LOCUS       sequence-212707-       11558 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  For PixELL system.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11558)
  AUTHORS   Dine E, Gil AA, Uribe G, Brangwynne CP, Toettcher JE
  TITLE     Protein Phase Separation Provides Long-Term Memory of Transient 
            Spatial Stimuli.
  JOURNAL   Cell Syst. 2018 May 24. pii: S2405-4712(18)30190-X. doi: 
            10.1016/j.cels.2018.05.002.
  PUBMED    29859829
REFERENCE   2  (bases 1 to 11558)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Syst. 
            2018 May 24. pii: S2405-4712(18)30190-X. doi: 
            10.1016/j.cels.2018.05.002."
COMMENT     Sequence Label: FUS-Citrine-PixE
FEATURES             Location/Qualifiers
     source          1..11558
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             1..634
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    681..806
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1303..1536
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1721..1765
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2050..2167
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2320..2727
                     /label=SFFV promoter
                     /note="spleen focus-forming virus long terminal repeat 
                     (LTR) promoter"
     CDS             3182..3193
                     /codon_start=1
                     /product="WELQut protease recognition and cleavage site"
                     /label=WELQut site
                     /translation="WELQ"
     CDS             join(3453..3455,3456..3458,3459..4169)
                     /codon_start=1
                     /product="enhanced variant of YFP (Heikal et al., 2001)"
                     /label=Citrine
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTFGYGLMCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(3498..3519)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(3759..3778)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     4106..4127
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     misc_feature    5422..6010
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(5475..5495)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(5893..5904)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(6013..6029)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6014..6030)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             6120..6353
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     promoter        complement(6378..6396)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(6379..6396)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(6686..6705)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     complement(6919..6938)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     7038..7060
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(7098..7116)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        7184..7288
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7289..7357,7358..8149)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7507..7526)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8320..8908
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8809..8828
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     9062..9079
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(9149..9169)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        9154..9483
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      9334..9469
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     intron          10617..10682
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             10812..10832
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    11257..11391
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(11294..11313)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     11348..11367
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
ORIGIN
        1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
       61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
      121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
      181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
      241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
      301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
      361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
      421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
      481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
      541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
      601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
      661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
      721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
      781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
      841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
      901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
      961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
     1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
     1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
     1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
     1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
     1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
     1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
     1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
     1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
     1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
     1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
     1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
     1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
     1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
     1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
     1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
     1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
     1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccaaa tggcagtatt
     2041 catccacaat tttaaaagaa aaggggggat tggggggtac agtgcagggg aaagaatagt
     2101 agacataata gcaacagaca tacaaactaa agaattacaa aaacaaatta caaaaattca
     2161 aaattttcgg gtttattaca gggacagcag agatccagtt tggatcgata agcttgatat
     2221 cgaattcctg cagccccgat aaaataaaag attttattta gtctccagaa aaagggggga
     2281 atgaaagacc ccacctgtag gtttggcaag ctagctgcag taacgccatt ttgcaaggca
     2341 tggaaaaata ccaaaccaag aatagagaag ttcagatcaa gggcgggtac atgaaaatag
     2401 ctaacgttgg gccaaacagg atatctgcgg tgagcagttt cggccccggc ccggggccaa
     2461 gaacagatgg tcaccgcagt ttcggccccg gcccgaggcc aagaacagat ggtccccaga
     2521 tatggcccaa ccctcagcag tttcttaaga cccatcagat gtttccaggc tcccccaagg
     2581 acctgaaatg accctgcgcc ttatttgaat taaccaatca gcctgcttct cgcttctgtt
     2641 cgcgcgcttc tgcttcccga gctctataaa agagctcaca acccctcact cggcgcgcca
     2701 gtcctccgac agactgagtc gcccgggggg gatctggagc tctcgagaat tctcacgcgt
     2761 caagtggagc aaggcaggtg gacagtggat ccggagctac catggcctca aacgattata
     2821 cccaacaagc aacccaaagc tatggggcct accccaccca gcccgggcag ggctattccc
     2881 agcagagcag tcagccctac ggacagcaga gttacagtgg ttatagccag tccacggaca
     2941 cttcaggcta tggccagagc agctattctt cttatggcca gagccagaac acaggctatg
     3001 gaactcagtc aactccccag ggatatggct cgactggcgg ctatggcagt agccagagct
     3061 cccaatcgtc ttacgggcag cagtcctcct atcctggcta tggccagcag ccagctccca
     3121 gcagcacctc gggaagttac ggtagcagtt ctcagagcag cagctatggg cagccccaga
     3181 gtgggagcta cagccagcag cctagctatg gtggacagca gcaaagctat ggacagcagc
     3241 aaagctataa tccccctcag ggctatggac agcagaacca gtacaacagc agcagtggtg
     3301 gtggaggtgg aggtggaggt ggaggtaact atggccaaga tcaatcctcc atgagtagtg
     3361 gtggtggcag tggtggcggt tatggcaatc aagaccagag tggtggaggt ggcagcggtg
     3421 gctatggaca gcaggaccgt ggaggtggag gaatggtgag caagggcgag gagctgttca
     3481 ccggggtggt gcccatcctg gtcgagctgg acggcgacgt aaacggccac aagttcagcg
     3541 tgtccggcga gggcgagggc gatgccacct acggcaagct gaccctgaag ttcatctgca
     3601 ccaccggcaa gctgcccgtg ccctggccca ccctcgtgac caccttcggc tacggcctga
     3661 tgtgcttcgc ccgctacccc gaccacatga agcagcacga cttcttcaag tccgccatgc
     3721 ccgaaggcta cgtccaggag cgcaccatct tcttcaagga cgacggcaac tacaagaccc
     3781 gcgccgaggt gaagttcgag ggcgacaccc tggtgaaccg catcgagctg aagggcatcg
     3841 acttcaagga ggacggcaac atcctggggc acaagctgga gtacaactac aacagccaca
     3901 acgtctatat catggccgac aagcagaaga acggcatcaa ggtgaacttc aagatccgcc
     3961 acaacatcga ggacggcagc gtgcagctcg ccgaccacta ccagcagaac acccccatcg
     4021 gcgacggccc cgtgctgctg cccgacaacc actacctgag ctaccagtcc gccctgagca
     4081 aagaccccaa cgagaagcgc gatcacatgg tcctgctgga gttcgtgacc gccgccggga
     4141 tcactctcgg catggacgag ctgtacaagg cggccacgcg tatgagcaat tcagttttgt
     4201 ccacttcttc cccaggtaat atccaaactc ggcaggcgat tccggcccaa ctcctctatt
     4261 ccctcgtcaa ccaaaaagcc agcggcaaac ttgtggtaca aaaccccttt gacgaatttg
     4321 tttattggca agtttatttg ggggatggtc gtattcactt ggccaacagc gccaatggtt
     4381 gttccgaaag acttaattat ctgattggta gcacccttcg tcagaagggc attgaactgc
     4441 catcaagaat tgctgacgat tatatttttc tttccgggct ctggaaaagg gatgtttttt
     4501 cctttcagca aacccgctcg attctgaccc aagctagcca agaagcctta gttcaggtac
     4561 tttccttacc taaaacatcc tatcgtttta atcaagaaaa agaactgagc gaactatttt
     4621 tgaatttaga gttagaaaaa actattaccc ccatcaaaca taaaattcgc tattggtggg
     4681 aattgaggtc ctatattaac tcgcctttcc agcgacccct agtggaaaac tggcttagtc
     4741 tgcgccaaaa tttggtgaaa aataacctcc atggccaaca ttggctaaaa cgcttccaca
     4801 cttgcttgga aaatctaaat tgcttatatg aaattgccgg gcatttggaa atcagtactt
     4861 tggagttggc attaatgcta cagcccatgg tcaagactgg cgaaatcaag atgctgcctt
     4921 atcaagaaat tcaaacggat gaccgccctt tggtggctta tgttgaccat agggaagtcc
     4981 agcagcgcat gatgacctat tctttggaaa aagaaggcta tcgagcctta gtactggatg
     5041 atccctttcg ggctttaacg gtgttgttga attctccccc caatctgatt atcataaata
     5101 ctgacttgcc ggacatgaac ggttatcaac tctgttcgtt ttgccgtaag tccaatcaac
     5161 ttaaagatgt gccgatccta ctattgggga aaggtgacaa tttgatcagc ggtattcgta
     5221 tcaaactttg tcaagcttct gggtacttaa gtgagccttt tgttccagaa gaattactcg
     5281 atcgggtggc aaaactcctt cccaccaata aaaccaactc ctgagcggcc gcaactccca
     5341 cctgcaacat gcgtgactga ctgaggccgc gactctagag tcgacctgca ggcatgcaag
     5401 cttgatatca agcttatcga taatcaacct ctggattaca aaatttgtga aagattgact
     5461 ggtattctta actatgttgc tccttttacg ctatgtggat acgctgcttt aatgcctttg
     5521 tatcatgcta ttgcttcccg tatggctttc attttctcct ccttgtataa atcctggttg
     5581 ctgtctcttt atgaggagtt gtggcccgtt gtcaggcaac gtggcgtggt gtgcactgtg
     5641 tttgctgacg caacccccac tggttggggc attgccacca cctgtcagct cctttccggg
     5701 actttcgctt tccccctccc tattgccacg gcggaactca tcgccgcctg ccttgcccgc
     5761 tgctggacag gggctcggct gttgggcact gacaattccg tggtgttgtc ggggaaatca
     5821 tcgtcctttc cttggctgct cgcctgtgtt gccacctgga ttctgcgcgg gacgtccttc
     5881 tgctacgtcc cttcggccct caatccagcg gaccttcctt cccgcggcct gctgccggct
     5941 ctgcggcctc ttccgcgtct tcgccttcgc cctcagacga gtcggatctc cctttgggcc
     6001 gcctccccgc atcgataccg tcgacctcga gggaattaat tcgagctcgg tacctttaag
     6061 accaatgact tacaaggcag ctgtagatct tagccacttt ttaaaagaaa aggggggact
     6121 ggaagggcta attcactccc aacgaagaca agatctgctt tttgcttgta ctgggtctct
     6181 ctggttagac cagatctgag cctgggagct ctctggctaa ctagggaacc cactgcttaa
     6241 gcctcaataa agcttgcctt gagtgcttca agtagtgtgt gcccgtctgt tgtgtgactc
     6301 tggtaactag agatccctca gaccctttta gtcagtgtgg aaaatctcta gcagcatcta
     6361 gaattaattc cgtgtattct atagtgtcac ctaaatcgta tgtgtatgat acataaggtt
     6421 atgtattaat tgtagccgcg ttctaacgac aatatgtaca agcctaattg tgtagcatct
     6481 ggcttactga agcagaccct atcatctctc tcgtaaactg ccgtcagagt cggtttggtt
     6541 ggacgaacct tctgagtttc tggtaacgcc gtcccgcacc cggaaatggt cagcgaacca
     6601 atcagcaggg tcatcgctag ccagatcctc tacgccggac gcatcgtggc cggcatcacc
     6661 ggcgccacag gtgcggttgc tggcgcctat atcgccgaca tcaccgatgg ggaagatcgg
     6721 gctcgccact tcgggctcat gagcgcttgt ttcggcgtgg gtatggtggc aggccccgtg
     6781 gccgggggac tgttgggcgc catctccttg catgcaccat tccttgcggc ggcggtgctc
     6841 aacggcctca acctactact gggctgcttc ctaatgcagg agtcgcataa gggagagcgt
     6901 cgaatggtgc actctcagta caatctgctc tgatgccgca tagttaagcc agccccgaca
     6961 cccgccaaca cccgctgacg cgccctgacg ggcttgtctg ctcccggcat ccgcttacag
     7021 acaagctgtg accgtctccg ggagctgcat gtgtcagagg ttttcaccgt catcaccgaa
     7081 acgcgcgaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat
     7141 aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg
     7201 tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat
     7261 gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat
     7321 tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt
     7381 aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag
     7441 cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa
     7501 agttctgcta tgtggcgcgg tattatcccg tattgacgcc gggcaagagc aactcggtcg
     7561 ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct
     7621 tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac
     7681 tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca
     7741 caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat
     7801 accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact
     7861 attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc
     7921 ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga
     7981 taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg
     8041 taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg
     8101 aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca
     8161 agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta
     8221 ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca
     8281 ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg
     8341 cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga
     8401 tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa
     8461 tactgttctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc
     8521 tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg
     8581 tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac
     8641 ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct
     8701 acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc
     8761 ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg
     8821 gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg
     8881 ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct
     8941 ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga
     9001 taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg
     9061 cagcgagtca gtgagcgagg aagcggaaga gcgcccaata cgcaaaccgc ctctccccgc
     9121 gcgttggccg attcattaat gcagctgtgg aatgtgtgtc agttagggtg tggaaagtcc
     9181 ccaggctccc cagcaggcag aagtatgcaa agcatgcatc tcaattagtc agcaaccagg
     9241 tgtggaaagt ccccaggctc cccagcaggc agaagtatgc aaagcatgca tctcaattag
     9301 tcagcaacca tagtcccgcc cctaactccg cccatcccgc ccctaactcc gcccagttcc
     9361 gcccattctc cgccccatgg ctgactaatt ttttttattt atgcagaggc cgaggccgcc
     9421 tcggcctctg agctattcca gaagtagtga ggaggctttt ttggaggcct aggcttttgc
     9481 aaaaagcttg gacacaagac aggcttgcga gatatgtttg agaataccac tttatcccgc
     9541 gtcagggaga ggcagtgcgt aaaaagacgc ggactcatgt gaaatactgg tttttagtgc
     9601 gccagatctc tataatctcg cgcaacctat tttcccctcg aacacttttt aagccgtaga
     9661 taaacaggct gggacacttc acatgagcga aaaatacatc gtcacctggg acatgttgca
     9721 gatccatgca cgtaaactcg caagccgact gatgccttct gaacaatgga aaggcattat
     9781 tgccgtaagc cgtggcggtc tgtaccgggt gcgttactgg cgcgtgaact gggtattcgt
     9841 catgtcgata ccgtttgtat ttccagctac gatcacgaca accagcgcga gcttaaagtg
     9901 ctgaaacgcg cagaaggcga tggcgaaggc ttcatcgtta ttgatgacct ggtggatacc
     9961 ggtggtactg cggttgcgat tcgtgaaatg tatccaaaag cgcactttgt caccatcttc
    10021 gcaaaaccgg ctggtcgtcc gctggttgat gactatgttg ttgatatccc gcaagatacc
    10081 tggattgaac agccgtggga tatgggcgtc gtattcgtcc cgccaatctc cggtcgctaa
    10141 tcttttcaac gcctggcact gccgggcgtt gttcttttta acttcaggcg ggttacaata
    10201 gtttccagta agtattctgg aggctgcatc catgacacag gcaaacctga gcgaaaccct
    10261 gttcaaaccc cgctttaaac atcctgaaac ctcgacgcta gtccgccgct ttaatcacgg
    10321 cgcacaaccg cctgtgcagt cggcccttga tggtaaaacc atccctcact ggtatcgcat
    10381 gattaaccgt ctgatgtgga tctggcgcgg cattgaccca cgcgaaatcc tcgacgtcca
    10441 ggcacgtatt gtgatgagcg atgccgaacg taccgacgat gatttatacg atacggtgat
    10501 tggctaccgt ggcggcaact ggatttatga gtgggccccg gatctttgtg aaggaacctt
    10561 acttctgtgg tgtgacataa ttggacaaac tacctacaga gatttaaagc tctaaggtaa
    10621 atataaaatt tttaagtgta taatgtgtta aactactgat tctaattgtt tgtgtatttt
    10681 agattccaac ctatggaact gatgaatggg agcagtggtg gaatgccttt aatgaggaaa
    10741 acctgttttg ctcagaagaa atgccatcta gtgatgatga ggctactgct gactctcaac
    10801 attctactcc tccaaaaaag aagagaaagg tagaagaccc caaggacttt ccttcagaat
    10861 tgctaagttt tttgagtcat gctgtgttta gtaatagaac tcttgcttgc tttgctattt
    10921 acaccacaaa ggaaaaagct gcactgctat acaagaaaat tatggaaaaa tattctgtaa
    10981 cctttataag taggcataac agttataatc ataacatact gttttttctt actccacaca
    11041 ggcatagagt gtctgctatt aataactatg ctcaaaaatt gtgtaccttt agctttttaa
    11101 tttgtaaagg ggttaataag gaatatttga tgtatagtgc cttgactaga gatcataatc
    11161 agccatacca catttgtaga ggttttactt gctttaaaaa acctcccaca cctccccctg
    11221 aacctgaaac ataaaatgaa tgcaattgtt gttgttaact tgtttattgc agcttataat
    11281 ggttacaaat aaagcaatag catcacaaat ttcacaaata aagcattttt ttcactgcat
    11341 tctagttgtg gtttgtccaa actcatcaat gtatcttatc atgtctggat caactggata
    11401 actcaagcta accaaaatca tcccaaactt cccaccccat accctattac cactgccaat
    11461 tacctgtggt ttcatttact ctaaacctgt gattcctctg aattattttc attttaaaga
    11521 aattgtattt gttaaatatg tactacaaac ttagtagt
//
