LOCUS       sequence-212696-       13233 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  For optogenetic clustering of FGFR1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13233)
  AUTHORS   Dine E, Gil AA, Uribe G, Brangwynne CP, Toettcher JE
  TITLE     Protein Phase Separation Provides Long-Term Memory of Transient 
            Spatial Stimuli.
  JOURNAL   Cell Syst. 2018 May 24. pii: S2405-4712(18)30190-X. doi: 
            10.1016/j.cels.2018.05.002.
  PUBMED    29859829
REFERENCE   2  (bases 1 to 13233)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Syst. 
            2018 May 24. pii: S2405-4712(18)30190-X. doi: 
            10.1016/j.cels.2018.05.002."
COMMENT     Sequence Label: FGFR1-optoDroplet
FEATURES             Location/Qualifiers
     source          1..13233
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             1..634
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    681..806
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1303..1536
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1721..1765
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2050..2167
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2320..2727
                     /label=SFFV promoter
                     /note="spleen focus-forming virus long terminal repeat 
                     (LTR) promoter"
     CDS             2761..2781
                     /codon_start=1
                     /product="N-myristoylation signal from Src kinase (Pellman 
                     et al., 1985; Kaplan et al., 1988)"
                     /label=myr
                     /translation="MGSSKSK"
     CDS             3186..3197
                     /codon_start=1
                     /product="WELQut protease recognition and cleavage site"
                     /label=WELQut site
                     /translation="WELQ"
     CDS             3457..4152
                     /codon_start=1
                     /product="monomeric red fluorescent protein optimized for 
                     use as a fusion tag (Shemiakina et al., 2012)"
                     /label=FusionRed
                     /note="mammalian codon-optimized"
                     /translation="MVSELIKENMPMKLYMEGTVNNHHFKCTSEGEGKPYEGTQTMRIK
                     VVEGGPLPFAFDILATSFMYGSRTFIKHPPGIPDFFKQSFPEGFTWERVTTYEDGGVLT
                     ATQDTSLQDGCLIYNVKVRGVNFPANGPVMQKKTLGWEASTETMYPADGGLEGACDMAL
                     KLVGGGHLICNLETTYRSKKPATNLKMPGVYNVDHRLERIKEADDETYVEQHEVAVARY
                     STGGAGDGGK"
     CDS             4171..5664
                     /codon_start=1
                     /product="N-terminal photolyase homology region of 
                     Arabidopsis thaliana cryptochrome 2"
                     /label=CRY2PHR
                     /note="binds to CIB1 upon exposure to blue light (Kennedy 
                     et al., 2010)"
                     /translation="MKMDKKTIVWFRRDLRIEDNPALAAAAHEGSVFPVFIWCPEEEGQ
                     FYPGRASRWWMKQSLAHLSQSLKALGSDLTLIKTHNTISAILDCIRVTGATKVVFNHLY
                     DPVSLVRDHTVKEKLVERGISVQSYNGDLLYEPWEIYCEKGKPFTSFNSYWKKCLDMSI
                     ESVMLPPPWRLMPITAAAEAIWACSIEELGLENEAEKPSNALLTRAWSPGWSNADKLLN
                     EFIEKQLIDYAKNSKKVVGNSTSLLSPYLHFGEISVRHVFQCARMKQIIWARDKNSEGE
                     ESADLFLRGIGLREYSRYICFNFPFTHEQSLLSHLRFFPWDADVDKFKAWRQGRTGYPL
                     VDAGMRELWATGWMHNRIRVIVSSFAVKFLLLPWKWGMKYFWDTLLDADLECDILGWQY
                     ISGSIPDGHELDRLDNPALQGAKYDPEGEYIRQWLPELARLPTEWIHHPWDAPLTVLKA
                     SGVELGTNYAKPIVDIDTARELLAKAISRTREAQIMIGAA"
     misc_feature    7097..7685
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(7150..7170)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(7568..7579)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(7688..7704)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7689..7705)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             7795..8028
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     promoter        complement(8053..8071)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(8054..8071)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(8361..8380)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     complement(8594..8613)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     8713..8735
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(8773..8791)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        8859..8963
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8964..9032,9033..9824)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(9182..9201)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      9995..10583
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     10484..10503
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     10737..10754
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(10824..10844)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        10829..11158
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      11009..11144
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     intron          12292..12357
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             12487..12507
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    12932..13066
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(12969..12988)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     13023..13042
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
ORIGIN
        1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
       61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
      121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
      181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
      241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
      301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
      361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
      421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
      481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
      541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
      601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
      661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
      721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
      781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
      841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
      901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
      961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
     1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
     1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
     1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
     1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
     1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
     1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
     1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
     1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
     1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
     1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
     1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
     1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
     1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
     1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
     1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
     1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
     1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccaaa tggcagtatt
     2041 catccacaat tttaaaagaa aaggggggat tggggggtac agtgcagggg aaagaatagt
     2101 agacataata gcaacagaca tacaaactaa agaattacaa aaacaaatta caaaaattca
     2161 aaattttcgg gtttattaca gggacagcag agatccagtt tggatcgata agcttgatat
     2221 cgaattcctg cagccccgat aaaataaaag attttattta gtctccagaa aaagggggga
     2281 atgaaagacc ccacctgtag gtttggcaag ctagctgcag taacgccatt ttgcaaggca
     2341 tggaaaaata ccaaaccaag aatagagaag ttcagatcaa gggcgggtac atgaaaatag
     2401 ctaacgttgg gccaaacagg atatctgcgg tgagcagttt cggccccggc ccggggccaa
     2461 gaacagatgg tcaccgcagt ttcggccccg gcccgaggcc aagaacagat ggtccccaga
     2521 tatggcccaa ccctcagcag tttcttaaga cccatcagat gtttccaggc tcccccaagg
     2581 acctgaaatg accctgcgcc ttatttgaat taaccaatca gcctgcttct cgcttctgtt
     2641 cgcgcgcttc tgcttcccga gctctataaa agagctcaca acccctcact cggcgcgcca
     2701 gtcctccgac agactgagtc gcccgggggg gatctggagc tctcgagaat tctcacgcgt
     2761 atgggttcct ccaaatccaa gcccaaggac gcatctcagc gacgtcgcgc ctcaaacgat
     2821 tatacccaac aagcaaccca aagctatggg gcctacccca cccagcccgg gcagggctat
     2881 tcccagcaga gcagtcagcc ctacggacag cagagttaca gtggttatag ccagtccacg
     2941 gacacttcag gctatggcca gagcagctat tcttcttatg gccagagcca gaacacaggc
     3001 tatggaactc agtcaactcc ccagggatat ggctcgactg gcggctatgg cagtagccag
     3061 agctcccaat cgtcttacgg gcagcagtcc tcctatcctg gctatggcca gcagccagct
     3121 cccagcagca cctcgggaag ttacggtagc agttctcaga gcagcagcta tgggcagccc
     3181 cagagtggga gctacagcca gcagcctagc tatggtggac agcagcaaag ctatggacag
     3241 cagcaaagct ataatccccc tcagggctat ggacagcaga accagtacaa cagcagcagt
     3301 ggtggtggag gtggaggtgg aggtggaggt aactatggcc aagatcaatc ctccatgagt
     3361 agtggtggtg gcagtggtgg cggttatggc aatcaagacc agagtggtgg aggtggcagc
     3421 ggtggctatg gacagcagga ccgtggaggt ggaggaatgg tgagcgagct gattaaggag
     3481 aacatgccca tgaagctgta catggagggc accgtgaaca accaccactt caagtgcaca
     3541 tccgagggcg aaggcaagcc ctacgagggc acccagacca tgagaatcaa ggtcgtcgag
     3601 ggcggccctc tccccttcgc cttcgacatc ctggctacca gcttcatgta cggcagcaga
     3661 accttcatca agcaccctcc gggcatcccc gacttcttta agcagtcctt ccctgagggc
     3721 ttcacatggg agagagtcac cacatacgaa gacgggggcg tgctgaccgc tacccaggac
     3781 accagcctcc aggacggctg cctcatctac aacgtcaagg ttagaggggt gaacttccca
     3841 gccaacggcc ctgtgatgca gaagaaaaca ctcggctggg aggcctccac cgagacgatg
     3901 taccccgctg acggcggcct ggaaggcgca tgtgacatgg ccctgaagct cgtgggcggg
     3961 ggccacctga tctgcaacct tgagaccaca tacagatcca agaaacccgc tacgaacctc
     4021 aagatgcccg gcgtctacaa cgtggaccac agactggaaa gaatcaagga ggccgacgat
     4081 gagacctacg tcgagcagca cgaggtggct gtggccagat actctactgg tggcgctggt
     4141 gatggaggta aagcggccac gcgtacgcgt atgaagatgg acaaaaagac tatagtttgg
     4201 tttagaagag acctaaggat tgaggataat cctgcattag cagcagctgc tcacgaagga
     4261 tctgtttttc ctgtcttcat ttggtgtcct gaagaagaag gacagtttta tcctggaaga
     4321 gcttcaagat ggtggatgaa acaatcactt gctcacttat ctcaatcctt gaaggctctt
     4381 ggatctgacc tcactttaat caaaacccac aacacgattt cagcgatctt ggattgtatc
     4441 cgcgttaccg gtgctacaaa agtcgtcttt aaccacctct atgatcctgt ttcgttagtt
     4501 cgggaccata ccgtaaagga gaagctggtg gaacgtggga tctctgtgca aagctacaat
     4561 ggagatctat tgtatgaacc gtgggagata tactgcgaaa agggcaaacc ttttacgagt
     4621 ttcaattctt actggaagaa atgcttagat atgtcgattg aatccgttat gcttcctcct
     4681 ccttggcggt tgatgccaat aactgcagcg gctgaagcga tttgggcgtg ttcgattgaa
     4741 gaactagggc tggagaatga ggccgagaaa ccgagcaatg cgttgttaac tagagcttgg
     4801 tctccaggat ggagcaatgc tgataagtta ctaaatgagt tcatcgagaa gcagttgata
     4861 gattatgcaa agaacagcaa gaaagttgtt gggaattcta cttcactact ttctccgtat
     4921 ctccatttcg gggaaataag cgtcagacac gttttccagt gtgcccggat gaaacaaatt
     4981 atatgggcaa gagataagaa cagtgaagga gaagaaagtg cagatctttt tcttagggga
     5041 atcggtttaa gagagtattc tcggtatata tgtttcaact tcccgtttac tcacgagcaa
     5101 tcgttgttga gtcatcttcg gtttttccct tgggatgctg atgttgataa gttcaaggcc
     5161 tggagacaag gcaggaccgg ttatccgttg gtggatgccg gaatgagaga gctttgggct
     5221 accggatgga tgcataacag aataagagtg attgtttcaa gctttgctgt gaagtttctt
     5281 ctccttccat ggaaatgggg aatgaagtat ttctgggata cacttttgga tgctgatttg
     5341 gaatgtgaca tccttggctg gcagtatatc tctgggagta tccccgatgg ccacgagctt
     5401 gatcgcttgg acaatcccgc gttacaaggc gccaaatatg acccagaagg tgagtacata
     5461 aggcaatggc ttcccgagct tgcgagattg ccaactgaat ggatccatca tccatgggac
     5521 gctcctttaa ccgtactcaa agcttctggt gtggaactcg gaacaaacta tgcgaaaccc
     5581 attgtagaca tcgacacagc tcgtgagcta ctagctaaag ctatttcaag aacccgtgaa
     5641 gcacagatca tgatcggagc agcagcccgg gatccaccgg tcgccaccgg ttcagggagc
     5701 ggatccggct cagggagtgc ggccgacaag atgaagagtg gtaccaagaa gagtgacttc
     5761 cacagccaga tggctgtgca caagctggcc aagagcatcc ctctgcgcag acaggtgtct
     5821 gctgactcca gtgcatccat gaactctggg gttcttctgg ttcggccatc acggctctcc
     5881 tccagtggga ctcccatgct agcaggggtc tctgagtatg agcttcccga agaccctcgc
     5941 tgggagctgc ctcgggacag actggtctta ggcaaacccc tgggagaggg ctgctttggg
     6001 caggtggtgt tggcagaggc tatcgggctg gacaaggaca aacccaaccg tgtgaccaaa
     6061 gtggctgtga agatgttgaa gtcggacgca acagagaaag acttgtcaga cctgatctca
     6121 gaaatggaga tgatgaagat gatcgggaag cataagaata tcatcaacct gctgggggcc
     6181 tgcacgcagg atggtccctt gtatgtcatc gtggagtatg cctccaaggg caacctgcgg
     6241 gagtacctgc aggcccggag gcccccaggg ctggaatact gctacaaccc cagccacaac
     6301 ccagaggagc agctctcctc caaggacctg gtgtcctgcg cctaccaggt ggcccgaggc
     6361 atggagtatc tggcctccaa gaagtgcata caccgagacc tggcagccag gaatgtcctg
     6421 gtgacagagg acaatgtgat gaagatagca gactttggcc tcgcacggga cattcaccac
     6481 atcgactact ataaaaagac aaccaacggc cgactgcctg tgaagtggat ggcacccgag
     6541 gcattatttg accggatcta cacccaccag agtgatgtgt ggtctttcgg ggtgctcctg
     6601 tgggagatct tcactctggg cggctcccca taccccggtg tgcctgtgga ggaacttttc
     6661 aagctgctga aggagggtca ccgcatggac aagcccagta actgcaccaa cgagctgtac
     6721 atgatgatgc gggactgctg gcatgcagtg ccctcacaga gacccacctt caagcagctg
     6781 gtggaagacc tggaccgcat cgtggccttg acctccaacc aggagtacct ggacctgtcc
     6841 atgcccctgg accagtactc ccccagcttt cccgacaccc ggagctctac gtgctcctca
     6901 ggggaggatt ccgtcttctc tcatgagccg ctgcccgagg agccctgcct gccccgacac
     6961 ccagcccagc ttgccaatgg cggactcaaa cgccgctgag cggccgcaac tcccacctgc
     7021 aacatgcgtg actgactgag gccgcgactc tagagtcgac ctgcaggcat gcaagcttga
     7081 tatcaagctt atcgataatc aacctctgga ttacaaaatt tgtgaaagat tgactggtat
     7141 tcttaactat gttgctcctt ttacgctatg tggatacgct gctttaatgc ctttgtatca
     7201 tgctattgct tcccgtatgg ctttcatttt ctcctccttg tataaatcct ggttgctgtc
     7261 tctttatgag gagttgtggc ccgttgtcag gcaacgtggc gtggtgtgca ctgtgtttgc
     7321 tgacgcaacc cccactggtt ggggcattgc caccacctgt cagctccttt ccgggacttt
     7381 cgctttcccc ctccctattg ccacggcgga actcatcgcc gcctgccttg cccgctgctg
     7441 gacaggggct cggctgttgg gcactgacaa ttccgtggtg ttgtcgggga aatcatcgtc
     7501 ctttccttgg ctgctcgcct gtgttgccac ctggattctg cgcgggacgt ccttctgcta
     7561 cgtcccttcg gccctcaatc cagcggacct tccttcccgc ggcctgctgc cggctctgcg
     7621 gcctcttccg cgtcttcgcc ttcgccctca gacgagtcgg atctcccttt gggccgcctc
     7681 cccgcatcga taccgtcgac ctcgagggaa ttaattcgag ctcggtacct ttaagaccaa
     7741 tgacttacaa ggcagctgta gatcttagcc actttttaaa agaaaagggg ggactggaag
     7801 ggctaattca ctcccaacga agacaagatc tgctttttgc ttgtactggg tctctctggt
     7861 tagaccagat ctgagcctgg gagctctctg gctaactagg gaacccactg cttaagcctc
     7921 aataaagctt gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt gactctggta
     7981 actagagatc cctcagaccc ttttagtcag tgtggaaaat ctctagcagc atctagaatt
     8041 aattccgtgt attctatagt gtcacctaaa tcgtatgtgt atgatacata aggttatgta
     8101 ttaattgtag ccgcgttcta acgacaatat gtacaagcct aattgtgtag catctggctt
     8161 actgaagcag accctatcat ctctctcgta aactgccgtc agagtcggtt tggttggacg
     8221 aaccttctga gtttctggta acgccgtccc gcacccggaa atggtcagcg aaccaatcag
     8281 cagggtcatc gctagccaga tcctctacgc cggacgcatc gtggccggca tcaccggcgc
     8341 cacaggtgcg gttgctggcg cctatatcgc cgacatcacc gatggggaag atcgggctcg
     8401 ccacttcggg ctcatgagcg cttgtttcgg cgtgggtatg gtggcaggcc ccgtggccgg
     8461 gggactgttg ggcgccatct ccttgcatgc accattcctt gcggcggcgg tgctcaacgg
     8521 cctcaaccta ctactgggct gcttcctaat gcaggagtcg cataagggag agcgtcgaat
     8581 ggtgcactct cagtacaatc tgctctgatg ccgcatagtt aagccagccc cgacacccgc
     8641 caacacccgc tgacgcgccc tgacgggctt gtctgctccc ggcatccgct tacagacaag
     8701 ctgtgaccgt ctccgggagc tgcatgtgtc agaggttttc accgtcatca ccgaaacgcg
     8761 cgagacgaaa gggcctcgtg atacgcctat ttttataggt taatgtcatg ataataatgg
     8821 tttcttagac gtcaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat
     8881 ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc
     8941 aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct
     9001 tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag
     9061 atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta
     9121 agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact tttaaagttc
     9181 tgctatgtgg cgcggtatta tcccgtattg acgccgggca agagcaactc ggtcgccgca
     9241 tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag catcttacgg
     9301 atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat aacactgcgg
     9361 ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt ttgcacaaca
     9421 tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa
     9481 acgacgagcg tgacaccacg atgcctgtag caatggcaac aacgttgcgc aaactattaa
     9541 ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg gaggcggata
     9601 aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat
     9661 ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc
     9721 cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata
     9781 gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt
     9841 actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga
     9901 agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag
     9961 cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa
    10021 tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag
    10081 agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg
    10141 ttcttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat
    10201 acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta
    10261 ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg
    10321 gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc
    10381 gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa
    10441 gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc
    10501 tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt
    10561 caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct
    10621 tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc
    10681 gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg
    10741 agtcagtgag cgaggaagcg gaagagcgcc caatacgcaa accgcctctc cccgcgcgtt
    10801 ggccgattca ttaatgcagc tgtggaatgt gtgtcagtta gggtgtggaa agtccccagg
    10861 ctccccagca ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa ccaggtgtgg
    10921 aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcatctca attagtcagc
    10981 aaccatagtc ccgcccctaa ctccgcccat cccgccccta actccgccca gttccgccca
    11041 ttctccgccc catggctgac taattttttt tatttatgca gaggccgagg ccgcctcggc
    11101 ctctgagcta ttccagaagt agtgaggagg cttttttgga ggcctaggct tttgcaaaaa
    11161 gcttggacac aagacaggct tgcgagatat gtttgagaat accactttat cccgcgtcag
    11221 ggagaggcag tgcgtaaaaa gacgcggact catgtgaaat actggttttt agtgcgccag
    11281 atctctataa tctcgcgcaa cctattttcc cctcgaacac tttttaagcc gtagataaac
    11341 aggctgggac acttcacatg agcgaaaaat acatcgtcac ctgggacatg ttgcagatcc
    11401 atgcacgtaa actcgcaagc cgactgatgc cttctgaaca atggaaaggc attattgccg
    11461 taagccgtgg cggtctgtac cgggtgcgtt actggcgcgt gaactgggta ttcgtcatgt
    11521 cgataccgtt tgtatttcca gctacgatca cgacaaccag cgcgagctta aagtgctgaa
    11581 acgcgcagaa ggcgatggcg aaggcttcat cgttattgat gacctggtgg ataccggtgg
    11641 tactgcggtt gcgattcgtg aaatgtatcc aaaagcgcac tttgtcacca tcttcgcaaa
    11701 accggctggt cgtccgctgg ttgatgacta tgttgttgat atcccgcaag atacctggat
    11761 tgaacagccg tgggatatgg gcgtcgtatt cgtcccgcca atctccggtc gctaatcttt
    11821 tcaacgcctg gcactgccgg gcgttgttct ttttaacttc aggcgggtta caatagtttc
    11881 cagtaagtat tctggaggct gcatccatga cacaggcaaa cctgagcgaa accctgttca
    11941 aaccccgctt taaacatcct gaaacctcga cgctagtccg ccgctttaat cacggcgcac
    12001 aaccgcctgt gcagtcggcc cttgatggta aaaccatccc tcactggtat cgcatgatta
    12061 accgtctgat gtggatctgg cgcggcattg acccacgcga aatcctcgac gtccaggcac
    12121 gtattgtgat gagcgatgcc gaacgtaccg acgatgattt atacgatacg gtgattggct
    12181 accgtggcgg caactggatt tatgagtggg ccccggatct ttgtgaagga accttacttc
    12241 tgtggtgtga cataattgga caaactacct acagagattt aaagctctaa ggtaaatata
    12301 aaatttttaa gtgtataatg tgttaaacta ctgattctaa ttgtttgtgt attttagatt
    12361 ccaacctatg gaactgatga atgggagcag tggtggaatg cctttaatga ggaaaacctg
    12421 ttttgctcag aagaaatgcc atctagtgat gatgaggcta ctgctgactc tcaacattct
    12481 actcctccaa aaaagaagag aaaggtagaa gaccccaagg actttccttc agaattgcta
    12541 agttttttga gtcatgctgt gtttagtaat agaactcttg cttgctttgc tatttacacc
    12601 acaaaggaaa aagctgcact gctatacaag aaaattatgg aaaaatattc tgtaaccttt
    12661 ataagtaggc ataacagtta taatcataac atactgtttt ttcttactcc acacaggcat
    12721 agagtgtctg ctattaataa ctatgctcaa aaattgtgta cctttagctt tttaatttgt
    12781 aaaggggtta ataaggaata tttgatgtat agtgccttga ctagagatca taatcagcca
    12841 taccacattt gtagaggttt tacttgcttt aaaaaacctc ccacacctcc ccctgaacct
    12901 gaaacataaa atgaatgcaa ttgttgttgt taacttgttt attgcagctt ataatggtta
    12961 caaataaagc aatagcatca caaatttcac aaataaagca tttttttcac tgcattctag
    13021 ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc tggatcaact ggataactca
    13081 agctaaccaa aatcatccca aacttcccac cccataccct attaccactg ccaattacct
    13141 gtggtttcat ttactctaaa cctgtgattc ctctgaatta ttttcatttt aaagaaattg
    13201 tatttgttaa atatgtacta caaacttagt agt
//
