LOCUS       sequence-212670-        8945 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses HM13.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8945)
  AUTHORS   Galligan JT, Martinez-Noel G, Arndt V, Hayes S, Chittenden TW, 
            Harper JW, Howley PM
  TITLE     Proteomic analysis and identification of cellular interactors of the
            giant ubiquitin ligase HERC2.
  JOURNAL   J Proteome Res. 2015 Feb 6;14(2):953-66. doi: 10.1021/pr501005v. 
            Epub 2014 Dec 15.
  PUBMED    25476789
REFERENCE   2  (bases 1 to 8945)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1021/pr501005v";
            journalName: "J Proteome Res"; date: "2015-02-6- 6"; volume: "14"; 
            issue: "2"; pages: "953-66"
COMMENT     Sequence Label: p7129 pHAGE-P-CMVt-N-HA-GAW-HM13
FEATURES             Location/Qualifiers
     source          1..8945
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      15..603
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     504..523
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     757..774
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    1014..1035
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(1050..1055,1056..1073,1074..1080)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1088..1104
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     1093..1115
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     1112..1128
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     1112..1128
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     1178..1197
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             1389..2022
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    2069..2194
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    2691..2924
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             3109..3153
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    3446..3563
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     enhancer        3615..3918
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        3919..4122
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     4073..4093
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     protein_bind    4124..4142
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    4145..4163
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     4173..4197
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     regulatory      4284..4293
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             4296..4322
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     protein_bind    4338..4362
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(5494..5518)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     promoter        5537..6036
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(5572..5595)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     5969..5988
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             6057..6656
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(6057..6076)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     6553..6573
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    6723..7311
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(6776..6796)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(7194..7205)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             7386..7619
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     primer_bind     complement(7738..7756)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        7824..7928
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7929..7997,7998..8789)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8147..8166)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa
       61 aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg
      121 aaggtaactg gcttcagcag agcgcagata ccaaatactg ttcttctagt gtagccgtag
      181 ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg
      241 ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga
      301 tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc
      361 ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc
      421 acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga
      481 gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt
      541 cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg
      601 aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac
      661 atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga
      721 gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg
      781 gaagagcgcc caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc
      841 aagctcatgg ctgactaatt ttttttattt atgcagaggc cgaggccgcc tcggcctctg
      901 agctattcca gaagtagtga ggaggctttt ttggaggcct aggcttttgc aaaaagctcc
      961 ccgtggcacg acaggtttcc cgactggaaa gcgggcagtg agcgcaacgc aattaatgtg
     1021 agttagctca ctcattaggc accccaggct ttacacttta tgcttccggc tcgtatgttg
     1081 tgtggaattg tgagcggata acaatttcac acaggaaaca gctatgacat gattacgaat
     1141 ttcacaaata aagcattttt ttcactgcat tctagttgtg gtttgtccaa actcatcaat
     1201 gtatcttatc atgtctggat caactggata actcaagcta accaaaatca tcccaaactt
     1261 cccaccccat accctattac cactgccaat tacctgtggt ttcatttact ctaaacctgt
     1321 gattcctctg aattattttc attttaaaga aattgtattt gttaaatatg tactacaaac
     1381 ttagtagttg gaagggctaa ttcactccca aagaagacaa gatatccttg atctgtggat
     1441 ctaccacaca caaggctact tccctgatta gcagaactac acaccagggc caggggtcag
     1501 atatccactg acctttggat ggtgctacaa gctagtacca gttgagccag ataaggtaga
     1561 agaggccaat aaaggagaga acaccagctt gttacaccct gtgagcctgc atgggatgga
     1621 tgacccggag agagaagtgt tagagtggag gtttgacagc cgcctagcat ttcatcacgt
     1681 ggcccgagag ctgcatccgg agtacttcaa gaactgctga tatcgagctt gctacaaggg
     1741 actttccgct ggggactttc cagggaggcg tggcctgggc gggactgggg agtggcgagc
     1801 cctcagatcc tgcatataag cagctgcttt ttgcctgtac tgggtctctc tggttagacc
     1861 agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag cctcaataaa
     1921 gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct ggtaactaga
     1981 gatccctcag acccttttag tcagtgtgga aaatctctag cagtggcgcc cgaacaggga
     2041 cttgaaagcg aaagggaaac cagaggagct ctctcgacgc aggactcggc ttgctgaagc
     2101 gcgcacggca agaggcgagg ggcggcgact ggtgagtacg ccaaaaattt tgactagcgg
     2161 aggctagaag gagagagatg ggtgcgagag cgtcagtatt aagcggggga gaattagatc
     2221 gcgatgggaa aaaattcggt taaggccagg gggaaagaaa aaatataaat taaaacatat
     2281 agtatgggca agcagggagc tagaacgatt cgcagttaat cctggcctgt tagaaacatc
     2341 agaaggctgt agacaaatac tgggacagct acaaccatcc cttcagacag gatcagaaga
     2401 acttagatca ttatataata cagtagcaac cctctattgt gtgcatcaaa ggatagagat
     2461 aaaagacacc aaggaagctt tagacaagat agaggaagag caaaacaaaa gtaagaccac
     2521 cgcacagcaa gcggccggcc gctgatcttc agacctggag gaggagatat gagggacaat
     2581 tggagaagtg aattatataa atataaagta gtaaaaattg aaccattagg agtagcaccc
     2641 accaaggcaa agagaagagt ggtgcagaga gaaaaaagag cagtgggaat aggagctttg
     2701 ttccttgggt tcttgggagc agcaggaagc actatgggcg cagcgtcaat gacgctgacg
     2761 gtacaggcca gacaattatt gtctggtata gtgcagcagc agaacaattt gctgagggct
     2821 attgaggcgc aacagcatct gttgcaactc acagtctggg gcatcaagca gctccaggca
     2881 agaatcctgg ctgtggaaag atacctaaag gatcaacagc tcctggggat ttggggttgc
     2941 tctggaaaac tcatttgcac cactgctgtg ccttggaatg ctagttggag taataaatct
     3001 ctggaacaga tttggaatca cacgacctgg atggagtggg acagagaaat taacaattac
     3061 acaagcttaa tacactcctt aattgaagaa tcgcaaaacc agcaagaaaa gaatgaacaa
     3121 gaattattgg aattagataa atgggcaagt ttgtggaatt ggtttaacat aacaaattgg
     3181 ctgtggtata taaaattatt cataatgata gtaggaggct tggtaggttt aagaatagtt
     3241 tttgctgtac tttctatagt gaatagagtt aggcagggat attcaccatt atcgtttcag
     3301 acccacctcc caaccccgag gggacccgac aggcccgaag gaatagaaga agaaggtgga
     3361 gagagagaca gagacagatc cattcgatta gtgaacggat ctcgacggta tcgccgaatt
     3421 cacaaatggc agtattcatc cacaatttta aaagaaaagg ggggattggg gggtacagtg
     3481 caggggaaag aatagtagac ataatagcaa cagacataca aactaaagaa ttacaaaaac
     3541 aaattacaaa aattcaaaat tttcgggttt attacaggga cagcagagat ccagtttgga
     3601 ctagtggagt tccgcgttac ataacttacg gtaaatggcc cgcctggctg accgcccaac
     3661 gacccccgcc cattgacgtc aataatgacg tatgttccca tagtaacgcc aatagggact
     3721 ttccattgac gtcaatgggt ggagtattta cggtaaactg cccacttggc agtacatcaa
     3781 gtgtatcata tgccaagtac gccccctatt gacgtcaatg acggtaaatg gcccgcctgg
     3841 cattatgccc agtacatgac cttatgggac tttcctactt ggcagtacat ctacgtatta
     3901 gtcatcgcta ttaccatggt gatgcggttt tggcagtaca tcaatgggcg tggatagcgg
     3961 tttgactcac ggggatttcc aagtctccac cccattgacg tcaatgggag tttgttttgg
     4021 caccaaaatc aacgggactt tccaaaatgt cgtaacaact ccgccccatt gacgcaaatg
     4081 ggcggtaggc gtgtacggtg ggaggtctat ataagcagag ctctccctat cagtgataga
     4141 gatctcccta tcagtgatag agatcgtcga cgagctcgtt tagtgaaccg tcagatcgcc
     4201 tggagacgcc atccacgctg ttttgacctc catagaagac accgggaccg atccagcctc
     4261 cggactctag cgtttaaacg ctagccacca tggcgtaccc atacgatgtt ccagattacg
     4321 ctgctcgagg ttatcaaaca agtttgtaca aaaaagttgg catggactcg gccctcagcg
     4381 atccgcataa cggcagtgcc gaggcaggcg gccccaccaa cagcactacg cggccgcctt
     4441 ccacgcccga gggcatcgcg ctggcctacg gcagcctcct gctcatggcg ctgctgccca
     4501 tcttcttcgg cgccctgcgc tccgtacgct gcgcccgcgg caagaatgct tcagacatgc
     4561 ctgaaacaat caccagccgg gatgccgccc gcttccccat catcgccagc tgcacactct
     4621 tggggctcta cctctttttc aaaatattct cccaggagta catcaacctc ctgctgtcca
     4681 tgtatttctt cgtgctggga atcctggccc tgtcccacac catcagcccc ttcatgaata
     4741 agttttttcc agccagcttt ccaaatcgac agtaccagct gctcttcaca cagggttctg
     4801 gggaaaacaa ggaagagatc atcaattatg aatttgacac caaggacctg gtgtgcctgg
     4861 gcctgagcag catcgttggc gtctggtacc tgctgaggaa gcactggatt gccaacaacc
     4921 tttttggcct ggccttctcc cttaatggag tagagctcct gcacctcaac aatgtcagca
     4981 ctggctgcat cctgctgggc ggactcttca tctacgatgt cttctgggta tttggcacca
     5041 atgtgatggt gacagtggcc aagtccttcg aggcaccaat aaaattggtg tttccccagg
     5101 atctgctgga gaaaggcctc gaagcaaaca actttgccat gctgggactt ggagatgtcg
     5161 tcattccagg gatcttcatt gccttgctgc tgcgctttga catcagcttg aagaagaata
     5221 cccacaccta cttctacacc agctttgcag cctacatctt cggcctgggc cttaccatct
     5281 tcatcatgca catcttcaag catgctcagc ctgccctcct atacctggtc cccgcctgca
     5341 tcggttttcc tgtcctggtg gcgctggcca agggagaagt gacagagatg ttcagttatg
     5401 aggagtcaaa tcctaaggat ccagcggcag tgacagaatc caaagaggga acagaggcat
     5461 cagcatcgaa ggggctggag aagaaagaga aatgctcaac tttcttgtac aaagtggttc
     5521 gataacgaat tctaccgggt aggggaggcg cttttcccaa ggcagtctgg agcatgcgct
     5581 ttagcagccc cgctgggcac ttggcgctac acaagtggcc tctggcctcg cacacattcc
     5641 acatccaccg gtaggcgcca accggctccg ttctttggtg gccccttcgc gccaccttct
     5701 actcctcccc tagtcaggaa gttccccccc gccccgcagc tcgcgtcgtg caggacgtga
     5761 caaatggaag tagcacgtct cactagtctc gtgcagatgg acagcaccgc tgagcaatgg
     5821 aagcgggtag gcctttgggg cagcggccaa tagcagcttt gctccttcgc tttctgggct
     5881 cagaggctgg gaaggggtgg gtccgggggc gggctcaggg gcgggctcag gggcggggcg
     5941 ggcgcccgaa ggtcctccgg aggcccggca ttctgcacgc ttcaaaagcg cacgtctgcc
     6001 gcgctgttct cctcttcctc atctccgggc ctttcgacct gcagcccaag cttaccatga
     6061 ccgagtacaa gcccacggtg cgcctcgcca cccgcgacga cgtccccagg gccgtacgca
     6121 ccctcgccgc cgcgttcgcc gactaccccg ccacgcgcca caccgtcgat ccggaccgcc
     6181 acatcgagcg ggtcaccgag ctgcaagaac tcttcctcac gcgcgtcggg ctcgacatcg
     6241 gcaaggtgtg ggtcgcggac gacggcgccg cggtggcggt ctggaccacg ccggagagcg
     6301 tcgaagcggg ggcggtgttc gccgagatcg gcccgcgcat ggccgagttg agcggttccc
     6361 ggctggccgc gcagcaacag atggaaggcc tcctggcgcc gcaccggccc aaggagcccg
     6421 cgtggttcct ggccaccgtc ggcgtctcgc ccgaccacca gggcaagggt ctgggcagcg
     6481 ccgtcgtgct ccccggagtg gaggcggccg agcgcgccgg ggtgcccgcc ttcctggaga
     6541 cctccgcgcc ccgcaacctc cccttctacg agcggctcgg cttcaccgtc accgccgacg
     6601 tcgaggtgcc cgaaggaccg cgcacctggt gcatgacccg caagcccggt gcctgacgcc
     6661 cgccccacga cccgcagcgc ccgaccgaaa ggagcgcacg accccatgca tcgatagatc
     6721 ctaatcaacc tctggattac aaaatttgtg aaagattgac tggtattctt aactatgttg
     6781 ctccttttac gctatgtgga tacgctgctt taatgccttt gtatcatgct attgcttccc
     6841 gtatggcttt cattttctcc tccttgtata aatcctggtt gctgtctctt tatgaggagt
     6901 tgtggcccgt tgtcaggcaa cgtggcgtgg tgtgcactgt gtttgctgac gcaaccccca
     6961 ctggttgggg cattgccacc acctgtcagc tcctttccgg gactttcgct ttccccctcc
     7021 ctattgccac ggcggaactc atcgccgcct gccttgcccg ctgctggaca ggggctcggc
     7081 tgttgggcac tgacaattcc gtggtgttgt cggggaaatc atcgtccttt ccttggctgc
     7141 tcgcctgtgt tgccacctgg attctgcgcg ggacgtcctt ctgctacgtc ccttcggccc
     7201 tcaatccagc ggaccttcct tcccgcggcc tgctgccggc tctgcggcct cttccgcgtc
     7261 ttcgccttcg ccctcagacg agtcggatct ccctttgggc cgcctccccg cctgagatcc
     7321 tttaagacca atgacttaca aggcagctgt agatcttagc cactttttaa aagaaaaggg
     7381 gggactggaa gggctaattc actcccaacg aagacaagat ctgctttttg cttgtactgg
     7441 gtctctctgg ttagaccaga tctgagcctg ggagctctct ggctaactag ggaacccact
     7501 gcttaagcct caataaagct tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg
     7561 tgactctggt aactagagat ccctcagacc cttttagtca gtgtggaaaa tctctagcag
     7621 tagtagttca tgtcatctta ttattcagta tttataactt gcaaagaaat gaatatcaga
     7681 gagtgagagg cccgggttaa ttaaggaaag ggctagatca ttcttgaaga cgaaagggcc
     7741 tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag
     7801 gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt
     7861 caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa
     7921 ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt
     7981 gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt
     8041 tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt
     8101 ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg
     8161 tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga
     8221 atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa
     8281 gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga
     8341 caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa
     8401 ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca
     8461 ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta
     8521 ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac
     8581 ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc
     8641 gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag
     8701 ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga
     8761 taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt
     8821 agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata
     8881 atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag
     8941 aaaag
//
