LOCUS       sequence-212711-        9446 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses human codon optimized FnCpf1(RVR mutant) in mammalian 
            cells..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9446)
  AUTHORS   Toth E, Czene BC, Kulcsar PI, Krausz SL, Talas A, Nyeste A, Varga E,
            Huszar K, Weinhardt N, Ligeti Z, Borsy AE, Fodor E, Welker E
  TITLE     Mb- and FnCpf1 nucleases are active in mammalian cells: activities 
            and PAM preferences of four wild-type Cpf1 nucleases and of their 
            altered PAM specificity variants.
  JOURNAL   Nucleic Acids Res. 2018 Sep 20. pii: 5103950. doi: 
            10.1093/nar/gky815.
  PUBMED    30239882
REFERENCE   2  (bases 1 to 9446)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. 2018 Sep 20. pii: 5103950. doi: 10.1093/nar/gky815."
COMMENT     Sequence Label: pTE3314
FEATURES             Location/Qualifiers
     source          1..9446
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        127..506
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        507..710
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     661..681
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     755..774
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        755..773
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             4721..4768
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
     CDS             4775..4855
                     /codon_start=1
                     /product="three tandem HA epitope tags"
                     /label=3xHA
                     /translation="YPYDVPDYAYPYDVPDYAYPYDVPDYA"
     primer_bind     complement(4929..4946)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    4935..5159
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      5205..5633
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5292..5311)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5502..5523
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(5642..5662)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        5647..5976
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      5827..5962
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     5889..5908
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             6043..6837
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(6097..6116)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     6707..6726
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    7011..7132
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7048..7067)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7102..7121
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(7181..7197)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7181..7197)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7194..7216)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7205..7221
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7229..7235,7236..7253,7254..7259))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7274..7295
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7412..7429)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7583..8171)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7663..7682)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8342..9133,9134..9202))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8965..8984
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9203..9307)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(9382..9401)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 ctgagtagtg cgcgagcaaa atttaagcta caacaaggca aggcttgacc gacaattgca
       61 tgaagaatct gcttagggtt aggcgttttg cgctgcttcg cgatgtacgg gccagatata
      121 cgcgttgaca ttgattattg actagttatt aatagtaatc aattacgggg tcattagttc
      181 atagcccata tatggagttc cgcgttacat aacttacggt aaatggcccg cctggctgac
      241 cgcccaacga cccccgccca ttgacgtcaa taatgacgta tgttcccata gtaacgccaa
      301 tagggacttt ccattgacgt caatgggtgg agtatttacg gtaaactgcc cacttggcag
      361 tacatcaagt gtatcatatg ccaagtacgc cccctattga cgtcaatgac ggtaaatggc
      421 ccgcctggca ttatgcccag tacatgacct tatgggactt tcctacttgg cagtacatct
      481 acgtattagt catcgctatt accatggtga tgcggttttg gcagtacatc aatgggcgtg
      541 gatagcggtt tgactcacgg ggatttccaa gtctccaccc cattgacgtc aatgggagtt
      601 tgttttggca ccaaaatcaa cgggactttc caaaatgtcg taacaactcc gccccattga
      661 cgcaaatggg cggtaggcgt gtacggtggg aggtctatat aagcagagct ctctggctaa
      721 ctagagaacc cactgcttac tggcttatcg aaattaatac gactcactat agggagaccc
      781 aagctggcta gcgtttaaac ttaagcttgg taccgccacc atgagcatct accaggagtt
      841 cgtcaacaag tattcactga gtaagacact gcggttcgag ctgatcccac agggcaagac
      901 actggagaac atcaaggccc gaggcctgat tctggacgat gagaagcggg caaaagacta
      961 taagaaagcc aagcagatca ttgataaata ccaccagttc tttatcgagg aaattctgag
     1021 ctccgtgtgc atcagtgagg atctgctgca gaattactca gacgtgtact tcaagctgaa
     1081 gaagagcgac gatgacaacc tgcagaagga cttcaagtcc gccaaggaca ccatcaagaa
     1141 acagattagc gagtacatca aggactccga aaagtttaaa aatctgttca accagaatct
     1201 gatcgatgct aagaaaggcc aggagtccga cctgatcctg tggctgaaac agtctaagga
     1261 caatgggatt gaactgttca aggctaactc cgatatcact gatattgacg aggcactgga
     1321 aatcatcaag agcttcaagg gatggaccac atactttaaa ggcttccacg agaaccgcaa
     1381 gaacgtgtac tccagcaacg acattcctac ctccatcatc taccgaatcg tcgatgacaa
     1441 tctgccaaag ttcctggaga acaaggccaa atatgaatct ctgaaggaca aagctcccga
     1501 ggcaattaat tacgaacaga tcaagaaaga tctggctgag gaactgacat tcgatatcga
     1561 ctataagact agcgaggtga accagagggt cttttccctg gacgaggtgt ttgaaatcgc
     1621 caatttcaac aattacctga accagtccgg cattactaaa ttcaatacca tcattggcgg
     1681 gaagtttgtg aacggggaga ataccaagcg caagggaatt aacgaataca tcaatctgta
     1741 tagccagcag atcaacgaca aaactctgaa gaaatacaag atgtctgtgc tgttcaaaca
     1801 gatcctgagt gataccgagt ccaagtcttt tgtcattgat aaactggaag atgactcaga
     1861 cgtggtcact accatgcaga gcttttatga gcagatcgcc gctttcaaga cagtggagga
     1921 aaaatctatt aaggaaactc tgagtctgct gttcgatgac ctgaaagccc agaagctgga
     1981 cctgagtaag atctacttca aaaacgataa gagtctgaca gacctgtcac agcaggtgtt
     2041 tgatgactat tccgtgattg ggaccgccgt cctggagtac attacacagc agatcgctcc
     2101 aaagaacctg gataatccct ctaagaaaga gcaggaactg atcgctaaga aaaccgagaa
     2161 ggcaaaatat ctgagtctgg aaacaattaa gctggcactg gaggagttca acaagcacag
     2221 ggatattgac aaacagtgcc gctttgagga aatcctggcc aacttcgcag ccatccccat
     2281 gatttttgat gagatcgccc agaacaaaga caatctggct cagatcagta ttaagtacca
     2341 gaaccagggc aagaaagacc tgctgcaggc ttcagcagaa gatgacgtga aagccatcaa
     2401 ggatctgctg gaccagacca acaatctgct gcacaagctg aaaatcttcc atattagtca
     2461 gtcagaggat aaggctaata tcctggataa agacgaacac ttctacctgg tgttcgagga
     2521 atgttacttc gagctggcaa acattgtccc cctgtataac aagattagga actacatcac
     2581 acagaagcct tactctgacg agaagtttaa actgaacttc gaaaatagta cgctagccag
     2641 ggggtgggat aagaacgtgg agcctgacag gacagctatc ctgttcatca aggatgacaa
     2701 gtactatctg ggagtgatga ataagaaaaa caataagatc ttcgatgaca aagccattaa
     2761 ggagaacaaa ggggaaggat acaagaaaat cgtgtataag ctgctgcccg gcgcaaataa
     2821 gatgctgcct aaggtgttct tcagcgccaa gagtatcaaa ttctacaacc catccgagga
     2881 catcctgcgg attagaaatc actcaacaca tactaagaac gggagccccc agaagggata
     2941 tgagaaattt gagttcaaca tcgaggattg caggaagttt attgacttct acaagcagag
     3001 catctccaaa caccctgaat ggaaggattt tggcttccgg ttttccgaca cacagagata
     3061 taactctatc gacgagttct accgcgaggt ggaaaatcag gggtataagc tgacttttga
     3121 gaacatttct gaaagttaca tcgacagcgt ggtcaatcag ggaaagctgt acctgttcca
     3181 gatctataac aaagattttt cagcatacag caagggcaga ccaaacctgc atacactgta
     3241 ctggaaggcc ctgttcgatg agaggaatct gcaggacgtg gtctataaac tgaacggaga
     3301 ggccgaactg ttttaccgga agcagtctat tcctaagaaa atcactcacc cagctaagga
     3361 ggccatcgct aacaagaaca aggacaatcc taagaaagag agcgtgttcg aatacgatct
     3421 gattaaggac aagcggttca ccgaagataa gttctttttc cattgtccaa tcaccattaa
     3481 cttcaagtca agcggcgcta acaagttcaa cgacgagatc aatctgctgc tgaaggaaaa
     3541 agcaaacgat gtgcacatcc tgagcattga ccgaggagag cggcatctgg cctactatac
     3601 cctggtggat ggcaaaggga atatcattaa gcaggataca ttcaacatca ttggcaatga
     3661 ccggatgaaa accaactacc acgataaact ggctgcaatc gagaaggata gagactcagc
     3721 taggaaggac tggaagaaaa tcaacaacat taaggagatg aaggaaggct atctgagcca
     3781 ggtggtccat gagattgcaa agctggtcat cgaatacaat gccattgtgg tgttcgagga
     3841 tctgaacttc ggctttaaga gggggcgctt taaggtggaa aaacaggtct atcagaagct
     3901 ggagaaaatg ctgatcgaaa agctgaatta cctggtgttt aaagataacg agttcgacaa
     3961 gaccggaggc gtcctgagag cctaccagct gacagctccc tttgaaactt tcaagaaaat
     4021 gggaaaacag acaggcatca tctactatgt gccagccgga ttcacttcca agatctgccc
     4081 cgtgaccggc tttgtcaacc agctgtaccc taaatatgag tcagtgagca agtcccagga
     4141 atttttcagc aagttcgata agatctgtta taatctggac aaggggtact tcgagttttc
     4201 cttcgattac aagaacttcg gcgacaaggc cgctaagggg aaatggacca ttgcctcctt
     4261 cggatctcgc ctgatcaact ttcgaaattc cgataaaaac cacaattggg acactaggga
     4321 ggtgtaccca accaaggagc tggaaaagct gctgaaagac tactctatcg agtatggaca
     4381 tggcgaatgc atcaaggcag ccatctgtgg cgagagtgat aagaaatttt tcgccaagct
     4441 gacctcagtg ctgaatacaa tcctgcagat gcggaactca aagaccggga cagaactgga
     4501 ctatctgatt agccccgtgg ctgatgtcaa cggaaacttc ttcgacagca gacaggcacc
     4561 caaaaatatg cctcaggatg cagacgccaa cggggcctac cacatcgggc tgaagggact
     4621 gatgctgctg ggccggatca agaacaatca ggaggggaag aagctgaacc tggtcattaa
     4681 gaacgaggaa tacttcgagt ttgtccagaa tagaaataac aaaaggccgg cggccacgaa
     4741 aaaggccggc caggcaaaaa agaaaaaggg atcctaccca tacgatgttc cagattacgc
     4801 ttatccctac gacgtgcctg attatgcata cccatatgat gtccccgact atgcctaaga
     4861 attctgcaga tatccagcac agtggcggcc gctcgagtct agagggcccg tttaaacccg
     4921 ctgatcagcc tcgactgtgc cttctagttg ccagccatct gttgtttgcc cctcccccgt
     4981 gccttccttg accctggaag gtgccactcc cactgtcctt tcctaataaa atgaggaaat
     5041 tgcatcgcat tgtctgagta ggtgtcattc tattctgggg ggtggggtgg ggcaggacag
     5101 caagggggag gattgggaag acaatagcag gcatgctggg gatgcggtgg gctctatggc
     5161 ttctgaggcg gaaagaacca gctggggctc tagggggtat ccccacgcgc cctgtagcgg
     5221 cgcattaagc gcggcgggtg tggtggttac gcgcagcgtg accgctacac ttgccagcgc
     5281 cctagcgccc gctcctttcg ctttcttccc ttcctttctc gccacgttcg ccggctttcc
     5341 ccgtcaagct ctaaatcggg ggctcccttt agggttccga tttagtgctt tacggcacct
     5401 cgaccccaaa aaacttgatt agggtgatgg ttcacgtagt gggccatcgc cctgatagac
     5461 ggtttttcgc cctttgacgt tggagtccac gttctttaat agtggactct tgttccaaac
     5521 tggaacaaca ctcaacccta tctcggtcta ttcttttgat ttataaggga ttttgccgat
     5581 ttcggcctat tggttaaaaa atgagctgat ttaacaaaaa tttaacgcga attaattctg
     5641 tggaatgtgt gtcagttagg gtgtggaaag tccccaggct ccccagcagg cagaagtatg
     5701 caaagcatgc atctcaatta gtcagcaacc aggtgtggaa agtccccagg ctccccagca
     5761 ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa ccatagtccc gcccctaact
     5821 ccgcccatcc cgcccctaac tccgcccagt tccgcccatt ctccgcccca tggctgacta
     5881 atttttttta tttatgcaga ggccgaggcc gcctctgcct ctgagctatt ccagaagtag
     5941 tgaggaggct tttttggagg cctaggcttt tgcaaaaagc tcccgggagc ttgtatatcc
     6001 attttcggat ctgatcaaga gacaggatga ggatcgtttc gcatgattga acaagatgga
     6061 ttgcacgcag gttctccggc cgcttgggtg gagaggctat tcggctatga ctgggcacaa
     6121 cagacaatcg gctgctctga tgccgccgtg ttccggctgt cagcgcaggg gcgcccggtt
     6181 ctttttgtca agaccgacct gtccggtgcc ctgaatgaac tgcaggacga ggcagcgcgg
     6241 ctatcgtggc tggccacgac gggcgttcct tgcgcagctg tgctcgacgt tgtcactgaa
     6301 gcgggaaggg actggctgct attgggcgaa gtgccggggc aggatctcct gtcatctcac
     6361 cttgctcctg ccgagaaagt atccatcatg gctgatgcaa tgcggcggct gcatacgctt
     6421 gatccggcta cctgcccatt cgaccaccaa gcgaaacatc gcatcgagcg agcacgtact
     6481 cggatggaag ccggtcttgt cgatcaggat gatctggacg aagagcatca ggggctcgcg
     6541 ccagccgaac tgttcgccag gctcaaggcg cgcatgcccg acggcgagga tctcgtcgtg
     6601 acccatggcg atgcctgctt gccgaatatc atggtggaaa atggccgctt ttctggattc
     6661 atcgactgtg gccggctggg tgtggcggac cgctatcagg acatagcgtt ggctacccgt
     6721 gatattgctg aagagcttgg cggcgaatgg gctgaccgct tcctcgtgct ttacggtatc
     6781 gccgctcccg attcgcagcg catcgccttc tatcgccttc ttgacgagtt cttctgagcg
     6841 ggactctggg gttcgaaatg accgaccaag cgacgcccaa cctgccatca cgagatttcg
     6901 attccaccgc cgccttctat gaaaggttgg gcttcggaat cgttttccgg gacgccggct
     6961 ggatgatcct ccagcgcggg gatctcatgc tggagttctt cgcccacccc aacttgttta
     7021 ttgcagctta taatggttac aaataaagca atagcatcac aaatttcaca aataaagcat
     7081 ttttttcact gcattctagt tgtggtttgt ccaaactcat caatgtatct tatcatgtct
     7141 gtataccgtc gacctctagc tagagcttgg cgtaatcatg gtcatagctg tttcctgtgt
     7201 gaaattgtta tccgctcaca attccacaca acatacgagc cggaagcata aagtgtaaag
     7261 cctggggtgc ctaatgagtg agctaactca cattaattgc gttgcgctca ctgcccgctt
     7321 tccagtcggg aaacctgtcg tgccagctgc attaatgaat cggccaacgc gcggggagag
     7381 gcggtttgcg tattgggcgc tcttccgctt cctcgctcac tgactcgctg cgctcggtcg
     7441 ttcggctgcg gcgagcggta tcagctcact caaaggcggt aatacggtta tccacagaat
     7501 caggggataa cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta
     7561 aaaaggccgc gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa
     7621 atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc
     7681 cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt
     7741 ccgcctttct cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca
     7801 gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg
     7861 accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat
     7921 cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta
     7981 cagagttctt gaagtggtgg cctaactacg gctacactag aagaacagta tttggtatct
     8041 gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac
     8101 aaaccaccgc tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa
     8161 aaggatctca agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa
     8221 actcacgtta agggattttg gtcatgagat tatcaaaaag gatcttcacc tagatccttt
     8281 taaattaaaa atgaagtttt aaatcaatct aaagtatata tgagtaaact tggtctgaca
     8341 gttaccaatg cttaatcagt gaggcaccta tctcagcgat ctgtctattt cgttcatcca
     8401 tagttgcctg actccccgtc gtgtagataa ctacgatacg ggagggctta ccatctggcc
     8461 ccagtgctgc aatgataccg cgagacccac gctcaccggc tccagattta tcagcaataa
     8521 accagccagc cggaagggcc gagcgcagaa gtggtcctgc aactttatcc gcctccatcc
     8581 agtctattaa ttgttgccgg gaagctagag taagtagttc gccagttaat agtttgcgca
     8641 acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat
     8701 tcagctccgg ttcccaacga tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag
     8761 cggttagctc cttcggtcct ccgatcgttg tcagaagtaa gttggccgca gtgttatcac
     8821 tcatggttat ggcagcactg cataattctc ttactgtcat gccatccgta agatgctttt
     8881 ctgtgactgg tgagtactca accaagtcat tctgagaata gtgtatgcgg cgaccgagtt
     8941 gctcttgccc ggcgtcaata cgggataata ccgcgccaca tagcagaact ttaaaagtgc
     9001 tcatcattgg aaaacgttct tcggggcgaa aactctcaag gatcttaccg ctgttgagat
     9061 ccagttcgat gtaacccact cgtgcaccca actgatcttc agcatctttt actttcacca
     9121 gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga ataagggcga
     9181 cacggaaatg ttgaatactc atactcttcc tttttcaata ttattgaagc atttatcagg
     9241 gttattgtct catgagcgga tacatatttg aatgtattta gaaaaataaa caaatagggg
     9301 ttccgcgcac atttccccga aaagtgccac ctgacgtcga cggatcggga gatctcccga
     9361 tcccctatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatct
     9421 gctccctgct tgtgtgttgg aggtcg
//
