LOCUS       sequence-212713-        8745 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian expression plasmid for BG505 SOSIP.664 fused to a 
            transmembrane tether.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 8745)
  AUTHORS   Heredia JD, Park J, Choi H, Gill KS, Procko E
  TITLE     Conformational engineering of HIV-1 Env based on mutational 
            tolerance in the CD4 and PG16 bound states.
  JOURNAL   J Virol. 2019 Mar 20. pii: JVI.00219-19. doi: 10.1128/JVI.00219-19.
  PUBMED    30894475
REFERENCE   2  (bases 1 to 8745)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Virol. 
            2019 Mar 20. pii: JVI.00219-19. doi: 10.1128/JVI.00219-19."
COMMENT     Sequence Label: pCEP4-BG505.SOSIP-TM
FEATURES             Location/Qualifiers
     source          1..8745
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        86..231
                     /label=HSV TK promoter
                     /note="herpes simplex virus thymidine kinase promoter"
     CDS             274..1311
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPDRE
                     MGEAN"
     primer_bind     complement(1309..1328)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    1353..1401
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     enhancer        2055..2434
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2435..2638
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2589..2609
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     2635..2659
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             4691..4708
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     polyA_signal    4854..4988
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4878..4897)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     4932..4951
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(5131..5153)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     rep_origin      5713..7502
                     /label=oriP
                     /note="Epstein-Barr virus oriP replication origin (Yates et
                     al., 2000)"
ORIGIN
        1 gccctgcttc atccccgtgg cccgttgctc gcgtttgctg gcggtgtccc cggaagaaat
       61 atatttgcat gtctttagtt ctatgatgac acaaaccccg cccagcgtct tgtcattggc
      121 gaattcgaac acgcagatgc agtcggggcg gcgcggtccg aggtccactt cgcatattaa
      181 ggtgacgcgt gtggcctcga acaccgagcg accctgcagc gacccgctta acagcgtcaa
      241 cagcgtgccg cagatcccgg ggggcaatga gatatgaaaa agcctgaact caccgcgacg
      301 tctgtcgaga agtttctgat cgaaaagttc gacagcgtct ccgacctgat gcagctctcg
      361 gagggcgaag aatctcgtgc tttcagcttc gatgtaggag ggcgtggata tgtcctgcgg
      421 gtaaatagct gcgccgatgg tttctacaaa gatcgttatg tttatcggca ctttgcatcg
      481 gccgcgctcc cgattccgga agtgcttgac attggggaat tcagcgagag cctgacctat
      541 tgcatctccc gccgtgcaca gggtgtcacg ttgcaagacc tgcctgaaac cgaactgccc
      601 gctgttctgc agccggtcgc ggaggccatg gatgcgatcg ctgcggccga tcttagccag
      661 acgagcgggt tcggcccatt cggaccgcaa ggaatcggtc aatacactac atggcgtgat
      721 ttcatatgcg cgattgctga tccccatgtg tatcactggc aaactgtgat ggacgacacc
      781 gtcagtgcgt ccgtcgcgca ggctctcgat gagctgatgc tttgggccga ggactgcccc
      841 gaagtccggc acctcgtgca cgcggatttc ggctccaaca atgtcctgac ggacaatggc
      901 cgcataacag cggtcattga ctggagcgag gcgatgttcg gggattccca atacgaggtc
      961 gccaacatct tcttctggag gccgtggttg gcttgtatgg agcagcagac gcgctacttc
     1021 gagcggaggc atccggagct tgcaggatcg ccgcggctcc gggcgtatat gctccgcatt
     1081 ggtcttgacc aactctatca gagcttggtt gacggcaatt tcgatgatgc agcttgggcg
     1141 cagggtcgat gcgacgcaat cgtccgatcc ggagccggga ctgtcgggcg tacacaaatc
     1201 gcccgcagaa gcgcggccgt ctggaccgat ggctgtgtag aagtactcgc cgatagtgga
     1261 aaccgacgcc ccagcactcg tccggatcgg gagatggggg aggctaactg aaacacggaa
     1321 ggagacaata ccggaaggaa cccgcgctat gacggcaata aaaagacaga ataaaacgca
     1381 cgggtgttgg gtcgtttgtt cataaacgcg gggttcggtc ccagggctgg cactctgtcg
     1441 ataccccacc gagaccccat tggggccaat acgcccgcgt ttcttccttt tccccacccc
     1501 accccccaag ttcgggtgaa ggcccagggc tcgcagccaa cgtcggggcg gcaggccctg
     1561 ccatagccac tggccccgtg ggttagggac ggggtccccc atggggaatg gtttatggtt
     1621 cgtgggggtt attattttgg gcgttgcgtg gggtcaggtc cacgactgga ctgagcagac
     1681 agacccatgg tttttggatg gcctgggcat ggaccgcatg tactggcgcg acacgaacac
     1741 cgggcgtctg tggctgccaa acacccccga cccccaaaaa ccaccgcgcg gatttctggc
     1801 gtgccaagct agtcgaccaa ttctcatgtt tgacagctta tcatcgcaga tccgggcaac
     1861 gttgttgcca ttgctgcagg cgcagaactg gtaggtatgg aagatctata cattgaatca
     1921 atattggcaa ttagccatat tagtcattgg ttatatagca taaatcaata ttggctattg
     1981 gccattgcat acgttgtatc tatatcataa tatgtacatt tatattggct catgtccaat
     2041 atgaccgcca tgttgacatt gattattgac tagttattaa tagtaatcaa ttacggggtc
     2101 attagttcat agcccatata tggagttccg cgttacataa cttacggtaa atggcccgcc
     2161 tggctgaccg cccaacgacc cccgcccatt gacgtcaata atgacgtatg ttcccatagt
     2221 aacgccaata gggactttcc attgacgtca atgggtggag tatttacggt aaactgccca
     2281 cttggcagta catcaagtgt atcatatgcc aagtccgccc cctattgacg tcaatgacgg
     2341 taaatggccc gcctggcatt atgcccagta catgacctta cgggactttc ctacttggca
     2401 gtacatctac gtattagtca tcgctattac catggtgatg cggttttggc agtacaccaa
     2461 tgggcgtgga tagcggtttg actcacgggg atttccaagt ctccacccca ttgacgtcaa
     2521 tgggagtttg ttttggcacc aaaatcaacg ggactttcca aaatgtcgta ataaccccgc
     2581 cccgttgacg caaatgggcg gtaggcgtgt acggtgggag gtctatataa gcagagctcg
     2641 tttagtgaac cgtcagatct ctagaagctg ggtaccagct gctagcgcca ccatgccaat
     2701 gggctccctt cagcccttgg ctaccctgta ccttcttggg atgttggtcg catctgtgct
     2761 tgccgcagaa aacttgtggg taactgtcta ctatggagta cccgtttgga aagacgcaga
     2821 aacaacgctt ttttgcgcat ccgacgcgaa ggcatacgaa acggaaaagc acaatgtttg
     2881 ggcaacgcat gcctgcgtcc cgacagaccc aaatcctcag gagattcacc ttgagaatgt
     2941 aacagaggaa ttcaacatgt ggaagaataa catggtagaa caaatgcata cggatattat
     3001 cagtctttgg gaccagagcc ttaaaccttg cgttaaactt acgcccctct gcgtaactct
     3061 gcaatgtact aacgtaacga ataacattac ggacgacatg cgcggcgaac tgaagaattg
     3121 cagctttaat atgacaacgg agttgcgaga taaaaagcag aaggtgtaca gcctcttcta
     3181 caggcttgac gttgtgcaaa taaacgaaaa ccaggggaac aggagcaata actccaataa
     3241 agaatacaga ttgatcaact gtaacactag cgcaataacc caggcctgcc cgaaggtgtc
     3301 atttgagccg attcctatac actattgcgc cccggcaggg tttgcgatct tgaaatgcaa
     3361 ggataagaag tttaatggaa ctggtccctg tcccagtgtg agtacggttc agtgtaccca
     3421 cggcataaag ccagtggtat ccactcagct tctcctcaat ggcagcctcg cagaggaaga
     3481 ggtcatgata cggagtgaga atattactaa caatgccaag aatatcttgg tccaatttaa
     3541 tacccccgtc cagattaatt gtacccgacc taataataat accagaaaat ccattcgcat
     3601 cggaccaggt caggcgttct atgccacggg agatataata ggtgacatcc gacaggcgca
     3661 ttgcaatgta agcaaggcca catggaatga gactctgggt aaagttgtca agcagctcag
     3721 gaagcatttc gggaacaata ccatcatccg atttgcgaac agtagtgggg gtgatcttga
     3781 agtaactaca cattcattta actgcggcgg ggagtttttt tattgtaaca catccgggtt
     3841 gttcaactcc acttggatat ctaacactag tgttcaggga tcaaactcca caggatcaaa
     3901 cgatagtata acacttcctt gtcgcatcaa acagattatc aacatgtggc agcgaatcgg
     3961 ccaggcgatg tacgccccgc ctatccaggg agtaatacgg tgcgtaagca acataacggg
     4021 ccttatactt acgcgcgatg gaggcagcac taattctacc actgagacgt tccggcctgg
     4081 cggtggggac atgcgagaca actggcggtc tgagttgtac aagtacaaag tggttaaaat
     4141 cgaacccctg ggtgtcgccc caacgagatg taaacgacgg gtagtgggaa ggaggagaag
     4201 gcgacgggcg gtcggcattg gggccgtttt cttgggtttt ctgggggcgg ctgggagcac
     4261 tatgggagca gctagtatga ccctgaccgt acaggcgagg aacctcctgt ccggtatagt
     4321 gcaacaacag tcaaaccttc ttagggctcc tgaggcgcag caacatctcc ttaagttgac
     4381 tgtctggggc ataaagcaac tccaagcgcg ggttctcgcg gtggaaagat accttaggga
     4441 ccaacaactt ttggggattt ggggttgttc aggcaaactc atctgttgca ctaacgttcc
     4501 ttggaactcc tcatggtcta accgaaatct ctccgagatc tgggataata tgacatggtt
     4561 gcagtgggat aaggagatta gcaattacac tcaaataatc tatgggctcc tggaggaaag
     4621 ccagaatcag caggagaaga atgaacagga tcttctggcc cttgatgggt caggctctgg
     4681 aggatctgga caccatcatc atcaccatgg gtccggcgct ggttctgcta tccccatcat
     4741 gggtatcgtt gctggcctgg ttgtccttgc agctgtagtc actggagctg cggtcgctgc
     4801 tgtgctgtgg agaaagaaga gctcagatta gtaactcgag gccggcaagg ccggatccag
     4861 acatgataag atacattgat gagtttggac aaaccacaac tagaatgcag tgaaaaaaat
     4921 gctttatttg tgaaatttgt gatgctattg ctttatttgt aaccattata agctgcaata
     4981 aacaagttaa caacaacaat tgcattcatt ttatgtttca ggttcagggg gaggtgtggg
     5041 aggtttttta aagcaagtaa aacctctaca aatgtggtat ggctgattat gatccggctg
     5101 cctcgcgcgt ttcggtgatg acggtgaaaa cctctgacac atgcagctcc cggagacggt
     5161 cacagcttgt ctgtaagcgg atgccgggag cagacaagcc cgtcagggcg cgtcagcggg
     5221 tgttggcggg tgtcggggcg cagccatgag gtcgactcta gaggatcgat ccccgccccg
     5281 gacgaactaa acctgactac gacatctctg ccccttcttc gcggggcagt gcatgtaatc
     5341 ccttcagttg gttggtacaa cttgccaact gggccctgtt ccacatgtga cacggggggg
     5401 gaccaaacac aaaggggttc tctgactgta gttgacatcc ttataaatgg atgtgcacat
     5461 ttgccaacac tgagtggctt tcatcctgga gcagactttg cagtctgtgg actgcaacac
     5521 aacattgcct ttatgtgtaa ctcttggctg aagctcttac accaatgctg ggggacatgt
     5581 acctcccagg ggcccaggaa gactacggga ggctacacca acgtcaatca gaggggcctg
     5641 tgtagctacc gataagcgga ccctcaagag ggcattagca atagtgttta taaggccccc
     5701 ttgttaaccc taaacgggta gcatatgctt cccgggtagt agtatatact atccagacta
     5761 accctaattc aatagcatat gttacccaac gggaagcata tgctatcgaa ttagggttag
     5821 taaaagggtc ctaaggaaca gcgatatctc ccaccccatg agctgtcacg gttttattta
     5881 catggggtca ggattccacg agggtagtga accattttag tcacaagggc agtggctgaa
     5941 gatcaaggag cgggcagtga actctcctga atcttcgcct gcttcttcat tctccttcgt
     6001 ttagctaata gaataactgc tgagttgtga acagtaaggt gtatgtgagg tgctcgaaaa
     6061 caaggtttca ggtgacgccc ccagaataaa atttggacgg ggggttcagt ggtggcattg
     6121 tgctatgaca ccaatataac cctcacaaac cccttgggca ataaatacta gtgtaggaat
     6181 gaaacattct gaatatcttt aacaatagaa atccatgggg tggggacaag ccgtaaagac
     6241 tggatgtcca tctcacacga atttatggct atgggcaaca cataatccta gtgcaatatg
     6301 atactggggt tattaagatg tgtcccaggc agggaccaag acaggtgaac catgttgtta
     6361 cactctattt gtaacaaggg gaaagagagt ggacgccgac agcagcggac tccactggtt
     6421 gtctctaaca cccccgaaaa ttaaacgggg ctccacgcca atggggccca taaacaaaga
     6481 caagtggcca ctcttttttt tgaaattgtg gagtgggggc acgcgtcagc ccccacacgc
     6541 cgccctgcgg ttttggactg taaaataagg gtgtaataac ttggctgatt gtaaccccgc
     6601 taaccactgc ggtcaaacca cttgcccaca aaaccactaa tggcaccccg gggaatacct
     6661 gcataagtag gtgggcgggc caagataggg gcgcgattgc tgcgatctgg aggacaaatt
     6721 acacacactt gcgcctgagc gccaagcaca gggttgttgg tcctcatatt cacgaggtcg
     6781 ctgagagcac ggtgggctaa tgttgccatg ggtagcatat actacccaaa tatctggata
     6841 gcatatgcta tcctaatcta tatctgggta gcataggcta tcctaatcta tatctgggta
     6901 gcatatgcta tcctaatcta tatctgggta gtatatgcta tcctaattta tatctgggta
     6961 gcataggcta tcctaatcta tatctgggta gcatatgcta tcctaatcta tatctgggta
     7021 gtatatgcta tcctaatctg tatccgggta gcatatgcta tcctaataga gattagggta
     7081 gtatatgcta tcctaattta tatctgggta gcatatacta cccaaatatc tggatagcat
     7141 atgctatcct aatctatatc tgggtagcat atgctatcct aatctatatc tgggtagcat
     7201 aggctatcct aatctatatc tgggtagcat atgctatcct aatctatatc tgggtagtat
     7261 atgctatcct aatttatatc tgggtagcat aggctatcct aatctatatc tgggtagcat
     7321 atgctatcct aatctatatc tgggtagtat atgctatcct aatctgtatc cgggtagcat
     7381 atgctatcct catgcatata cagtcagcat atgataccca gtagtagagt gggagtgcta
     7441 tcctttgcat atgccgccac ctcccaaggg ggcgtgaatt ttcgctgctt gtccttttcc
     7501 tgctggttgc tcccattctt aggtgaattt aaggaggcca ggctaaagcc gtcgcatgtc
     7561 tgattgctca ccaggtaaat gtcgctaatg ttttccaacg cgagaaggtg ttgagcgcgg
     7621 agctgagtga cgtgacaaca tgggtatgcc caattgcccc atgttgggag gacgaaaatg
     7681 gtgacaagac agatggccag aaatacacca acagcacgca tgatgtctac tggggattta
     7741 ttctttagtg cgggggaata cacggctttt aatacgattg agggcgtctc ctaacaagtt
     7801 acatcactcc tgcccttcct caccctcatc tccatcacct ccttcatctc cgtcatctcc
     7861 gtcatcaccc tccgcggcag ccccttccac cataggtgga aaccagggag gcaaatctac
     7921 tccatcgtca aagctgcaca cagtcaccct gatattgcag gtaggagcgg gctttgtcat
     7981 aacaaggtcc ttaatcgcat ccttcaaaac ctcagcaaat atatgagttt gtaaaaagac
     8041 catgaaataa cagacaatgg actcccttag cgggccaggt tgtgggccgg gtccaggggc
     8101 cattccaaag gggagacgac tcaatggtgt aagacgacat tgtggaatag caagggcagt
     8161 tcctcgcctt aggttgtaaa gggaggtctt actacctcca tatacgaaca caccggcgac
     8221 ccaagttcct tcgtcggtag tcctttctac gtgactccta gccaggagag ctcttaaacc
     8281 ttctgcaatg ttctcaaatt tcgggttgga acctccttga ccacgatgct ttccaaacca
     8341 ccctcctttt ttgcgcctgc ctccatcacc ctgaccccgg ggtccagtgc ttgggccttc
     8401 tcctgggtca tctgcggggc cctgctctat cgctcccggg ggcacgtcag gctcaccatc
     8461 tgggccacct tcttggtggt attcaaaata atcggcttcc cctacagggt ggaaaaatgg
     8521 ccttctacct ggagggggcc tgcgcggtgg agacccggat gatgatgact gactactggg
     8581 actcctgggc ctcttttctc cacgtccacg acctctcccc ctggctcttt cacgacttcc
     8641 ccccctggct ctttcacgtc ctctaccccg gcggcctcca ctacctcctc gaccccggcc
     8701 tccactacct cctcgacccc ggcctccact gcctcctcga ccccg
//
