LOCUS       sequence-212678-       14550 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses FANCD2..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 14550)
  AUTHORS   Richardson CD, Kazane KR, Feng SJ, Zelin E, Bray NL, Schafer AJ, 
            Floor SN, Corn JE
  TITLE     CRISPR-Cas9 genome editing in human cells occurs via the Fanconi 
            anemia pathway.
  JOURNAL   Nat Genet. 2018 Aug;50(8):1132-1139. doi: 10.1038/s41588-018-0174-0.
            Epub 2018 Jul 27.
  PUBMED    30054595
REFERENCE   2  (bases 1 to 14550)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41588-018-0174-0"; journalName: "Nat Genet"; date: 
            "2018-08"; volume: "50"; issue: "8"; pages: "1132-1139"
COMMENT     Sequence Label: pCR1317
FEATURES             Location/Qualifiers
     source          1..14550
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        45..149
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(150..218,219..1010)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(368..387)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1181..1769
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1670..1689
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1923..1940
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    2057..2078
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(2093..2098,2099..2116,2117..2123)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2131..2147
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     2136..2158
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     2155..2171
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     2155..2171
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     2190..2210
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        2192..2210
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        2238..2464
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             2465..2645
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    2692..2817
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    3310..3543
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             3728..3772
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    4039..4155
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     protein_bind    4189..4213
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     promoter        4344..5522
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          4575..5513
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     5470..5490
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     promoter        5539..5557
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             5604..5630
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     primer_bind     complement(10244..10261)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     10471..10490
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     regulatory      10646..10655
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             10658..11146
                     /codon_start=1
                     /product="mouse GPI-anchored cell surface glycoprotein of 
                     the immunoglobulin superfamily"
                     /label=Thy1.1
                     /note="This allele of Thy1 is also known as CD90.1."
                     /translation="MNPAISVALLLSVLQVSRGQKVTSLTACLVNQNLRLDCRHENNTK
                     DNSIQHEFSLTREKRKHVLSGTLGIPEHTYRSRVTLSNQPYIKVLTLANFTTKDEGDYF
                     CELRVSGANPMSSNKSISVYRDKLVKCGGISLLVQNTSWMLLLLLSLSLLQALDFISL"
     protein_bind    complement(11152..11176)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     misc_feature    11207..11795
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(11260..11280)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(11678..11689)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     promoter        11846..12345
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(11881..11904)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     12278..12297
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             12359..13162
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MGSAIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQ
                     GRPVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQD
                     LLSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDE
                     EHQGLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQ
                     DIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(12422..12441)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     13032..13051
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     LTR             13306..13539
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    13611..13732
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(13648..13667)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     13702..13721
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      13772..13907
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     promoter        complement(13928..13946)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(13956..13973)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(13956..13972)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(13965..13987)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      14114..14542
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(14201..14220)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     14411..14432
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
ORIGIN
        1 atattaacgc ttacaattta ggtggcactt ttcggggaaa tgtgcgcgga acccctattt
       61 gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa
      121 tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta
      181 ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag
      241 taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca
      301 gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta
      361 aagttctgct atgtggcgcg gtattatccc gtattgacgc cgggcaagag caactcggtc
      421 gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc
      481 ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca
      541 ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc
      601 acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca
      661 taccaaacga cgagcgtgac accacgatgc ctgtagcaat ggcaacaacg ttgcgcaaac
      721 tattaactgg cgaactactt actctagctt cccggcaaca attaatagac tggatggagg
      781 cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg
      841 ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg
      901 gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac
      961 gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc
     1021 aagtttactc atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct
     1081 aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc
     1141 actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc
     1201 gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg
     1261 atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa
     1321 atactgttct tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc
     1381 ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt
     1441 gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa
     1501 cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc
     1561 tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc
     1621 cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct
     1681 ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat
     1741 gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc
     1801 tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg
     1861 ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc
     1921 gcagcgagtc agtgagcgag gaagcggaag agcgcccaat acgcaaaccg cctctccccg
     1981 cgcgttggcc gattcattaa tgcagctggc acgacaggtt tcccgactgg aaagcgggca
     2041 gtgagcgcaa cgcaattaat gtgagttagc tcactcatta ggcaccccag gctttacact
     2101 ttatgcttcc ggctcgtatg ttgtgtggaa ttgtgagcgg ataacaattt cacacaggaa
     2161 acagctatga ccatgattac gccaagcgcg caattaaccc tcactaaagg gaacaaaagc
     2221 tggagctgca agcttaatgt agtcttatgc aatactcttg tagtcttgca acatggtaac
     2281 gatgagttag caacatgcct tacaaggaga gaaaaagcac cgtgcatgcc gattggtgga
     2341 agtaaggtgg tacgatcgtg ccttattagg aaggcaacag acgggtctga catggattgg
     2401 acgaaccact gaattgccgc attgcagaga tattgtattt aagtgcctag ctcgatacat
     2461 aaacgggtct ctctggttag accagatctg agcctgggag ctctctggct aactagggaa
     2521 cccactgctt aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct
     2581 gttgtgtgac tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc
     2641 tagcagtggc gcccgaacag ggacttgaaa gcgaaaggga aaccagagga gctctctcga
     2701 cgcaggactc ggcttgctga agcgcgcacg gcaagaggcg aggggcggcg actggtgagt
     2761 acgccaaaaa ttttgactag cggaggctag aaggagagag atgggtgcga gagcgtcagt
     2821 attaagcggg ggagaattag atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag
     2881 aaaaaatata aattaaaaca tatagtatgg gcaagcaggg agctagaacg attcgcagtt
     2941 aatcctggcc tgttagaaac atcagaaggc tgtagacaaa tactgggaca gctacaacca
     3001 tcccttcaga caggatcaga agaacttaga tcattatata atacagtagc aaccctctat
     3061 tgtgtgcatc aaaggataga gataaaagac accaaggaag ctttagacaa gatagaggaa
     3121 gagcaaaaca aaagtaagac caccgcacag caagcggccg ctgatcttca gacctggagg
     3181 aggagatatg agggacaatt ggagaagtga attatataaa tataaagtag taaaaattga
     3241 accattagga gtagcaccca ccaaggcaaa gagaagagtg gtgcagagag aaaaaagagc
     3301 agtgggaata ggagctttgt tccttgggtt cttgggagca gcaggaagca ctatgggcgc
     3361 agcgtcaatg acgctgacgg tacaggccag acaattattg tctggtatag tgcagcagca
     3421 gaacaatttg ctgagggcta ttgaggcgca acagcatctg ttgcaactca cagtctgggg
     3481 catcaagcag ctccaggcaa gaatcctggc tgtggaaaga tacctaaagg atcaacagct
     3541 cctggggatt tggggttgct ctggaaaact catttgcacc actgctgtgc cttggaatgc
     3601 tagttggagt aataaatctc tggaacagat ttggaatcac acgacctgga tggagtggga
     3661 cagagaaatt aacaattaca caagcttaat acactcctta attgaagaat cgcaaaacca
     3721 gcaagaaaag aatgaacaag aattattgga attagataaa tgggcaagtt tgtggaattg
     3781 gtttaacata acaaattggc tgtggtatat aaaattattc ataatgatag taggaggctt
     3841 ggtaggttta agaatagttt ttgctgtact ttctatagtg aatagagtta ggcagggata
     3901 ttcaccatta tcgtttcaga cccacctccc aaccccgagg ggacccgaca ggcccgaagg
     3961 aatagaagaa gaaggtggag agagagacag agacagatcc attcgattag tgaacggatc
     4021 tcgacggtat cggttaactt ttaaaagaaa aggggggatt ggggggtaca gtgcagggga
     4081 aagaatagta gacataatag caacagacat acaaactaaa gaattacaaa aacaaattac
     4141 aaaattcaaa attttatcgt ccagtgtggt ggaattctgc agatatcaac aagtttgtac
     4201 aaaaaagcag gctccaccat gggaaccgac attgattatt gactaggctt ttgcaaaaag
     4261 ctttgcaaag atggataaag ttttaaacag agaggaatct ttgcagctaa tggaccttct
     4321 aggtcttgaa aggagtggga attggctccg gtgcccgtca gtgggcagag cgcacatcgc
     4381 ccacagtccc cgagaagttg gggggagggg tcggcaattg aaccggtgcc tagagaaggt
     4441 ggcgcggggt aaactgggaa agtgatgtcg tgtactggct ccgccttttt cccgagggtg
     4501 ggggagaacc gtatataagt gcagtagtcg ccgtgaacgt tctttttcgc aacgggtttg
     4561 ccgccagaac acaggtaagt gccgtgtgtg gttcccgcgg gcctggcctc tttacgggtt
     4621 atggcccttg cgtgccttga attacttcca cctggctgca gtacgtgatt cttgatcccg
     4681 agcttcgggt tggaagtggg tgggagagtt cgaggccttg cgcttaagga gccccttcgc
     4741 ctcgtgcttg agttgaggcc tggcctgggc gctggggccg ccgcgtgcga atctggtggc
     4801 accttcgcgc ctgtctcgct gctttcgata agtctctagc catttaaaat ttttgatgac
     4861 ctgctgcgac gctttttttc tggcaagata gtcttgtaaa tgcgggccaa gatctgcaca
     4921 ctggtatttc ggtttttggg gccgcgggcg gcgacggggc ccgtgcgtcc cagcgcacat
     4981 gttcggcgag gcggggcctg cgagcgcggc caccgagaat cggacggggg tagtctcaag
     5041 ctggccggcc tgctctggtg cctggcctcg cgccgccgtg tatcgccccg ccctgggcgg
     5101 caaggctggc ccggtcggca ccagttgcgt gagcggaaag atggccgctt cccggccctg
     5161 ctgcagggag ctcaaaatgg aggacgcggc gctcgggaga gcgggcgggt gagtcaccca
     5221 cacaaaggaa aagggccttt ccgtcctcag ccgtcgcttc atgtgactcc acggagtacc
     5281 gggcgccgtc caggcacctc gattagttct cgagcttttg gagtacgtcg tctttaggtt
     5341 ggggggaggg gttttatgcg atggagtttc cccacactga gtgggtggag actgaagtta
     5401 ggccagcttg gcacttgatg taattctcct tggaatttgc cctttttgag tttggatctt
     5461 ggttcattct caagcctcag acagtggttc aaagtttttt tcttccattt caggtgtcgt
     5521 gaggaattag cttggtacta atacgactca ctatagggag acccaagctg gctaggtaag
     5581 cttggtaccg agctcggatc atgtacccat acgatgttcc agattacgct ggtggatctg
     5641 gaggttctgg tggaatggtt tccaaaagaa gactgtcaaa atctgaggat aaagagagcc
     5701 tgacagaaga tgcctccaaa accaggaagc aaccactttc caaaaagaca aagaaatctc
     5761 atattgctaa tgaagttgaa gaaaatgaca gcatctttgt aaagcttctt aagatatcag
     5821 gaattattct taaaacggga gagagtcaga atcaactagc tgtggatcaa atagctttcc
     5881 aaaagaagct ctttcagacc ctgaggagac acccttccta tcccaaaata atagaagaat
     5941 ttgttagtgg cctggagtct tacattgagg atgaagacag tttcaggaac tgccttttgt
     6001 cttgtgagcg tctgcaggat gaggaagcca gtatgggtgc atcttattct aagagtctca
     6061 tcaaactgct tctggggatt gacatactgc agcctgccat tatcaaaacc ttatttgaga
     6121 agttgccaga atattttttt gaaaacaaga acagtgatga aatcaacata cctcgactca
     6181 ttgtcagtca actaaaatgg cttgacagag ttgtggatgg caaggacctc accaccaaga
     6241 tcatgcagct gatcagtatt gctccagaga acctgcagca tgacatcatc accagcctac
     6301 ctgagatcct aggggattcc cagcacgctg atgtggggaa agaactcagt gacctactga
     6361 tagagaatac ttcactcact gtcccaatcc tggatgtcct ttcaagcctc cgacttgacc
     6421 caaacttcct attgaaggtt cgccagttgg tgatggataa gttgtcgtct attagattgg
     6481 aggatttacc tgtgataata aagttcattc ttcattccgt aacagccatg gatacacttg
     6541 aggtaatttc tgagcttcgg gagaagttgg atctgcagca ttgtgttttg ccatcacggt
     6601 tacaggcttc ccaagtaaag ttgaaaagta aaggacgagc aagttcctca ggaaatcaag
     6661 aaagcagcgg tcagagctgt attattctcc tctttgatgt aataaagtca gctattagat
     6721 atgagaaaac catttcagaa gcctggatta aggcaattga aaacactgcc tcagtatctg
     6781 aacacaaggt gtttgacctg gtgatgcttt tcatcatcta tagcaccaat actcagacaa
     6841 agaagtacat tgacagggtg ctaagaaata agattcgatc aggctgcatt caagaacagc
     6901 tgctccagag tacattctct gttcattact tagttcttaa ggatatgtgt tcatccattc
     6961 tgtcgctggc tcagagtttg cttcactctc tagaccagag tataatttca tttggcagtc
     7021 tcctatacaa atatgcattt aagttttttg acacgtactg ccagcaggaa gtggttggtg
     7081 ccttagtgac ccatatctgc agtgggaatg aagctgaagt tgatactgcc ttagatgtcc
     7141 ttctagagtt ggtagtgtta aacccatctg ctatgatgat gaatgctgtc tttgtaaagg
     7201 gcattttaga ttatctggat aacatatccc ctcagcaaat acgaaaactc ttctatgttc
     7261 tcagcacact ggcatttagc aaacagaatg aagccagcag ccacatccag gatgacatgc
     7321 acttggtgat aagaaagcag ctctctagca ccgtattcaa gtacaagctc attgggatta
     7381 ttggtgctgt gaccatggct ggcatcatgg cggcagacag aagtgaatca cctagtttga
     7441 cccaagagag agccaacctg agcgatgagc agtgcacaca ggtgacctcc ttgttgcagt
     7501 tggttcattc ctgcagtgag cagtctcctc aggcctctgc actttactat gatgaatttg
     7561 ccaacctgat ccaacatgaa aagctggatc caaaagccct ggaatgggtt gggcatacca
     7621 tctgtaatga tttccaggat gccttcgtag tggactcctg tgttgttccg gaaggtgact
     7681 ttccatttcc tgtgaaagca ctgtacggac tggaagaata cgacactcag gatgggattg
     7741 ccataaacct cctgccgctg ctgttttctc aggactttgc aaaagatggg ggtccggtga
     7801 cctcacagga atcaggccaa aaattggtgt ctccgctgtg cctggctccg tatttccggt
     7861 tactgagact ttgtgtggag agacagcata acggaaactt ggaggagatt gatggtctac
     7921 tagattgtcc tatattccta actgacctgg agcctggaga gaagttggag tccatgtctg
     7981 ctaaagagcg ttcattcatg tgttctctca tatttcttac tctcaactgg ttccgagaga
     8041 ttgtaaatgc cttctgccag gaaacatcac ctgagatgaa ggggaaggtg ctcactcggt
     8101 taaagcacat tgtagaattg caaataatcc tggaaaagta cttggcagtc accccagact
     8161 atgtccctcc tcttggaaac tttgatgtgg aaactttaga tataacacct catactgtta
     8221 ctgctatttc agcaaaaatc agaaagaaag gaaaaataga aaggaaacaa aaaacagatg
     8281 gcagcaagac atcctcctct gacacacttt cagaagagaa aaattcagaa tgtgacccta
     8341 cgccatctca tagaggccag ctaaacaagg agttcacagg gaaggaagaa aagacatcat
     8401 tgttactaca taattcccat gcttttttcc gagagctgga cattgaggtc ttctctattc
     8461 tacattgtgg acttgtgacg aagttcatct tagatactga aatgcacact gaagctacag
     8521 aagttgtgca acttgggccc cctgagctgc ttttcttgct ggaagatctc tcccagaagc
     8581 tggagagtat gctgacacct cctattgcca ggagagtccc ctttctcaag aacaaaggaa
     8641 gccggaatat tggattctca catctccaac agagatctgc ccaagaaatt gttcattgtg
     8701 tttttcaact gctgacccca atgtgtaacc acctggagaa cattcacaac tattttcagt
     8761 gtttagctgc tgagaatcac ggtgtagttg atggaccagg agtgaaagtt caggagtacc
     8821 acataatgtc ttcctgctat cagaggctgc tgcagatttt tcatgggctt tttgcttgga
     8881 gtggattttc tcaacctgaa aatcagaatt tactgtattc agccctccat gtccttagta
     8941 gccgactgaa acagggagaa cacagccagc ctttggagga actactcagc cagagcgtcc
     9001 attacttgca gaatttccat caaagcattc ccagtttcca gtgtgctctt tatctcatca
     9061 gacttttgat ggttattttg gagaaatcaa cagcttctgc tcagaacaaa gaaaaaattg
     9121 cttcccttgc cagacaattc ctctgtcggg tgtggccaag tggggataaa gagaagagca
     9181 acatctctaa tgaccagctc catgctctgc tctgtatcta cctggagcac acagagagca
     9241 ttctgaaggc catagaggag attgctggtg ttggtgtccc agaactgatc aactctccta
     9301 aagatgcatc ttcctccaca ttccctacac tgaccaggca tacttttgtt gttttcttcc
     9361 gtgtgatgat ggctgaacta gagaagacgg tgaaaaaaat tgagcctggc acagcagcag
     9421 actcgcagca gattcatgaa gagaaactcc tctactggaa catggctgtt cgagacttca
     9481 gtatcctcat caacttgata aaggtatttg atagtcatcc tgttctgcat gtatgtttga
     9541 agtatgggcg tctctttgtg gaagcatttc tgaagcaatg tatgccgctc ctagacttca
     9601 gttttagaaa acaccgggaa gatgttctga gcttactgga aaccttccag ttggacacaa
     9661 ggctgcttca tcacctgtgt gggcattcca agattcacca ggacacgaga ctcacccaac
     9721 atgtgcctct gctcaaaaag accctggaac ttttagtttg cagagtcaaa gctatgctca
     9781 ctctcaacaa ttgtagagag gctttctggc tgggcaatct aaaaaaccgg gacttgcagg
     9841 gtgaagagat taagtcccaa aattcccagg agagcacagc agatgagagt gaggatgaca
     9901 tgtcatccca ggcctccaag agcaaagcca ctgaggtatc tctacaaaac ccaccagagt
     9961 ctggcactga tggttgcatt ttgttaattg ttctaagttg gtggagcaga actttgccta
    10021 cttatgttta ttgtcaaatg cttctatgcc catttccatt ccctccataa gtcgaccccc
    10081 tctccctccc ccccccctaa cgttactggc cgaagccgct tggaataagg ccggtgtgcg
    10141 tttgtctata tgttattttc caccatattg ccgtcttttg gcaatgtgag ggcccggaaa
    10201 cctggccctg tcttcttgac gagcattcct aggggtcttt cccctctcgc caaaggaatg
    10261 caaggtctgt tgaatgtcgt gaaggaagca gttcctctgg aagcttcttg aagacaaaca
    10321 acgtctgtag cgaccctttg caggcagcgg aaccccccac ctggcgacag gtgcctctgc
    10381 ggccaaaagc cacgtgtata agatacacct gcaaaggcgg cacaacccca gtgccacgtt
    10441 gtgagttgga tagttgtgga aagagtcaaa tggctctcct caagcgtatt caacaagggg
    10501 ctgaaggatg cccagaaggt accccattgt atgggatctg atctggggcc tcggtgcaca
    10561 tgctttacat gtgtttagtc gaggttaaaa aaacgtctag gccccccgaa ccacggggac
    10621 gtggttttcc tttgaaaaac acgatgccac catgggtatg aacccagcca tcagcgtcgc
    10681 tctcctgctc tcagtcttgc aggtgtcccg agggcagaag gtgaccagcc tgacagcctg
    10741 cctggtgaac caaaaccttc gcctggactg ccgccatgag aataacacca aggataactc
    10801 catccagcat gagttcagcc tgacccgaga gaagaggaag cacgtgctct caggcaccct
    10861 tgggataccc gagcacacgt accgctcccg cgtcaccctc tccaaccagc cctatatcaa
    10921 ggtccttacc ctagccaact tcaccaccaa ggatgagggc gactactttt gtgagcttcg
    10981 cgtctcgggc gcgaatccca tgagctccaa taaaagtatc agtgtgtata gagacaagct
    11041 ggtcaagtgt ggcggcataa gcctgctggt tcagaacaca tcctggatgc tgctgctgct
    11101 gctttccctc tccctcctcc aagccctgga cttcatttct ctgtgatcta gacccagctt
    11161 tcttgtacaa agtggttgat atccagcaca gtggcggccg ctcgacaatc aacctctgga
    11221 ttacaaaatt tgtgaaagat tgactggtat tcttaactat gttgctcctt ttacgctatg
    11281 tggatacgct gctttaatgc ctttgtatca tgctattgct tcccgtatgg ctttcatttt
    11341 ctcctccttg tataaatcct ggttgctgtc tctttatgag gagttgtggc ccgttgtcag
    11401 gcaacgtggc gtggtgtgca ctgtgtttgc tgacgcaacc cccactggtt ggggcattgc
    11461 caccacctgt cagctccttt ccgggacttt cgctttcccc ctccctattg ccacggcgga
    11521 actcatcgcc gcctgccttg cccgctgctg gacaggggct cggctgttgg gcactgacaa
    11581 ttccgtggtg ttgtcgggga agctgacgtc ctttccatgg ctgctcgcct gtgttgccac
    11641 ctggattctg cgcgggacgt ccttctgcta cgtcccttcg gccctcaatc cagcggacct
    11701 tccttcccgc ggcctgctgc cggctctgcg gcctcttccg cgtcttcgcc ttcgccctca
    11761 gacgagtcgg atctcccttt gggccgcctc cccgcctgga cctgcaggat tctgcagata
    11821 tccggactag tgatctaatt ctaccgggta ggggaggcgc ttttcccaag gcagtctgga
    11881 gcatgcgctt tagcagcccc gctgggcact tggcgctaca caagtggcct ctggcctcgc
    11941 acacattcca catccaccgg taggcgccaa ccggctccgt tctttggtgg ccccttcgcg
    12001 ccaccttcta ctcctcccct agtcaggaag ttcccccccg ccccgcagct cgcgtcgtgc
    12061 aggacgtgac aaatggaagt agcacgtctc actagtctcg tgcagatgga cagcaccgct
    12121 gagcaatgga agcgggtagg cctttggggc agcggccaat agcagctttg ctccttcgct
    12181 ttctgggctc agaggctggg aaggggtggg tccgggggcg ggctcagggg cgggctcagg
    12241 ggcggggcgg gcgcccgaag gtcctccgga ggcccggcat tctgcacgct tcaaaagcgc
    12301 acgtctgccg cgctgttctc ctcttcctca tctccgggcc tttcgacctg cagccaatat
    12361 gggatcggcc attgaacaag atggattgca cgcaggttct ccggccgctt gggtggagag
    12421 gctattcggc tatgactggg cacaacagac aatcggctgc tctgatgccg ccgtgttccg
    12481 gctgtcagcg caggggcgcc cggttctttt tgtcaagacc gacctgtccg gtgccctgaa
    12541 tgaactgcag gacgaggcag cgcggctatc gtggctggcc acgacgggcg ttccttgcgc
    12601 agctgtgctc gacgttgtca ctgaagcggg aagggactgg ctgctattgg gcgaagtgcc
    12661 ggggcaggat ctcctgtcat ctcaccttgc tcctgccgag aaagtatcca tcatggctga
    12721 tgcaatgcgg cggctgcata cgcttgatcc ggctacctgc ccattcgacc accaagcgaa
    12781 acatcgcatc gagcgagcac gtactcggat ggaagccggt cttgtcgatc aggatgatct
    12841 ggacgaagag catcaggggc tcgcgccagc cgaactgttc gccaggctca aggcgcgcat
    12901 gcccgacggc gaggatctcg tcgtgaccca tggcgatgcc tgcttgccga atatcatggt
    12961 ggaaaatggc cgcttttctg gattcatcga ctgtggccgg ctgggtgtgg cggaccgcta
    13021 tcaggacata gcgttggcta cccgtgatat tgctgaagag cttggcggcg aatgggctga
    13081 ccgcttcctc gtgctttacg gtatcgccgc tcccgattcg cagcgcatcg ccttctatcg
    13141 ccttcttgac gagttcttct gaacgcgtcg cccgccccac gacccgcagc gcccgaccga
    13201 aaggagcgca cgaccccatg catctcgagg gcccggtacc tttaagacca atgacttaca
    13261 aggcagctgt agatcttagc cactttttaa aagaaaaggg gggactggaa gggctagctc
    13321 actcccaacg aagacaagat ctgctttttg cttgtactgg gtctctctgg ttagaccaga
    13381 tctgagcctg ggagctctct ggctaactag ggaacccact gcttaagcct caataaagct
    13441 tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt aactagagat
    13501 ccctcagacc cttttagtca gtgtggaaaa tctctagcag tagtagttca tgtcatctta
    13561 ttattcagta tttataactt gcaaagaaat gaatatcaga gagtgagagg aacttgttta
    13621 ttgcagctta taatggttac aaataaagca atagcatcac aaatttcaca aataaagcat
    13681 ttttttcact gcattctagt tgtggtttgt ccaaactcat caatgtatct tatcatgtct
    13741 ggctctagct atcccgcccc taactccgcc catcccgccc ctaactccgc ccagttccgc
    13801 ccattctccg ccccatggct gactaatttt ttttatttat gcagaggccg aggccgcctc
    13861 ggcctctgag ctattccaga agtagtgagg aggctttttt ggaggcctag ggacgtaccc
    13921 aattcgccct atagtgagtc gtattacgcg cgctcactgg ccgtcgtttt acaacgtcgt
    13981 gactgggaaa accctggcgt tacccaactt aatcgccttg cagcacatcc ccctttcgcc
    14041 agctggcgta atagcgaaga ggcccgcacc gatcgccctt cccaacagtt gcgcagcctg
    14101 aatggcgaat gggacgcgcc ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg
    14161 cgcagcgtga ccgctacact tgccagcgcc ctagcgcccg ctcctttcgc tttcttccct
    14221 tcctttctcg ccacgttcgc cggctttccc cgtcaagctc taaatcgggg gctcccttta
    14281 gggttccgat ttagtgcttt acggcacctc gaccccaaaa aacttgatta gggtgatggt
    14341 tcacgtagtg ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg
    14401 ttctttaata gtggactctt gttccaaact ggaacaacac tcaaccctat ctcggtctat
    14461 tcttttgatt tataagggat tttgccgatt tcggcctatt ggttaaaaaa tgagctgatt
    14521 taacaaaaat ttaacgcgaa ttttaacaaa
//
