LOCUS       sequence-212734-        5317 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  AAV inverted terminal repeat based vector plasmid encoding the 
            C-terminal half of nCas9.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5317)
  AUTHORS   Ryu SM, Koo T, Kim K, Lim K, Baek G, Kim ST, Kim HS, Kim DE, Lee H, 
            Chung E, Kim JS
  TITLE     Adenine base editing in mouse embryos and an adult mouse model of 
            Duchenne muscular dystrophy.
  JOURNAL   Nat Biotechnol. 2018 Jul;36(6):536-539. doi: 10.1038/nbt.4148. Epub 
            2018 Apr 27.
  PUBMED    29702637
REFERENCE   2  (bases 1 to 5317)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/nbt.4148"; 
            journalName: "Nat Biotechnol"; date: "2018-07"; volume: "36"; issue:
            "6"; pages: "536-539"
COMMENT     Sequence Label: pAAV-ABE-CT
FEATURES             Location/Qualifiers
     source          1..5317
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     repeat_region   1..130
                     /label=AAV2 ITR (alternate)
                     /note="Functional equivalent of wild-type AAV2 ITR"
     CDS             2202..2222
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             2223..2249
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /note="This is a variant of the standard HA tag, which has 
                     sequence YPYDVPDYA. Some anti-HA antibodies may not 
                     recognize this variant."
                     /translation="YPYDVPDYA"
     CDS             2223..2249
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     promoter        complement(2283..2301)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(2284..2301)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(2321..2338)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    2327..2551
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     repeat_region   2580..2720
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     repeat_region   2580..2709
                     /label=AAV2 ITR
     rep_origin      2795..3250
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(2882..2901)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     3092..3113
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(3267..3286)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     3386..3408
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(3446..3464)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        3532..3636
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(3637..3705,3706..4497)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(3855..3874)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      4668..5256
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5157..5176
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt
       61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
      121 aggggttcct gcggccgcac gcgtcttgtc gagacagaga agactcttgc gtttctgata
      181 ggcacctatt ggtcttactg acatccactt tgcctttctc tccacagcag ggcgacagcc
      241 tgcacgagca catcgccaac ctggccggca gccccgccat caagaagggc atcctgcaga
      301 ccgtgaaggt ggtggacgag ctggtgaagg tgatgggccg ccacaagccc gagaacatcg
      361 tgatcgagat ggcccgcgag aaccagacca cccagaaggg ccagaagaac agccgcgagc
      421 gcatgaagcg catcgaggag ggcatcaagg agctgggcag ccagatcctg aaggagcacc
      481 ccgtggagaa cacccagctg cagaacgaga agctgtacct gtactacctg cagaacggcc
      541 gcgacatgta cgtggaccag gagctggaca tcaaccgcct gagcgactac gacgtggacc
      601 acatcgtgcc ccagagcttc ctgaaggacg acagcatcga caacaaggtg ctgacccgca
      661 gcgacaagaa ccgcggcaag agcgacaacg tgcccagcga ggaggtggtg aagaagatga
      721 agaactactg gcgccagctg ctgaacgcca agctgatcac ccagcgcaag ttcgacaacc
      781 tgaccaaggc cgagcgcggc ggcctgagcg agctggacaa ggccggcttc atcaagcgcc
      841 agctggtgga gacccgccag atcaccaagc acgtggccca gatcctggac agccgcatga
      901 acaccaagta cgacgagaac gacaagctga tccgcgaggt gaaggtgatc accctgaaga
      961 gcaagctggt gagcgacttc cgcaaggact tccagttcta caaggtgcgc gagatcaaca
     1021 actaccacca cgcccacgac gcctacctga acgccgtggt gggcaccgcc ctgatcaaga
     1081 agtaccccaa gctggagagc gagttcgtgt acggcgacta caaggtgtac gacgtgcgca
     1141 agatgatcgc caagagcgag caggagatcg gcaaggccac cgccaagtac ttcttctaca
     1201 gcaacatcat gaacttcttc aagaccgaga tcaccctggc caacggcgag atccgcaagc
     1261 gccccctgat cgagaccaac ggcgagaccg gcgagatcgt gtgggacaag ggccgcgact
     1321 tcgccaccgt gcgcaaggtg ctgagcatgc cccaggtgaa catcgtgaag aagaccgagg
     1381 tgcagaccgg cggcttcagc aaggagagca tcctgcccaa gcgcaacagc gacaagctga
     1441 tcgcccgcaa gaaggactgg gaccccaaga agtacggcgg cttcgacagc cccaccgtgg
     1501 cctacagcgt gctggtggtg gccaaggtgg agaagggcaa gagcaagaag ctgaagagcg
     1561 tgaaggagct gctgggcatc accatcatgg agcgcagcag cttcgagaag aaccccatcg
     1621 acttcctgga ggccaagggc tacaaggagg tgaagaagga cctgatcatc aagctgccca
     1681 agtacagcct gttcgagctg gagaacggcc gcaagcgcat gctggccagc gccggcgagc
     1741 tgcagaaggg caacgagctg gccctgccca gcaagtacgt gaacttcctg tacctggcca
     1801 gccactacga gaagctgaag ggcagccccg aggacaacga gcagaagcag ctgttcgtgg
     1861 agcagcacaa gcactacctg gacgagatca tcgagcagat cagcgagttc agcaagcgcg
     1921 tgatcctggc cgacgccaac ctggacaagg tgctgagcgc ctacaacaag caccgcgaca
     1981 agcccatccg cgagcaggcc gagaacatca tccacctgtt caccctgacc aacctgggcg
     2041 cccccgccgc cttcaagtac ttcgacacca ccatcgaccg caagcgctac accagcacca
     2101 aggaggtgct ggacgccacc ctgatccacc agagcatcac cggtctgtac gagacccgca
     2161 tcgacctgag ccagctgggc ggcgacggcg gctccggacc tccaaagaaa aagagaaaag
     2221 tataccccta cgacgtgccc gactacgcct aataactcga gcatgcatct agagggccct
     2281 attctatagt gtcacctaaa tgctagagct cgctgatcag cctcgactgt gccttctagt
     2341 tgccagccat ctgttgtttg cccctccccc gtgccttcct tgaccctgga aggtgccact
     2401 cccactgtcc tttcctaata aaatgaggaa attgcatcgc attgtctgag taggtgtcat
     2461 tctattctgg ggggtggggt ggggcaggac agcaaggggg aggattggga agacaatagc
     2521 aggcatgctg gggatgcggt gggctctatg gggtaaccac gtgcggaccg agcggccgca
     2581 ggaaccccta gtgatggagt tggccactcc ctctctgcgc gctcgctcgc tcactgaggc
     2641 cgggcgacca aaggtcgccc gacgcccggg ctttgcccgg gcggcctcag tgagcgagcg
     2701 agcgcgcagc tgcctgcagg ggcgcctgat gcggtatttt ctccttacgc atctgtgcgg
     2761 tatttcacac cgcatacgtc aaagcaacca tagtacgcgc cctgtagcgg cgcattaagc
     2821 gcggcgggtg tggtggttac gcgcagcgtg accgctacac ttgccagcgc cctagcgccc
     2881 gctcctttcg ctttcttccc ttcctttctc gccacgttcg ccggctttcc ccgtcaagct
     2941 ctaaatcggg ggctcccttt agggttccga tttagtgctt tacggcacct cgaccccaaa
     3001 aaacttgatt tgggtgatgg ttcacgtagt gggccatcgc cctgatagac ggtttttcgc
     3061 cctttgacgt tggagtccac gttctttaat agtggactct tgttccaaac tggaacaaca
     3121 ctcaacccta tctcgggcta ttcttttgat ttataaggga ttttgccgat ttcggcctat
     3181 tggttaaaaa atgagctgat ttaacaaaaa tttaacgcga attttaacaa aatattaacg
     3241 tttacaattt tatggtgcac tctcagtaca atctgctctg atgccgcata gttaagccag
     3301 ccccgacacc cgccaacacc cgctgacgcg ccctgacggg cttgtctgct cccggcatcc
     3361 gcttacagac aagctgtgac cgtctccggg agctgcatgt gtcagaggtt ttcaccgtca
     3421 tcaccgaaac gcgcgagacg aaagggcctc gtgatacgcc tatttttata ggttaatgtc
     3481 atgataataa tggtttctta gacgtcaggt ggcacttttc ggggaaatgt gcgcggaacc
     3541 cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc
     3601 tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc
     3661 gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg
     3721 gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat
     3781 ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc
     3841 acttttaaag ttctgctatg tggcgcggta ttatcccgta ttgacgccgg gcaagagcaa
     3901 ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa
     3961 aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt
     4021 gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct
     4081 tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat
     4141 gaagccatac caaacgacga gcgtgacacc acgatgcctg tagcaatggc aacaacgttg
     4201 cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg
     4261 atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt
     4321 attgctgata aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg
     4381 ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg
     4441 gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg
     4501 tcagaccaag tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa
     4561 aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt
     4621 tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt
     4681 tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt
     4741 ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag
     4801 ataccaaata ctgtccttct agtgtagccg tagttaggcc accacttcaa gaactctgta
     4861 gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat
     4921 aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg
     4981 ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg
     5041 agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac
     5101 aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga
     5161 aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt
     5221 ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta
     5281 cggttcctgg ccttttgctg gccttttgct cacatgt
//
