LOCUS       sequence-212731-         953 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Express ELANE-cleaved mouse GSDMD in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 953)
  AUTHORS   Kambara H, Liu F, Zhang X, Liu P, Bajrami B, Teng Y, Zhao L, Zhou S,
            Yu H, Zhou W, Silberstein LE, Cheng T, Han M, Xu Y, Luo HR
  TITLE     Gasdermin D Exerts Anti-inflammatory Effects by Promoting Neutrophil
            Death.
  JOURNAL   Cell Rep. 2018 Mar 13;22(11):2924-2936. doi: 
            10.1016/j.celrep.2018.02.067.
  PUBMED    29539421
REFERENCE   2  (bases 1 to 953)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.celrep.2018.02"; journalName: "Cell Rep"; date: 
            "2018-03-13- 13"; volume: "22"; issue: "11"; pages: "2924-2936"
COMMENT     Sequence Label: pCAG-mGSDMD1-251
FEATURES             Location/Qualifiers
     source          1..953
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             838..861
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             868..891
                     /codon_start=1
                     /product="peptide that binds Strep-Tactin(R), an engineered
                     form of streptavidin"
                     /label=Strep-Tag II
                     /translation="WSHPQFEK"
     CDS             928..951
                     /codon_start=1
                     /product="peptide that binds Strep-Tactin(R), an engineered
                     form of streptavidin"
                     /label=Strep-Tag II
                     /translation="WSHPQFEK"
ORIGIN
        1 ccggaggtac ccgggcggcc gcgagtcttc gaattcaaac gcgtctcgag caccatgcca
       61 tcggcctttg agaaagtggt caagaatgtg atcaaggagg taagcggcag cagaggcgat
      121 ctcattccgg tggacagcct gcggaactcc accagcttca ggccctactg ccttctgaac
      181 aggaaatttt caagctcaag gttctggaaa ccccgttatt catgtgtcaa cctgtcaatc
      241 aaggacatcc tggagcccag tgctccagaa ccagaaccgg agtgttttgg ctccttcaaa
      301 gtctctgatg tcgtcgatgg gaacattcag ggcagagtga tgttgtcagg catgggagaa
      361 gggaaaattt ctggtggggc tgcagtgtct gacagttcca gtgcctccat gaatgtgtgt
      421 atactgcgtg tgactcagaa gacctgggag accatgcagc atgaaaggca ccttcagcag
      481 cctgagaaca aaatcctgca acagcttcgg agtcgtgggg atgacctgtt tgtggtgacc
      541 gaggtgctgc agacaaagga ggaagtgcag atcactgagg tccacagcca agagggctca
      601 ggccagttta cactgcctgg agctttatgc ttgaagggtg aaggcaaggg ccaccaaagc
      661 cggaagaaga tggtgaccat tcctgcaggc agcatcctgg cattccgagt ggcccaactg
      721 cttattggct ctaaatggga tatccttctc gtctcagatg agaaacagag gacctttgag
      781 ccctcctcag gtgacagaaa agcagtgctc gagaagcttg ctagcagatc tggattcgat
      841 tacaaggatg acgatgacaa gagcgcttgg agccacccgc agttcgagaa aggtggaggt
      901 tccggaggtg gatcgggagg tggatcgtgg agccacccgc agttcgaaaa ata
//
