LOCUS       sequence-212671-        4656 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Parental vector for making S. rosetta expression vectors.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4656)
  AUTHORS   Booth DS, Szmidt-Middleton H, King N
  TITLE     Transfection of choanoflagellates illuminates their cell biology and
            the ancestry of animal septins.
  JOURNAL   Mol Biol Cell. 2018 Dec 1;29(25):3026-3038. doi: 
            10.1091/mbc.E18-08-0514. Epub 2018 Oct 3.
  PUBMED    30281390
REFERENCE   2  (bases 1 to 4656)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1091/mbc.E18-08-0514"; journalName: "Mol Biol Cell"; date: 
            "2018-12-1- 1"; volume: "29"; issue: "25"; pages: "3026-3038"
COMMENT     Sequence Label: NK586
FEATURES             Location/Qualifiers
     source          1..4656
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(16..34)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        102..206
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(207..275,276..1067)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(425..444)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1238..1826
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1727..1746
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1980..1997
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    2114..2135
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(2150..2155,2156..2173,2174..2180)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2188..2204
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     2193..2215
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     2212..2228
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     2212..2228
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(4268..4285)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(4268..4284)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4277..4299)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(4493..4512)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     4612..4634
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
ORIGIN
        1 gcgcgagacg aaagggcctc gtgatacgcc tatttttata ggttaatgtc atgataataa
       61 tggtttctta gacgtcaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt
      121 tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc
      181 ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc
      241 ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa
      301 aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg
      361 gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag
      421 ttctgctatg tggcgcggta ttatcccgta ttgacgccgg gcaagagcaa ctcggtcgcc
      481 gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta
      541 cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg
      601 cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca
      661 acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac
      721 caaacgacga gcgtgacacc acgatgcctg tagcaatggc aacaacgttg cgcaaactat
      781 taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg
      841 ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata
      901 aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta
      961 agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa
     1021 atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag
     1081 tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg
     1141 tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact
     1201 gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg
     1261 taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc
     1321 aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata
     1381 ctgttcttct agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta
     1441 catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc
     1501 ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg
     1561 ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac
     1621 agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg
     1681 taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt
     1741 atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct
     1801 cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg
     1861 ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata
     1921 accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca
     1981 gcgagtcagt gagcgaggaa gcggaagagc gcccaatacg caaaccgcct ctccccgcgc
     2041 gttggccgat tcattaatgc agctggcacg acaggtttcc cgactggaaa gcgggcagtg
     2101 agcgcaacgc aattaatgtg agttagctca ctcattaggc accccaggct ttacacttta
     2161 tgcttccggc tcgtatgttg tgtggaattg tgagcggata acaatttcac acaggaaaca
     2221 gctatgacca tgattacgcc aagcttgtaa caaactgccc tgcacctcgt ctgccacctc
     2281 ggttgccacc ttgtcctcgt tccccaccac cttgtcctcg tccgctgcca gcatcgcgat
     2341 cagcgtgctt gcgcttggcg ctgctgctgc cggacttccc gttatcggcc atactttttg
     2401 tggtgtttcc aggcgagatg cgctgttggc aaagcaaaat ggcaaaacgg cctttgccac
     2461 acgccatcct cgcctcacct cgacgtgcgg ctttcctaaa caggaaacct gacgaaacga
     2521 attgagcgca ttggcacacg tgtgctgtgc gctttgtgtc gctgcatgct atccaccacg
     2581 caccacccaa tcagtccaac tgtgacgtgt cacgccatgg cgaaaaagcg gcgagagcgc
     2641 caaagcaaac gccaaacaaa aaccaaagaa gagcaaacgc agcgaactga tgtgagacaa
     2701 gggggcggtg caatgcgtca ctgaacgtgc aaaagccaaa aacaaccaag aagcagctgc
     2761 acaacacaac aacaacaaca acaacaacaa caccttgttg tcgcagctct acgtttggca
     2821 ttttcctgtt gttctctctt gcgatcagtc cgtgtctctt ttctccccct tttcccagac
     2881 cacaaccaca aaacaaccag ccatgggtga cgaggatgtt gctgctctcg ttgttgacaa
     2941 cggctccggc atgtgcaagg ccggcttcgc cggtgacgac gctccccgcg ccgtcttccc
     3001 ctccattgtt ggccgtcccc gccaccaggg cgtgatggtc ggcatgggcc agaaggactc
     3061 gtacgtcggc gacgaggccc agagcaagcg tggtatcctc acgctcaagt accccattga
     3121 gcacggtatt gtgaccaact gggacgacat ggagaagatc tggcaccaca ccttctacaa
     3181 cgagctccgc gttgcgcccg aggagcaccc cgtcctcctc accgaggccc cgctcaaccc
     3241 caaggccaac cgtgagaaga tgacccagat catgttcgag acgttcaaca cccccgccat
     3301 gtacgtcgcc atccaggccg tgctctccct gtacgcctct ggccgtacca cgggtatcgt
     3361 cctcgactct ggcgatggtg tcacccacac cgtccccatc tacgagggtt acgcccttcc
     3421 ccacgccatc ctgcgtctcg accttgccgg ccgcgatctc accgactaca tgatgaagat
     3481 cctcaccgag cgcggttact ccttcaccac caccgccgag cgcgagattg tgcgtgacat
     3541 caaggagaag ctctcctacg tcgccctcga ctttgagcag gagatggcca ccgccgcctc
     3601 ctcctccagc ctggagaaga gctacgagct ccccgatggc caggtcatca ccattggcaa
     3661 cgagcgcttc cgctgccccg aggccctctt ccagccatcc ttcctcggca tggaggccac
     3721 cggcatccac gagcacacct acaactcgat catgaagtgc gacgtcgaca tccgtaagga
     3781 cctctacgcc aacaccgtcc tctctggcgg taccaccatg ttccccggca ttgccgaccg
     3841 catgcagaag gagatcaccg ccctcgcccc atccaccatg aagatcaaga tcattgctcc
     3901 tcccgagcgc aagtactctg tctggattgg cggctccatc ctcgcctccc tctccacctt
     3961 ccagcagatg tggatctcca aggccgagta cgacgagtct ggcccctcga ttgtccaccg
     4021 caagtgcttc taagcgcctc gttggtcatt gccaccttgt tgcctctttt gctgttgtcc
     4081 tcgtgtcgac gcgtgccacg tgcgcttgtt gtgagttgat tctgcttctt gtgcacgtca
     4141 tgccgagccc ttgggccccg caataaaccc actatgccag tgtaactatc cttcccccca
     4201 atgtataact gtgacgatca atgagcggtg cccgtctaga ggatccccgg gtaccgagct
     4261 cgaattcact ggccgtcgtt ttacaacgtc gtgactggga aaaccctggc gttacccaac
     4321 ttaatcgcct tgcagcacat ccccctttcg ccagctggcg taatagcgaa gaggcccgca
     4381 ccgatcgccc ttcccaacag ttgcgcagcc tgaatggcga atggcgcctg atgcggtatt
     4441 ttctccttac gcatctgtgc ggtatttcac accgcatatg gtgcactctc agtacaatct
     4501 gctctgatgc cgcatagtta agccagcccc gacacccgcc aacacccgct gacgcgccct
     4561 gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct
     4621 gcatgtgtca gaggttttca ccgtcatcac cgaaac
//
