LOCUS       sequence-212705-       10838 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  For PixELL system.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10838)
  AUTHORS   Dine E, Gil AA, Uribe G, Brangwynne CP, Toettcher JE
  TITLE     Protein Phase Separation Provides Long-Term Memory of Transient 
            Spatial Stimuli.
  JOURNAL   Cell Syst. 2018 May 24. pii: S2405-4712(18)30190-X. doi: 
            10.1016/j.cels.2018.05.002.
  PUBMED    29859829
REFERENCE   2  (bases 1 to 10838)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Syst. 
            2018 May 24. pii: S2405-4712(18)30190-X. doi: 
            10.1016/j.cels.2018.05.002."
COMMENT     Sequence Label: FUS-FusionRed-PixD
FEATURES             Location/Qualifiers
     source          1..10838
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             1..634
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    681..806
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1303..1536
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1721..1765
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2050..2167
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2320..2727
                     /label=SFFV promoter
                     /note="spleen focus-forming virus long terminal repeat 
                     (LTR) promoter"
     CDS             3173..3184
                     /codon_start=1
                     /product="WELQut protease recognition and cleavage site"
                     /label=WELQut site
                     /translation="WELQ"
     CDS             3444..4139
                     /codon_start=1
                     /product="monomeric red fluorescent protein optimized for 
                     use as a fusion tag (Shemiakina et al., 2012)"
                     /label=FusionRed
                     /note="mammalian codon-optimized"
                     /translation="MVSELIKENMPMKLYMEGTVNNHHFKCTSEGEGKPYEGTQTMRIK
                     VVEGGPLPFAFDILATSFMYGSRTFIKHPPGIPDFFKQSFPEGFTWERVTTYEDGGVLT
                     ATQDTSLQDGCLIYNVKVRGVNFPANGPVMQKKTLGWEASTETMYPADGGLEGACDMAL
                     KLVGGGHLICNLETTYRSKKPATNLKMPGVYNVDHRLERIKEADDETYVEQHEVAVARY
                     STGGAGDGGK"
     misc_feature    4702..5290
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(4755..4775)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(5173..5184)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(5293..5309)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5294..5310)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             5400..5633
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     promoter        complement(5658..5676)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(5659..5676)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(5966..5985)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     complement(6199..6218)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     6318..6340
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(6378..6396)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        6464..6568
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6569..6637,6638..7429)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6787..6806)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7600..8188
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8089..8108
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8342..8359
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(8429..8449)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        8434..8763
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      8614..8749
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     intron          9897..9962
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             10092..10112
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    10537..10671
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(10574..10593)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     10628..10647
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
ORIGIN
        1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
       61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
      121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
      181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
      241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
      301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
      361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
      421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
      481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
      541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
      601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
      661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
      721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
      781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
      841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
      901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
      961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
     1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
     1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
     1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
     1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
     1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
     1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
     1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
     1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
     1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
     1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
     1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
     1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
     1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
     1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
     1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
     1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
     1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccaaa tggcagtatt
     2041 catccacaat tttaaaagaa aaggggggat tggggggtac agtgcagggg aaagaatagt
     2101 agacataata gcaacagaca tacaaactaa agaattacaa aaacaaatta caaaaattca
     2161 aaattttcgg gtttattaca gggacagcag agatccagtt tggatcgata agcttgatat
     2221 cgaattcctg cagccccgat aaaataaaag attttattta gtctccagaa aaagggggga
     2281 atgaaagacc ccacctgtag gtttggcaag ctagctgcag taacgccatt ttgcaaggca
     2341 tggaaaaata ccaaaccaag aatagagaag ttcagatcaa gggcgggtac atgaaaatag
     2401 ctaacgttgg gccaaacagg atatctgcgg tgagcagttt cggccccggc ccggggccaa
     2461 gaacagatgg tcaccgcagt ttcggccccg gcccgaggcc aagaacagat ggtccccaga
     2521 tatggcccaa ccctcagcag tttcttaaga cccatcagat gtttccaggc tcccccaagg
     2581 acctgaaatg accctgcgcc ttatttgaat taaccaatca gcctgcttct cgcttctgtt
     2641 cgcgcgcttc tgcttcccga gctctataaa agagctcaca acccctcact cggcgcgcca
     2701 gtcctccgac agactgagtc gcccgggggg gatctggagc tctcgagaat tctcacgcgt
     2761 caagtggagc aaggcaggtg gacagtggat ccatggcctc aaacgattat acccaacaag
     2821 caacccaaag ctatggggcc taccccaccc agcccgggca gggctattcc cagcagagca
     2881 gtcagcccta cggacagcag agttacagtg gttatagcca gtccacggac acttcaggct
     2941 atggccagag cagctattct tcttatggcc agagccagaa cacaggctat ggaactcagt
     3001 caactcccca gggatatggc tcgactggcg gctatggcag tagccagagc tcccaatcgt
     3061 cttacgggca gcagtcctcc tatcctggct atggccagca gccagctccc agcagcacct
     3121 cgggaagtta cggtagcagt tctcagagca gcagctatgg gcagccccag agtgggagct
     3181 acagccagca gcctagctat ggtggacagc agcaaagcta tggacagcag caaagctata
     3241 atccccctca gggctatgga cagcagaacc agtacaacag cagcagtggt ggtggaggtg
     3301 gaggtggagg tggaggtaac tatggccaag atcaatcctc catgagtagt ggtggtggca
     3361 gtggtggcgg ttatggcaat caagaccaga gtggtggagg tggcagcggt ggctatggac
     3421 agcaggaccg tggaggtgga ggaatggtga gcgagctgat taaggagaac atgcccatga
     3481 agctgtacat ggagggcacc gtgaacaacc accacttcaa gtgcacatcc gagggcgaag
     3541 gcaagcccta cgagggcacc cagaccatga gaatcaaggt cgtcgagggc ggccctctcc
     3601 ccttcgcctt cgacatcctg gctaccagct tcatgtacgg cagcagaacc ttcatcaagc
     3661 accctccggg catccccgac ttctttaagc agtccttccc tgagggcttc acatgggaga
     3721 gagtcaccac atacgaagac gggggcgtgc tgaccgctac ccaggacacc agcctccagg
     3781 acggctgcct catctacaac gtcaaggtta gaggggtgaa cttcccagcc aacggccctg
     3841 tgatgcagaa gaaaacactc ggctgggagg cctccaccga gacgatgtac cccgctgacg
     3901 gcggcctgga aggcgcatgt gacatggccc tgaagctcgt gggcgggggc cacctgatct
     3961 gcaaccttga gaccacatac agatccaaga aacccgctac gaacctcaag atgcccggcg
     4021 tctacaacgt ggaccacaga ctggaaagaa tcaaggaggc cgacgatgag acctacgtcg
     4081 agcagcacga ggtggctgtg gccagatact ctactggtgg cgctggtgat ggaggtaaag
     4141 cggccacgcg tatgagtttg taccgtttga tttacagcag tcagggcatt cccaatttac
     4201 aaccccagga tttaaaagat atcttagaat cttcccaaag aaataatccg gccaatggca
     4261 ttaccggact gctctgctac agcaagccgg cttttctgca ggtattggaa ggagagtgtg
     4321 aacaggttaa tgaaacctac caccggattg tgcaagatga acgccaccac agcccccaga
     4381 tcattgagtg tatgccgatc agaagaagaa acttcgaggt ttggtctatg caagcgatca
     4441 cggtgaatga tttgtcaacg gaacaggtta agactttggt attgaaatat tccggcttca
     4501 ccaccctacg gcccagtgcc atggacccgg aacaatgcct aaactttttg ctagacattg
     4561 ccaagattta cgagttgtcc gataactttt tcctcgacct ctaagcggcc gcaactccca
     4621 cctgcaacat gcgtgactga ctgaggccgc gactctagag tcgacctgca ggcatgcaag
     4681 cttgatatca agcttatcga taatcaacct ctggattaca aaatttgtga aagattgact
     4741 ggtattctta actatgttgc tccttttacg ctatgtggat acgctgcttt aatgcctttg
     4801 tatcatgcta ttgcttcccg tatggctttc attttctcct ccttgtataa atcctggttg
     4861 ctgtctcttt atgaggagtt gtggcccgtt gtcaggcaac gtggcgtggt gtgcactgtg
     4921 tttgctgacg caacccccac tggttggggc attgccacca cctgtcagct cctttccggg
     4981 actttcgctt tccccctccc tattgccacg gcggaactca tcgccgcctg ccttgcccgc
     5041 tgctggacag gggctcggct gttgggcact gacaattccg tggtgttgtc ggggaaatca
     5101 tcgtcctttc cttggctgct cgcctgtgtt gccacctgga ttctgcgcgg gacgtccttc
     5161 tgctacgtcc cttcggccct caatccagcg gaccttcctt cccgcggcct gctgccggct
     5221 ctgcggcctc ttccgcgtct tcgccttcgc cctcagacga gtcggatctc cctttgggcc
     5281 gcctccccgc atcgataccg tcgacctcga gggaattaat tcgagctcgg tacctttaag
     5341 accaatgact tacaaggcag ctgtagatct tagccacttt ttaaaagaaa aggggggact
     5401 ggaagggcta attcactccc aacgaagaca agatctgctt tttgcttgta ctgggtctct
     5461 ctggttagac cagatctgag cctgggagct ctctggctaa ctagggaacc cactgcttaa
     5521 gcctcaataa agcttgcctt gagtgcttca agtagtgtgt gcccgtctgt tgtgtgactc
     5581 tggtaactag agatccctca gaccctttta gtcagtgtgg aaaatctcta gcagcatcta
     5641 gaattaattc cgtgtattct atagtgtcac ctaaatcgta tgtgtatgat acataaggtt
     5701 atgtattaat tgtagccgcg ttctaacgac aatatgtaca agcctaattg tgtagcatct
     5761 ggcttactga agcagaccct atcatctctc tcgtaaactg ccgtcagagt cggtttggtt
     5821 ggacgaacct tctgagtttc tggtaacgcc gtcccgcacc cggaaatggt cagcgaacca
     5881 atcagcaggg tcatcgctag ccagatcctc tacgccggac gcatcgtggc cggcatcacc
     5941 ggcgccacag gtgcggttgc tggcgcctat atcgccgaca tcaccgatgg ggaagatcgg
     6001 gctcgccact tcgggctcat gagcgcttgt ttcggcgtgg gtatggtggc aggccccgtg
     6061 gccgggggac tgttgggcgc catctccttg catgcaccat tccttgcggc ggcggtgctc
     6121 aacggcctca acctactact gggctgcttc ctaatgcagg agtcgcataa gggagagcgt
     6181 cgaatggtgc actctcagta caatctgctc tgatgccgca tagttaagcc agccccgaca
     6241 cccgccaaca cccgctgacg cgccctgacg ggcttgtctg ctcccggcat ccgcttacag
     6301 acaagctgtg accgtctccg ggagctgcat gtgtcagagg ttttcaccgt catcaccgaa
     6361 acgcgcgaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat
     6421 aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg
     6481 tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat
     6541 gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat
     6601 tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt
     6661 aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag
     6721 cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa
     6781 agttctgcta tgtggcgcgg tattatcccg tattgacgcc gggcaagagc aactcggtcg
     6841 ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct
     6901 tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac
     6961 tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca
     7021 caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat
     7081 accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact
     7141 attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc
     7201 ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga
     7261 taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg
     7321 taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg
     7381 aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca
     7441 agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta
     7501 ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca
     7561 ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg
     7621 cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga
     7681 tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa
     7741 tactgttctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc
     7801 tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg
     7861 tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac
     7921 ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct
     7981 acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc
     8041 ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg
     8101 gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg
     8161 ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct
     8221 ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga
     8281 taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg
     8341 cagcgagtca gtgagcgagg aagcggaaga gcgcccaata cgcaaaccgc ctctccccgc
     8401 gcgttggccg attcattaat gcagctgtgg aatgtgtgtc agttagggtg tggaaagtcc
     8461 ccaggctccc cagcaggcag aagtatgcaa agcatgcatc tcaattagtc agcaaccagg
     8521 tgtggaaagt ccccaggctc cccagcaggc agaagtatgc aaagcatgca tctcaattag
     8581 tcagcaacca tagtcccgcc cctaactccg cccatcccgc ccctaactcc gcccagttcc
     8641 gcccattctc cgccccatgg ctgactaatt ttttttattt atgcagaggc cgaggccgcc
     8701 tcggcctctg agctattcca gaagtagtga ggaggctttt ttggaggcct aggcttttgc
     8761 aaaaagcttg gacacaagac aggcttgcga gatatgtttg agaataccac tttatcccgc
     8821 gtcagggaga ggcagtgcgt aaaaagacgc ggactcatgt gaaatactgg tttttagtgc
     8881 gccagatctc tataatctcg cgcaacctat tttcccctcg aacacttttt aagccgtaga
     8941 taaacaggct gggacacttc acatgagcga aaaatacatc gtcacctggg acatgttgca
     9001 gatccatgca cgtaaactcg caagccgact gatgccttct gaacaatgga aaggcattat
     9061 tgccgtaagc cgtggcggtc tgtaccgggt gcgttactgg cgcgtgaact gggtattcgt
     9121 catgtcgata ccgtttgtat ttccagctac gatcacgaca accagcgcga gcttaaagtg
     9181 ctgaaacgcg cagaaggcga tggcgaaggc ttcatcgtta ttgatgacct ggtggatacc
     9241 ggtggtactg cggttgcgat tcgtgaaatg tatccaaaag cgcactttgt caccatcttc
     9301 gcaaaaccgg ctggtcgtcc gctggttgat gactatgttg ttgatatccc gcaagatacc
     9361 tggattgaac agccgtggga tatgggcgtc gtattcgtcc cgccaatctc cggtcgctaa
     9421 tcttttcaac gcctggcact gccgggcgtt gttcttttta acttcaggcg ggttacaata
     9481 gtttccagta agtattctgg aggctgcatc catgacacag gcaaacctga gcgaaaccct
     9541 gttcaaaccc cgctttaaac atcctgaaac ctcgacgcta gtccgccgct ttaatcacgg
     9601 cgcacaaccg cctgtgcagt cggcccttga tggtaaaacc atccctcact ggtatcgcat
     9661 gattaaccgt ctgatgtgga tctggcgcgg cattgaccca cgcgaaatcc tcgacgtcca
     9721 ggcacgtatt gtgatgagcg atgccgaacg taccgacgat gatttatacg atacggtgat
     9781 tggctaccgt ggcggcaact ggatttatga gtgggccccg gatctttgtg aaggaacctt
     9841 acttctgtgg tgtgacataa ttggacaaac tacctacaga gatttaaagc tctaaggtaa
     9901 atataaaatt tttaagtgta taatgtgtta aactactgat tctaattgtt tgtgtatttt
     9961 agattccaac ctatggaact gatgaatggg agcagtggtg gaatgccttt aatgaggaaa
    10021 acctgttttg ctcagaagaa atgccatcta gtgatgatga ggctactgct gactctcaac
    10081 attctactcc tccaaaaaag aagagaaagg tagaagaccc caaggacttt ccttcagaat
    10141 tgctaagttt tttgagtcat gctgtgttta gtaatagaac tcttgcttgc tttgctattt
    10201 acaccacaaa ggaaaaagct gcactgctat acaagaaaat tatggaaaaa tattctgtaa
    10261 cctttataag taggcataac agttataatc ataacatact gttttttctt actccacaca
    10321 ggcatagagt gtctgctatt aataactatg ctcaaaaatt gtgtaccttt agctttttaa
    10381 tttgtaaagg ggttaataag gaatatttga tgtatagtgc cttgactaga gatcataatc
    10441 agccatacca catttgtaga ggttttactt gctttaaaaa acctcccaca cctccccctg
    10501 aacctgaaac ataaaatgaa tgcaattgtt gttgttaact tgtttattgc agcttataat
    10561 ggttacaaat aaagcaatag catcacaaat ttcacaaata aagcattttt ttcactgcat
    10621 tctagttgtg gtttgtccaa actcatcaat gtatcttatc atgtctggat caactggata
    10681 actcaagcta accaaaatca tcccaaactt cccaccccat accctattac cactgccaat
    10741 tacctgtggt ttcatttact ctaaacctgt gattcctctg aattattttc attttaaaga
    10801 aattgtattt gttaaatatg tactacaaac ttagtagt
//
