LOCUS       sequence-212756-        9261 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian expression plasmid for Env from the 25711 HIV-1 strain.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 9261)
  AUTHORS   Heredia JD, Park J, Choi H, Gill KS, Procko E
  TITLE     Conformational engineering of HIV-1 Env based on mutational 
            tolerance in the CD4 and PG16 bound states.
  JOURNAL   J Virol. 2019 Mar 20. pii: JVI.00219-19. doi: 10.1128/JVI.00219-19.
  PUBMED    30894475
REFERENCE   2  (bases 1 to 9261)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Virol. 
            2019 Mar 20. pii: JVI.00219-19. doi: 10.1128/JVI.00219-19."
COMMENT     Sequence Label: pCEP4-25711gp160
FEATURES             Location/Qualifiers
     source          1..9261
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        86..231
                     /label=HSV TK promoter
                     /note="herpes simplex virus thymidine kinase promoter"
     CDS             274..1311
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPDRE
                     MGEAN"
     primer_bind     complement(1309..1328)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    1353..1401
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     enhancer        2055..2434
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2435..2638
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2589..2609
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     2635..2659
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     polyA_signal    5262..5396
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5286..5305)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     5340..5359
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(5539..5561)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     rep_origin      6121..7910
                     /label=oriP
                     /note="Epstein-Barr virus oriP replication origin (Yates et
                     al., 2000)"
ORIGIN
        1 gccctgcttc atccccgtgg cccgttgctc gcgtttgctg gcggtgtccc cggaagaaat
       61 atatttgcat gtctttagtt ctatgatgac acaaaccccg cccagcgtct tgtcattggc
      121 gaattcgaac acgcagatgc agtcggggcg gcgcggtccg aggtccactt cgcatattaa
      181 ggtgacgcgt gtggcctcga acaccgagcg accctgcagc gacccgctta acagcgtcaa
      241 cagcgtgccg cagatcccgg ggggcaatga gatatgaaaa agcctgaact caccgcgacg
      301 tctgtcgaga agtttctgat cgaaaagttc gacagcgtct ccgacctgat gcagctctcg
      361 gagggcgaag aatctcgtgc tttcagcttc gatgtaggag ggcgtggata tgtcctgcgg
      421 gtaaatagct gcgccgatgg tttctacaaa gatcgttatg tttatcggca ctttgcatcg
      481 gccgcgctcc cgattccgga agtgcttgac attggggaat tcagcgagag cctgacctat
      541 tgcatctccc gccgtgcaca gggtgtcacg ttgcaagacc tgcctgaaac cgaactgccc
      601 gctgttctgc agccggtcgc ggaggccatg gatgcgatcg ctgcggccga tcttagccag
      661 acgagcgggt tcggcccatt cggaccgcaa ggaatcggtc aatacactac atggcgtgat
      721 ttcatatgcg cgattgctga tccccatgtg tatcactggc aaactgtgat ggacgacacc
      781 gtcagtgcgt ccgtcgcgca ggctctcgat gagctgatgc tttgggccga ggactgcccc
      841 gaagtccggc acctcgtgca cgcggatttc ggctccaaca atgtcctgac ggacaatggc
      901 cgcataacag cggtcattga ctggagcgag gcgatgttcg gggattccca atacgaggtc
      961 gccaacatct tcttctggag gccgtggttg gcttgtatgg agcagcagac gcgctacttc
     1021 gagcggaggc atccggagct tgcaggatcg ccgcggctcc gggcgtatat gctccgcatt
     1081 ggtcttgacc aactctatca gagcttggtt gacggcaatt tcgatgatgc agcttgggcg
     1141 cagggtcgat gcgacgcaat cgtccgatcc ggagccggga ctgtcgggcg tacacaaatc
     1201 gcccgcagaa gcgcggccgt ctggaccgat ggctgtgtag aagtactcgc cgatagtgga
     1261 aaccgacgcc ccagcactcg tccggatcgg gagatggggg aggctaactg aaacacggaa
     1321 ggagacaata ccggaaggaa cccgcgctat gacggcaata aaaagacaga ataaaacgca
     1381 cgggtgttgg gtcgtttgtt cataaacgcg gggttcggtc ccagggctgg cactctgtcg
     1441 ataccccacc gagaccccat tggggccaat acgcccgcgt ttcttccttt tccccacccc
     1501 accccccaag ttcgggtgaa ggcccagggc tcgcagccaa cgtcggggcg gcaggccctg
     1561 ccatagccac tggccccgtg ggttagggac ggggtccccc atggggaatg gtttatggtt
     1621 cgtgggggtt attattttgg gcgttgcgtg gggtcaggtc cacgactgga ctgagcagac
     1681 agacccatgg tttttggatg gcctgggcat ggaccgcatg tactggcgcg acacgaacac
     1741 cgggcgtctg tggctgccaa acacccccga cccccaaaaa ccaccgcgcg gatttctggc
     1801 gtgccaagct agtcgaccaa ttctcatgtt tgacagctta tcatcgcaga tccgggcaac
     1861 gttgttgcca ttgctgcagg cgcagaactg gtaggtatgg aagatctata cattgaatca
     1921 atattggcaa ttagccatat tagtcattgg ttatatagca taaatcaata ttggctattg
     1981 gccattgcat acgttgtatc tatatcataa tatgtacatt tatattggct catgtccaat
     2041 atgaccgcca tgttgacatt gattattgac tagttattaa tagtaatcaa ttacggggtc
     2101 attagttcat agcccatata tggagttccg cgttacataa cttacggtaa atggcccgcc
     2161 tggctgaccg cccaacgacc cccgcccatt gacgtcaata atgacgtatg ttcccatagt
     2221 aacgccaata gggactttcc attgacgtca atgggtggag tatttacggt aaactgccca
     2281 cttggcagta catcaagtgt atcatatgcc aagtccgccc cctattgacg tcaatgacgg
     2341 taaatggccc gcctggcatt atgcccagta catgacctta cgggactttc ctacttggca
     2401 gtacatctac gtattagtca tcgctattac catggtgatg cggttttggc agtacaccaa
     2461 tgggcgtgga tagcggtttg actcacgggg atttccaagt ctccacccca ttgacgtcaa
     2521 tgggagtttg ttttggcacc aaaatcaacg ggactttcca aaatgtcgta ataaccccgc
     2581 cccgttgacg caaatgggcg gtaggcgtgt acggtgggag gtctatataa gcagagctcg
     2641 tttagtgaac cgtcagatct ctagaagctg ggtaccagct gctagcgcca ccatgcctat
     2701 ggggagcctc caacccctgg ctacactcta tttgcttggt atgctcgtag ccagtgtctt
     2761 ggctaatctc tgggtcacag tatattatgg tgtacccgta tggaaggaag ctaagaccac
     2821 cctcttctgt gcgtctgacg cgaagggcta cgacaaggaa gttcacaatg tttgggctac
     2881 tcacgcatgt gttccgactg atcccaaccc gcaagagatg cctctggaga atgttaccga
     2941 gaatttcaat atgtgggaaa atgacatggt gaatcaaatg cacgaagatg tgatcagtct
     3001 gtgggatgag tccttgaagc cttgcgtcaa actgacccct ctgtgcgtaa ctttgaactg
     3061 cacggatgtt aataaaaatg tttcctcttc cgacacggat aactacaaag agactatgaa
     3121 ggagcggaaa aattgcacgt tcaatatgac gacagagctt cgcgacaaga accagaagaa
     3181 atatgccctt ttctataagc tcgacattgt cccactggat gataatgaca atgcgtcata
     3241 ccggcttata aattgcaata cgagcacgct gactcaggcc tgcccaaagg ttagttttga
     3301 ccctataccg atccactact gtgcccctgc agggtacgca attctcaaat gcaagaataa
     3361 aaccttcaat gggatagggc cgtgtaacaa ggtttcaacc gttcaatgca cgcatggaat
     3421 aaagccggtg gtatctacac aacttttgct taacgggagt cttgcggaag aggacattgt
     3481 tattcgctca gagaacataa ccgataatgc gaaaacaata atcgtacacc tcaatgagtc
     3541 cgtggagatc gtttgcatcc gccctaataa taacactcgg aaatctatta ggataggtcc
     3601 aggccaaacc ttttacgcga cgggtgatat agttggggac atccgccagg catactgcaa
     3661 tatctcagag gggaaatgga ataagactct ccagcgggta tctgagaagc tggctgagca
     3721 cttcccgaac agtacaatta acttcaatag ctcaagcggg ggtgacctgg agattacgac
     3781 gcattctttc aattgtggcg gggaattttt ctattgcaat acgtctggcc tcttcaacgg
     3841 aacctatatg aacaatgata cgaagagcaa tgacacaaag tctaacagtt catcaatcat
     3901 cacgatccca tgtcgcatta agcaaattat caacatgtgg caagaagtcg gacgcgcggt
     3961 atacgcacca cccatcgccg gtaacattac ttgtaaatcc aacattacgg gcattcttct
     4021 gactagggat ggtgggagag gcgaagaagt gaaaaatgat accgagacat ttaggccagg
     4081 agggggcaac atgaaagaca attggaggag tgagctttat aaatacaaag tagttgaaat
     4141 caagcccctc ggagtggcac caaccgccgc caagcgcaga gtcgtcgaga gagaaaagag
     4201 ggcagtagga cttggggcgg tgctccttgg atttcttggg gcagccggga gtacgatggg
     4261 cgcagcttcc atcactttga cggttcaggc gcgacaactc ctctctggaa ttgttcaaca
     4321 gcagtctaac ttgctccggg ccattgaggc gcagcagcac atgttgcagc tcaccgtgtg
     4381 gggtattaag cagctccaag ctagggtact tgccatcgag cggtatctta aggatcaaca
     4441 gctcctcgga atttgggggt gttccggtaa gcttatttgc acaacagcgg tcccctggaa
     4501 ctccagttgg tctaataaga atcaaactga gatatgggac aagatgactt ggatgcaatg
     4561 ggatagagag atttcaaatt atacggacac tatataccgc cttcttgaag atagccaaaa
     4621 ccaacaggaa aagaatgaga aggacctttt ggaacttgac aagtggcaga acctttggag
     4681 ttggttcaac ataactaact ggctctggta cataagaata ttcataatga ttgtaggggg
     4741 cttgataggc ttgcgaataa tattcgcggt tctgtcaatc gtaaaccgcg tacgacaagg
     4801 atattcaccg ttgtcttttc agaccctcgc gccgaatcct cggggattgg accgcctggg
     4861 gcggattgaa gaagagggag ggaaagagga caggaacaga tccattcgac ttgtccacgg
     4921 ttttcttgcg cttgcgtggg acgatttgcg gtcactctgt ctgtttagct accaccggct
     4981 cagagacctt atcttgctca ttgcgcgcgc tgttgaattg ctgggtcaac gcgggtggga
     5041 agcgcttaaa tatctcgcgg ggatagttca atattggggt cttgaattga aaaaatccgc
     5101 agtatcactg ttcgatacca tagcgattgc cgtcgcagag agaactgacc gaataatagg
     5161 gttgatacag ggcatctgcc gcgcaatctg caatataccc aggcggattc gacagggctt
     5221 cgaggcagct ctccagtagt aactcgaggc cggcaaggcc ggatccagac atgataagat
     5281 acattgatga gtttggacaa accacaacta gaatgcagtg aaaaaaatgc tttatttgtg
     5341 aaatttgtga tgctattgct ttatttgtaa ccattataag ctgcaataaa caagttaaca
     5401 acaacaattg cattcatttt atgtttcagg ttcaggggga ggtgtgggag gttttttaaa
     5461 gcaagtaaaa cctctacaaa tgtggtatgg ctgattatga tccggctgcc tcgcgcgttt
     5521 cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca cagcttgtct
     5581 gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg ttggcgggtg
     5641 tcggggcgca gccatgaggt cgactctaga ggatcgatcc ccgccccgga cgaactaaac
     5701 ctgactacga catctctgcc ccttcttcgc ggggcagtgc atgtaatccc ttcagttggt
     5761 tggtacaact tgccaactgg gccctgttcc acatgtgaca cgggggggga ccaaacacaa
     5821 aggggttctc tgactgtagt tgacatcctt ataaatggat gtgcacattt gccaacactg
     5881 agtggctttc atcctggagc agactttgca gtctgtggac tgcaacacaa cattgccttt
     5941 atgtgtaact cttggctgaa gctcttacac caatgctggg ggacatgtac ctcccagggg
     6001 cccaggaaga ctacgggagg ctacaccaac gtcaatcaga ggggcctgtg tagctaccga
     6061 taagcggacc ctcaagaggg cattagcaat agtgtttata aggccccctt gttaacccta
     6121 aacgggtagc atatgcttcc cgggtagtag tatatactat ccagactaac cctaattcaa
     6181 tagcatatgt tacccaacgg gaagcatatg ctatcgaatt agggttagta aaagggtcct
     6241 aaggaacagc gatatctccc accccatgag ctgtcacggt tttatttaca tggggtcagg
     6301 attccacgag ggtagtgaac cattttagtc acaagggcag tggctgaaga tcaaggagcg
     6361 ggcagtgaac tctcctgaat cttcgcctgc ttcttcattc tccttcgttt agctaataga
     6421 ataactgctg agttgtgaac agtaaggtgt atgtgaggtg ctcgaaaaca aggtttcagg
     6481 tgacgccccc agaataaaat ttggacgggg ggttcagtgg tggcattgtg ctatgacacc
     6541 aatataaccc tcacaaaccc cttgggcaat aaatactagt gtaggaatga aacattctga
     6601 atatctttaa caatagaaat ccatggggtg gggacaagcc gtaaagactg gatgtccatc
     6661 tcacacgaat ttatggctat gggcaacaca taatcctagt gcaatatgat actggggtta
     6721 ttaagatgtg tcccaggcag ggaccaagac aggtgaacca tgttgttaca ctctatttgt
     6781 aacaagggga aagagagtgg acgccgacag cagcggactc cactggttgt ctctaacacc
     6841 cccgaaaatt aaacggggct ccacgccaat ggggcccata aacaaagaca agtggccact
     6901 cttttttttg aaattgtgga gtgggggcac gcgtcagccc ccacacgccg ccctgcggtt
     6961 ttggactgta aaataagggt gtaataactt ggctgattgt aaccccgcta accactgcgg
     7021 tcaaaccact tgcccacaaa accactaatg gcaccccggg gaatacctgc ataagtaggt
     7081 gggcgggcca agataggggc gcgattgctg cgatctggag gacaaattac acacacttgc
     7141 gcctgagcgc caagcacagg gttgttggtc ctcatattca cgaggtcgct gagagcacgg
     7201 tgggctaatg ttgccatggg tagcatatac tacccaaata tctggatagc atatgctatc
     7261 ctaatctata tctgggtagc ataggctatc ctaatctata tctgggtagc atatgctatc
     7321 ctaatctata tctgggtagt atatgctatc ctaatttata tctgggtagc ataggctatc
     7381 ctaatctata tctgggtagc atatgctatc ctaatctata tctgggtagt atatgctatc
     7441 ctaatctgta tccgggtagc atatgctatc ctaatagaga ttagggtagt atatgctatc
     7501 ctaatttata tctgggtagc atatactacc caaatatctg gatagcatat gctatcctaa
     7561 tctatatctg ggtagcatat gctatcctaa tctatatctg ggtagcatag gctatcctaa
     7621 tctatatctg ggtagcatat gctatcctaa tctatatctg ggtagtatat gctatcctaa
     7681 tttatatctg ggtagcatag gctatcctaa tctatatctg ggtagcatat gctatcctaa
     7741 tctatatctg ggtagtatat gctatcctaa tctgtatccg ggtagcatat gctatcctca
     7801 tgcatataca gtcagcatat gatacccagt agtagagtgg gagtgctatc ctttgcatat
     7861 gccgccacct cccaaggggg cgtgaatttt cgctgcttgt ccttttcctg ctggttgctc
     7921 ccattcttag gtgaatttaa ggaggccagg ctaaagccgt cgcatgtctg attgctcacc
     7981 aggtaaatgt cgctaatgtt ttccaacgcg agaaggtgtt gagcgcggag ctgagtgacg
     8041 tgacaacatg ggtatgccca attgccccat gttgggagga cgaaaatggt gacaagacag
     8101 atggccagaa atacaccaac agcacgcatg atgtctactg gggatttatt ctttagtgcg
     8161 ggggaataca cggcttttaa tacgattgag ggcgtctcct aacaagttac atcactcctg
     8221 cccttcctca ccctcatctc catcacctcc ttcatctccg tcatctccgt catcaccctc
     8281 cgcggcagcc ccttccacca taggtggaaa ccagggaggc aaatctactc catcgtcaaa
     8341 gctgcacaca gtcaccctga tattgcaggt aggagcgggc tttgtcataa caaggtcctt
     8401 aatcgcatcc ttcaaaacct cagcaaatat atgagtttgt aaaaagacca tgaaataaca
     8461 gacaatggac tcccttagcg ggccaggttg tgggccgggt ccaggggcca ttccaaaggg
     8521 gagacgactc aatggtgtaa gacgacattg tggaatagca agggcagttc ctcgccttag
     8581 gttgtaaagg gaggtcttac tacctccata tacgaacaca ccggcgaccc aagttccttc
     8641 gtcggtagtc ctttctacgt gactcctagc caggagagct cttaaacctt ctgcaatgtt
     8701 ctcaaatttc gggttggaac ctccttgacc acgatgcttt ccaaaccacc ctcctttttt
     8761 gcgcctgcct ccatcaccct gaccccgggg tccagtgctt gggccttctc ctgggtcatc
     8821 tgcggggccc tgctctatcg ctcccggggg cacgtcaggc tcaccatctg ggccaccttc
     8881 ttggtggtat tcaaaataat cggcttcccc tacagggtgg aaaaatggcc ttctacctgg
     8941 agggggcctg cgcggtggag acccggatga tgatgactga ctactgggac tcctgggcct
     9001 cttttctcca cgtccacgac ctctccccct ggctctttca cgacttcccc ccctggctct
     9061 ttcacgtcct ctaccccggc ggcctccact acctcctcga ccccggcctc cactacctcc
     9121 tcgaccccgg cctccactgc ctcctcgacc ccggcctcca cctcctgctc ctgcccctcc
     9181 tgctcctgcc cctcctcctg ctcctgcccc tcctgcccct cctgctcctg cccctcctgc
     9241 ccctcctgct cctgcccctc c
//
