gccgtaagcc gtggcggtct gtaccgggtg cgttactggc gcgtgaactg ggtattcgtc
     9901 atgtcgatac cgtttgtatt tccagctacg atcacgacaa ccagcgcgag cttaaagtgc
     9961 tgaaacgcgc agaaggcgat ggcgaaggct tcatcgttat tgatgacctg gtggataccg
    10021 gtggtactgc ggttgcgatt cgtgaaatgt atccaaaagc gcactttgtc accatcttcg
    10081 caaaaccggc tggtcgtccg ctggttgatg actatgttgt tgatatcccg caagatacct
    10141 ggattgaaca gccgtgggat atgggcgtcg tattcgtccc gccaatctcc ggtcgctaat
    10201 cttttcaacg cctggcactg ccgggcgttg ttctttttaa cttcaggcgg gttacaatag
    10261 tttccagtaa gtattctgga ggctgcatcc atgacacagg caaacctgag cgaaaccctg
    10321 ttcaaacccc gctttaaaca tcctgaaacc tcgacgctag tccgccgctt taatcacggc
    10381 gcacaaccgc ctgtgcagtc ggcccttgat ggtaaaacca tccctcactg gtatcgcatg
    10441 attaaccgtc tgatgtggat ctggcgcggc attgacccac gcgaaatcct cgacgtccag
    10501 gcacgtattg tgatgagcga tgccgaacgt accgacgatg atttatacga tacggtgatt
    10561 ggctaccgtg gcggcaactg gatttatgag tgggccccgg atctttgtga aggaacctta
    10621 cttctgtggt gtgacataat tggacaaact acctacagag atttaaagct ctaaggtaaa
    10681 tataaaattt ttaagtgtat aatgtgttaa actactgatt ctaattgttt gtgtatttta
    10741 gattccaacc tatggaactg atgaatggga gcagtggtgg aatgccttta atgaggaaaa
    10801 cctgttttgc tcagaagaaa tgccatctag tgatgatgag gctactgctg actctcaaca
    10861 ttctactcct ccaaaaaaga agagaaaggt agaagacccc aaggactttc cttcagaatt
    10921 gctaagtttt ttgagtcatg ctgtgtttag taatagaact cttgcttgct ttgctattta
    10981 caccacaaag gaaaaagctg cactgctata caagaaaatt atggaaaaat attctgtaac
    11041 ctttataagt aggcataaca gttataatca taacatactg ttttttctta ctccacacag
    11101 gcatagagtg tctgctatta ataactatgc tcaaaaattg tgtaccttta gctttttaat
    11161 ttgtaaaggg gttaataagg aatatttgat gtatagtgcc ttgactagag atcataatca
    11221 gccataccac atttgtagag gttttacttg ctttaaaaaa cctcccacac ctccccctga
    11281 acctgaaaca taaaatgaat gcaattgttg ttgttaactt gtttattgca gcttataatg
    11341 gttacaaata aagcaatagc atcacaaatt tcacaaataa agcatttttt tcactgcatt
    11401 ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca tgtctggatc aactggataa
    11461 ctcaagctaa ccaaaatcat cccaaacttc ccaccccata ccctattacc actgccaatt
    11521 acctgtggtt tcatttactc taaacctgtg attcctctga attattttca ttttaaagaa
    11581 attgtatttg ttaaatatgt actacaaact tagtagt
//
