LOCUS       sequence-212814-       12679 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Bacterial expression of L4.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12679)
  AUTHORS   Yuzawa S, Deng K, Wang G, Baidoo EE, Northen TR, Adams PD, Katz L, 
            Keasling JD
  TITLE     Comprehensive in Vitro Analysis of Acyltransferase Domain Exchanges 
            in Modular Polyketide Synthases and Its Application for Short-Chain 
            Ketone Production.
  JOURNAL   ACS Synth Biol. 2017 Jan 20;6(1):139-147. doi: 
            10.1021/acssynbio.6b00176. Epub 2016 Sep 6.
  PUBMED    27548700
REFERENCE   2  (bases 1 to 12679)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acssynbio.6b00176"; journalName: "ACS Synth Biol"; date: 
            "2017-01-20- 20"; volume: "6"; issue: "1"; pages: "139-147"
COMMENT     Sequence Label: pSY091
FEATURES             Location/Qualifiers
     source          1..12679
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     973..992
                     /label=LacI-R
                     /note="LacI, reverse primer"
     promoter        complement(1012..1089)
                     /gene="lacI"
                     /label=lacI promoter
     primer_bind     1295..1314
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     1402..1421
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        1402..1420
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    1421..1445
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             1460..1482
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             1502..1519
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             1529..1546
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     CDS             8941..8958
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(9011..9029)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      9025..9072
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     rep_origin      9109..9564
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(9196..9215)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     9406..9427
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     CDS             complement(9656..10471)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     CDS             complement(9656..10471)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     10379..10398
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     primer_bind     10463..10482
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     rep_origin      10593..11181
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     11082..11101
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     11335..11352
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    11367..11506
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(11432..11451)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     11592..11614
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     CDS             complement(11608..11799)
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein, which maintains plasmids at low copy
                     number"
                     /label=rop
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
     CDS             complement(join(12608..12679,1..1011))
                     /codon_start=1
                     /gene="lacI"
                     /product="lac repressor"
                     /label=lacI
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL
                     NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV
                     EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH
                     EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA
                     MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC
                     YIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR
                     ALADSLMQLARQVSRLESGQ"
ORIGIN
        1 ggcggtttgc gtattgggcg ccagggtggt ttttcttttc accagtgaga cgggcaacag
       61 ctgattgccc ttcaccgcct ggccctgaga gagttgcagc aagcggtcca cgctggtttg
      121 ccccagcagg cgaaaatcct gtttgatggt ggttaacggc gggatataac atgagctgtc
      181 ttcggtatcg tcgtatccca ctaccgagat gtccgcacca acgcgcagcc cggactcggt
      241 aatggcgcgc attgcgccca gcgccatctg atcgttggca accagcatcg cagtgggaac
      301 gatgccctca ttcagcattt gcatggtttg ttgaaaaccg gacatggcac tccagtcgcc
      361 ttcccgttcc gctatcggct gaatttgatt gcgagtgaga tatttatgcc agccagccag
      421 acgcagacgc gccgagacag aacttaatgg gcccgctaac agcgcgattt gctggtgacc
      481 caatgcgacc agatgctcca cgcccagtcg cgtaccgtct tcatgggaga aaataatact
      541 gttgatgggt gtctggtcag agacatcaag aaataacgcc ggaacattag tgcaggcagc
      601 ttccacagca atggcatcct ggtcatccag cggatagtta atgatcagcc cactgacgcg
      661 ttgcgcgaga agattgtgca ccgccgcttt acaggcttcg acgccgcttc gttctaccat
      721 cgacaccacc acgctggcac ccagttgatc ggcgcgagat ttaatcgccg cgacaatttg
      781 cgacggcgcg tgcagggcca gactggaggt ggcaacgcca atcagcaacg actgtttgcc
      841 cgccagttgt tgtgccacgc ggttgggaat gtaattcagc tccgccatcg ccgcttccac
      901 tttttcccgc gttttcgcag aaacgtggct ggcctggttc accacgcggg aaacggtctg
      961 ataagagaca ccggcatact ctgcgacatc gtataacgtt actggtttca cattcaccac
     1021 cctgaattga ctctcttccg ggcgctatca tgccataccg cgaaaggttt tgcgccattc
     1081 gatggtgtcc gggatctcga cgctctccct tatgcgactc ctgcattagg aagcagccca
     1141 gtagtaggtt gaggccgttg agcaccgccg ccgcaaggaa tggtgcatgc aaggagatgg
     1201 cgcccaacag tcccccggcc acggggcctg ccaccatacc cacgccgaaa caagcgctca
     1261 tgagcccgaa gtggcgagcc cgatcttccc catcggtgat gtcggcgata taggcgccag
     1321 caaccgcacc tgtggcgccg gtgatgccgg ccacgatgcg tccggcgtag aggatcgaga
     1381 tcgatctcga tcccgcgaaa ttaatacgac tcactatagg ggaattgtga gcggataaca
     1441 attcccctct agaaataatt ttgtttaact ttaagaagga gatatacata tgggcagcag
     1501 ccaccaccac caccaccaca gcagcggcct ggtcccgcgt ggttcccaca tggcaggtcc
     1561 accgccgttc ccgcgtcgtc gtggcccgag cggccgtcgt cgttgcggcg gtcgtgctac
     1621 cccgggtagc gtccgtgacc gtacgggtcg tcgtccggca gccgttccga gccgtgcagt
     1681 ttgcgcagcg gatctgtgcg aagaaaatga cgacggctcg aagaatgttt ctgaacatcg
     1741 cggtagcgca ggtggttccg tgctgtttcc acgtaccggc accgtcctgc cgtgggtact
     1801 gaccggtcct ggcgcagcgg cggttcgcgc acgctccgaa gcactgcgca cgcacctgcg
     1861 tgcgagcacc gagtggtccc ctgcgggcgt cggtcaggcg ctgctggccg gtacgggtgc
     1921 gggtgccgat acccaccgtg ccgttgttct ggcaggcgac cgtgcccaga ccctgaacgc
     1981 attggcagcg ctgagcgcag gcgcagacca cccggcagtt ttcaccagca ctcgtgcgga
     2041 tgcaagcccg gctggcccgg tgtttgtgtt cccgggtcaa ggctcgcagt ggaccggtat
     2101 ggctcgtgaa ctgctggact ccgcaccggt tttcgcgcgt aagctgcacg actgtgcaga
     2161 cgcgtttgcc ccgtacctgg gccacagcct gctggatagc gtcaccggtg cagcaggtgg
     2221 tccagagcct gttggcgcgg acgtcgtcca accggcgctg ttcgccgtta tggttgcgct
     2281 gactgatctg tggaacgcgg ctggcgttgc accgggtgca ctgctgggtc actccctggg
     2341 tgaactggca gccgcgcatg tcgcgggtgt tctgtccctg gacgattctg ctcgcgtcgt
     2401 ggcgcgttgg agccaagcgc aggctacgtt ggcgggtcgt ggtgacatgg tcagcgttct
     2461 gttgcctgcg gatgaattgg cggacctgct ggaccgccgt tggccgggtc gcttggttgt
     2521 ggcggttgaa aacggtccag gtagcgcggt cgcgagcggt gacctggacg ctgcggcgga
     2581 actggtcgca cacctgaccg ccgaaggtat ccacgcgcgt cgcgttgacg tgggcctggc
     2641 ggctcacagc ccgcacattg acgcgatcct gccacgtatt cgcgcggaca tcgcgccgat
     2701 tcgtgcgcat acgccgagca tcccggttta ttcggcgctg catggtggtg cactggatgg
     2761 cacgccaatg gacgcggcgt actggtgtcg taatctgcgc tccactgtac gtttcgcgga
     2821 cgcgacccgt gcagccctgg aggcaggcca taccacgttt gtggaggtaa gcccacatcc
     2881 ggtcctgact acggcgatgg aggtgagcgc aacccgtgcc gcgcacgcag caactgtcct
     2941 gggtacgctg cgccgtggtg agggtggtcc gagccgcttc ctggcgagcc tggccgaact
     3001 gcatgtcagc ggtggtgatg ccgatctgcg tacggttctg ccggcgagcc aggcggctgg
     3061 cttgccggaa accgttctga cggcgggtcc gcgtggcgag agcgcggatg gcgactctcg
     3121 tcatgaggtt ctgtgcgcac gcctggcacc gctggaccca gcggagcgtc gtgcccagct
     3181 gctgactgtt gttcgtgaaa gcgcagctgc cgcgctggac ggcgacgatc aaggtagcat
     3241 tgacggtcgt cgcacgttcc gtgacctggg tatcacgtcg ctggcagcgg tgggcatccg
     3301 tgatcgcctg cattccgcaa ccggtctgcg tctgtctccg accgttgtgt ttgatcatcc
     3361 gaccccggac gcactggcgg cacacttgga caccgaactg ttcggcacgg gcgcagatgc
     3421 cgagccggca ccagctgcgg gtggtcgtgc ggtgccgcat gacgaaccaa ttgcgatcgt
     3481 gggtatggcg tgccgttacc ctggcggcgt tggtgcaccg gccgacctgt ggcgtaccgt
     3541 tctggccggt gtcgacgcag ttggtccgct gccggctgat cgtggctgga atattgcgga
     3601 cggttacgat ccggagctgg cgggtcctgg tcgttttagc cagcgtgagg gcggctttct
     3661 gcacgacgca gctgaatttg atgcggagtt ctttggtatt agcccgcgtg aggcattggc
     3721 gatggacccg cagcaacgtt tggctctgga aagcgcctgg gaagcgattg aggatgcggg
     3781 tctggacgcc catagcctgc gtggcagccg tactggcgtt tttctgggct tgattaccca
     3841 ggattatggt cctcgtgcgg gtgagccgac cacgcgtgca ggtgcggtgg agggtcacct
     3901 gttcctgggt agcactggca gcgtcgcaag cggtcgtctg agctatacct tgggtctgga
     3961 aggtccgtct ttgacgattg atacggcatg ttcgagcagc ctggtggcac tgcacgaagc
     4021 atgtcaagcg ctgcgtaccg gtgattgcga catggctctg actggtggtg tgacggtcat
     4081 gccgagcacc ggcatgttgg tcgagttcag ccgtcagcgt ggtctgtcgc ctgacggccg
     4141 ttgtaaagcc ttttctgcat ctgccgacgg ttttggtctg gcggaaggtg tcggtatgct
     4201 ggtggttgag cgtctgagcg atgcgcgtcg tctgggccat cgtgtgctgg cggtggtgcg
     4261 cggttctgcg gttaaccaag atggcgcgag caatggcctg tcggcgccta gcggcccagc
     4321 acaacagcgc gttattcgcc aggcgctggt caacgctggc gtccaagcat cccaagtgga
     4381 cgttgtcgaa gcacatggca ccggtacgaa actgggcgat ccgattgagg ctcaagccct
     4441 gcaagcgacc tatggccagg gccgtccggc tgagcgcccg ttgtggttgg gttctctgaa
     4501 gtccaatatc ggccacgcgc aagcggcagc gggtgtgggc ggtgttatca aaatggtcat
     4561 ggcgttgcgt gaaggcgtcc tgccaccgac cctgcacgca gacgagccga gcccgcatat
     4621 tgactggtcg gcgggtcagg ttcgtctgct gaccgaggaa cgcgagtggc cggaggcagg
     4681 tcaccctcgc cgtgcggcag tttcgagctt cggtgttagc ggtaccaatg cccatgtgat
     4741 tgttgaagaa gccccagaaa gcagtgcgga tgccgttgcc gaaagtggtg ttcgtgttcc
     4801 agtgccagtg gtgccatggg tggttagcgc ccgtagtgcc gaaggcctgg cggcgcaagc
     4861 ggaacgtctg gcccgctttg tgggtgaacg cagtgatcaa gatccagtgg atattggttt
     4921 tagtctggtg cgtagtcgca gcctgctgga gcaccgtgcg gtggtgctgg gtaaaggccg
     4981 tgatgatctg gttgccggtc tggcgagtct ggcgagtgac ggtagtgcga cgggtgttgt
     5041 gagcggtgtg gcccgtggtc gtgcccgcgt tgcgttcggc tttagtggtc aaggtgccca
     5101 acgcgtgggt atgggtgccg agctggccag tgtgtaccca gtgttcgcgg aagcgctggc
     5161 ggaagtgacc ggtgccctgg gtctggaccc agaagtgttt ggtgatgtgg atcgcctggg
     5221 ccgtaccgaa gttacccaag ccgcgctgtt tgcctttgaa gttgccgtgg ttcgtctgct
     5281 ggaaagtttt ggtgtgcgcc cagatgtgct gattggtcat agtattggtg aaattgcggc
     5341 ggcctacgtt gcgggcgttt tcagcctggg tgatgccgcc gccctggttg gtgcgcgtgg
     5401 ccgtctgatg caagccctgc cagcgggtgg cgttatggtg gcggttcaag ccggcgaagc
     5461 ggaggtggtt gcggccctgg aaggttttgc cgatcgcgtg agcctggccg ccgtgaatgg
     5521 tccaagtagt gtggtggtga gtggcgaggc cgaagccgtt gagcaggttg ttgcccgtct
     5581 gggcaaggtt aaaagcaagc gtctgcgtgt tagtcatgcc tttcatagtc cactgatgga
     5641 accaatgctg gccgattttc gtcaagtggc cgaacagatt acctacaatg aaccacaact
     5701 gccggttgtt agtaatgtga gtggtcgtct ggccgaacca ggcgagctga ccacgccaga
     5761 ttactgggtt cgtcatgttc gtgaagccgt gcgttttggt gatggcgttc gcgcgctggc
     5821 cgcggacggc gtgggtgttc tggtggaagt tggtccagat agtgtgctga ccgccctggc
     5881 ccgtgaaagt ctggacggtg aagatggtct gcgcgccgtg ccactgctgc gcaaggatcg
     5941 cccagaacca gaaacgctgc tgacgggcgt tgcccaagcc tttacccatg gcgtgcaggt
     6001 ggattggcca gccctgctgc cgggtggccg tcgcgtggag ctgccgacgt acgcatttca
     6061 gcatcagcgt tactggctgg atagcaccac tgcaaacact ggtgaccgtc cggcagcgga
     6121 ccgtgacacc gcattttggg aagctgtgca gcacaccgac ctggacgcct tcgctgcaga
     6181 attggacatt gccccggatg cgccgttggg caccgtcttg ccggctctgg ctgactggcg
     6241 tcaacgcctg cgtacggcag cggctgttga cgcatggcgt taccgcaccg cctttaaacg
     6301 tctgccagat gcgccaggtg caccagtcct gacgggcagc tggctggccg tagttccggt
     6361 gcgtcacctg gatgatccga gcgttaccac tagcctggat gcagttgcta aagcgggtgc
     6421 ggaagtcgtt cagttggcaa tcgaagatgc ggacgcggac gttgatcgtc tgactgagcg
     6481 cttgcgtggc ctggttgccg gtctgggtgc cgcgccggcg ggcattatga gcttcctggg
     6541 tctggatgaa gagcgtcatc gtgaccaccc ggcgatgccg agcggtctgg ccaccagctt
     6601 ggcgctggtc cgcgccttgg gtcgtgcggg catcggtgca ccgctgtgga tggttacgcg
     6661 tgaggcagtg gcagcgggtc aagacacgca cccgcatgcg cctctgggta gcctgatctg
     6721 gggtctgggc caagtgacgg ctctggagca cgcagatcgc tggggtggtc tgatcgatct
     6781 gccgggtgtg tgtgatgcgc gcgttgcccg catgctgtgc gcgggtctga gcggccgtgg
     6841 tgccgaagat cagctggccc tgcgtccgag cggcactttc gtccgccgtc tggcgcatat
     6901 ccctggcgag caacgtgcag cacgtcgtag ctggcaacca cgtggtacgg tgattgtcac
     6961 cggtggtacg ggtgcgctgg gtgcagtcct ggcccgctgg ttggctaccg aggacgcgga
     7021 gcacctggtg ctgaccggcc gtcgtggcgc ggacgcccct ggcgcggagc gtttgcgtga
     7081 cgagctggtc gctacgggcg ctcgtgtcac gctggcggcg tgcgacgtgg cagatcgcaa
     7141 agccgtcgcc gcattgctgg acgaactggc tgcggacggc gagactgttc gcgcagttct
     7201 gcacgctgcg ggtgtcgccg atctgacgtc gctggagaat accggtccag aagcgttcgc
     7261 ggcaggcgtg gccgcgaagg tcgatggtgc actgcacctg accgaactgt tggatcacga
     7321 ttcgctggat gcgtttgtgt tgttcagcag cattgcgggt gtttggggtt ccggcgacca
     7381 cggcgcgtat gcggctgcga acgcatttct gaatgcgttg gcagagtaca atcgtgcacg
     7441 cggtatcccg accacgagca tcgcatgggg cgtttggaac gcgtttggcg tcgagggtgc
     7501 aggcggtatc agcgaggcgg ttgatttgga ccagctgcat cgtcgcggcc tgccgctgat
     7561 tgaaccagag ctgggtctga ctgcactgcg tcgcgctcta gaccgtgacg aaacggtgct
     7621 gacggttgct ccggttgcct gggagcgctt ctttccgctg ttctccgctg cacgtccgcg
     7681 tccgttgttt gaggacttgc cgcaagtgcg tgccctgagc gcacctgtcc cgacgacggc
     7741 gggtccggcc gtggaaccag gtcgccgtgg tagcggcctg ggcgatttgc ctctgacgga
     7801 tcgcgatagc gcgctgctgg ccttggtccg cggtgagagc gcatccgtgc tgggttacga
     7861 gcgtccagat cgcctggacc cggaccgtgc gctgcgtgat gtgggtttcg atagcctgac
     7921 ggcgatggaa ctgcgtaacc gtctggctac cgcgaccggc ctgacgctgc ctgcggccct
     7981 ggtgtttgat cacccgaccc cactggcgat cgcggcgtat ctgaaagccg agctgacgag
     8041 ccagctggac tccggtacgc ctgcacgtga agctagctct gcactgcgtg acggctaccg
     8101 ccaagcgggt gtgagcggcc gtgttcgtag ctacctggat ctgttggcag gtctgtcgga
     8161 tttccgcgaa cattttgatg gtagcgatgg ttttagcctg gatctggttg atatggcaga
     8221 tggtccgggt gaggtgaccg ttatttgctg cgcgggtacg gctgcgatct ctggtccgca
     8281 tgagttcacc cgtctggcag gtgccctgcg cggcattgca cctgttcgtg cggtgccgca
     8341 gccgggttac gaagaaggcg aaccgctgcc ttctagcatg gcggctgttg cagctgtgca
     8401 ggctgatgct gtcattcgta cgcaaggcga taagccgttc gtggttgcgg gtcactcggc
     8461 aggcgcgctg atggcgtacg cgctggcgac cgaactgctg gatcgtggtc acccgcctcg
     8521 cggtgttgtc ctgattgatg tgtacccgcc tggtcatcag gacgcgatga acgcgtggct
     8581 ggaggaactg acggcaacgt tgttcgaccg tgaaactgtt cgtatggacg acacccgtct
     8641 gaccgcgttg ggtgcttacg accgcctgac gggtcaatgg cgtcctcgcg aaaccggttt
     8701 gccgaccctg ttggttagcg cgggtgaacc aatgggcccg tggccggacg atagctggaa
     8761 accgacctgg cctttcgagc acgacaccgt tgcggtcccg ggtgatcatt tcacgatggt
     8821 tcaagaacat gctgatgcga ttgcccgtca cattgacgcc tggctgggtg gcggcaacag
     8881 ctgataagct tataggatcc gaattcgagc tccgtcgaca agcttgcggc cgcactcgag
     8941 caccaccacc accaccactg agatccggct gctaacaaag cccgaaagga agctgagttg
     9001 gctgctgcca ccgctgagca ataactagca taaccccttg gggcctctaa acgggtcttg
     9061 aggggttttt tgctgaaagg aggaactata tccggattgg cgaatgggac gcgccctgta
     9121 gcggcgcatt aagcgcggcg ggtgtggtgg ttacgcgcag cgtgaccgct acacttgcca
     9181 gcgccctagc gcccgctcct ttcgctttct tcccttcctt tctcgccacg ttcgccggct
     9241 ttccccgtca agctctaaat cgggggctcc ctttagggtt ccgatttagt gctttacggc
     9301 acctcgaccc caaaaaactt gattagggtg atggttcacg tagtgggcca tcgccctgat
     9361 agacggtttt tcgccctttg acgttggagt ccacgttctt taatagtgga ctcttgttcc
     9421 aaactggaac aacactcaac cctatctcgg tctattcttt tgatttataa gggattttgc
     9481 cgatttcggc ctattggtta aaaaatgagc tgatttaaca aaaatttaac gcgaatttta
     9541 acaaaatatt aacgcttaca atttaggtgg cacttttcgg ggaaatgtgc gcggaacccc
     9601 tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgaatt aattcttaga
     9661 aaaactcatc gagcatcaaa tgaaactgca atttattcat atcaggatta tcaataccat
     9721 atttttgaaa aagccgtttc tgtaatgaag gagaaaactc accgaggcag ttccatagga
     9781 tggcaagatc ctggtatcgg tctgcgattc cgactcgtcc aacatcaata caacctatta
     9841 atttcccctc gtcaaaaata aggttatcaa gtgagaaatc accatgagtg acgactgaat
     9901 ccggtgagaa tggcaaaagt ttatgcattt ctttccagac ttgttcaaca ggccagccat
     9961 tacgctcgtc atcaaaatca ctcgcatcaa ccaaaccgtt attcattcgt gattgcgcct
    10021 gagcgagacg aaatacgcga tcgctgttaa aaggacaatt acaaacagga atcgaatgca
    10081 accggcgcag gaacactgcc agcgcatcaa caatattttc acctgaatca ggatattctt
    10141 ctaatacctg gaatgctgtt ttcccgggga tcgcagtggt gagtaaccat gcatcatcag
    10201 gagtacggat aaaatgcttg atggtcggaa gaggcataaa ttccgtcagc cagtttagtc
    10261 tgaccatctc atctgtaaca tcattggcaa cgctaccttt gccatgtttc agaaacaact
    10321 ctggcgcatc gggcttccca tacaatcgat agattgtcgc acctgattgc ccgacattat
    10381 cgcgagccca tttataccca tataaatcag catccatgtt ggaatttaat cgcggcctag
    10441 agcaagacgt ttcccgttga atatggctca taacacccct tgtattactg tttatgtaag
    10501 cagacagttt tattgttcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg
    10561 tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc
    10621 tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag
    10681 ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtc
    10741 cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac
    10801 ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc
    10861 gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt
    10921 tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt
    10981 gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc
    11041 ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt
    11101 tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca
    11161 ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt
    11221 tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt
    11281 attaccgcct ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag
    11341 tcagtgagcg aggaagcgga agagcgcctg atgcggtatt ttctccttac gcatctgtgc
    11401 ggtatttcac accgcaatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa
    11461 gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc
    11521 aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc
    11581 tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc
    11641 gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg
    11701 ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa
    11761 gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg
    11821 atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt
    11881 tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg
    11941 gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga
    12001 tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt
    12061 gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca
    12121 tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat
    12181 cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga
    12241 caggagcacg atcatgcgca cccgtggggc cgccatgccg gcgataatgg cctgcttctc
    12301 gccgaaacgt ttggtggcgg gaccagtgac gaaggcttga gcgagggcgt gcaagattcc
    12361 gaataccgca agcgacaggc cgatcatcgt cgcgctccag cgaaagcggt cctcgccgaa
    12421 aatgacccag agcgctgccg gcacctgtcc tacgagttgc atgataaaga agacagtcat
    12481 aagtgcggcg acgatagtca tgccccgcgc ccaccggaag gagctgactg ggttgaaggc
    12541 tctcaagggc atcggtcgag atcccggtgc ctaatgagtg agctaactta cattaattgc
    12601 gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc attaatgaat
    12661 cggccaacgc gcggggaga
//
