LOCUS       sequence-212676-        3509 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Bacterial expression of cherry-NanoLuc.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3509)
  AUTHORS   Botero S, Chiaroni-Clarke R, Simon SM
  TITLE     Escherichia coli as a platform for the study of phosphoinositide 
            biology.
  JOURNAL   Sci Adv. 2019 Mar 27;5(3):eaat4872. doi: 10.1126/sciadv.aat4872. 
            eCollection 2019 Mar.
  PUBMED    30944849
REFERENCE   2  (bases 1 to 3509)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Adv."; 
            date: "2019-03-27"; volume: "5(3):eaat4872. doi"; pages: " 
            10.1126/sciadv.aat4872. eCollection 2019 Mar"
COMMENT     Sequence Label: pClodANL-Cherry
FEATURES             Location/Qualifiers
     source          1..3509
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(9..747)
                     /direction=LEFT
                     /label=CloDF13 ori
                     /note="Plasmids containing the CloDF13 (CDF) origin of 
                     replication can be propagated in E. coli cells that contain
                     additional plasmids with compatible origins."
     RBS             952..974
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             1514..1525
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(2311..2329)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        2397..2501
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(2502..2570,2571..3362)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(2720..2739)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 aagatctagc gctgcggaca catacaaagt tgcccacaga ttccgtggat aagcagggga
       61 ctaacatgtg aggcaaaaca gcagggccgc gccggtggcg tttttccata ggctccgccc
      121 tcctgccaga gttcacataa acagacgctt ttccggtgca tctgtgggag ccgtgaggct
      181 caaccatgaa tctgacagta cgggcgaaac ccgacaggac ttaaagatcc ccaccgtttc
      241 cggcgggtcg ctccctcttg cgctctcctg ttccgaccct gccgtttacc ggatacctgt
      301 tccgcctttc tcccttacgg gaagtgtggc gctttctcat agctcacaca ctggtatctc
      361 ggctcggtgt aggtcgttcg ctccaagctg ggctgtaagc aagaactccc cgttcagccc
      421 gactgctgcg ccttatccgg taactgttca cttgagtcca acccggaaaa gcacggtaaa
      481 acgccactgg cagcagccat tggtaactgg gagttcgcag aggatttgtt tagctaaaca
      541 cgcggttgct cttgaagtgt gcgccaaagt ccggctacac tggaaggaca gatttggttg
      601 ctgtgctctg cgaaagccag ttaccacggt taagcagttc cccaactgac ttaaccttcg
      661 atcaaaccac ctccccaggt ggttttttcg tttacagggc aaaagattac gcgcagaaaa
      721 aaaggatctc aagaagatcc tttgatcagc ggccgcaaaa aaggtacctg ccatgtatac
      781 tagaccttaa ttaacacagc taacaccacg tcgtccctat ctgctgccct aggtctatga
      841 gtggttgctg gataacttta cgggcatgca taaggctcgt ataatatatt cagggagacc
      901 acaacggttt ccctctacaa ataattttgt ttaactttga gggacaataa ttttgtttaa
      961 ctttaagaag gagatataca ttactagtat cgatgtctag aggggtctca aagggtgaag
     1021 aggacaacat ggcaataatt aaagagttca tgagatttaa agtccatatg gagggttcag
     1081 tcaacggaca cgagttcgag attgagggtg agggagaggg aaggccatac gagggaactc
     1141 agactgcaaa gttgaaggtc acaaaaggtg gacctttacc attcgcatgg gacatattat
     1201 caccacagtt tatgtatggt tctaaggcat acgtcaagca cccagctgat attccagact
     1261 acttgaagtt gtcttttcct gaaggtttca aatgggagag ggtcatgaac ttcgaggacg
     1321 gaggagtcgt cactgtcact caggactcat ctttgcagga tggagagttt atatacaaag
     1381 ttaaattgag gggaacaaac ttcccatcag acggaccagt catgcaaaag aagactatgg
     1441 gttgggaggc ttcttcagag aggatgtacc cagaggacgg agctttgaag ggtgagataa
     1501 aacaaaggtt gaaattgaag gacggtggtc actacgacgc tgaagttaag acaacataca
     1561 aggctaagaa gcctgtccaa ttgcctggtg cttataacgt caacattaaa ttggacatta
     1621 cttctcacaa cgaggattac actattgtcg agcaatacga gagggctgag ggaagacact
     1681 ctactggagg tggcagtgat gggactagag gagtttttac tctggaagat tttgttggcg
     1741 attggcgtca gaccgcgggt tataatttgg atcaagtcct ggaacagggt ggcgtaagct
     1801 ctctgttcca gaacctgggt gtgagcgtga cgccgattca gcgcatcgtt ctgtccggcg
     1861 agaacggtct gaaaattgat attcatgtga tcatcccgta cgaaggcctg agcggtgacc
     1921 aaatgggtca aatcgagaaa atctttaaag tcgtctaccc agttgacgat caccacttca
     1981 aggttatctt gcattacggt acgctggtga ttgatggtgt gaccccgaat atgattgact
     2041 atttcggccg tccgtatgaa ggcattgccg tttttgacgg taaaaagatc accgtcaccg
     2101 gtaccctgtg gaatggcaat aagattattg acgagcgtct gattaacccg gacggcagcc
     2161 tgctgttccg cgtgaccatc aacggtgtca cgggttggcg tctgtgcgag cgcatcctgg
     2221 cataataagt cgacgttaat aacaggcctg ctggtaatcg caggcctttt tatttttttt
     2281 ttttgctagc aaattcttga agacgaaagg gcctcgtgat acgcctattt ttataggtta
     2341 atgtcatgat aataatggtt tcttagacgt caggtggcac ttttcgggga aatgtgcgcg
     2401 gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat
     2461 aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc
     2521 gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa
     2581 cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac
     2641 tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttccaatga
     2701 tgagcacttt taaagttctg ctatgtggcg cggtattatc ccgtgttgac gccgggcaag
     2761 agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtac tcaccagtca
     2821 cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct gccataacca
     2881 tgagtgataa cactgcggcc aacttacttc tgacaacgat cggaggaccg aaggagctaa
     2941 ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg gaaccggagc
     3001 tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgcagca atggcaacaa
     3061 cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa caattaatag
     3121 actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt ccggctggct
     3181 ggtttattgc tgataaatct ggagccggtg agcgtgggtc tcgcggtatc attgcagcac
     3241 tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa
     3301 ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt
     3361 aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat
     3421 ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg
     3481 agttttcgtt ccactgagcg tcagacccc
//
