LOCUS       sequence-212675-        8822 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral  expression of shRNA targeting PBRM.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8822)
  AUTHORS   Gao W, Li W, Xiao T, Liu XS, Kaelin WG Jr
  TITLE     Inactivation of the PBRM1 tumor suppressor gene amplifies the 
            HIF-response in VHL-/- clear cell renal carcinoma.
  JOURNAL   Proc Natl Acad Sci U S A. 2017 Jan 31;114(5):1027-1032. doi: 
            10.1073/pnas.1619726114. Epub 2017 Jan 12.
  PUBMED    28082722
REFERENCE   2  (bases 1 to 8822)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1619726114"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2017-01-31- 31"; volume: "114"; issue: "5"; pages: 
            "1027-1032"
COMMENT     Sequence Label: shPBRM1#32-Tet-pLKO-puro
FEATURES             Location/Qualifiers
     source          1..8822
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    80..101
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(116..121,122..139,140..146)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    154..170
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     159..181
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     178..194
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     178..194
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     213..233
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        215..233
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        261..487
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             488..668
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    715..840
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1333..1566
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1751..1795
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        2084..2178
                     /label=H1-2O2 promoter
                     /note="doxycycline-inducible variant of the human H1 RNA 
                     promoter (Henriksen et al., 2007)"
     primer_bind     2095..2114
                     /label=H1
                     /note="Human H1 promoter, forward primer"
     protein_bind    2132..2150
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2160..2178
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     misc_feature    2285..2402
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2451..2961
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     2880..2898
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     intron          2970..3542
                     /label=beta-globin intron
                     /note="intron from rabbit beta-globin gene"
     primer_bind     3550..3569
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     promoter        3597..3615
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             3626..4246
                     /codon_start=1
                     /gene="tetR from transposon Tn10"
                     /product="tetracycline repressor TetR"
                     /label=TetR
                     /note="TetR binds to the tetracycline operator tetO to 
                     inhibit transcription. This inhibition can be relieved by 
                     adding tetracycline or doxycycline."
                     /translation="MSRLDKSKVINSALELLNEVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALAIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEDQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDHQGAEPAFLFGLELIICGLEKQLKCESGS"
     primer_bind     complement(3699..3718)
                     /label=Tet-R
                     /note="Tetracycline resistance gene, reverse primer"
     misc_feature    4281..4854
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(4448..4465)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     4675..4694
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             4874..5473
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(4874..4893)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     5370..5390
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     LTR             5601..5834
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    5906..6027
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5943..5962)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5997..6016
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      6067..6202
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     promoter        complement(6223..6241)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(6251..6268)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(6251..6267)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6260..6282)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      6409..6864
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6496..6515)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6706..6727
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        6890..6994
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6995..7063,7064..7855)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7213..7232)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8026..8614
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8515..8534
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8768..8785
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 ccgcgcgttg gccgattcat taatgcagct ggcacgacag gtttcccgac tggaaagcgg
       61 gcagtgagcg caacgcaatt aatgtgagtt agctcactca ttaggcaccc caggctttac
      121 actttatgct tccggctcgt atgttgtgtg gaattgtgag cggataacaa tttcacacag
      181 gaaacagcta tgaccatgat tacgccaagc gcgcaattaa ccctcactaa agggaacaaa
      241 agctggagct gcaagcttaa tgtagtctta tgcaatactc ttgtagtctt gcaacatggt
      301 aacgatgagt tagcaacatg ccttacaagg agagaaaaag caccgtgcat gccgattggt
      361 ggaagtaagg tggtacgatc gtgccttatt aggaaggcaa cagacgggtc tgacatggat
      421 tggacgaacc actgaattgc cgcattgcag agatattgta tttaagtgcc tagctcgata
      481 cataaacggg tctctctggt tagaccagat ctgagcctgg gagctctctg gctaactagg
      541 gaacccactg cttaagcctc aataaagctt gccttgagtg cttcaagtag tgtgtgcccg
      601 tctgttgtgt gactctggta actagagatc cctcagaccc ttttagtcag tgtggaaaat
      661 ctctagcagt ggcgcccgaa cagggacttg aaagcgaaag ggaaaccaga ggagctctct
      721 cgacgcagga ctcggcttgc tgaagcgcgc acggcaagag gcgaggggcg gcgactggtg
      781 agtacgccaa aaattttgac tagcggaggc tagaaggaga gagatgggtg cgagagcgtc
      841 agtattaagc gggggagaat tagatcgcga tgggaaaaaa ttcggttaag gccaggggga
      901 aagaaaaaat ataaattaaa acatatagta tgggcaagca gggagctaga acgattcgca
      961 gttaatcctg gcctgttaga aacatcagaa ggctgtagac aaatactggg acagctacaa
     1021 ccatcccttc agacaggatc agaagaactt agatcattat ataatacagt agcaaccctc
     1081 tattgtgtgc atcaaaggat agagataaaa gacaccaagg aagctttaga caagatagag
     1141 gaagagcaaa acaaaagtaa gaccaccgca cagcaagcgg ccgctgatct tcagacctgg
     1201 aggaggagat atgagggaca attggagaag tgaattatat aaatataaag tagtaaaaat
     1261 tgaaccatta ggagtagcac ccaccaaggc aaagagaaga gtggtgcaga gagaaaaaag
     1321 agcagtggga ataggagctt tgttccttgg gttcttggga gcagcaggaa gcactatggg
     1381 cgcagcgtca atgacgctga cggtacaggc cagacaatta ttgtctggta tagtgcagca
     1441 gcagaacaat ttgctgaggg ctattgaggc gcaacagcat ctgttgcaac tcacagtctg
     1501 gggcatcaag cagctccagg caagaatcct ggctgtggaa agatacctaa aggatcaaca
     1561 gctcctgggg atttggggtt gctctggaaa actcatttgc accactgctg tgccttggaa
     1621 tgctagttgg agtaataaat ctctggaaca gatttggaat cacacgacct ggatggagtg
     1681 ggacagagaa attaacaatt acacaagctt aatacactcc ttaattgaag aatcgcaaaa
     1741 ccagcaagaa aagaatgaac aagaattatt ggaattagat aaatgggcaa gtttgtggaa
     1801 ttggtttaac ataacaaatt ggctgtggta tataaaatta ttcataatga tagtaggagg
     1861 cttggtaggt ttaagaatag tttttgctgt actttctata gtgaatagag ttaggcaggg
     1921 atattcacca ttatcgtttc agacccacct cccaaccccg aggggacccg acaggcccga
     1981 aggaatagaa gaagaaggtg gagagagaga cagagacaga tccattcgat tagtgaacgg
     2041 atctcgacgg tatcgatcac gagactagcc tcgagcggcc gcaatatttg catgtcgcta
     2101 tgtgttctgg gaaatcacca taaacgtgaa atccctatca gtgatagaga cttataagtt
     2161 ccctatcagt gatagagaca ccgggcactc agctatacca caaaactcga gttttgtggt
     2221 atagctgagt gctttttttt ttttaattct cgacctcgag acaaatggca gtattcatcc
     2281 acaattttaa aagaaaaggg gggattgggg ggtacagtgc aggggaaaga atagtagaca
     2341 taatagcaac agacatacaa actaaagaat tacaaaaaca aattacaaaa attcaaaatt
     2401 ttcgggttta ttacagggac agcagagatc cactttggcc gcggctcgag ggggttgggg
     2461 ttgcgccttt tccaaggcag ccctgggttt gcgcagggac gcggctgctc tgggcgtggt
     2521 tccgggaaac gcagcggcgc cgaccctggg tctcgcacat tcttcacgtc cgttcgcagc
     2581 gtcacccgga tcttcgccgc tacccttgtg ggccccccgg cgacgcttcc tgctccgccc
     2641 ctaagtcggg aaggttcctt gcggttcgcg gcgtgccgga cgtgacaaac ggaagccgca
     2701 cgtctcacta gtaccctcgc agacggacag cgccagggag caatggcagc gcgccgaccg
     2761 cgatgggctg tggccaatag cggctgctca gcagggcgcg ccgagagcag cggccgggaa
     2821 ggggcggtgc gggaggcggg gtgtggggcg gtagtgtggg ccctgttcct gcccgcgcgg
     2881 tgttccgcat tctgcaagcc tccggagcgc acgtcggcag tcggctccct cgttgaccga
     2941 atcaccgacc tctctcccca gggggatctg tgagtttggg gacccttgat tgttctttct
     3001 ttttcgctat tgtaaaattc atgttatatg gagggggcaa agttttcagg gtgttgttta
     3061 gaatgggaag atgtcccttg tatcaccatg gaccctcatg ataattttgt ttctttcact
     3121 ttctactctg ttgacaacca ttgtctcctc ttattttctt ttcattttct gtaacttttt
     3181 cgttaaactt tagcttgcat ttgtaacgaa tttttaaatt cacttttgtt tatttgtcag
     3241 attgtaagta ctttctctaa tcactttttt ttcaaggcaa tcagggtata ttatattgta
     3301 cttcagcaca gttttagaga acaattgtta taattaaatg ataaggtaga atatttctgc
     3361 atataaattc tggctggcgt ggaaatattc ttattggtag aaacaactac atcctggtca
     3421 tcatcctgcc tttctcttta tggttacaat gatatacact gtttgagatg aggataaaat
     3481 actctgagtc caaaccgggc ccctctgcta accatgttca tgccttcttc tttttcctac
     3541 agctcctggg caacgtgctg gttattgtgc tgtctcatca ttttggcaaa gaattgtaat
     3601 acgactcact atagggcgaa ttgatatgtc tagattagat aaaagtaaag tgattaacag
     3661 cgcattagag ctgcttaatg aggtcggaat cgaaggttta acaacccgta aactcgccca
     3721 gaagctaggt gtagagcagc ctacattgta ttggcatgta aaaaataagc gggctttgct
     3781 cgacgcctta gccattgaga tgttagatag gcaccatact cacttttgcc ctttagaagg
     3841 ggaaagctgg caagattttt tacgtaataa cgctaaaagt tttagatgtg ctttactaag
     3901 tcatcgcgat ggagcaaaag tacatttagg tacacggcct acagaaaaac agtatgaaac
     3961 tctcgaaaat caattagcct ttttatgcca acaaggtttt tcactagaga atgcattata
     4021 tgcactcagc gctgtggggc attttacttt aggttgcgta ttggaagatc aagagcatca
     4081 agtcgctaaa gaagaaaggg aaacacctac tactgatagt atgccgccat tattacgaca
     4141 agctatcgaa ttatttgatc accaaggtgc agagccagcc ttcttattcg gccttgaatt
     4201 gatcatatgc ggattagaaa aacaacttaa atgtgaaagt gggtccgcgt acagcggctc
     4261 ccgggagttc tagggatctg cccctctccc tccccccccc ctaacgttac tggccgaagc
     4321 cgcttggaat aaggccggtg tgcgtttgtc tatatgttat tttccaccat attgccgtct
     4381 tttggcaatg tgagggcccg gaaacctggc cctgtcttct tgacgagcat tcctaggggt
     4441 ctttcccctc tcgccaaagg aatgcaaggt ctgttgaatg tcgtgaagga agcagttcct
     4501 ctggaagctt cttgaagaca aacaacgtct gtagcgaccc tttgcaggca gcggaacccc
     4561 ccacctggcg acaggtgcct ctgcggccaa aagccacgtg tataagatac acctgcaaag
     4621 gcggcacaac cccagtgcca cgttgtgagt tggatagttg tggaaagagt caaatggctc
     4681 tcctcaagcg tattcaacaa ggggctgaag gatgcccaga aggtacccca ttgtatggga
     4741 tctgatctgg ggcctcggtg cacatgcttt acatgtgttt agtcgaggtt aaaaaaacgt
     4801 ctaggccccc cgaaccacgg ggacgtggtt ttcctttgaa aaacacgatg ataaggatcc
     4861 accggagctt accatgaccg agtacaagcc cacggtgcgc ctcgccaccc gcgacgacgt
     4921 ccccagggcc gtacgcaccc tcgccgccgc gttcgccgac taccccgcca cgcgccacac
     4981 cgtcgatccg gaccgccaca tcgagcgggt caccgagctg caagaactct tcctcacgcg
     5041 cgtcgggctc gacatcggca aggtgtgggt cgcggacgac ggcgccgcgg tggcggtctg
     5101 gaccacgccg gagagcgtcg aagcgggggc ggtgttcgcc gagatcggcc cgcgcatggc
     5161 cgagttgagc ggttcccggc tggccgcgca gcaacagatg gaaggcctcc tggcgccgca
     5221 ccggcccaag gagcccgcgt ggttcctggc caccgtcggc gtctcgcccg accaccaggg
     5281 caagggtctg ggcagcgccg tcgtgctccc cggagtggag gcggccgagc gcgccggggt
     5341 gcccgccttc ctggagacct ccgcgccccg caacctcccc ttctacgagc ggctcggctt
     5401 caccgtcacc gccgacgtcg aggtgcccga aggaccgcgc acctggtgca tgacccgcaa
     5461 gcccggtgcc tgacgcccgc cccacgaccc gcagcgcccg accgaaagga gcgcacgacc
     5521 ccatgcatcg gtacctttaa gaccaatgac ttacaaggca gctgtagatc ttagccactt
     5581 tttaaaagaa aaggggggac tggaagggct aattcactcc caacgaagac aagatctgct
     5641 ttttgcttgt actgggtctc tctggttaga ccagatctga gcctgggagc tctctggcta
     5701 actagggaac ccactgctta agcctcaata aagcttgcct tgagtgcttc aagtagtgtg
     5761 tgcccgtctg ttgtgtgact ctggtaacta gagatccctc agaccctttt agtcagtgtg
     5821 gaaaatctct agcagtagta gttcatgtca tcttattatt cagtatttat aacttgcaaa
     5881 gaaatgaata tcagagagtg agaggaactt gtttattgca gcttataatg gttacaaata
     5941 aagcaatagc atcacaaatt tcacaaataa agcatttttt tcactgcatt ctagttgtgg
     6001 tttgtccaaa ctcatcaatg tatcttatca tgtctggctc tagctatccc gcccctaact
     6061 ccgcccatcc cgcccctaac tccgcccagt tccgcccatt ctccgcccca tggctgacta
     6121 atttttttta tttatgcaga ggccgaggcc gcctcggcct ctgagctatt ccagaagtag
     6181 tgaggaggct tttttggagg cctagggacg tacccaattc gccctatagt gagtcgtatt
     6241 acgcgcgctc actggccgtc gttttacaac gtcgtgactg ggaaaaccct ggcgttaccc
     6301 aacttaatcg ccttgcagca catccccctt tcgccagctg gcgtaatagc gaagaggccc
     6361 gcaccgatcg cccttcccaa cagttgcgca gcctgaatgg cgaatgggac gcgccctgta
     6421 gcggcgcatt aagcgcggcg ggtgtggtgg ttacgcgcag cgtgaccgct acacttgcca
     6481 gcgccctagc gcccgctcct ttcgctttct tcccttcctt tctcgccacg ttcgccggct
     6541 ttccccgtca agctctaaat cgggggctcc ctttagggtt ccgatttagt gctttacggc
     6601 acctcgaccc caaaaaactt gattagggtg atggttcacg tagtgggcca tcgccctgat
     6661 agacggtttt tcgccctttg acgttggagt ccacgttctt taatagtgga ctcttgttcc
     6721 aaactggaac aacactcaac cctatctcgg tctattcttt tgatttataa gggattttgc
     6781 cgatttcggc ctattggtta aaaaatgagc tgatttaaca aaaatttaac gcgaatttta
     6841 acaaaatatt aacgcttaca atttaggtgg cacttttcgg ggaaatgtgc gcggaacccc
     6901 tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg
     6961 ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc
     7021 ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt
     7081 gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct
     7141 caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac
     7201 ttttaaagtt ctgctatgtg gcgcggtatt atcccgtatt gacgccgggc aagagcaact
     7261 cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa
     7321 gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga
     7381 taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc taaccgcttt
     7441 tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga
     7501 agccatacca aacgacgagc gtgacaccac gatgcctgta gcaatggcaa caacgttgcg
     7561 caaactatta actggcgaac tacttactct agcttcccgg caacaattaa tagactggat
     7621 ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat
     7681 tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag cactggggcc
     7741 agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga
     7801 tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaactgtc
     7861 agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag
     7921 gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc
     7981 gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt
     8041 tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt
     8101 gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat
     8161 accaaatact gttcttctag tgtagccgta gttaggccac cacttcaaga actctgtagc
     8221 accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa
     8281 gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg
     8341 ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag
     8401 atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag
     8461 gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa
     8521 cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt
     8581 gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg
     8641 gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc
     8701 tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac
     8761 cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc ccaatacgca aaccgcctct
     8821 cc
//
