LOCUS       sequence-212788-        9171 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian expression plasmid for Env from the Q842.d12 HIV-1 
            isolate; QES mutant with enhanced binding to antibody PG16.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 9171)
  AUTHORS   Heredia JD, Park J, Choi H, Gill KS, Procko E
  TITLE     Conformational engineering of HIV-1 Env based on mutational 
            tolerance in the CD4 and PG16 bound states.
  JOURNAL   J Virol. 2019 Mar 20. pii: JVI.00219-19. doi: 10.1128/JVI.00219-19.
  PUBMED    30894475
REFERENCE   2  (bases 1 to 9171)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Virol. 
            2019 Mar 20. pii: JVI.00219-19. doi: 10.1128/JVI.00219-19."
COMMENT     Sequence Label: pCEP4-Q842gp160-QES.i04.I181L
FEATURES             Location/Qualifiers
     source          1..9171
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        86..231
                     /label=HSV TK promoter
                     /note="herpes simplex virus thymidine kinase promoter"
     CDS             274..1311
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPDRE
                     MGEAN"
     primer_bind     complement(1309..1328)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    1353..1401
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     enhancer        2055..2434
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2435..2638
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2589..2609
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     2635..2659
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     polyA_signal    5259..5393
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5283..5302)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     5337..5356
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(5536..5558)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     rep_origin      6118..7907
                     /label=oriP
                     /note="Epstein-Barr virus oriP replication origin (Yates et
                     al., 2000)"
ORIGIN
        1 gccctgcttc atccccgtgg cccgttgctc gcgtttgctg gcggtgtccc cggaagaaat
       61 atatttgcat gtctttagtt ctatgatgac acaaaccccg cccagcgtct tgtcattggc
      121 gaattcgaac acgcagatgc agtcggggcg gcgcggtccg aggtccactt cgcatattaa
      181 ggtgacgcgt gtggcctcga acaccgagcg accctgcagc gacccgctta acagcgtcaa
      241 cagcgtgccg cagatcccgg ggggcaatga gatatgaaaa agcctgaact caccgcgacg
      301 tctgtcgaga agtttctgat cgaaaagttc gacagcgtct ccgacctgat gcagctctcg
      361 gagggcgaag aatctcgtgc tttcagcttc gatgtaggag ggcgtggata tgtcctgcgg
      421 gtaaatagct gcgccgatgg tttctacaaa gatcgttatg tttatcggca ctttgcatcg
      481 gccgcgctcc cgattccgga agtgcttgac attggggaat tcagcgagag cctgacctat
      541 tgcatctccc gccgtgcaca gggtgtcacg ttgcaagacc tgcctgaaac cgaactgccc
      601 gctgttctgc agccggtcgc ggaggccatg gatgcgatcg ctgcggccga tcttagccag
      661 acgagcgggt tcggcccatt cggaccgcaa ggaatcggtc aatacactac atggcgtgat
      721 ttcatatgcg cgattgctga tccccatgtg tatcactggc aaactgtgat ggacgacacc
      781 gtcagtgcgt ccgtcgcgca ggctctcgat gagctgatgc tttgggccga ggactgcccc
      841 gaagtccggc acctcgtgca cgcggatttc ggctccaaca atgtcctgac ggacaatggc
      901 cgcataacag cggtcattga ctggagcgag gcgatgttcg gggattccca atacgaggtc
      961 gccaacatct tcttctggag gccgtggttg gcttgtatgg agcagcagac gcgctacttc
     1021 gagcggaggc atccggagct tgcaggatcg ccgcggctcc gggcgtatat gctccgcatt
     1081 ggtcttgacc aactctatca gagcttggtt gacggcaatt tcgatgatgc agcttgggcg
     1141 cagggtcgat gcgacgcaat cgtccgatcc ggagccggga ctgtcgggcg tacacaaatc
     1201 gcccgcagaa gcgcggccgt ctggaccgat ggctgtgtag aagtactcgc cgatagtgga
     1261 aaccgacgcc ccagcactcg tccggatcgg gagatggggg aggctaactg aaacacggaa
     1321 ggagacaata ccggaaggaa cccgcgctat gacggcaata aaaagacaga ataaaacgca
     1381 cgggtgttgg gtcgtttgtt cataaacgcg gggttcggtc ccagggctgg cactctgtcg
     1441 ataccccacc gagaccccat tggggccaat acgcccgcgt ttcttccttt tccccacccc
     1501 accccccaag ttcgggtgaa ggcccagggc tcgcagccaa cgtcggggcg gcaggccctg
     1561 ccatagccac tggccccgtg ggttagggac ggggtccccc atggggaatg gtttatggtt
     1621 cgtgggggtt attattttgg gcgttgcgtg gggtcaggtc cacgactgga ctgagcagac
     1681 agacccatgg tttttggatg gcctgggcat ggaccgcatg tactggcgcg acacgaacac
     1741 cgggcgtctg tggctgccaa acacccccga cccccaaaaa ccaccgcgcg gatttctggc
     1801 gtgccaagct agtcgaccaa ttctcatgtt tgacagctta tcatcgcaga tccgggcaac
     1861 gttgttgcca ttgctgcagg cgcagaactg gtaggtatgg aagatctata cattgaatca
     1921 atattggcaa ttagccatat tagtcattgg ttatatagca taaatcaata ttggctattg
     1981 gccattgcat acgttgtatc tatatcataa tatgtacatt tatattggct catgtccaat
     2041 atgaccgcca tgttgacatt gattattgac tagttattaa tagtaatcaa ttacggggtc
     2101 attagttcat agcccatata tggagttccg cgttacataa cttacggtaa atggcccgcc
     2161 tggctgaccg cccaacgacc cccgcccatt gacgtcaata atgacgtatg ttcccatagt
     2221 aacgccaata gggactttcc attgacgtca atgggtggag tatttacggt aaactgccca
     2281 cttggcagta catcaagtgt atcatatgcc aagtccgccc cctattgacg tcaatgacgg
     2341 taaatggccc gcctggcatt atgcccagta catgacctta cgggactttc ctacttggca
     2401 gtacatctac gtattagtca tcgctattac catggtgatg cggttttggc agtacaccaa
     2461 tgggcgtgga tagcggtttg actcacgggg atttccaagt ctccacccca ttgacgtcaa
     2521 tgggagtttg ttttggcacc aaaatcaacg ggactttcca aaatgtcgta ataaccccgc
     2581 cccgttgacg caaatgggcg gtaggcgtgt acggtgggag gtctatataa gcagagctcg
     2641 tttagtgaac cgtcagatct ctagaagctg ggtaccagct gctagcgcca ccatgccaat
     2701 gggatcactg cagcccttgg caacgctgta cttgcttggt atgcttgtgg catctgttct
     2761 tgctgtcgat aatttgtggg tcacagtgta ctacggagta cctgtatgga aagaggcgga
     2821 aactactctt ttttgcgcgt cagacgcaaa ggcatatgag actgaaaagc ataatgtatg
     2881 ggctacccat gcgtgcgtgc ccacagaccc caacccccaa gaaatccatc tggagaacgt
     2941 tactgaagaa tttaatatgt ggaagaataa tatggtggaa cagatgcata cggacattat
     3001 cagtctgtgg gatcagtctc tgaaaccatg tgttaaactc accgatctct gcgtaacctt
     3061 ggactgtaat aacgtgacga ataatggaac tagtgacatg agggaagaga taaagaattg
     3121 ctccttcaat atgacaacag aattgcgaga caagcgacaa aaagtatatt ctctgttcta
     3181 caaattggac ctggtgcaga taaatgagga tcaaggtaac agctccaata acaagtatcg
     3241 cctgataact tgcaacacgt ctgcgattac ccaagcatgt cctaaggtta catttgaacc
     3301 cattccgatt cattattgcg cacctgcagg ctatgctatc ctgaagtgca aagatgaaga
     3361 gtttaacggg atcggcccgt gcaaaaacgt atccactgtc caatgcaccc acggtattaa
     3421 gcctgttgtg tccacacagc tccttctcaa tgggtctctg gcagagaagg aggtcaaaat
     3481 aagatgtgaa aatataacga acaacgcaaa aactatcatt gtccaactgg tgaacccagt
     3541 taaaatcaat tgcacccgac ctaataataa tacgagaaag agtatccaca tcggcccagg
     3601 gcaggccttc tacgctacag gcgatataat cggagacata agacaggcgc actgcaatgt
     3661 aaatcgtacg gaatggaata atactctcca ccaggtagtt gagcagttgc gaaagcattt
     3721 taataaaacg attaactttg ctaactcaac cggaggggac ttggaaataa caacacactc
     3781 attcaattgc gggggcgaat ttttttattg taacacgact aacctgttta atagcacttg
     3841 gaaccatacg gctagtatga actcaaccga atccaatgac acaatcatcc tgccctgtcg
     3901 aataaaacaa atcatcaata tgtggcaaag agtgggccaa gcaatgtatg cacccccaat
     3961 aagaggtgtc ataaggtgtg aatccaatat aactgggttg attcttacta gggatggagg
     4021 aaacacgaac agcacccgag aaactttccg cccaggcggg ggtgatatga gggataattg
     4081 gcgatcagaa ctttacaagt ataaggtagt taagatagaa cccctcggcg ttgctcccac
     4141 aaaagccaag agaagggttg tagaacgcga aaagcgggcc gtagggattg gcgctgtatt
     4201 catcggattt ctcggagcag ctggatctac tatgggtgca gcttcaataa cattgacagt
     4261 tcaagcccgg caactccttt caggtattgt acagcagcaa agtaacctcc tgcaagcgat
     4321 tgaggctcag cagcatctcc tgaagcttac ggtgtggggt atcaaacagc ttcaagcgcg
     4381 ggtactcgcg gttgagcgat accttaagga ccaacaattg cttggaatgt ggggctgcag
     4441 cggtaaactt atatgtacaa catctgtgcc gtggaacagt tcctggagta acaaatcaca
     4501 aaatgagatt tgggacaata tgacctggtt gcagtgggat aaagaaatat caaactatac
     4561 ccagatcatt tacgatctct tggaggagtc tcaaaaccag caggagaaaa acgaacagga
     4621 cctcctcgca ttggataaat gggccaattt gtggaactgg tttgacatat ctaactggct
     4681 ttggtatata aagatattca tcatgattgt agggggtctt attggtctga ggatagtttt
     4741 cgcagtcttg tcagtcatta accgagtgcg gcagggttat tctcccttgt catttcaaac
     4801 tcacactcct aatccgaggg gactggatag gcctgagcga atagaggaag agggcggtga
     4861 gcaagacaag aaccggtcaa tcaggctcgt gtctggtttc ctggcactgg cgtgggatga
     4921 tctgaggagt ctttgtcttt tctcatacca tcggttgcgc gacttcatac tcatcgtcgc
     4981 caggaccgta gaacttttgg gacattcctc cttgaaggga cttcggctcg gatgggaggg
     5041 attgaagtac ttggggaatc tgctgagtta ttgggggcgg gaattgcgaa tctccgccac
     5101 aaatctcttg gatacgattg ctatagtaat agctggatgg acggatagag taatcgaaat
     5161 agggcagcgc ctctgtcgag catttctcaa tatccccaga agaataaggc aaggatttga
     5221 gcgcgctctg ctctagtaac tcgaggccgg caaggccgga tccagacatg ataagataca
     5281 ttgatgagtt tggacaaacc acaactagaa tgcagtgaaa aaaatgcttt atttgtgaaa
     5341 tttgtgatgc tattgcttta tttgtaacca ttataagctg caataaacaa gttaacaaca
     5401 acaattgcat tcattttatg tttcaggttc agggggaggt gtgggaggtt ttttaaagca
     5461 agtaaaacct ctacaaatgt ggtatggctg attatgatcc ggctgcctcg cgcgtttcgg
     5521 tgatgacggt gaaaacctct gacacatgca gctcccggag acggtcacag cttgtctgta
     5581 agcggatgcc gggagcagac aagcccgtca gggcgcgtca gcgggtgttg gcgggtgtcg
     5641 gggcgcagcc atgaggtcga ctctagagga tcgatccccg ccccggacga actaaacctg
     5701 actacgacat ctctgcccct tcttcgcggg gcagtgcatg taatcccttc agttggttgg
     5761 tacaacttgc caactgggcc ctgttccaca tgtgacacgg ggggggacca aacacaaagg
     5821 ggttctctga ctgtagttga catccttata aatggatgtg cacatttgcc aacactgagt
     5881 ggctttcatc ctggagcaga ctttgcagtc tgtggactgc aacacaacat tgcctttatg
     5941 tgtaactctt ggctgaagct cttacaccaa tgctggggga catgtacctc ccaggggccc
     6001 aggaagacta cgggaggcta caccaacgtc aatcagaggg gcctgtgtag ctaccgataa
     6061 gcggaccctc aagagggcat tagcaatagt gtttataagg cccccttgtt aaccctaaac
     6121 gggtagcata tgcttcccgg gtagtagtat atactatcca gactaaccct aattcaatag
     6181 catatgttac ccaacgggaa gcatatgcta tcgaattagg gttagtaaaa gggtcctaag
     6241 gaacagcgat atctcccacc ccatgagctg tcacggtttt atttacatgg ggtcaggatt
     6301 ccacgagggt agtgaaccat tttagtcaca agggcagtgg ctgaagatca aggagcgggc
     6361 agtgaactct cctgaatctt cgcctgcttc ttcattctcc ttcgtttagc taatagaata
     6421 actgctgagt tgtgaacagt aaggtgtatg tgaggtgctc gaaaacaagg tttcaggtga
     6481 cgcccccaga ataaaatttg gacggggggt tcagtggtgg cattgtgcta tgacaccaat
     6541 ataaccctca caaacccctt gggcaataaa tactagtgta ggaatgaaac attctgaata
     6601 tctttaacaa tagaaatcca tggggtgggg acaagccgta aagactggat gtccatctca
     6661 cacgaattta tggctatggg caacacataa tcctagtgca atatgatact ggggttatta
     6721 agatgtgtcc caggcaggga ccaagacagg tgaaccatgt tgttacactc tatttgtaac
     6781 aaggggaaag agagtggacg ccgacagcag cggactccac tggttgtctc taacaccccc
     6841 gaaaattaaa cggggctcca cgccaatggg gcccataaac aaagacaagt ggccactctt
     6901 ttttttgaaa ttgtggagtg ggggcacgcg tcagccccca cacgccgccc tgcggttttg
     6961 gactgtaaaa taagggtgta ataacttggc tgattgtaac cccgctaacc actgcggtca
     7021 aaccacttgc ccacaaaacc actaatggca ccccggggaa tacctgcata agtaggtggg
     7081 cgggccaaga taggggcgcg attgctgcga tctggaggac aaattacaca cacttgcgcc
     7141 tgagcgccaa gcacagggtt gttggtcctc atattcacga ggtcgctgag agcacggtgg
     7201 gctaatgttg ccatgggtag catatactac ccaaatatct ggatagcata tgctatccta
     7261 atctatatct gggtagcata ggctatccta atctatatct gggtagcata tgctatccta
     7321 atctatatct gggtagtata tgctatccta atttatatct gggtagcata ggctatccta
     7381 atctatatct gggtagcata tgctatccta atctatatct gggtagtata tgctatccta
     7441 atctgtatcc gggtagcata tgctatccta atagagatta gggtagtata tgctatccta
     7501 atttatatct gggtagcata tactacccaa atatctggat agcatatgct atcctaatct
     7561 atatctgggt agcatatgct atcctaatct atatctgggt agcataggct atcctaatct
     7621 atatctgggt agcatatgct atcctaatct atatctgggt agtatatgct atcctaattt
     7681 atatctgggt agcataggct atcctaatct atatctgggt agcatatgct atcctaatct
     7741 atatctgggt agtatatgct atcctaatct gtatccgggt agcatatgct atcctcatgc
     7801 atatacagtc agcatatgat acccagtagt agagtgggag tgctatcctt tgcatatgcc
     7861 gccacctccc aagggggcgt gaattttcgc tgcttgtcct tttcctgctg gttgctccca
     7921 ttcttaggtg aatttaagga ggccaggcta aagccgtcgc atgtctgatt gctcaccagg
     7981 taaatgtcgc taatgttttc caacgcgaga aggtgttgag cgcggagctg agtgacgtga
     8041 caacatgggt atgcccaatt gccccatgtt gggaggacga aaatggtgac aagacagatg
     8101 gccagaaata caccaacagc acgcatgatg tctactgggg atttattctt tagtgcgggg
     8161 gaatacacgg cttttaatac gattgagggc gtctcctaac aagttacatc actcctgccc
     8221 ttcctcaccc tcatctccat cacctccttc atctccgtca tctccgtcat caccctccgc
     8281 ggcagcccct tccaccatag gtggaaacca gggaggcaaa tctactccat cgtcaaagct
     8341 gcacacagtc accctgatat tgcaggtagg agcgggcttt gtcataacaa ggtccttaat
     8401 cgcatccttc aaaacctcag caaatatatg agtttgtaaa aagaccatga aataacagac
     8461 aatggactcc cttagcgggc caggttgtgg gccgggtcca ggggccattc caaaggggag
     8521 acgactcaat ggtgtaagac gacattgtgg aatagcaagg gcagttcctc gccttaggtt
     8581 gtaaagggag gtcttactac ctccatatac gaacacaccg gcgacccaag ttccttcgtc
     8641 ggtagtcctt tctacgtgac tcctagccag gagagctctt aaaccttctg caatgttctc
     8701 aaatttcggg ttggaacctc cttgaccacg atgctttcca aaccaccctc cttttttgcg
     8761 cctgcctcca tcaccctgac cccggggtcc agtgcttggg ccttctcctg ggtcatctgc
     8821 ggggccctgc tctatcgctc ccgggggcac gtcaggctca ccatctgggc caccttcttg
     8881 gtggtattca aaataatcgg cttcccctac agggtggaaa aatggccttc tacctggagg
     8941 gggcctgcgc ggtggagacc cggatgatga tgactgacta ctgggactcc tgggcctctt
     9001 ttctccacgt ccacgacctc tccccctggc tctttcacga cttccccccc tggctctttc
     9061 acgtcctcta ccccggcggc ctccactacc tcctcgaccc cggcctccac tacctcctcg
     9121 accccggcct ccactgcctc ctcgaccccg gcctccacct cctgctcctg c
//
