LOCUS       sequence-212811-       12646 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Bacterial expression of L2.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12646)
  AUTHORS   Yuzawa S, Deng K, Wang G, Baidoo EE, Northen TR, Adams PD, Katz L, 
            Keasling JD
  TITLE     Comprehensive in Vitro Analysis of Acyltransferase Domain Exchanges 
            in Modular Polyketide Synthases and Its Application for Short-Chain 
            Ketone Production.
  JOURNAL   ACS Synth Biol. 2017 Jan 20;6(1):139-147. doi: 
            10.1021/acssynbio.6b00176. Epub 2016 Sep 6.
  PUBMED    27548700
REFERENCE   2  (bases 1 to 12646)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acssynbio.6b00176"; journalName: "ACS Synth Biol"; date: 
            "2017-01-20- 20"; volume: "6"; issue: "1"; pages: "139-147"
COMMENT     Sequence Label: pSY075
FEATURES             Location/Qualifiers
     source          1..12646
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     970..989
                     /label=LacI-R
                     /note="LacI, reverse primer"
     promoter        complement(1009..1086)
                     /gene="lacI"
                     /label=lacI promoter
     primer_bind     1292..1311
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     1399..1418
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        1399..1417
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    1418..1442
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             1457..1479
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             1499..1516
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             1526..1543
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     CDS             8905..8922
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(8975..8993)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      8989..9036
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     rep_origin      9073..9528
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(9160..9179)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     9370..9391
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     CDS             complement(9620..10435)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     CDS             complement(9620..10435)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     10343..10362
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     primer_bind     10427..10446
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     rep_origin      10557..11145
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     11046..11065
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     11299..11316
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    11331..11470
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(11396..11415)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     11556..11578
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     CDS             complement(11572..11763)
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein, which maintains plasmids at low copy
                     number"
                     /label=rop
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
     CDS             complement(join(12572..12646,1..1008))
                     /codon_start=1
                     /gene="lacI"
                     /product="lac repressor"
                     /label=lacI
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL
                     NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV
                     EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH
                     EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA
                     MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC
                     YIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR
                     ALADSLMQLARQVSRLESGQ"
ORIGIN
        1 ggtttgcgta ttgggcgcca gggtggtttt tcttttcacc agtgagacgg gcaacagctg
       61 attgcccttc accgcctggc cctgagagag ttgcagcaag cggtccacgc tggtttgccc
      121 cagcaggcga aaatcctgtt tgatggtggt taacggcggg atataacatg agctgtcttc
      181 ggtatcgtcg tatcccacta ccgagatgtc cgcaccaacg cgcagcccgg actcggtaat
      241 ggcgcgcatt gcgcccagcg ccatctgatc gttggcaacc agcatcgcag tgggaacgat
      301 gccctcattc agcatttgca tggtttgttg aaaaccggac atggcactcc agtcgccttc
      361 ccgttccgct atcggctgaa tttgattgcg agtgagatat ttatgccagc cagccagacg
      421 cagacgcgcc gagacagaac ttaatgggcc cgctaacagc gcgatttgct ggtgacccaa
      481 tgcgaccaga tgctccacgc ccagtcgcgt accgtcttca tgggagaaaa taatactgtt
      541 gatgggtgtc tggtcagaga catcaagaaa taacgccgga acattagtgc aggcagcttc
      601 cacagcaatg gcatcctggt catccagcgg atagttaatg atcagcccac tgacgcgttg
      661 cgcgagaaga ttgtgcaccg ccgctttaca ggcttcgacg ccgcttcgtt ctaccatcga
      721 caccaccacg ctggcaccca gttgatcggc gcgagattta atcgccgcga caatttgcga
      781 cggcgcgtgc agggccagac tggaggtggc aacgccaatc agcaacgact gtttgcccgc
      841 cagttgttgt gccacgcggt tgggaatgta attcagctcc gccatcgccg cttccacttt
      901 ttcccgcgtt ttcgcagaaa cgtggctggc ctggttcacc acgcgggaaa cggtctgata
      961 agagacaccg gcatactctg cgacatcgta taacgttact ggtttcacat tcaccaccct
     1021 gaattgactc tcttccgggc gctatcatgc cataccgcga aaggttttgc gccattcgat
     1081 ggtgtccggg atctcgacgc tctcccttat gcgactcctg cattaggaag cagcccagta
     1141 gtaggttgag gccgttgagc accgccgccg caaggaatgg tgcatgcaag gagatggcgc
     1201 ccaacagtcc cccggccacg gggcctgcca ccatacccac gccgaaacaa gcgctcatga
     1261 gcccgaagtg gcgagcccga tcttccccat cggtgatgtc ggcgatatag gcgccagcaa
     1321 ccgcacctgt ggcgccggtg atgccggcca cgatgcgtcc ggcgtagagg atcgagatcg
     1381 atctcgatcc cgcgaaatta atacgactca ctatagggga attgtgagcg gataacaatt
     1441 cccctctaga aataattttg tttaacttta agaaggagat atacatatgg gcagcagcca
     1501 ccaccaccac caccacagca gcggcctggt cccgcgtggt tcccacatgg caggtccacc
     1561 gccgttcccg cgtcgtcgtg gcccgagcgg ccgtcgtcgt tgcggcggtc gtgctacccc
     1621 gggtagcgtc cgtgaccgta cgggtcgtcg tccggcagcc gttccgagcc gtgcagtttg
     1681 cgcagcggat ctgtgcgaag aaaatgacga cggctcgaag aatgtttctg aacatcgcgg
     1741 tagcgcaggt ggttccgtgc tgtttccacg taccggcacc gtcctgccgt gggtactgac
     1801 cggtcctggc gcagcggcgg ttcgcgcacg ctccgaagca ctgcgcacgc acctgcgtgc
     1861 gagcaccgag tggtcccctg cgggcgtcgg tcaggcgctg ctggccggta cgggtgcggg
     1921 tgccgatacc caccgtgccg ttgttctggc aggcgaccgt gcccagaccc tgaacgcatt
     1981 ggcagcgctg agcgcaggcg cagaccaccc ggcagttttc accagcactc gtgcggatgc
     2041 aagcccggct ggcccggtgt ttgtgttccc gggtcaaggc tcgcagtgga ccggtatggc
     2101 tcgtgaactg ctggactccg caccggtttt cgcgcgtaag ctgcacgact gtgcagacgc
     2161 gtttgccccg tacctgggcc acagcctgct ggatagcgtc accggtgcag caggtggtcc
     2221 agagcctgtt ggcgcggacg tcgtccaacc ggcgctgttc gccgttatgg ttgcgctgac
     2281 tgatctgtgg aacgcggctg gcgttgcacc gggtgcactg ctgggtcact ccctgggtga
     2341 actggcagcc gcgcatgtcg cgggtgttct gtccctggac gattctgctc gcgtcgtggc
     2401 gcgttggagc caagcgcagg ctacgttggc gggtcgtggt gacatggtca gcgttctgtt
     2461 gcctgcggat gaattggcgg acctgctgga ccgccgttgg ccgggtcgct tggttgtggc
     2521 ggttgaaaac ggtccaggta gcgcggtcgc gagcggtgac ctggacgctg cggcggaact
     2581 ggtcgcacac ctgaccgccg aaggtatcca cgcgcgtcgc gttgacgtgg gcctggcggc
     2641 tcacagcccg cacattgacg cgatcctgcc acgtattcgc gcggacatcg cgccgattcg
     2701 tgcgcatacg ccgagcatcc cggtttattc ggcgctgcat ggtggtgcac tggatggcac
     2761 gccaatggac gcggcgtact ggtgtcgtaa tctgcgctcc actgtacgtt tcgcggacgc
     2821 gacccgtgca gccctggagg caggccatac cacgtttgtg gaggtaagcc cacatccggt
     2881 cctgactacg gcgatggagg tgagcgcaac ccgtgccgcg cacgcagcaa ctgtcctggg
     2941 tacgctgcgc cgtggtgagg gtggtccgag ccgcttcctg gcgagcctgg ccgaactgca
     3001 tgtcagcggt ggtgatgccg atctgcgtac ggttctgccg gcgagccagg cggctggctt
     3061 gccggaaacc gttctgacgg cgggtccgcg tggcgagagc gcggatggcg actctcgtca
     3121 tgaggttctg tgcgcacgcc tggcaccgct ggacccagcg gagcgtcgtg cccagctgct
     3181 gactgttgtt cgtgaaagcg cagctgccgc gctggacggc gacgatcaag gtagcattga
     3241 cggtcgtcgc acgttccgtg acctgggtat cacgtcgctg gcagcggtgg gcatccgtga
     3301 tcgcctgcat tccgcaaccg gtctgcgtct gtctccgacc gttgtgtttg atcatccgac
     3361 cccggacgca ctggcggcac acttggacac cgaactgttc ggcacgggcg cagatgccga
     3421 gccggcacca gctgcgggtg gtcgtgcggt gccgcatgac gaaccaattg cgatcgtggg
     3481 tatggcgtgc cgttaccctg gcggcgttgg tgcaccggcc gacctgtggc gtaccgttct
     3541 ggccggtgtc gacgcagttg gtccgctgcc ggctgatcgt ggctggaata ttgcggacgg
     3601 ttacgatccg gagctggcgg gtcctggtcg ttttagccag cgtgagggcg gctttctgca
     3661 cgacgcagct gaatttgatg cggagttctt tggtattagc ccgcgtgagg cattggcgat
     3721 ggacccgcag caacgtttgg ctctggaaag cgcctgggaa gcgattgagg atgcgggtct
     3781 ggacgcccat agcctgcgtg gcagccgtac tggcgttttt ctgggcttga ttacccagga
     3841 ttatggtcct cgtgcgggtg agccgaccac gcgtgcaggt gcggtggagg gtcacctgtt
     3901 cctgggtagc actggcagcg tcgcaagcgg tcgtctgagc tataccttgg gtctggaagg
     3961 tccgtctttg acgattgata cggcatgttc gagcagcctg gtggcactgc acgaagcatg
     4021 tcaagcgctg cgtaccggtg attgcgacat ggctctgact ggtggtgtga cggtcatgcc
     4081 gagcaccggc atgttggtcg agttcagccg tcagcgtggt ctgtcgcctg acggccgttg
     4141 taaagccttt tctgcatctg ccgacggttt tggtctggcg gaaggtgtcg gtatgctggt
     4201 ggttgagcgt ctgagcgatg cgcgtcgtct gggccatcgt gtgctggcgg tggtgcgcgg
     4261 ttctgcggtt aaccaagatg gcgcgagcaa tggcctgtcg gcgcctagcg gcccagcaca
     4321 acagcgcgtt attcgccagg cgctggtcaa cgctggcgtc caagcatccc aagtggacgt
     4381 tgtcgaagca catggcaccg gtacgaaact gggcgatccg attgaggctc aagccctgca
     4441 agcgacctat ggccagggcc gtccggctga gcgcccgttg tggttgggtt ctctgaagtc
     4501 caatatcggc cacgcgcaag cggcagcggg tgtgggcggt gttatcaaaa tggtcatggc
     4561 gttgcgtgaa ggcgtcctgc caccgaccct gcacgcagac gagccgagcc cgcatattga
     4621 ctggtcggcg ggtcaggttc gtctgctgac cgaggaacgc gagtggccgg aggcaggtca
     4681 ccctcgccgt gcggcagttt cgagcttcgg tgttagcggt accaacgcgc acgtgatcat
     4741 tgaagaggcg cctgaagagg gcgaagaacc ggagtctgac gccggtggtg ttgtgccgtg
     4801 ggttttgtct gctcgtacgg aaggcgcgtt gcaggcccag gctgttcagt tgagcgagtt
     4861 cgttggtgag agctcgccgg tcgacgtggg ttggtccctg gtttctactc gtgcggcctt
     4921 cgagcatcgt gcggtggtgg ttggccgtgg tcgcgacgag ctggtacgtg gtctgagcga
     4981 ggtcgcgcag ggtcgtggtg ttcgtggcgt ggcaagcagc gcgtccggtg gtctggcttt
     5041 cgttttcgca ggccagggta gccagcgcct gggcatgggt cgcggcttgt atgagcgttt
     5101 cccggtgttt gcagaagcgt ttgatgaggt gtgtggtcgc gttggtccgg gcgtacgcga
     5161 ggttgttttt ggtagcgacg cgggtgagct ggaccgtacc gtttgggcac aagccggcct
     5221 gttcgccctg gaagtcgcgc tgtttcgtct gttggagagc tggggcgttc gtccgggttg
     5281 cctgattggc cactccgtcg gcgagctgtc cgcggcatgc gtggcgggtc tgtggagcct
     5341 ggaggacgcg tgccgcgttg tggccgctcg tgcacgtctg atgcaggccc tgccggctgg
     5401 cggcgtcatg gttgctgttc gcgcggaagc tggcgagctg gccggtttcc tgggtgagga
     5461 tgtggtgatt gcaagcgtga acgcgccagg tcaagtcgtg atcgcgggtc cggaaggcgg
     5521 cgtcgagcgt gtggttgctg catgcggtgc ccgttcgcgc cgtctggcgg tgagccatgc
     5581 gttccattcc ccgctggtgg agccgatgct gggtgagttt cgccgtgtcg ttgagagcgt
     5641 ggcatttggc gtcccgagcc tgcgtgtcgt cagcaacgtg accggtgcgt gggttgatcc
     5701 ggaagagtgg ggcaccccgg agtactgggt gcgtcaggtg cgcgaaccgg tccgttttgc
     5761 ggatggtgtc gcgacgttgc tggatgccgg cgtccgtacc tttgttgagt tgggcccagc
     5821 gggtgcgctg accagcatgg ttagccactg tgccgacgcg actgcgacgt ctgtgaccgc
     5881 cgtcccgacg ctgcgtcctg accacgacga aagccgtacg gttctgagcg ctgctgccag
     5941 cctgtatgtg cagggccatc cggttgattg ggcgcctctg ttcccacgcg cacgcaccgt
     6001 tgatctgccg acgtacgcat ttcagcatca gcgttactgg ctggatagca ccactgcaaa
     6061 cactggtgac cgtccggcag cggaccgtga caccgcattt tgggaagctg tgcagcacac
     6121 cgacctggac gccttcgctg cagaattgga cattgccccg gatgcgccgt tgggcaccgt
     6181 cttgccggct ctggctgact ggcgtcaacg cctgcgtacg gcagcggctg ttgacgcatg
     6241 gcgttaccgc accgccttta aacgtctgcc agatgcgcca ggtgcaccag tcctgacggg
     6301 cagctggctg gccgtagttc cggtgcgtca cctggatgat ccgagcgtta ccactagcct
     6361 ggatgcagtt gctaaagcgg gtgcggaagt cgttcagttg gcaatcgaag atgcggacgc
     6421 ggacgttgat cgtctgactg agcgcttgcg tggcctggtt gccggtctgg gtgccgcgcc
     6481 ggcgggcatt atgagcttcc tgggtctgga tgaagagcgt catcgtgacc acccggcgat
     6541 gccgagcggt ctggccacca gcttggcgct ggtccgcgcc ttgggtcgtg cgggcatcgg
     6601 tgcaccgctg tggatggtta cgcgtgaggc agtggcagcg ggtcaagaca cgcacccgca
     6661 tgcgcctctg ggtagcctga tctggggtct gggccaagtg acggctctgg agcacgcaga
     6721 tcgctggggt ggtctgatcg atctgccggg tgtgtgtgat gcgcgcgttg cccgcatgct
     6781 gtgcgcgggt ctgagcggcc gtggtgccga agatcagctg gccctgcgtc cgagcggcac
     6841 tttcgtccgc cgtctggcgc atatccctgg cgagcaacgt gcagcacgtc gtagctggca
     6901 accacgtggt acggtgattg tcaccggtgg tacgggtgcg ctgggtgcag tcctggcccg
     6961 ctggttggct accgaggacg cggagcacct ggtgctgacc ggccgtcgtg gcgcggacgc
     7021 ccctggcgcg gagcgtttgc gtgacgagct ggtcgctacg ggcgctcgtg tcacgctggc
     7081 ggcgtgcgac gtggcagatc gcaaagccgt cgccgcattg ctggacgaac tggctgcgga
     7141 cggcgagact gttcgcgcag ttctgcacgc tgcgggtgtc gccgatctga cgtcgctgga
     7201 gaataccggt ccagaagcgt tcgcggcagg cgtggccgcg aaggtcgatg gtgcactgca
     7261 cctgaccgaa ctgttggatc acgattcgct ggatgcgttt gtgttgttca gcagcattgc
     7321 gggtgtttgg ggttccggcg accacggcgc gtatgcggct gcgaacgcat ttctgaatgc
     7381 gttggcagag tacaatcgtg cacgcggtat cccgaccacg agcatcgcat ggggcgtttg
     7441 gaacgcgttt ggcgtcgagg gtgcaggcgg tatcagcgag gcggttgatt tggaccagct
     7501 gcatcgtcgc ggcctgccgc tgattgaacc agagctgggt ctgactgcac tgcgtcgcgc
     7561 tctagaccgt gacgaaacgg tgctgacggt tgctccggtt gcctgggagc gcttctttcc
     7621 gctgttctcc gctgcacgtc cgcgtccgtt gtttgaggac ttgccgcaag tgcgtgccct
     7681 gagcgcacct gtcccgacga cggcgggtcc ggccgtggaa ccaggtcgcc gtggtagcgg
     7741 cctgggcgat ttgcctctga cggatcgcga tagcgcgctg ctggccttgg tccgcggtga
     7801 gagcgcatcc gtgctgggtt acgagcgtcc agatcgcctg gacccggacc gtgcgctgcg
     7861 tgatgtgggt ttcgatagcc tgacggcgat ggaactgcgt aaccgtctgg ctaccgcgac
     7921 cggcctgacg ctgcctgcgg ccctggtgtt tgatcacccg accccactgg cgatcgcggc
     7981 gtatctgaaa gccgagctga cgagccagct ggactccggt acgcctgcac gtgaagctag
     8041 ctctgcactg cgtgacggct accgccaagc gggtgtgagc ggccgtgttc gtagctacct
     8101 ggatctgttg gcaggtctgt cggatttccg cgaacatttt gatggtagcg atggttttag
     8161 cctggatctg gttgatatgg cagatggtcc gggtgaggtg accgttattt gctgcgcggg
     8221 tacggctgcg atctctggtc cgcatgagtt cacccgtctg gcaggtgccc tgcgcggcat
     8281 tgcacctgtt cgtgcggtgc cgcagccggg ttacgaagaa ggcgaaccgc tgccttctag
     8341 catggcggct gttgcagctg tgcaggctga tgctgtcatt cgtacgcaag gcgataagcc
     8401 gttcgtggtt gcgggtcact cggcaggcgc gctgatggcg tacgcgctgg cgaccgaact
     8461 gctggatcgt ggtcacccgc ctcgcggtgt tgtcctgatt gatgtgtacc cgcctggtca
     8521 tcaggacgcg atgaacgcgt ggctggagga actgacggca acgttgttcg accgtgaaac
     8581 tgttcgtatg gacgacaccc gtctgaccgc gttgggtgct tacgaccgcc tgacgggtca
     8641 atggcgtcct cgcgaaaccg gtttgccgac cctgttggtt agcgcgggtg aaccaatggg
     8701 cccgtggccg gacgatagct ggaaaccgac ctggcctttc gagcacgaca ccgttgcggt
     8761 cccgggtgat catttcacga tggttcaaga acatgctgat gcgattgccc gtcacattga
     8821 cgcctggctg ggtggcggca acagctgata agcttatagg atccgaattc gagctccgtc
     8881 gacaagcttg cggccgcact cgagcaccac caccaccacc actgagatcc ggctgctaac
     8941 aaagcccgaa aggaagctga gttggctgct gccaccgctg agcaataact agcataaccc
     9001 cttggggcct ctaaacgggt cttgaggggt tttttgctga aaggaggaac tatatccgga
     9061 ttggcgaatg ggacgcgccc tgtagcggcg cattaagcgc ggcgggtgtg gtggttacgc
     9121 gcagcgtgac cgctacactt gccagcgccc tagcgcccgc tcctttcgct ttcttccctt
     9181 cctttctcgc cacgttcgcc ggctttcccc gtcaagctct aaatcggggg ctccctttag
     9241 ggttccgatt tagtgcttta cggcacctcg accccaaaaa acttgattag ggtgatggtt
     9301 cacgtagtgg gccatcgccc tgatagacgg tttttcgccc tttgacgttg gagtccacgt
     9361 tctttaatag tggactcttg ttccaaactg gaacaacact caaccctatc tcggtctatt
     9421 cttttgattt ataagggatt ttgccgattt cggcctattg gttaaaaaat gagctgattt
     9481 aacaaaaatt taacgcgaat tttaacaaaa tattaacgct tacaatttag gtggcacttt
     9541 tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta
     9601 tccgctcatg aattaattct tagaaaaact catcgagcat caaatgaaac tgcaatttat
     9661 tcatatcagg attatcaata ccatattttt gaaaaagccg tttctgtaat gaaggagaaa
     9721 actcaccgag gcagttccat aggatggcaa gatcctggta tcggtctgcg attccgactc
     9781 gtccaacatc aatacaacct attaatttcc cctcgtcaaa aataaggtta tcaagtgaga
     9841 aatcaccatg agtgacgact gaatccggtg agaatggcaa aagtttatgc atttctttcc
     9901 agacttgttc aacaggccag ccattacgct cgtcatcaaa atcactcgca tcaaccaaac
     9961 cgttattcat tcgtgattgc gcctgagcga gacgaaatac gcgatcgctg ttaaaaggac
    10021 aattacaaac aggaatcgaa tgcaaccggc gcaggaacac tgccagcgca tcaacaatat
    10081 tttcacctga atcaggatat tcttctaata cctggaatgc tgttttcccg gggatcgcag
    10141 tggtgagtaa ccatgcatca tcaggagtac ggataaaatg cttgatggtc ggaagaggca
    10201 taaattccgt cagccagttt agtctgacca tctcatctgt aacatcattg gcaacgctac
    10261 ctttgccatg tttcagaaac aactctggcg catcgggctt cccatacaat cgatagattg
    10321 tcgcacctga ttgcccgaca ttatcgcgag cccatttata cccatataaa tcagcatcca
    10381 tgttggaatt taatcgcggc ctagagcaag acgtttcccg ttgaatatgg ctcataacac
    10441 cccttgtatt actgtttatg taagcagaca gttttattgt tcatgaccaa aatcccttaa
    10501 cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga
    10561 gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg
    10621 gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc
    10681 agagcgcaga taccaaatac tgtccttcta gtgtagccgt agttaggcca ccacttcaag
    10741 aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc
    10801 agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg
    10861 cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac
    10921 accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga
    10981 aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt
    11041 ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag
    11101 cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg
    11161 gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta
    11221 tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc
    11281 agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cctgatgcgg
    11341 tattttctcc ttacgcatct gtgcggtatt tcacaccgca atggtgcact ctcagtacaa
    11401 tctgctctga tgccgcatag ttaagccagt atacactccg ctatcgctac gtgactgggt
    11461 catggctgcg ccccgacacc cgccaacacc cgctgacgcg ccctgacggg cttgtctgct
    11521 cccggcatcc gcttacagac aagctgtgac cgtctccggg agctgcatgt gtcagaggtt
    11581 ttcaccgtca tcaccgaaac gcgcgaggca gctgcggtaa agctcatcag cgtggtcgtg
    11641 aagcgattca cagatgtctg cctgttcatc cgcgtccagc tcgttgagtt tctccagaag
    11701 cgttaatgtc tggcttctga taaagcgggc catgttaagg gcggtttttt cctgtttggt
    11761 cactgatgcc tccgtgtaag ggggatttct gttcatgggg gtaatgatac cgatgaaacg
    11821 agagaggatg ctcacgatac gggttactga tgatgaacat gcccggttac tggaacgttg
    11881 tgagggtaaa caactggcgg tatggatgcg gcgggaccag agaaaaatca ctcagggtca
    11941 atgccagcgc ttcgttaata cagatgtagg tgttccacag ggtagccagc agcatcctgc
    12001 gatgcagatc cggaacataa tggtgcaggg cgctgacttc cgcgtttcca gactttacga
    12061 aacacggaaa ccgaagacca ttcatgttgt tgctcaggtc gcagacgttt tgcagcagca
    12121 gtcgcttcac gttcgctcgc gtatcggtga ttcattctgc taaccagtaa ggcaaccccg
    12181 ccagcctagc cgggtcctca acgacaggag cacgatcatg cgcacccgtg gggccgccat
    12241 gccggcgata atggcctgct tctcgccgaa acgtttggtg gcgggaccag tgacgaaggc
    12301 ttgagcgagg gcgtgcaaga ttccgaatac cgcaagcgac aggccgatca tcgtcgcgct
    12361 ccagcgaaag cggtcctcgc cgaaaatgac ccagagcgct gccggcacct gtcctacgag
    12421 ttgcatgata aagaagacag tcataagtgc ggcgacgata gtcatgcccc gcgcccaccg
    12481 gaaggagctg actgggttga aggctctcaa gggcatcggt cgagatcccg gtgcctaatg
    12541 agtgagctaa cttacattaa ttgcgttgcg ctcactgccc gctttccagt cgggaaacct
    12601 gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg agaggc
//
