LOCUS       sequence-212685-        9871 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses a plasma membrane calcium pump hPMCA2x/b.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9871)
  AUTHORS   Yu X, Taylor AMW, Nagai J, Golshani P, Evans CJ, Coppola G, Khakh BS
  TITLE     Reducing Astrocyte Calcium Signaling In Vivo Alters Striatal 
            Microcircuits and Causes Repetitive Behavior.
  JOURNAL   Neuron. 2018 Aug 27. pii: S0896-6273(18)30688-3. doi: 
            10.1016/j.neuron.2018.08.015.
  PUBMED    30174118
REFERENCE   2  (bases 1 to 9871)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Neuron. 
            2018 Aug 27. pii: S0896-6273(18)30688-3. doi: 
            10.1016/j.neuron.2018.08.015."
COMMENT     Sequence Label: pcDNA3.1-CMV-mCherry-hPMCA2x/b
FEATURES             Location/Qualifiers
     source          1..9871
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        1..380
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        381..584
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     535..555
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     promoter        629..647
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        691..709
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             715..1422
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(860..878)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     1128..1147
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     CDS             1438..1464
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             1465..1482
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             1483..1500
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     regulatory      1552..1561
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             complement(2059..2070)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     regulatory      2527..2536
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(5228..5245)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    5234..5458
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      5504..5932
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5591..5610)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5801..5822
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(5941..5961)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        5946..6275
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      6126..6261
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     6188..6207
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             6342..7136
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(6396..6415)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     7006..7025
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    7310..7431
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7347..7366)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7401..7420
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(7480..7496)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7480..7496)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7493..7515)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7504..7520
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7528..7534,7535..7552,7553..7558))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7573..7594
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7711..7728)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7882..8470)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7962..7981)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8641..9432,9433..9501))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9264..9283
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9502..9606)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(9681..9700)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 gacattgatt attgactagt tattaatagt aatcaattac ggggtcatta gttcatagcc
       61 catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca
      121 acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga
      181 ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc
      241 aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct
      301 ggcattatgc ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat
      361 tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg cgtggatagc
      421 ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt
      481 ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa
      541 tgggcggtag gcgtgtacgg tgggaggtct atataagcag agctctctgg ctaactagag
      601 aacccactgc ttactggctt atcgaaatta atacgactca ctatagggag acccaagctg
      661 gctagcgttt aaacttaagg ctagagtact taatacgact cactataggc tagcatggtg
      721 agcaagggcg aggaggataa catggccatc atcaaggagt tcatgcgctt caaggtgcac
      781 atggagggct ccgtgaacgg ccacgagttc gagatcgagg gcgagggcga gggccgcccc
      841 tacgagggca cccagaccgc caagctgaag gtgaccaagg gtggccccct gcccttcgcc
      901 tgggacatcc tgtcccctca gttcatgtac ggctccaagg cctacgtgaa gcaccccgcc
      961 gacatccccg actacttgaa gctgtccttc cccgagggct tcaagtggga gcgcgtgatg
     1021 aacttcgagg acggcggcgt ggtgaccgtg acccaggact cctccctgca ggacggcgag
     1081 ttcatctaca aggtgaagct gcgcggcacc aacttcccct ccgacggccc cgtaatgcag
     1141 aagaagacca tgggctggga ggcctcctcc gagcggatgt accccgagga cggcgccctg
     1201 aagggcgaga tcaagcagag gctgaagctg aaggacggcg gccactacga cgctgaggtc
     1261 aagaccacct acaaggccaa gaagcccgtg cagctgcccg gcgcctacaa cgtcaacatc
     1321 aagttggaca tcacctccca caacgaggac tacaccatcg tggaacagta cgaacgcgcc
     1381 gagggccgcc actccaccgg cggcatggac gagctgtaca agtccggact cagatcctac
     1441 ccctatgatg tgcctgacta tgcccaccac caccaccacc acctggtgcc caggggcagc
     1501 agatctcgag ctcaagcttc gaattctgca gtcgacggta ccgcggtggc ggccgccatg
     1561 ggtgacatga ccaacagcga cttttactcc aaaaaccaaa gaaatgagtc gagccatggg
     1621 ggcgagttcg ggtgcacaat ggaggagctc cgctccctca tggagctgcg gggcactgag
     1681 gctgtggtca agatcaagga gacttatggg gacaccgaag ccatctgccg gcgcctcaaa
     1741 acctcacctg ttgaaggttt gccggacacc gctccagacc tggaaaagag aaagcaaatt
     1801 tttgggcaaa actttatacc tccaaagaag ccaaaaacct tcctgcagct cgtgtgggag
     1861 gcgctgcagg acgtgacgct catcatcctg gagattgccg ccatcatctc cctggggctg
     1921 tccttctacc acccgcccgg cgagggcaac gaaggatgtg cgacggccca gggtggggca
     1981 gaggatgaag gagaggcaga ggcaggttgg atcgaggggg ccgccattct cctctcagtt
     2041 atctgtgtgg tcctggtcac ggccttcaat gactggagca aagagaaaca gttccggggc
     2101 ctgcagagcc gcatcgagca ggaacagaaa tttaccgtag tccgggctgg ccaggtggtg
     2161 cagatccctg tggctgagat cgtggttggg gacatagccc aggtcaaata tggtgacctc
     2221 ctccctgccg acggcctctt catccagggc aatgacctca agattgatga aagctcccta
     2281 actggagagt ctgaccaggt gcgcaagtcc gtggacaagg accccatgct gctgtcagga
     2341 acccacgtga tggagggctc aggacggatg ttggtgactg ctgtgggtgt gaactctcag
     2401 actggcatca tctttaccct cctgggggct ggtggtgaag aggaagagaa gaaagacaaa
     2461 aaaggtaaaa tgcaggatgg caatgtggac gccagccaga gcaaagccaa acaacaggac
     2521 ggggcagccg ccatggagat gcagcccctc aagagtgccg agggcggcga cgctgacgac
     2581 aggaagaagg ccagcatgca caagaaggag aagtccgtgc tgcagggcaa gctcaccaag
     2641 ctggctgtgc agatcgggaa ggcgggcttg gtgatgtcag ccatcacggt gatcatcctg
     2701 gtgctctact tcactgtgga caccttcgtg gtcaacaaga agccgtggct gcctgagtgc
     2761 acgcccgtct acgtgcagta ctttgtcaag ttcttcatca ttggcgtgac ggtgctggtg
     2821 gtcgccgtgc ccgaggggct ccctctggcc gtcaccatct cgttggccta ttcggtgaag
     2881 aaaatgatga aggacaacaa cctggtacgc cacctggatg cctgtgagac catgggcaat
     2941 gccacagcca tctgctcaga caagacaggc acgctgacca ccaatcgcat gacagtggta
     3001 caggcctatg tcggcgacgt ccactataaa gagatccccg accccagctc catcaacacc
     3061 aagaccatgg agctgctgat caatgccatc gccatcaaca gcgcctacac caccaagatt
     3121 ctgcccccag agaaggaggg cgccctgcct cggcaggtgg gcaacaagac ggagtgcggc
     3181 ctgctgggct tcgtgctgga cctgaagcag gactacgagc ccgtgcgcag ccagatgcca
     3241 gaggagaagt tgtacaaagt gtacaccttc aactccgtgc gcaagtccat gagcactgtc
     3301 atcaagctgc ccgacgagag cttccgcatg tacagcaagg gggcttctga gatcgtgctc
     3361 aagaagtgct gcaaaatcct caatggggcg ggagagcctc gtgtcttccg gccccgcgac
     3421 cgggacgaga tggtaaagaa ggtgattgag cccatggctt gcgatgggct ccgcactatc
     3481 tgcgtggcct accgcgactt ccccagcagc ccggagccgg actgggacaa tgagaatgac
     3541 atcctcaacg aactcacctg catctgcgtg gtgggcatcg aggacccggt gcggccagag
     3601 gtcccagaag ccatccgcaa gtgccagcgg gcaggcatca cggtccgcat ggtcactggc
     3661 gacaatatca acacggctcg ggccatcgcc atcaagtgtg gcatcatcca tcctggggag
     3721 gactttctgt gcctcgaggg caaggagttc aacaggagga tccgcaacga gaagggggag
     3781 attgagcagg agcgaattga caagatctgg ccaaagctgc gggtgctggc tcgctcctcc
     3841 ccaacggaca agcataccct ggttaaaggc atcatcgaca gcacacacac tgagcagcgg
     3901 caggtggtgg ccgtgacggg ggacgggacc aacgacgggc ctgcactcaa gaaggccgac
     3961 gtgggcttcg ccatgggcat cgcaggcact gacgtggcca aggaggcctc agacatcatc
     4021 ctgacagacg acaatttcag cagcatcgtc aaggcagtga tgtggggccg caacgtctat
     4081 gacagcatct ccaaattctt gcagttccag ctcaccgtca acgtggtggc cgtgattgtg
     4141 gccttcacag gcgcctgcat cacgcaggac tcccctctga aggccgtgca gatgctctgg
     4201 gtgaacctca tcatggacac gtttgcctcg ctggcactgg ccactgagcc gcccacggag
     4261 accctgctgc tgaggaagcc gtacggccgc aacaagccgc tcatctccag gaccatgatg
     4321 aagaacatcc tgggccatgc tgtctaccag cttgccctca tcttcaccct gctctttgtt
     4381 ggcgagaaga tgttccagat cgacagcggg aggaacgcgc ccctgcattc gccaccctca
     4441 gaacattaca ccatcatctt caacaccttc gtcatgatgc agctcttcaa cgagatcaac
     4501 gcccgcaaga tccacggcga gcgcaatgtc tttgacggca tcttccggaa ccccatcttc
     4561 tgcaccatcg tgctgggcac ctttgccatc cagatagtga tcgtgcagtt tggagggaag
     4621 ccattcagct gctctccact gcagctggac cagtggatgt ggtgcatatt cattgggtta
     4681 ggagagctcg tttggggcca ggtcatcgcc accatcccga ccagcagact caagttcctc
     4741 aaggaggcag gcaggctcac acagaaggag gagatcccgg aggaggagct caacgaggac
     4801 gtggaggaga tcgaccacgc ggagcgggag ctgcggcggg gccagatcct gtggttccga
     4861 ggcctgaatc ggatccagac acagatccgc gtcgtgaagg cgttccgtag ctctctctat
     4921 gaaggtttag aaaagcctga atctcgaacc tccatccata acttcatggc tcatcctgaa
     4981 ttccggatcg aagattccca gccccacatc cccctcattg atgacaccga cctggaagaa
     5041 gatgccgcgc tcaagcagaa ctcgagcccg ccgtcatccc tcaacaagaa caacagcgcc
     5101 atcgacagtg ggatcaacct gacgaccgac acaagcaaat cagctacctc ttcaagtcca
     5161 gggagcccca tccacagcct ggagacgtcg ctttagtcta gagggcccgt ttaaacccgc
     5221 tgatcagcct cgactgtgcc ttctagttgc cagccatctg ttgtttgccc ctcccccgtg
     5281 ccttccttga ccctggaagg tgccactccc actgtccttt cctaataaaa tgaggaaatt
     5341 gcatcgcatt gtctgagtag gtgtcattct attctggggg gtggggtggg gcaggacagc
     5401 aagggggagg attgggaaga caatagcagg catgctgggg atgcggtggg ctctatggct
     5461 tctgaggcgg aaagaaccag ctggggctct agggggtatc cccacgcgcc ctgtagcggc
     5521 gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga ccgctacact tgccagcgcc
     5581 ctagcgcccg ctcctttcgc tttcttccct tcctttctcg ccacgttcgc cggctttccc
     5641 cgtcaagctc taaatcgggg gctcccttta gggttccgat ttagtgcttt acggcacctc
     5701 gaccccaaaa aacttgatta gggtgatggt tcacgtagtg ggccatcgcc ctgatagacg
     5761 gtttttcgcc ctttgacgtt ggagtccacg ttctttaata gtggactctt gttccaaact
     5821 ggaacaacac tcaaccctat ctcggtctat tcttttgatt tataagggat tttgccgatt
     5881 tcggcctatt ggttaaaaaa tgagctgatt taacaaaaat ttaacgcgaa ttaattctgt
     5941 ggaatgtgtg tcagttaggg tgtggaaagt ccccaggctc cccagcaggc agaagtatgc
     6001 aaagcatgca tctcaattag tcagcaacca ggtgtggaaa gtccccaggc tccccagcag
     6061 gcagaagtat gcaaagcatg catctcaatt agtcagcaac catagtcccg cccctaactc
     6121 cgcccatccc gcccctaact ccgcccagtt ccgcccattc tccgccccat ggctgactaa
     6181 ttttttttat ttatgcagag gccgaggccg cctctgcctc tgagctattc cagaagtagt
     6241 gaggaggctt ttttggaggc ctaggctttt gcaaaaagct cccgggagct tgtatatcca
     6301 ttttcggatc tgatcaagag acaggatgag gatcgtttcg catgattgaa caagatggat
     6361 tgcacgcagg ttctccggcc gcttgggtgg agaggctatt cggctatgac tgggcacaac
     6421 agacaatcgg ctgctctgat gccgccgtgt tccggctgtc agcgcagggg cgcccggttc
     6481 tttttgtcaa gaccgacctg tccggtgccc tgaatgaact gcaggacgag gcagcgcggc
     6541 tatcgtggct ggccacgacg ggcgttcctt gcgcagctgt gctcgacgtt gtcactgaag
     6601 cgggaaggga ctggctgcta ttgggcgaag tgccggggca ggatctcctg tcatctcacc
     6661 ttgctcctgc cgagaaagta tccatcatgg ctgatgcaat gcggcggctg catacgcttg
     6721 atccggctac ctgcccattc gaccaccaag cgaaacatcg catcgagcga gcacgtactc
     6781 ggatggaagc cggtcttgtc gatcaggatg atctggacga agagcatcag gggctcgcgc
     6841 cagccgaact gttcgccagg ctcaaggcgc gcatgcccga cggcgaggat ctcgtcgtga
     6901 cccatggcga tgcctgcttg ccgaatatca tggtggaaaa tggccgcttt tctggattca
     6961 tcgactgtgg ccggctgggt gtggcggacc gctatcagga catagcgttg gctacccgtg
     7021 atattgctga agagcttggc ggcgaatggg ctgaccgctt cctcgtgctt tacggtatcg
     7081 ccgctcccga ttcgcagcgc atcgccttct atcgccttct tgacgagttc ttctgagcgg
     7141 gactctgggg ttcgaaatga ccgaccaagc gacgcccaac ctgccatcac gagatttcga
     7201 ttccaccgcc gccttctatg aaaggttggg cttcggaatc gttttccggg acgccggctg
     7261 gatgatcctc cagcgcgggg atctcatgct ggagttcttc gcccacccca acttgtttat
     7321 tgcagcttat aatggttaca aataaagcaa tagcatcaca aatttcacaa ataaagcatt
     7381 tttttcactg cattctagtt gtggtttgtc caaactcatc aatgtatctt atcatgtctg
     7441 tataccgtcg acctctagct agagcttggc gtaatcatgg tcatagctgt ttcctgtgtg
     7501 aaattgttat ccgctcacaa ttccacacaa catacgagcc ggaagcataa agtgtaaagc
     7561 ctggggtgcc taatgagtga gctaactcac attaattgcg ttgcgctcac tgcccgcttt
     7621 ccagtcggga aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg cggggagagg
     7681 cggtttgcgt attgggcgct cttccgcttc ctcgctcact gactcgctgc gctcggtcgt
     7741 tcggctgcgg cgagcggtat cagctcactc aaaggcggta atacggttat ccacagaatc
     7801 aggggataac gcaggaaaga acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa
     7861 aaaggccgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa
     7921 tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc
     7981 ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc
     8041 cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta ggtatctcag
     8101 ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga
     8161 ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc
     8221 gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac
     8281 agagttcttg aagtggtggc ctaactacgg ctacactaga agaacagtat ttggtatctg
     8341 cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat ccggcaaaca
     8401 aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa
     8461 aggatctcaa gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa
     8521 ctcacgttaa gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt
     8581 aaattaaaaa tgaagtttta aatcaatcta aagtatatat gagtaaactt ggtctgacag
     8641 ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat
     8701 agttgcctga ctccccgtcg tgtagataac tacgatacgg gagggcttac catctggccc
     8761 cagtgctgca atgataccgc gagacccacg ctcaccggct ccagatttat cagcaataaa
     8821 ccagccagcc ggaagggccg agcgcagaag tggtcctgca actttatccg cctccatcca
     8881 gtctattaat tgttgccggg aagctagagt aagtagttcg ccagttaata gtttgcgcaa
     8941 cgttgttgcc attgctacag gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt
     9001 cagctccggt tcccaacgat caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc
     9061 ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag tgttatcact
     9121 catggttatg gcagcactgc ataattctct tactgtcatg ccatccgtaa gatgcttttc
     9181 tgtgactggt gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg
     9241 ctcttgcccg gcgtcaatac gggataatac cgcgccacat agcagaactt taaaagtgct
     9301 catcattgga aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc
     9361 cagttcgatg taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag
     9421 cgtttctggg tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac
     9481 acggaaatgt tgaatactca tactcttcct ttttcaatat tattgaagca tttatcaggg
     9541 ttattgtctc atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt
     9601 tccgcgcaca tttccccgaa aagtgccacc tgacgtcgac ggatcgggag atctcccgat
     9661 cccctatggt gcactctcag tacaatctgc tctgatgccg catagttaag ccagtatctg
     9721 ctccctgctt gtgtgttgga ggtcgctgag tagtgcgcga gcaaaattta agctacaaca
     9781 aggcaaggct tgaccgacaa ttgcatgaag aatctgctta gggttaggcg ttttgcgctg
     9841 cttcgcgatg tacgggccag atatacgcgt t
//
