LOCUS       sequence-212743-       12435 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian expression plasmid for Env from the DU422 HIV-1 isolate; 
            QES mutant with enhanced binding to PG16.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 12435)
  AUTHORS   Heredia JD, Park J, Choi H, Gill KS, Procko E
  TITLE     Conformational engineering of HIV-1 Env based on mutational 
            tolerance in the CD4 and PG16 bound states.
  JOURNAL   J Virol. 2019 Mar 20. pii: JVI.00219-19. doi: 10.1128/JVI.00219-19.
  PUBMED    30894475
REFERENCE   2  (bases 1 to 12435)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Virol. 
            2019 Mar 20. pii: JVI.00219-19. doi: 10.1128/JVI.00219-19."
COMMENT     Sequence Label: pCEP4-DU422gp160-QES.c03
FEATURES             Location/Qualifiers
     source          1..12435
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        86..231
                     /label=HSV TK promoter
                     /note="herpes simplex virus thymidine kinase promoter"
     CDS             274..1311
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPDRE
                     MGEAN"
     primer_bind     complement(1309..1328)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    1353..1401
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     enhancer        2055..2434
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2435..2638
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2589..2609
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     2635..2659
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     polyA_signal    5271..5405
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5295..5314)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     5349..5368
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(5548..5570)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     rep_origin      6130..7919
                     /label=oriP
                     /note="Epstein-Barr virus oriP replication origin (Yates et
                     al., 2000)"
     CDS             complement(8221..10146)
                     /codon_start=1
                     /product="Epstein-Barr nuclear antigen 1, also known as 
                     EBNA-1"
                     /label=EBNA1
                     /note="crucial for latent viral infection, and for episomal
                     amplification of vectors containing the oriP origin (Okita 
                     et al., 2013)"
                     /translation="MSDEGPGTGPGNGLGEKGDTSGPEGSGGSGPQRRGGDNHGRGRGR
                     GRGRGGGRPGAPGGSGSGPRHRDGVRRPQKRPSCIGCKGTHGGTGAGAGAGGAGAGGAG
                     AGGGAGAGGGAGGAGGAGGAGAGGGAGAGGGAGGAGGAGAGGGAGAGGGAGGAGAGGGA
                     GGAGGAGAGGGAGAGGGAGGAGAGGGAGGAGGAGAGGGAGAGGAGGAGGAGAGGAGAGG
                     GAGGAGGAGAGGAGAGGAGAGGAGAGGAGGAGAGGAGGAGAGGAGGAGAGGGAGGAGAG
                     GGAGGAGAGGAGGAGAGGAGGAGAGGAGGAGAGGGAGAGGAGAGGGGRGRGGSGGRGRG
                     GSGGRGRGGSGGRRGRGRERARGGSRERARGRGRGRGEKRPRSPSSQSSSSGSPPRRPP
                     PGRRPFFHPVGEADYFEYHQEGGPDGEPDVPPGAIEQGPADDPGEGPSTGPRGQGDGGR
                     RKKGGWFGKHRGQGGSNPKFENIAEGLRALLARSHVERTTDEGTWVAGVFVYGGSKTSL
                     YNLRRGTALAIPQCRLTPLSRLPFGMAPGPGPQPGPLRESIVCYFMVFLQTHIFAEVLK
                     DAIKDLVMTKPAPTCNIRVTVCSFDDGVDLPPWFPPMVEGAAAEGDDGDDGDEGGDGDE
                     GEEGQE"
     primer_bind     complement(10581..10599)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        10667..10771
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(10772..10840,10841..11632)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(10990..11009)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      11803..12391
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     12292..12311
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 gccctgcttc atccccgtgg cccgttgctc gcgtttgctg gcggtgtccc cggaagaaat
       61 atatttgcat gtctttagtt ctatgatgac acaaaccccg cccagcgtct tgtcattggc
      121 gaattcgaac acgcagatgc agtcggggcg gcgcggtccg aggtccactt cgcatattaa
      181 ggtgacgcgt gtggcctcga acaccgagcg accctgcagc gacccgctta acagcgtcaa
      241 cagcgtgccg cagatcccgg ggggcaatga gatatgaaaa agcctgaact caccgcgacg
      301 tctgtcgaga agtttctgat cgaaaagttc gacagcgtct ccgacctgat gcagctctcg
      361 gagggcgaag aatctcgtgc tttcagcttc gatgtaggag ggcgtggata tgtcctgcgg
      421 gtaaatagct gcgccgatgg tttctacaaa gatcgttatg tttatcggca ctttgcatcg
      481 gccgcgctcc cgattccgga agtgcttgac attggggaat tcagcgagag cctgacctat
      541 tgcatctccc gccgtgcaca gggtgtcacg ttgcaagacc tgcctgaaac cgaactgccc
      601 gctgttctgc agccggtcgc ggaggccatg gatgcgatcg ctgcggccga tcttagccag
      661 acgagcgggt tcggcccatt cggaccgcaa ggaatcggtc aatacactac atggcgtgat
      721 ttcatatgcg cgattgctga tccccatgtg tatcactggc aaactgtgat ggacgacacc
      781 gtcagtgcgt ccgtcgcgca ggctctcgat gagctgatgc tttgggccga ggactgcccc
      841 gaagtccggc acctcgtgca cgcggatttc ggctccaaca atgtcctgac ggacaatggc
      901 cgcataacag cggtcattga ctggagcgag gcgatgttcg gggattccca atacgaggtc
      961 gccaacatct tcttctggag gccgtggttg gcttgtatgg agcagcagac gcgctacttc
     1021 gagcggaggc atccggagct tgcaggatcg ccgcggctcc gggcgtatat gctccgcatt
     1081 ggtcttgacc aactctatca gagcttggtt gacggcaatt tcgatgatgc agcttgggcg
     1141 cagggtcgat gcgacgcaat cgtccgatcc ggagccggga ctgtcgggcg tacacaaatc
     1201 gcccgcagaa gcgcggccgt ctggaccgat ggctgtgtag aagtactcgc cgatagtgga
     1261 aaccgacgcc ccagcactcg tccggatcgg gagatggggg aggctaactg aaacacggaa
     1321 ggagacaata ccggaaggaa cccgcgctat gacggcaata aaaagacaga ataaaacgca
     1381 cgggtgttgg gtcgtttgtt cataaacgcg gggttcggtc ccagggctgg cactctgtcg
     1441 ataccccacc gagaccccat tggggccaat acgcccgcgt ttcttccttt tccccacccc
     1501 accccccaag ttcgggtgaa ggcccagggc tcgcagccaa cgtcggggcg gcaggccctg
     1561 ccatagccac tggccccgtg ggttagggac ggggtccccc atggggaatg gtttatggtt
     1621 cgtgggggtt attattttgg gcgttgcgtg gggtcaggtc cacgactgga ctgagcagac
     1681 agacccatgg tttttggatg gcctgggcat ggaccgcatg tactggcgcg acacgaacac
     1741 cgggcgtctg tggctgccaa acacccccga cccccaaaaa ccaccgcgcg gatttctggc
     1801 gtgccaagct agtcgaccaa ttctcatgtt tgacagctta tcatcgcaga tccgggcaac
     1861 gttgttgcca ttgctgcagg cgcagaactg gtaggtatgg aagatctata cattgaatca
     1921 atattggcaa ttagccatat tagtcattgg ttatatagca taaatcaata ttggctattg
     1981 gccattgcat acgttgtatc tatatcataa tatgtacatt tatattggct catgtccaat
     2041 atgaccgcca tgttgacatt gattattgac tagttattaa tagtaatcaa ttacggggtc
     2101 attagttcat agcccatata tggagttccg cgttacataa cttacggtaa atggcccgcc
     2161 tggctgaccg cccaacgacc cccgcccatt gacgtcaata atgacgtatg ttcccatagt
     2221 aacgccaata gggactttcc attgacgtca atgggtggag tatttacggt aaactgccca
     2281 cttggcagta catcaagtgt atcatatgcc aagtccgccc cctattgacg tcaatgacgg
     2341 taaatggccc gcctggcatt atgcccagta catgacctta cgggactttc ctacttggca
     2401 gtacatctac gtattagtca tcgctattac catggtgatg cggttttggc agtacaccaa
     2461 tgggcgtgga tagcggtttg actcacgggg atttccaagt ctccacccca ttgacgtcaa
     2521 tgggagtttg ttttggcacc aaaatcaacg ggactttcca aaatgtcgta ataaccccgc
     2581 cccgttgacg caaatgggcg gtaggcgtgt acggtgggag gtctatataa gcagagctcg
     2641 tttagtgaac cgtcagatct ctagaagctg ggtaccagct gctagcgcca ccatgcccat
     2701 gggttccctc caaccacttg caacgcttta tctgctcggc atgcttgtag cgtcagtgct
     2761 tgcaaatctc gacctttggg tcaccgtata ctacggagtc cccgtgtgga aagaggctaa
     2821 gacgaccctg ttttgtgcgt cagacgcgaa agcgtatgac aaggaagtgc ataacgtgtg
     2881 ggcaacacac gcatgtgttc cgacggaccc taacccgcag gaaatcgtcc ttgaaaacgt
     2941 gaccgaaaac ttcaacatgt ggaagaacga catggttgac caaatgcacg aggacataat
     3001 cagtctttgg gatcaatcct tgaagccctg tgtgaagctg acgcctttgt gcgtaacttt
     3061 gaactgtaaa aacgtaaata tttcagcgaa tgctaacgcg accgctacac ttaattcaag
     3121 tatgaacggc gaaataaaga attgctcctt caacaccacg acagaactta gagataaaaa
     3181 gcaaaaagtc tacgcccttt tctataaacc ggatctcgtg ccacttaacg gtggtgagca
     3241 caatgagaca ggtgagtaca ttctcatcaa ctgcaattca tctacaataa cgcaagcatg
     3301 tccgaaagtt tccttcgatc cgatccctat tcactactgc gctcctgcag ggtacgccat
     3361 actgaagtgt aacaataaaa cgtttaacgg aacaggccct tgcaataatg taagtactgt
     3421 ccagtgcacg catggcataa aacccaccat gagcacacaa cttcttctta acggatcact
     3481 cgccgaggaa gagatcatcg tgcgaagtga gaacttgacg aataatataa agacgatcat
     3541 agtccatctc aacaagtccg tcgagattaa atgcactagg cccaacaaca atacgcggaa
     3601 atccgtacgg ataggtcctg gacaaacatt ctatgctacc ggtgagatta taggtgatat
     3661 acgcgaagct cattgcaata tcagtaggga gacctggaat tccactttga tccaagtgaa
     3721 agaaaaactg cgggagcatt acaacaaaac gatcaaattt gaaccttctt ctggaggtga
     3781 cctggaggtt acgacgcaca gttttaactg taggggagaa ttcttctact gcgatacgac
     3841 caagctgttc aacgagacta agctgtttaa cgagtcagaa tacgttgaca ataagactat
     3901 tatcttgccc tgccggataa agcaaattat caatatgtgg caagaagtgg gaagggcaat
     3961 gtacgcgccg ccgattgaag ggaatattac ttgcaagagc aacataactg gtcttcttct
     4021 cacatgggat ggtggagaga acagtaccga aggcgttttt aggcctggcg gtggcaatat
     4081 gaaggataac tggcgctcag aactctataa gtacaaagtc gtcgaaataa agcccttggg
     4141 cgttgcgcct acgaaaagta aacgaaaagt agtagggagg gaaaagcgag cggttggctt
     4201 gggtgcagtg cttctcgggt tcctgggagc tgcggggtca acaatgggag ctgcttccat
     4261 aaccttgact gtgcaggcaa ggcagctgct gagtgggata gtgcagcaac aatccaatct
     4321 gcttagggca attgaagctc agcaacatct tttgcagctc acagtctggg gcataaagca
     4381 actgcaaacc agagtcctcg cgattgagcg gtatcttaag gaccagcaac tgctgggact
     4441 ctgggggtgt tccgggaagc ttatttgtgc cactgcggta ccatggaact cctcttggtc
     4501 caacaagtct ctgggcgata tttgggacaa tatgacgtgg atgcagtggg atcgcgagat
     4561 atccaactac accaacacga tcttccggct tctcgaagac agtcagaatc aacaagagaa
     4621 gaatgagaag gatctgttgg cccttgacag ctggaaaaat ctttggaatt ggtttgacat
     4681 tactaactgg ctgtggtaca taaaaatctt cataatgatt gtcggtggcc ttatcggtct
     4741 ccgcatcata ttcggggtat tggctatagt caaaagggta cgccaggggt atagcccgtt
     4801 gagtttccaa acgctcattc cgaatccgcg aggtcctgat cgcttgggac ggatcgaaga
     4861 ggaaggaggt gaacaagaca aggatcgatc catacgactg gtctctgggt ttcttgccct
     4921 cgcgtgggac gacctcagat cactgtgcct gttctcttat catcaattga gggacttcat
     4981 actgaccgca gcaagagccg cagagctgct gggtcgcagt tccctgcgag gtcttcagcg
     5041 aggttgggaa gtactcaagt acctcggaaa tctggtccaa tactggggtc tcgaactgaa
     5101 gcgatccgcg ataaacctct ttgacacaat agcaattgcg gtggccgagg gaacggaccg
     5161 aataatagaa gtcatacagc gcatttgcag ggctatccga tatatcccaa cccgaattcg
     5221 acaaggtttc gaagccgcat tgttgtagta actcgaggcc ggcaaggccg gatccagaca
     5281 tgataagata cattgatgag tttggacaaa ccacaactag aatgcagtga aaaaaatgct
     5341 ttatttgtga aatttgtgat gctattgctt tatttgtaac cattataagc tgcaataaac
     5401 aagttaacaa caacaattgc attcatttta tgtttcaggt tcagggggag gtgtgggagg
     5461 ttttttaaag caagtaaaac ctctacaaat gtggtatggc tgattatgat ccggctgcct
     5521 cgcgcgtttc ggtgatgacg gtgaaaacct ctgacacatg cagctcccgg agacggtcac
     5581 agcttgtctg taagcggatg ccgggagcag acaagcccgt cagggcgcgt cagcgggtgt
     5641 tggcgggtgt cggggcgcag ccatgaggtc gactctagag gatcgatccc cgccccggac
     5701 gaactaaacc tgactacgac atctctgccc cttctacgcg gggcagtgca tgtaatccct
     5761 tcagttggtt ggtacaactt gccaactggg ccctgttcca catgtgacac ggggggggac
     5821 caaacacaaa ggggttctct gactgtagtt gacatcctta taaatggatg tgcacatttg
     5881 ccaacactga gtggctttca tcctggagca gactttgcag tctgtggact gcaacacaac
     5941 attgccttta tgtgtaactc ttggctgaag ctcttacacc aatgctgggg gacatgtacc
     6001 tcccaggggc ccaggaagac tacgggaggc tacaccaacg tcaatcagag gggcctgtgt
     6061 agctaccgat aagcggaccc tcaagagggc attagcaata gtgtttataa ggcccccttg
     6121 ttaaccctaa acgggtagca tatgcttccc gggtagtagt atatactatc cagactaacc
     6181 ctaattcaat agcatatgtt acccaacggg aagcatatgc tatcgaatta gggttagtaa
     6241 aagggtccta aggaacagcg atatctccca ccccatgagc tgtcacggtt ttatttacat
     6301 ggggtcagga ttccacgagg gtagtgaacc attttagtca caagggcagt ggctgaagat
     6361 caaggagcgg gcagtgaact ctcctgaatc ttcgcctgct tcttcattct ccttcgttta
     6421 gctaatagaa taactgctga gttgtgaaca gtaaggtgta tgtgaggtgc tcgaaaacaa
     6481 ggtttcaggt gacgccccca gaataaaatt tggacggggg gttcagtggt ggcattgtgc
     6541 tatgacacca atataaccct cacaaacccc ttgggcaata aatactagtg taggaatgaa
     6601 acattctgaa tatctttaac aatagaaatc catggggtgg ggacaagccg taaagactgg
     6661 atgtccatct cacacgaatt tatggctatg ggcaacacat aatcctagtg caatatgata
     6721 ctggggttat taagatgtgt cccaggcagg gaccaagaca ggtgaaccat gttgttacac
     6781 tctatttgta acaaggggaa agagagtgga cgccgacagc agcggactcc actggttgtc
     6841 tctaacaccc ccgaaaatta aacggggctc cacgccaatg gggcccataa acaaagacaa
     6901 gtggccactc ttttttttga aattgtggag tgggggcacg cgtcagcccc cacacgccgc
     6961 cctgcggttt tggactgtaa aataagggtg taataacttg gctgattgta accccgctaa
     7021 ccactgcggt caaaccactt gcccacaaaa ccactaatgg caccccgggg aatacctgca
     7081 taagtaggtg ggcgggccaa gataggggcg cgattgctgc gatctggagg acaaattaca
     7141 cacacttgcg cctgagcgcc aagcacaggg ttgttggtcc tcatattcac gaggtcgctg
     7201 agagcacggt gggctaatgt tgccatgggt agcatatact acccaaatat ctggatagca
     7261 tatgctatcc taatctatat ctgggtagca taggctatcc taatctatat ctgggtagca
     7321 tatgctatcc taatctatat ctgggtagta tatgctatcc taatttatat ctgggtagca
     7381 taggctatcc taatctatat ctgggtagca tatgctatcc taatctatat ctgggtagta
     7441 tatgctatcc taatctgtat ccgggtagca tatgctatcc taatagagat tagggtagta
     7501 tatgctatcc taatttatat ctgggtagca tatactaccc aaatatctgg atagcatatg
     7561 ctatcctaat ctatatctgg gtagcatatg ctatcctaat ctatatctgg gtagcatagg
     7621 ctatcctaat ctatatctgg gtagcatatg ctatcctaat ctatatctgg gtagtatatg
     7681 ctatcctaat ttatatctgg gtagcatagg ctatcctaat ctatatctgg gtagcatatg
     7741 ctatcctaat ctatatctgg gtagtatatg ctatcctaat ctgtatccgg gtagcatatg
     7801 ctatcctcat gcatatacag tcagcatatg atacccagta gtagagtggg agtgctatcc
     7861 tttgcatatg ccgccacctc ccaagggggc gtgaattttc gctgcttgtc cttttcctgc
     7921 tggttgctcc cattcttagg tgaatttaag gaggccaggc taaagccgtc gcatgtctga
     7981 ttgctcacca ggtaaatgtc gctaatgttt tccaacgcga gaaggtgttg agcgcggagc
     8041 tgagtgacgt gacaacatgg gtatgcccaa ttgccccatg ttgggaggac gaaaatggtg
     8101 acaagacaga tggccagaaa tacaccaaca gcacgcatga tgtctactgg ggatttattc
     8161 tttagtgcgg gggaatacac ggcttttaat acgattgagg gcgtctccta acaagttaca
     8221 tcactcctgc ccttcctcac cctcatctcc atcacctcct tcatctccgt catctccgtc
     8281 atcaccctcc gcggcagccc cttccaccat aggtggaaac cagggaggca aatctactcc
     8341 atcgtcaaag ctgcacacag tcaccctgat attgcaggta ggagcgggct ttgtcataac
     8401 aaggtcctta atcgcatcct tcaaaacctc agcaaatata tgagtttgta aaaagaccat
     8461 gaaataacag acaatggact cccttagcgg gccaggttgt gggccgggtc caggggccat
     8521 tccaaagggg agacgactca atggtgtaag acgacattgt ggaatagcaa gggcagttcc
     8581 tcgccttagg ttgtaaaggg aggtcttact acctccatat acgaacacac cggcgaccca
     8641 agttccttcg tcggtagtcc tttctacgtg actcctagcc aggagagctc ttaaaccttc
     8701 tgcaatgttc tcaaatttcg ggttggaacc tccttgacca cgatgctttc caaaccaccc
     8761 tccttttttg cgcctgcctc catcaccctg accccggggt ccagtgcttg ggccttctcc
     8821 tgggtcatct gcggggccct gctctatcgc tcccgggggc acgtcaggct caccatctgg
     8881 gccaccttct tggtggtatt caaaataatc ggcttcccct acagggtgga aaaatggcct
     8941 tctacctgga gggggcctgc gcggtggaga cccggatgat gatgactgac tactgggact
     9001 cctgggcctc ttttctccac gtccacgacc tctccccctg gctctttcac gacttccccc
     9061 cctggctctt tcacgtcctc taccccggcg gcctccacta cctcctcgac cccggcctcc
     9121 actacctcct cgaccccggc ctccactgcc tcctcgaccc cggcctccac ctcctgctcc
     9181 tgcccctcct gctcctgccc ctcctcctgc tcctgcccct cctgcccctc ctgctcctgc
     9241 ccctcctgcc cctcctgctc ctgcccctcc tgcccctcct gctcctgccc ctcctgcccc
     9301 tcctcctgct cctgcccctc ctgcccctcc tcctgctcct gcccctcctg cccctcctgc
     9361 tcctgcccct cctgcccctc ctgctcctgc ccctcctgcc cctcctgctc ctgcccctcc
     9421 tgctcctgcc cctcctgctc ctgcccctcc tgctcctgcc cctcctgccc ctcctgcccc
     9481 tcctcctgct cctgcccctc ctgctcctgc ccctcctgcc cctcctgccc ctcctgctcc
     9541 tgcccctcct cctgctcctg cccctcctgc ccctcctgcc cctcctcctg ctcctgcccc
     9601 tcctgcccct cctcctgctc ctgcccctcc tcctgctcct gcccctcctg cccctcctgc
     9661 ccctcctcct gctcctgccc ctcctgcccc tcctcctgct cctgcccctc ctcctgctcc
     9721 tgcccctcct gcccctcctg cccctcctcc tgctcctgcc cctcctcctg ctcctgcccc
     9781 tcctgcccct cctgcccctc ctgcccctcc tcctgctcct gcccctcctc ctgctcctgc
     9841 ccctcctgct cctgcccctc ccgctcctgc tcctgctcct gttccaccgt gggtcccttt
     9901 gcagccaatg caacttggac gtttttgggg tctccggaca ccatctctat gtcttggccc
     9961 tgatcctgag ccgcccgggg ctcctggtct tccgcctcct cgtcctcgtc ctcttccccg
    10021 tcctcgtcca tggttatcac cccctcttct ttgaggtcca ctgccgccgg agccttctgg
    10081 tccagatgtg tctcccttct ctcctaggcc atttccaggt cctgtacctg gcccctcgtc
    10141 agacatgatt cacactaaaa gagatcaata gacatcttta ttagacgacg ctcagtgaat
    10201 acagggagtg cagactcctg ccccctccaa cagccccccc accctcatcc ccttcatggt
    10261 cgctgtcaga cagatccagg tctgaaaatt ccccatcctc cgaaccatcc tcgtcctcat
    10321 caccaattac tcgcagcccg gaaaactccc gctgaacatc ctcaagattt gcgtcctgag
    10381 cctcaagcca ggcctcaaat tcctcgtccc cctttttgct ggacggtagg gatggggatt
    10441 ctcgggaccc ctcctcttcc tcttcaaggt caccagacag agatgctact ggggcaacgg
    10501 aagaaaagct gggtgcggcc tgtgaggatc agcttatcga tgataagctg tcaaacatga
    10561 gaattcttga agacgaaagg gcctcgtgat acgcctattt ttataggtta atgtcatgat
    10621 aataatggtt tcttagacgt caggtggcac ttttcgggga aatgtgcgcg gaacccctat
    10681 ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata
    10741 aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc gtgtcgccct
    10801 tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa
    10861 agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac tggatctcaa
    10921 cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttccaatga tgagcacttt
    10981 taaagttctg ctatgtggcg cggtattatc ccgtgttgac gccgggcaag agcaactcgg
    11041 tcgccgcata cactattctc agaatgactt ggttgagtac tcaccagtca cagaaaagca
    11101 tcttacggat ggcatgacag taagagaatt atgcagtgct gccataacca tgagtgataa
    11161 cactgcggcc aacttacttc tgacaacgat cggaggaccg aaggagctaa ccgctttttt
    11221 gcacaacatg ggggatcatg taactcgcct tgatcgttgg gaaccggagc tgaatgaagc
    11281 cataccaaac gacgagcgtg acaccacgat gcctgcagca atggcaacaa cgttgcgcaa
    11341 actattaact ggcgaactac ttactctagc ttcccggcaa caattaatag actggatgga
    11401 ggcggataaa gttgcaggac cacttctgcg ctcggccctt ccggctggct ggtttattgc
    11461 tgataaatct ggagccggtg agcgtgggtc tcgcggtatc attgcagcac tggggccaga
    11521 tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa ctatggatga
    11581 acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt aactgtcaga
    11641 ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat
    11701 ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt
    11761 ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct
    11821 gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc
    11881 ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc
    11941 aaatactgtc cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc
    12001 gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc
    12061 gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg
    12121 aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata
    12181 cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta
    12241 tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc
    12301 ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg
    12361 atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt
    12421 cctggccttt tgctg
//
